MytuD.00629.a

acyl-CoA synthetase

CENTER ID: MytuD.00629.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00629.a.A1.GE25863 Full length( MytuD.00629.a ) 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1339
RefSeq: NP_215709.1
TubercuList: Rv1193
MTB Network Portal: Rv1193
UniProt: O05295

Orthologues for Rv1193

Species % Identity % Coverage Clones Proteins Structures
marinum 85 100 1 0 0
ulcerans 85 100 0 0 0
avium 84 100 0 0 0
paratuberculosis 84 100 0 0 0
leprae 82 100 1 0 0
smegmatis 77 99 0 0 0
thermoresistibile 77 96 0 0 0
abscessus 71 100 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLASLNPAV VSAADIADAV RIDGDVLSRS DLVGAATSVA ERVAGAHRVA VLATPTASTV LAITGCLIAG VPVVPVPADV GVTERRHMLT DSGVQAWLGP LPDDPAGLPH IPVRTHARSW HRYPEPSPGA IAMVVYTSGT TGPPKGVQLS RRAIAADLDA LAEAWQWTAE DVLVHGLPLY HVHGLVLGLL GSLRFGNRFV HTGKPTPAGY AQACYEAHGT LFFGVPTVWS RVAADQAAAG ALKPARLLVS GSAALPVPVF DKLVQLTGHR PVERYGASES LITLSTRADG ERRPGWVGLP LAGVQTRLVD DDGGEVPHDG ETVGKLQVRG PTLFDGYLNQ PDATAAAFDA DSWYRTGDVA VVDGSGMHRI VGRESVDLIK SGGYRVGAGE IETVLLGHPD VAEAAVVGVP DDDLGQRIVA YVVGSANVDA DGLINFVAQQ LSVHKRPREV RIVDALPRNA LGKVLKKQLL SEG
NT Sequence
GTGCTGCTGG CCTCCCTGAA TCCTGCTGTC GTCTCCGCCG CCGATATCGC GGACGCGGTC CGCATCGACG GCGACGTGCT GAGCCGTAGC GACCTGGTCG GCGCGGCAAC GTCGGTGGCC GAGCGGGTCG CCGGTGCGCA CCGGGTCGCC GTGCTGGCCA CGCCGACCGC GTCGACGGTG CTGGCGATCA CCGGCTGCCT GATCGCCGGC GTGCCGGTTG TGCCGGTACC CGCCGATGTG GGCGTCACCG AACGCCGGCA CATGCTCACC GACTCCGGCG TCCAGGCATG GCTGGGCCCG TTGCCCGACG ACCCAGCGGG GCTGCCACAC ATCCCGGTGC GCACGCACGC GCGGTCCTGG CACCGTTATC CGGAGCCCTC ACCCGGGGCC ATCGCCATGG TGGTCTACAC GTCCGGCACC ACCGGGCCGC CCAAAGGCGT GCAGCTGAGC CGGCGGGCGA TCGCCGCCGA CCTCGATGCA TTGGCAGAGG CCTGGCAGTG GACGGCCGAG GACGTGCTGG TCCACGGTCT GCCGCTGTAT CACGTTCACG GCCTGGTGCT GGGCTTGCTC GGGTCGCTGC GGTTCGGAAA TCGCTTCGTG CACACCGGTA AACCAACGCC GGCCGGCTAC GCCCAGGCCT GTTATGAAGC GCACGGCACG TTGTTTTTTG GGGTGCCGAC GGTGTGGTCA CGAGTGGCGG CCGACCAAGC TGCCGCCGGG GCGCTCAAAC CGGCGCGGCT GCTGGTGTCC GGGAGTGCGG CACTACCCGT GCCGGTGTTC GACAAGCTGG TGCAGCTCAC CGGGCACCGG CCCGTCGAAC GCTACGGTGC TTCGGAGTCG CTGATCACCC TATCGACGCG GGCTGACGGT GAGCGTCGCC CGGGCTGGGT CGGCCTGCCG CTGGCCGGTG TGCAGACCCG ACTGGTGGAC GACGATGGCG GTGAGGTCCC GCACGACGGG GAAACCGTTG GAAAGCTTCA GGTTCGCGGT CCGACCCTGT TCGACGGCTA CCTGAATCAA CCCGATGCCA CCGCCGCGGC GTTCGACGCC GACAGCTGGT ACCGCACCGG CGACGTCGCG GTGGTCGACG GCAGTGGGAT GCACCGCATC GTGGGACGCG AGTCGGTCGA CTTGATCAAG TCGGGTGGAT ACCGGGTCGG CGCCGGTGAA ATTGAAACGG TGCTGCTCGG GCATCCGGAC GTGGCGGAGG CGGCAGTCGT CGGGGTGCCC GACGATGATC TAGGCCAGCG GATCGTTGCC TACGTAGTCG GCTCAGCGAA TGTCGATGCG GACGGGCTTA TCAACTTTGT TGCCCAACAA CTTTCGGTGC ACAAGCGCCC GCGCGAGGTG CGTATCGTAG ATGCGCTGCC GCGCAACGCG TTGGGGAAAG TGCTCAAGAA GCAGTTGCTG TCAGAAGGC
Details for MytuD.00629.a.A1.GE25863
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 51kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLASLNP AVVSAADIAD AVRIDGDVLS RSDLVGAATS VAERVAGAHR VAVLATPTAS TVLAITGCLI AGVPVVPVPA DVGVTERRHM LTDSGVQAWL GPLPDDPAGL PHIPVRTHAR SWHRYPEPSP GAIAMVVYTS GTTGPPKGVQ LSRRAIAADL DALAEAWQWT AEDVLVHGLP LYHVHGLVLG LLGSLRFGNR FVHTGKPTPA GYAQACYEAH GTLFFGVPTV WSRVAADQAA AGALKPARLL VSGSAALPVP VFDKLVQLTG HRPVERYGAS ESLITLSTRA DGERRPGWVG LPLAGVQTRL VDDDGGEVPH DGETVGKLQV RGPTLFDGYL NQPDATAAAF DADSWYRTGD VAVVDGSGMH RIVGRESVDL IKSGGYRVGA GEIETVLLGH PDVAEAAVVG VPDDDLGQRI VAYVVGSANV DADGLINFVA QQLSVHKRPR EVRIVDALPR NALGKVLKKQ LLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc tgctggcctc cctgaatcct gctgtcgtct ccgccgccga tatcgcggac gcggtccgca tcgacggcga cgtgctgagc cgtagcgacc tggtcggcgc ggcaacgtcg gtggccgagc gggtcgccgg tgcgcaccgg gtcgccgtgc tggccacgcc gaccgcgtcg acggtgctgg cgatcaccgg ctgcctgatc gccggcgtgc cggttgtgcc ggtacccgcc gatgtgggcg tcaccgaacg ccggcacatg ctcaccgact ccggcgtcca ggcatggctg ggcccgttgc ccgacgaccc agcggggctg ccacacatcc cggtgcgcac gcacgcgcgg tcctggcacc gttatccgga gccctcaccc ggggccatcg ccatggtggt ctacacgtcc ggcaccaccg ggccgcccaa aggcgtgcag ctgagccggc gggcgatcgc cgccgacctc gatgcattgg cagaggcctg gcagtggacg gccgaggacg tgctggtcca cggtctgccg ctgtatcacg ttcacggcct ggtgctgggc ttgctcgggt cgctgcggtt cggaaatcgc ttcgtgcaca ccggtaaacc aacgccggcc ggctacgccc aggcctgtta tgaagcgcac ggcacgttgt tttttggggt gccgacggtg tggtcacgag tggcggccga ccaagctgcc gccggggcgc tcaaaccggc gcggctgctg gtgtccggga gtgcggcact acccgtgccg gtgttcgaca agctggtgca gctcaccggg caccggcccg tcgaacgcta cggtgcttcg gagtcgctga tcaccctatc gacgcgggct gacggtgagc gtcgcccggg ctgggtcggc ctgccgctgg ccggtgtgca gacccgactg gtggacgacg atggcggtga ggtcccgcac gacggggaaa ccgttggaaa gcttcaggtt cgcggtccga ccctgttcga cggctacctg aatcaacccg atgccaccgc cgcggcgttc gacgccgaca gctggtaccg caccggcgac gtcgcggtgg tcgacggcag tgggatgcac cgcatcgtgg gacgcgagtc ggtcgacttg atcaagtcgg gtggataccg ggtcggcgcc ggtgaaattg aaacggtgct gctcgggcat ccggacgtgg cggaggcggc agtcgtcggg gtgcccgacg atgatctagg ccagcggatc gttgcctacg tagtcggctc agcgaatgtc gatgcggacg ggcttatcaa ctttgttgcc caacaacttt cggtgcacaa gcgcccgcgc gaggtgcgta tcgtagatgc gctgccgcgc aacgcgttgg ggaaagtgct caagaagcag ttgctgtcag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLASLNP AVVSAADIAD AVRIDGDVLS RSDLVGAATS VAERVAGAHR VAVLATPTAS TVLAITGCLI AGVPVVPVPA DVGVTERRHM LTDSGVQAWL GPLPDDPAGL PHIPVRTHAR SWHRYPEPSP GAIAMVVYTS GTTGPPKGVQ LSRRAIAADL DALAEAWQWT AEDVLVHGLP LYHVHGLVLG LLGSLRFGNR FVHTGKPTPA GYAQACYEAH GTLFFGVPTV WSRVAADQAA AGALKPARLL VSGSAALPVP VFDKLVQLTG HRPVERYGAS ESLITLSTRA DGERRPGWVG LPLAGVQTRL VDDDGGEVPH DGETVGKLQV RGPTLFDGYL NQPDATAAAF DADSWYRTGD VAVVDGSGMH RIVGRESVDL IKSGGYRVGA GEIETVLLGH PDVAEAAVVG VPDDDLGQRI VAYVVGSANV DADGLINFVA QQLSVHKRPR EVRIVDALPR NALGKVLKKQ LLSEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgctg gcctccctga atcctgctgt cgtctccgcc gccgatatcg cggacgcggt ccgcatcgac ggcgacgtgc tgagccgtag cgacctggtc ggcgcggcaa cgtcggtggc cgagcgggtc gccggtgcgc accgggtcgc cgtgctggcc acgccgaccg cgtcgacggt gctggcgatc accggctgcc tgatcgccgg cgtgccggtt gtgccggtac ccgccgatgt gggcgtcacc gaacgccggc acatgctcac cgactccggc gtccaggcat ggctgggccc gttgcccgac gacccagcgg ggctgccaca catcccggtg cgcacgcacg cgcggtcctg gcaccgttat ccggagccct cacccggggc catcgccatg gtggtctaca cgtccggcac caccgggccg cccaaaggcg tgcagctgag ccggcgggcg atcgccgccg acctcgatgc attggcagag gcctggcagt ggacggccga ggacgtgctg gtccacggtc tgccgctgta tcacgttcac ggcctggtgc tgggcttgct cgggtcgctg cggttcggaa atcgcttcgt gcacaccggt aaaccaacgc cggccggcta cgcccaggcc tgttatgaag cgcacggcac gttgtttttt ggggtgccga cggtgtggtc acgagtggcg gccgaccaag ctgccgccgg ggcgctcaaa ccggcgcggc tgctggtgtc cgggagtgcg gcactacccg tgccggtgtt cgacaagctg gtgcagctca ccgggcaccg gcccgtcgaa cgctacggtg cttcggagtc gctgatcacc ctatcgacgc gggctgacgg tgagcgtcgc ccgggctggg tcggcctgcc gctggccggt gtgcagaccc gactggtgga cgacgatggc ggtgaggtcc cgcacgacgg ggaaaccgtt ggaaagcttc aggttcgcgg tccgaccctg ttcgacggct acctgaatca acccgatgcc accgccgcgg cgttcgacgc cgacagctgg taccgcaccg gcgacgtcgc ggtggtcgac ggcagtggga tgcaccgcat cgtgggacgc gagtcggtcg acttgatcaa gtcgggtgga taccgggtcg gcgccggtga aattgaaacg gtgctgctcg ggcatccgga cgtggcggag gcggcagtcg tcggggtgcc cgacgatgat ctaggccagc ggatcgttgc ctacgtagtc ggctcagcga atgtcgatgc ggacgggctt atcaactttg ttgcccaaca actttcggtg cacaagcgcc cgcgcgaggt gcgtatcgta gatgcgctgc cgcgcaacgc gttggggaaa gtgctcaaga agcagttgct gtcagaaggc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa