MyabA.00629.b

Probable fatty-acid-CoA ligase FadD (Rv0542c ortholog)

CENTER ID: MyabA.00629.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00629.b.A1.GE30324 Full length( MyabA.00629.b ) 1 463
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129266
PATRIC ID: fig|36809.5.peg.1370
RefSeq: YP_001702074.1
UniProt: B1MLJ3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLTSLHPKS PDLADAVRIG EDSWGRGDLV GAATAAAERI GGASGPIAVL ARPSIDTVLA VVAGLIAGVP IVPVPADVGI AERRHILADS AAVAWLGESP DESEGLPHVP VRRHARSWHQ YSEPNPESTA LIVYTSGTTG APKGVRLSRR ALAASLDGLA DAWEWTADDV LVHGLPLFHV HGLVLGLLGS LRVGNRFVHT VKPTPEAYAA ARGSLYFGVP TVWLRVAGDE SCARALSTAR LLVSGSAPLP VPVFDELCRL TGHAPIERYG ATETVITLST RADGERRAGW VGHPLTGVRT RLLAENGSPA AHDGETVGQL QIQGPTLFSG YHNLPEKTAE VLTEDGWYRT GDVAVIDAGG MHRIVGRESV DLIKSGGYRI GAGEIETVLL GHPGIVEVAV VGVPDDDLGQ RIVAYVVGDV AEDDVIGFVA QQLSVHKRPR EVRRVDALPR NAMGKVLKKE LIT
NT Sequence
ATGCTGCTTA CGTCGCTTCA CCCCAAGTCG CCTGATCTAG CGGACGCGGT CCGTATCGGT GAGGACAGCT GGGGGCGCGG TGACCTCGTG GGTGCTGCGA CGGCCGCCGC CGAGCGCATC GGCGGGGCTT CAGGACCGAT CGCGGTGCTC GCCCGCCCCA GCATCGACAC GGTTCTGGCG GTGGTCGCCG GACTCATCGC GGGCGTGCCG ATCGTCCCGG TACCCGCAGA CGTCGGGATT GCCGAACGAC GCCACATTCT GGCCGACAGC GCCGCGGTGG CCTGGCTGGG TGAATCCCCG GACGAGTCAG AGGGATTGCC CCACGTGCCG GTGCGCCGGC ACGCACGCTC ATGGCATCAA TATTCCGAAC CGAATCCCGA GTCGACTGCG CTCATCGTGT ATACCTCGGG AACCACCGGT GCACCGAAGG GCGTGCGACT GAGCCGGCGT GCGCTCGCTG CCAGCCTCGA CGGTCTTGCG GACGCGTGGG AATGGACTGC CGACGACGTG CTGGTGCATG GTCTTCCGCT ATTTCACGTG CACGGGCTGG TGCTCGGCCT GCTGGGTTCA TTGCGAGTGG GTAATCGGTT CGTGCATACC GTCAAGCCCA CACCAGAGGC CTACGCGGCG GCACGAGGCA GCCTGTACTT CGGCGTGCCG ACGGTATGGT TGCGGGTGGC CGGCGATGAA TCCTGTGCGC GCGCACTGTC TACCGCGCGC CTGCTGGTAT CCGGCAGTGC GCCACTGCCG GTGCCTGTGT TCGACGAGCT ATGCCGACTT ACCGGGCATG CGCCCATCGA GCGCTATGGG GCCACCGAAA CCGTCATCAC ACTTTCCACC CGTGCCGATG GAGAACGGCG CGCCGGCTGG GTTGGCCACC CGCTGACCGG TGTCCGGACC CGTCTGCTGG CAGAGAATGG GTCTCCGGCC GCCCACGACG GGGAAACCGT GGGCCAGCTG CAGATTCAGG GCCCCACATT ATTCAGTGGC TATCACAACC TGCCCGAGAA GACGGCCGAG GTACTCACGG AGGATGGCTG GTACCGCACC GGTGATGTCG CCGTCATCGA TGCCGGGGGA ATGCACCGCA TCGTCGGCCG CGAATCGGTC GACCTCATCA AGTCCGGCGG CTACCGAATC GGCGCCGGTG AAATCGAGAC CGTGCTGCTA GGGCATCCAG GCATCGTGGA AGTTGCGGTG GTGGGGGTCC CCGATGACGA CCTGGGACAA CGGATCGTCG CCTACGTTGT CGGTGATGTG GCAGAAGATG ACGTGATCGG CTTCGTGGCG CAGCAGCTTT CGGTGCACAA GCGTCCCCGT GAGGTTCGGC GGGTCGACGC GTTACCCCGG AACGCGATGG GCAAGGTCCT CAAGAAGGAG TTGATCACA
Details for MyabA.00629.b.A1.GE30324
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 95
Validated AA Sequence
MLLTSLHPKS PDLADAVRIG EDSWXRGDLX GAATAAAXRI GGASXXXAVL XRPXIXTVLA VVAGLIAGVP IVPVPAXVGI AXRRHILADS AAVAWLGESP DESEGLPHVP VRRXARSWHX YSEPNPESTA LIVYTSXTTG APXGVRLXXR ALAASLXGLA DAXXWTADXX LVHGLXLFHV HGLVLGLLGS LRVXNRFVHT VKPTPEAYAA ARGSLYXGVP TVWLRVAGDE SCARALSTAR LLVSGSAPLP VPVFDXLCRL TGHAPIERYG ATETVITLST RADGERRAGW VGHPLTGVRT RLLAENGSPA AHDGETVGQL QIQGPTLFSG YHNLPEKTAE VLTEDGWYRT GDVAVIDAGG MHRIVGRESV DLIKSGGYRI GAGEIETVLL GHPGIVEVAV VGVPDDDLGQ RIVAYVVGDV AEDDVIGFVA QQLSVHKRPR EVRRVDALPR NAMGKVLKKE LI
Validated NT Sequence
tgtgatcaac tccttcttga ggaccttgcc catcgcgttc cggggtaacg cgtcgacccg ccgaacctca cggggacgct tgtgcaccga aagctgctgc gccacgaagc cgatcacgtc atcttctgcc acatcaccga caacgtaggc gacgatccgt tgtcccaggt cgtcatcggg gacccccacc accgcaactt ccacgatgcc tggatgccct agcagcacgg tctcgatttc accggcgccg attcggtagc cgccggactt gatgaggtcg accgattcgc ggccgacgat gcggtgcatt cccccggcat cgatgacggc gacatcaccg gtgcggtacc agccatcctc cgtgagtacc tcggccgtct tctcgggcag gttgtgatag ccactgaata atgtggggcc ctgaatctgc agctggccca cggtttcccc gtcgtgggcg gccggagacc cattctctgc cagcagacgg gtccggacac cggtcagcgg gtggccaacc cagccggcgc gccgttctcc atcggcacgg gtggaaagtg tgatgacggt ttcngtggcc ccatagcgct cgatgggcgc atgcccggta agtcggcata gntcgtcgaa cacaggcacc ggcagtggcg cactgccgga taccagcagg cgcgcggtag acagtgcgcg cgcacaggat tcatcgccgg ccacccgcaa ccataccgtc ggcacgccna agtacaggct gcctcgtgcc gccgcgtagg cctctggtgt gggcttgacg gtatgcacga accgattanc cactcgcaat gaacccagca ggccgagcac cagcccgtgc acgtgaaata gcnnaagacc atgcaccagc angtngtcgg cagtccattn ncangcgtcc gcaagaccgt ngaggctggc agcgagcgca cgcnggntca gtcgcacgcc nttcggtgca ccggtggttc nngaggtata cacgatgagc gcagtcgact cgggattcgg ttcggaatat ngatgccatg agcgtgcgng ccggcgcacc ggcacgtggg gcaatccctc tgactcgtcc ggggattcac ccagccaggc caccgcggcg ctgtcggcca gaatgtggcg tcgttnggca atcccgacgt ntgcgggtac cgggacgatc ggcacgcccg cgatgagtcc ggcgaccacc gccagaaccg tgtngatgnt ggggcgggng agcaccgcgn tnnntcnnga agccccgccg atgcgntcgg cggcggcngt ngcagcaccc angaggtcac cgcgccncca gctgtcctca ccgatacgga ccgcgtccgc tagatcaggc gacttggggt gaagcgacgt aagcagcatc gaaccaggac cctgggtctg agcttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLTSLHPK SPDLADAVRI GEDSWGRGDL VGAATAAAER IGGASGPIAV LARPSIDTVL AVVAGLIAGV PIVPVPADVG IAERRHILAD SAAVAWLGES PDESEGLPHV PVRRHARSWH QYSEPNPEST ALIVYTSGTT GAPKGVRLSR RALAASLDGL ADAWEWTADD VLVHGLPLFH VHGLVLGLLG SLRVGNRFVH TVKPTPEAYA AARGSLYFGV PTVWLRVAGD ESCARALSTA RLLVSGSAPL PVPVFDELCR LTGHAPIERY GATETVITLS TRADGERRAG WVGHPLTGVR TRLLAENGSP AAHDGETVGQ LQIQGPTLFS GYHNLPEKTA EVLTEDGWYR TGDVAVIDAG GMHRIVGRES VDLIKSGGYR IGAGEIETVL LGHPGIVEVA VVGVPDDDLG QRIVAYVVGD VAEDDVIGFV AQQLSVHKRP REVRRVDALP RNAMGKVLKK ELIT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgcttacg tcgcttcacc ccaagtcgcc tgatctagcg gacgcggtcc gtatcggtga ggacagctgg gggcgcggtg acctcgtggg tgctgcgacg gccgccgccg agcgcatcgg cggggcttca ggaccgatcg cggtgctcgc ccgccccagc atcgacacgg ttctggcggt ggtcgccgga ctcatcgcgg gcgtgccgat cgtcccggta cccgcagacg tcgggattgc cgaacgacgc cacattctgg ccgacagcgc cgcggtggcc tggctgggtg aatccccgga cgagtcagag ggattgcccc acgtgccggt gcgccggcac gcacgctcat ggcatcaata ttccgaaccg aatcccgagt cgactgcgct catcgtgtat acctcgggaa ccaccggtgc accgaagggc gtgcgactga gccggcgtgc gctcgctgcc agcctcgacg gtcttgcgga cgcgtgggaa tggactgccg acgacgtgct ggtgcatggt cttccgctat ttcacgtgca cgggctggtg ctcggcctgc tgggttcatt gcgagtgggt aatcggttcg tgcataccgt caagcccaca ccagaggcct acgcggcggc acgaggcagc ctgtacttcg gcgtgccgac ggtatggttg cgggtggccg gcgatgaatc ctgtgcgcgc gcactgtcta ccgcgcgcct gctggtatcc ggcagtgcgc cactgccggt gcctgtgttc gacgagctat gccgacttac cgggcatgcg cccatcgagc gctatggggc caccgaaacc gtcatcacac tttccacccg tgccgatgga gaacggcgcg ccggctgggt tggccacccg ctgaccggtg tccggacccg tctgctggca gagaatgggt ctccggccgc ccacgacggg gaaaccgtgg gccagctgca gattcagggc cccacattat tcagtggcta tcacaacctg cccgagaaga cggccgaggt actcacggag gatggctggt accgcaccgg tgatgtcgcc gtcatcgatg ccgggggaat gcaccgcatc gtcggccgcg aatcggtcga cctcatcaag tccggcggct accgaatcgg cgccggtgaa atcgagaccg tgctgctagg gcatccaggc atcgtggaag ttgcggtggt gggggtcccc gatgacgacc tgggacaacg gatcgtcgcc tacgttgtcg gtgatgtggc agaagatgac gtgatcggct tcgtggcgca gcagctttcg gtgcacaagc gtccccgtga ggttcggcgg gtcgacgcgt taccccggaa cgcgatgggc aaggtcctca agaaggagtt gatcacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa