MyleA.00629.a

Acyl-CoA synthase

CENTER ID: MyleA.00629.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00629.a.A1.GE34937 Full length( MyleA.00629.a ) 1 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1895
RefSeq: YP_002503402.1
UniProt: A0A0H3MQA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLASLNPSV VTATDIADAV CVDGVVLSRS GLVGAATSVA ERVGGARLVA VLATPTASTV LAITGCLIAG VPVVPVPADI GVVERRHMLT DSGAQAWLGP GPSADSAPDG LPHIPVQLDA RSWNRYPEPS PDATAMVIYT SGTTGPPKGV LLSRRAIAVD LDALAQAWQW TADDVLVHGL PLFHVHGLVL GLLGSLRIGN RFVHTGKPTP TAYAQACSEA GGSLYFGVPT VWSRLVADEA VARALRPARL LVSGSASLPV PVFDRLAHLT GHRPIERYGS TESLITLSTL ADGERRAGWV GLPLAGVQTR LVDESGGPVP YDGETVGRLQ VRSPTMFGGY LNRPEATAEA FDEDGWYRTG DVAVVDSGGM HRIVGRESVD LIKLGGYRIG AGEIEMALLG HPDVREVAVV GLPDEDLGQR IVAFVVGAEA LDADELINYV AQQLSIHKRP REVRFVDALP RNAMGKVLKK QLLFEG
NT Sequence
GTGTTGTTGG CATCACTGAA TCCGTCCGTC GTTACCGCCA CCGATATCGC TGACGCGGTA TGTGTTGACG GCGTCGTGCT GAGCCGCAGC GGCTTGGTTG GTGCCGCAAC GTCGGTGGCT GAGCGTGTCG GAGGTGCACG CCTGGTAGCG GTGCTGGCCA CGCCGACGGC ATCGACTGTG CTAGCGATTA CTGGTTGCCT GATTGCCGGT GTGCCGGTGG TCCCGGTGCC CGCGGATATC GGTGTGGTCG AACGCCGGCA TATGCTCACC GATTCTGGGG CGCAGGCCTG GCTGGGTCCG GGGCCCAGTG CGGACTCGGC GCCGGACGGG TTGCCGCATA TCCCGGTTCA ACTAGATGCT CGCTCCTGGA ACCGCTATCC TGAGCCGTCA CCTGATGCCA CCGCGATGGT GATCTACACC TCGGGTACTA CTGGGCCCCC TAAGGGGGTA CTGTTGAGCC GGCGAGCGAT CGCCGTTGAT TTGGACGCGC TTGCCCAGGC CTGGCAGTGG ACGGCCGATG ACGTTTTGGT ACATGGATTG CCGCTGTTTC ACGTGCACGG CCTTGTACTC GGTTTGCTTG GATCGCTTCG GATTGGAAAT CGCTTTGTAC ACACCGGAAA ACCCACTCCG ACCGCCTATG CTCAGGCTTG CTCTGAAGCC GGGGGATCTC TGTATTTCGG CGTTCCCACG GTATGGTCGC GGCTGGTGGC AGACGAGGCA GTTGCCCGGG CGTTAAGACC CGCGCGGCTA CTAGTGTCTG GGAGTGCCTC ATTGCCGGTT CCGGTATTTG ATCGGCTAGC GCACCTCACA GGTCATCGGC CCATCGAACG GTATGGCAGC ACGGAATCAC TGATCACCCT GTCGACGTTG GCGGACGGTG AGCGCCGTGC GGGCTGGGTG GGATTGCCGC TGGCGGGCGT GCAGACCAGG CTGGTCGATG AGTCCGGAGG TCCAGTGCCC TACGACGGGG AAACCGTTGG GAGGCTTCAG GTTCGTAGTC CGACGATGTT CGGCGGCTAT CTAAATCGAC CGGAAGCGAC AGCCGAGGCG TTCGACGAGG ACGGTTGGTA CCGTACAGGT GATGTTGCGG TCGTGGACAG TGGCGGGATG CATCGCATCG TGGGTCGCGA GTCGGTTGAC CTAATCAAAT TGGGCGGATA CCGGATCGGC GCAGGAGAAA TCGAGATGGC GCTGCTAGGA CATCCGGATG TGCGGGAGGT GGCAGTTGTC GGGCTGCCTG ACGAGGATTT GGGTCAACGG ATCGTTGCAT TCGTCGTAGG CGCAGAGGCA TTAGATGCCG ATGAGTTGAT TAACTATGTT GCCCAACAGC TTTCGATACA TAAGCGGCCG CGTGAAGTCC GCTTCGTGGA CGCGCTGCCG CGCAACGCGA TGGGAAAGGT CCTCAAAAAG CAGTTGCTCT TCGAGGGC
Details for MyleA.00629.a.A1.GE34937
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLASLNP SVVTATDIAD AVCVDGVVLS RSGLVGAATS VAERVGGARL VAVLATPTAS TVLAITGCLI AGVPVVPVPA DIGVVERRHM LTDSGAQAWL GPGPSADSAP DGLPHIPVQL DARSWNRYPE PSPDATAMVI YTSGTTGPPK GVLLSRRAIA VDLDALAQAW QWTADDVLVH GLPLFHVHGL VLGLLGSLRI GNRFVHTGKP TPTAYAQACS EAGGSLYFGV PTVWSRLVAD EAVARALXPA RLLVSGSXSL PVPFDRLAHL TXHRPIERXG XTESLITLST XAXXSXXGWX XCXWXXCXPX XSXXXXVXXX XXGXXXXXXX PXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgtt gttggcatca ctgaatccgt ccgtcgttac cgccaccgat atcgctgacg cggtatgtgt tgacggcgtc gtgctgagcc gcagcggctt ggttggtgcc gcaacgtcgg tggctgagcg tgtcggaggt gcacgcctgg tagcggtgct ggccacgccg acggcatcga ctgtgctagc gattactggt tgcctgattg ccggtgtgcc ggtggtcccg gtgcccgcgg atatcggtgt ggtcgaacgc cggcatatgc tcaccgattc tggggcgcag gcctggctgg gtccggggcc cagtgcggac tcggcgccgg acgggttgcc gcatatcccg gttcaactag atgctcgctc ctggaaccgc tatcctgagc cgtcacctga tgccaccgcg atggtgatct acacctcggg tactactggg ccccctaagg gggtactgtt gagccggcga gcgatcgccg ttgatttgga cgcgcttgcc caggcctggc agtggacggc cgatgacgtt ttggtacatg gattgccgct gtttcacgtg cacggccttg tactcggttt gcttggatcg cttcggattg gaaatcgctt tgtacacacc ggaaaaccca ctccgaccgc ctatgctcag gcttgctctg aagccggggg atctctgtat ttcggcgttc ccacggtatg gtcgcggctg gtggcagacg aggcagttgc ccgggcgtta anacccgcgc ggctactagt gtctgggagt gnctcattgc cggttccatt tgatcggcta gcncacctca cagntcatcg gcccatcgaa cggnatggca ncacggaatc actgatcacc ctgtcnacnt tngcggannn gagcnctgng ggctggnngg nntgccnctg gnggncntgc nnaccnngnt ngtcnannan tcnnnangtc cnnnnnnnnn nnnngggnaa ncnntnggnn gnnnntannc cnnnnnnnnt nnnnnnnnnn nnnnannnnn ncnnannnnn nnnnnnnnnn nnnnnnnnnn nnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLASLNP SVVTATDIAD AVCVDGVVLS RSGLVGAATS VAERVGGARL VAVLATPTAS TVLAITGCLI AGVPVVPVPA DIGVVERRHM LTDSGAQAWL GPGPSADSAP DGLPHIPVQL DARSWNRYPE PSPDATAMVI YTSGTTGPPK GVLLSRRAIA VDLDALAQAW QWTADDVLVH GLPLFHVHGL VLGLLGSLRI GNRFVHTGKP TPTAYAQACS EAGGSLYFGV PTVWSRLVAD EAVARALRPA RLLVSGSASL PVPVFDRLAH LTGHRPIERY GSTESLITLS TLADGERRAG WVGLPLAGVQ TRLVDESGGP VPYDGETVGR LQVRSPTMFG GYLNRPEATA EAFDEDGWYR TGDVAVVDSG GMHRIVGRES VDLIKLGGYR IGAGEIEMAL LGHPDVREVA VVGLPDEDLG QRIVAFVVGA EALDADELIN YVAQQLSIHK RPREVRFVDA LPRNAMGKVL KKQLLFEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttgttg gcatcactga atccgtccgt cgttaccgcc accgatatcg ctgacgcggt atgtgttgac ggcgtcgtgc tgagccgcag cggcttggtt ggtgccgcaa cgtcggtggc tgagcgtgtc ggaggtgcac gcctggtagc ggtgctggcc acgccgacgg catcgactgt gctagcgatt actggttgcc tgattgccgg tgtgccggtg gtcccggtgc ccgcggatat cggtgtggtc gaacgccggc atatgctcac cgattctggg gcgcaggcct ggctgggtcc ggggcccagt gcggactcgg cgccggacgg gttgccgcat atcccggttc aactagatgc tcgctcctgg aaccgctatc ctgagccgtc acctgatgcc accgcgatgg tgatctacac ctcgggtact actgggcccc ctaagggggt actgttgagc cggcgagcga tcgccgttga tttggacgcg cttgcccagg cctggcagtg gacggccgat gacgttttgg tacatggatt gccgctgttt cacgtgcacg gccttgtact cggtttgctt ggatcgcttc ggattggaaa tcgctttgta cacaccggaa aacccactcc gaccgcctat gctcaggctt gctctgaagc cgggggatct ctgtatttcg gcgttcccac ggtatggtcg cggctggtgg cagacgaggc agttgcccgg gcgttaagac ccgcgcggct actagtgtct gggagtgcct cattgccggt tccggtattt gatcggctag cgcacctcac aggtcatcgg cccatcgaac ggtatggcag cacggaatca ctgatcaccc tgtcgacgtt ggcggacggt gagcgccgtg cgggctgggt gggattgccg ctggcgggcg tgcagaccag gctggtcgat gagtccggag gtccagtgcc ctacgacggg gaaaccgttg ggaggcttca ggttcgtagt ccgacgatgt tcggcggcta tctaaatcga ccggaagcga cagccgaggc gttcgacgag gacggttggt accgtacagg tgatgttgcg gtcgtggaca gtggcgggat gcatcgcatc gtgggtcgcg agtcggttga cctaatcaaa ttgggcggat accggatcgg cgcaggagaa atcgagatgg cgctgctagg acatccggat gtgcgggagg tggcagttgt cgggctgcct gacgaggatt tgggtcaacg gatcgttgca ttcgtcgtag gcgcagaggc attagatgcc gatgagttga ttaactatgt tgcccaacag ctttcgatac ataagcggcc gcgtgaagtc cgcttcgtgg acgcgctgcc gcgcaacgcg atgggaaagg tcctcaaaaa gcagttgctc ttcgagggca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a