MyavA.00629.a

Acyl-CoA synthase

CENTER ID: MyavA.00629.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00629.a.A1.PS00664 Full length( MyavA.00629.a ) 1 473

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1316
RefSeq: YP_880587.1
UniProt: A0A0H3A0H9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLTSLNPSA VTATDIPDAV RIDGTVLSRA DLLGAATSVA ERVAGAGRVA VLATPTAATV LAVTGCLIAG VPFVPVPADV GAAERRHMLA DSGVRAWLGP LPDEPDGLPH VPVRLHARSW HRYPEPSPDA TAMIIYTSGT TGLPKGVVLS RRAIAADLDA LAQAWQWTAD DVLVHGLPLF HVHGLVLGLL GSLRIGNRFV HTGKPTPAGY AQARTDFGGT LFFGVPTVWS RVVADEAAAR ALRPARLLVS GSAPLPVPVF DRLAGLTGHQ PVERYGASES LITISTRADG ERRPGWVGLP LTGVQTRVVD DDGNPVPHDG ETVGKLLVRG PMMFDGYLNR PDATAEAFDA DGWYRTGDVA VVDDAGMHRI VGRESVDLIK SGGYRIGAGE IETALLGHPG VAEAAVVGMP DEDLGQRIVA FVVPAGRVNP DDLIDHVAQQ LSIHKRPREV RVVDALPRNA MGKVLKKQLL SDG
NT Sequence
GTGCTGCTGA CGTCGCTGAA TCCCTCGGCC GTTACCGCCA CCGACATCCC CGACGCGGTA CGCATCGACG GCACCGTGCT CAGCCGCGCC GACCTGCTCG GCGCCGCGAC CTCGGTGGCC GAGCGGGTCG CCGGTGCGGG CCGGGTCGCC GTCCTCGCCA CACCGACCGC CGCCACGGTG CTCGCGGTGA CCGGGTGCCT GATCGCCGGG GTGCCGTTCG TCCCGGTGCC CGCCGACGTC GGGGCCGCCG AACGCCGGCA CATGCTGGCC GACTCCGGGG TGCGGGCCTG GCTCGGCCCC CTGCCTGACG AGCCCGACGG CCTGCCGCAC GTCCCGGTGC GGCTGCACGC CCGCTCCTGG CACCGCTACC CCGAGCCGTC ACCGGACGCC ACCGCGATGA TCATCTACAC CTCGGGCACC ACCGGGCTGC CCAAGGGCGT GGTGCTGAGC CGGCGCGCGA TCGCCGCCGA CCTGGACGCC CTCGCGCAGG CCTGGCAGTG GACGGCCGAC GACGTGCTGG TGCACGGGCT GCCGCTGTTT CACGTGCACG GTCTGGTGCT GGGCCTGCTG GGCTCGCTGC GGATCGGGAA TCGGTTCGTC CACACCGGAA AACCGACCCC GGCCGGCTAC GCGCAGGCCC GCACCGACTT CGGCGGCACC CTGTTCTTCG GGGTGCCGAC GGTGTGGTCG CGGGTTGTGG CCGACGAGGC GGCCGCCCGC GCGCTGCGCC CGGCGCGGCT GCTGGTCTCC GGCAGCGCGC CGCTGCCGGT CCCGGTGTTC GACCGGTTGG CCGGGCTCAC CGGGCACCAG CCCGTCGAAC GCTACGGCGC CTCGGAATCG CTGATCACCA TCTCCACCCG GGCCGACGGC GAGCGGCGCC CCGGCTGGGT GGGCCTGCCG CTGACCGGCG TGCAGACCCG GGTGGTCGAC GACGACGGCA ACCCGGTGCC GCACGACGGG GAGACCGTCG GCAAGCTGCT GGTGCGCGGC CCGATGATGT TCGACGGCTA CCTCAACCGG CCCGACGCCA CCGCCGAGGC CTTCGACGCC GACGGCTGGT ACCGCACCGG CGACGTCGCC GTCGTCGACG ACGCCGGCAT GCACCGCATC GTCGGGCGGG AGTCCGTCGA TCTGATCAAG TCCGGCGGCT ACCGCATCGG TGCCGGCGAA ATCGAAACCG CGCTGCTCGG CCACCCCGGC GTCGCCGAGG CCGCCGTCGT CGGGATGCCC GACGAGGACC TGGGGCAGCG CATCGTCGCG TTCGTCGTTC CCGCCGGCCG GGTCAACCCC GACGACCTGA TCGACCATGT CGCCCAACAA CTTTCGATAC ACAAGCGGCC CCGCGAGGTG CGCGTGGTGG ACGCGCTGCC GCGCAACGCC ATGGGCAAGG TGCTCAAGAA GCAGCTGCTG TCCGACGGC
Details for MyavA.00629.a.A1.PS00664
PURIFICATION DATe: 7/9/2010
CONCENTRATION: 34.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 26
PERCENT COVERAGE: 33
Protocol Notes
notes unavailable
Validated AA Sequence
MVDQVVGVDP AGGNDERDDA LPQVLVGHPD DGGLGDAGVA EQRGFXFAGT DAVAAGLDQI DGLPPDDAVH AGVVDDGDXA GAVPAVGVXG LGGGVGPVEV XSNIXXPRTX SLXTXSXXXX RVAXXVDHXG LXXGXXXXXX AXXXXXXXXX GXGDXXXRXA XXVXGXXX
Validated NT Sequence
tttgttagca gccggatcct cnagaagctt ggctgcagaa cttgttcgtg ctgtttatta gccgtcggac agcagctgct tcttgagcac cttgcccatg gcgttgcgcg gcagcgcgtc caccacgcgc acctcgcggg gccgcttgtg tatcgaaagt tgttgggcga catggtcgat caggtcgtcg gggttgaccc ggccggcggg aacgacgaac gcgacgatgc gctgccccag gtcctcgtcg ggcatcccga cgacggcggc ctcggcgacg ccggggtggc cgagcagcgc ggtttcnatt tcgccggcac cgatgcggta gccgccggac ttgatcagat cgacggactc ccgcccgacg atgcggtgca tgccggcgtc gtcgacgacg gcgacntcgc cggtgcggta ccagccgtcg gcgtcnaagg cctcggcggt ggcgtcgggc cggttgaggt nncntcgaac atcnncnggc cncgnaccan cagcttgcnn acngnctcnn ncgngnngnn ccgggttgcc gncntcgtcg accacnnggg nctgnnngnn ggnnnnngnn nggnccnnnn agccgngnnn ncnntngnnn ncngnncggn ngganatggt gatnanngan nccgannagc cgnnnnngtn nanggnnnnn nnnngng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLTSLNP SAVTATDIPD AVRIDGTVLS RADLLGAATS VAERVAGAGR VAVLATPTAA TVLAVTGCLI AGVPFVPVPA DVGAAERRHM LADSGVRAWL GPLPDEPDGL PHVPVRLHAR SWHRYPEPSP DATAMIIYTS GTTGLPKGVV LSRRAIAADL DALAQAWQWT ADDVLVHGLP LFHVHGLVLG LLGSLRIGNR FVHTGKPTPA GYAQARTDFG GTLFFGVPTV WSRVVADEAA ARALRPARLL VSGSAPLPVP VFDRLAGLTG HQPVERYGAS ESLITISTRA DGERRPGWVG LPLTGVQTRV VDDDGNPVPH DGETVGKLLV RGPMMFDGYL NRPDATAEAF DADGWYRTGD VAVVDDAGMH RIVGRESVDL IKSGGYRIGA GEIETALLGH PGVAEAAVVG MPDEDLGQRI VAFVVPAGRV NPDDLIDHVA QQLSIHKRPR EVRVVDALPR NAMGKVLKKQ LLSDG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTGCTG ACGTCGCTGA ATCCCTCGGC CGTTACCGCC ACCGACATCC CCGACGCGGT ACGCATCGAC GGCACCGTGC TCAGCCGCGC CGACCTGCTC GGCGCCGCGA CCTCGGTGGC CGAGCGGGTC GCCGGTGCGG GCCGGGTCGC CGTCCTCGCC ACACCGACCG CCGCCACGGT GCTCGCGGTG ACCGGGTGCC TGATCGCCGG GGTGCCGTTC GTCCCGGTGC CCGCCGACGT CGGGGCCGCC GAACGCCGGC ACATGCTGGC CGACTCCGGG GTGCGGGCCT GGCTCGGCCC CCTGCCTGAC GAGCCCGACG GCCTGCCGCA CGTCCCGGTG CGGCTGCACG CCCGCTCCTG GCACCGCTAC CCCGAGCCGT CACCGGACGC CACCGCGATG ATCATCTACA CCTCGGGCAC CACCGGGCTG CCCAAGGGCG TGGTGCTGAG CCGGCGCGCG ATCGCCGCCG ACCTGGACGC CCTCGCGCAG GCCTGGCAGT GGACGGCCGA CGACGTGCTG GTGCACGGGC TGCCGCTGTT TCACGTGCAC GGTCTGGTGC TGGGCCTGCT GGGCTCGCTG CGGATCGGGA ATCGGTTCGT CCACACCGGA AAACCGACCC CGGCCGGCTA CGCGCAGGCC CGCACCGACT TCGGCGGCAC CCTGTTCTTC GGGGTGCCGA CGGTGTGGTC GCGGGTTGTG GCCGACGAGG CGGCCGCCCG CGCGCTGCGC CCGGCGCGGC TGCTGGTCTC CGGCAGCGCG CCGCTGCCGG TCCCGGTGTT CGACCGGTTG GCCGGGCTCA CCGGGCACCA GCCCGTCGAA CGCTACGGCG CCTCGGAATC GCTGATCACC ATCTCCACCC GGGCCGACGG CGAGCGGCGC CCCGGCTGGG TGGGCCTGCC GCTGACCGGC GTGCAGACCC GGGTGGTCGA CGACGACGGC AACCCGGTGC CGCACGACGG GGAGACCGTC GGCAAGCTGC TGGTGCGCGG CCCGATGATG TTCGACGGCT ACCTCAACCG GCCCGACGCC ACCGCCGAGG CCTTCGACGC CGACGGCTGG TACCGCACCG GCGACGTCGC CGTCGTCGAC GACGCCGGCA TGCACCGCAT CGTCGGGCGG GAGTCCGTCG ATCTGATCAA GTCCGGCGGC TACCGCATCG GTGCCGGCGA AATCGAAACC GCGCTGCTCG GCCACCCCGG CGTCGCCGAG GCCGCCGTCG TCGGGATGCC CGACGAGGAC CTGGGGCAGC GCATCGTCGC GTTCGTCGTT CCCGCCGGCC GGGTCAACCC CGACGACCTG ATCGACCATG TCGCCCAACA ACTTTCGATA CACAAGCGGC CCCGCGAGGT GCGCGTGGTG GACGCGCTGC CGCGCAACGC CATGGGCAAG GTGCTCAAGA AGCAGCTGCT GTCCGACGGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA