MymaA.00629.a

Fatty-acid-CoA synthetase, FadD36

CENTER ID: MymaA.00629.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00629.a.A1.GE30051 Full length( MymaA.00629.a ) 1 472
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4556
RefSeq: YP_001852506.1
UniProt: B2HS73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLASLNPAA VSADDIADAV CIDGVDLSRS DLLGAATAVA ERIAGAHRVA VLATPTVSTV LAVTGCLIAG VPVVPVPADV GVAERRHMLA DSGAVAWLGP APAQDAEPDG LPQIPVRLHA RSWHRYPEPP PDSVAMVIYT SGTTGLPKGV QLSRRAIAAD LDALAQAWQW NAEDVLVHGL PLYHVHGLVL GLLGSLRVGN RFVHTGKPTA AGYAQAGGTL YFGVPTVWSR VAADRAAAEA LRPARLLVSG SAALPVPVFD QLVHLAGHRP IERYGSSESL ITLSTRADGE RRAGWVGLPL AGVQTRLVDD HGNPVPHDGE TVGKLQVRGS TLFDGYLNRP EATAEAFDAD GWYRTGDVAV VDDGGMHRIV GRESVDLIKS GGYRIGAGEI ETVLLGHPEV QEAAVVGLPD EDLGQRIVAY IVGSDRIDPD VLIDFVAQQL SVHKRPREVR IVESLPRNAL GKVLKKQLLS EG
NT Sequence
GTGCTGCTGG CCTCACTGAA CCCCGCCGCC GTATCGGCTG ACGACATCGC CGACGCGGTT TGTATCGACG GCGTCGACCT CAGCCGCAGC GATCTGCTCG GCGCCGCAAC GGCGGTGGCG GAGCGTATCG CTGGCGCGCA TCGGGTTGCC GTACTGGCAA CGCCCACCGT ATCGACGGTG CTGGCGGTCA CCGGATGCTT GATCGCCGGG GTGCCCGTGG TCCCGGTGCC GGCCGATGTC GGTGTGGCCG AACGCCGCCA CATGCTGGCC GACTCCGGCG CCGTGGCCTG GCTGGGCCCG GCGCCCGCCC AGGACGCGGA GCCCGACGGA CTGCCGCAGA TACCGGTGCG TCTGCACGCA CGATCATGGC ACCGCTACCC CGAGCCGCCG CCGGACTCGG TTGCGATGGT GATCTACACC TCGGGCACCA CGGGGCTGCC CAAGGGCGTG CAGCTGAGCC GGCGGGCGAT TGCAGCCGAC TTGGACGCGC TGGCCCAGGC CTGGCAATGG AATGCAGAAG ACGTCTTGGT GCATGGCTTG CCGCTCTACC ATGTGCACGG TCTGGTACTC GGCTTGCTGG GATCGCTGCG AGTTGGAAAT CGCTTCGTGC ACACCGGAAA GCCGACGGCG GCGGGATACG CGCAAGCAGG CGGAACCCTG TATTTCGGGG TGCCCACGGT CTGGTCGCGG GTGGCTGCGG ACCGGGCGGC CGCCGAGGCG CTACGGCCGG CTCGGCTATT GGTTTCTGGC AGCGCGGCCT TGCCGGTCCC GGTGTTCGAT CAGCTGGTGC ACCTCGCCGG GCACCGCCCT ATCGAGCGCT ATGGCTCTTC GGAATCGCTG ATCACCCTGT CTACCCGGGC TGACGGCGAG CGCCGCGCGG GCTGGGTGGG CCTGCCGCTG GCCGGAGTGC AGACCCGGCT GGTCGATGAT CACGGCAACC CGGTCCCGCA TGACGGGGAA ACCGTTGGGA AGCTGCAGGT TCGGGGCTCG ACACTGTTCG ATGGCTACCT GAATCGGCCT GAGGCCACGG CTGAGGCGTT CGATGCCGAC GGCTGGTATC GCACCGGCGA CGTTGCGGTC GTTGACGACG GTGGCATGCA TCGCATCGTT GGCCGTGAGT CGGTTGATTT GATCAAGTCT GGTGGCTACC GTATCGGCGC CGGGGAGATC GAGACGGTGC TACTGGGGCA TCCGGAGGTT CAAGAGGCAG CTGTCGTCGG ATTGCCTGAC GAGGATTTGG GCCAGCGGAT CGTGGCCTAC ATCGTGGGTT CGGACCGTAT CGATCCCGAC GTATTGATCG ACTTTGTCGC GCAACAGCTT TCGGTCCACA AACGGCCACG CGAGGTGCGG ATTGTGGAGT CGTTGCCGCG CAACGCGCTG GGCAAGGTGC TCAAGAAGCA GCTGTTGTCA GAGGGC
Details for MymaA.00629.a.A1.GE30051
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 72
PERCENT COVERAGE: 41
Validated AA Sequence
MAHHHHHHMG TLEAXTQGPG SMVLLASLNP AAVSADDIAD AVCIDGVDLS RSDLLGAATA VAERIAGAHR VAVLATPTVS TVLAVTGCLI AGVPVVPVPA DVGVAERRXM LADSGAVAXX XPAPXQXXXP DGLPQXPVRL HXXSWXRYXX XXPXSXAXVX YTXDHXXAQX XAXEPXXDCX XXGXXXXAWX XXEXXXXXXX XRSTXX
Validated NT Sequence
tnnnnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc anacccaggg tcctggttcg atggtgctgc tggcctcact gaaccccgcc gccgtatcgg ctgacgacat cgccgacgcg gtttgtatcg acggcgtcga cctcagccgc agcgatctgc tcggcgccgc aacggcggtg gcggagcgta tcgctggcgc gcatcgggtt gccgtactgg caacgcccac cgtatcgacg gtgctggcgg tcaccggatg cttgatcgcc ggggtgcccg tggtcccggt gccggccgat gtcggtgtgg ccgaacgccg ccncatgctg gccgactccg gcgccgtggc ctgnnnggnc ccggcgcccg nccaggannn gnagcccgac ggactgccgc agatnccggt gcgtctgcac nnacnatcat ggnnccgcta ccnnnagncg ncgccggant cnnttgcnat ngtganctac acctnngacc acgngnntgc ccaangnngn gcangtgagc cgncggnnga ttgcngcnna nttggacncn ctngnnncgc ctggnaatgn nnngaanann annnntgnng nanggnnngc cgctcnacnn nnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLASLNP AAVSADDIAD AVCIDGVDLS RSDLLGAATA VAERIAGAHR VAVLATPTVS TVLAVTGCLI AGVPVVPVPA DVGVAERRHM LADSGAVAWL GPAPAQDAEP DGLPQIPVRL HARSWHRYPE PPPDSVAMVI YTSGTTGLPK GVQLSRRAIA ADLDALAQAW QWNAEDVLVH GLPLYHVHGL VLGLLGSLRV GNRFVHTGKP TAAGYAQAGG TLYFGVPTVW SRVAADRAAA EALRPARLLV SGSAALPVPV FDQLVHLAGH RPIERYGSSE SLITLSTRAD GERRAGWVGL PLAGVQTRLV DDHGNPVPHD GETVGKLQVR GSTLFDGYLN RPEATAEAFD ADGWYRTGDV AVVDDGGMHR IVGRESVDLI KSGGYRIGAG EIETVLLGHP EVQEAAVVGL PDEDLGQRIV AYIVGSDRID PDVLIDFVAQ QLSVHKRPRE VRIVESLPRN ALGKVLKKQL LSE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgctg gcctcactga accccgccgc cgtatcggct gacgacatcg ccgacgcggt ttgtatcgac ggcgtcgacc tcagccgcag cgatctgctc ggcgccgcaa cggcggtggc ggagcgtatc gctggcgcgc atcgggttgc cgtactggca acgcccaccg tatcgacggt gctggcggtc accggatgct tgatcgccgg ggtgcccgtg gtcccggtgc cggccgatgt cggtgtggcc gaacgccgcc acatgctggc cgactccggc gccgtggcct ggctgggccc ggcgcccgcc caggacgcgg agcccgacgg actgccgcag ataccggtgc gtctgcacgc acgatcatgg caccgctacc ccgagccgcc gccggactcg gttgcgatgg tgatctacac ctcgggcacc acggggctgc ccaagggcgt gcagctgagc cggcgggcga ttgcagccga cttggacgcg ctggcccagg cctggcaatg gaatgcagaa gacgtcttgg tgcatggctt gccgctctac catgtgcacg gtctggtact cggcttgctg ggatcgctgc gagttggaaa tcgcttcgtg cacaccggaa agccgacggc ggcgggatac gcgcaagcag gcggaaccct gtatttcggg gtgcccacgg tctggtcgcg ggtggctgcg gaccgggcgg ccgccgaggc gctacggccg gctcggctat tggtttctgg cagcgcggcc ttgccggtcc cggtgttcga tcagctggtg cacctcgccg ggcaccgccc tatcgagcgc tatggctctt cggaatcgct gatcaccctg tctacccggg ctgacggcga gcgccgcgcg ggctgggtgg gcctgccgct ggccggagtg cagacccggc tggtcgatga tcacggcaac ccggtcccgc atgacgggga aaccgttggg aagctgcagg ttcggggctc gacactgttc gatggctacc tgaatcggcc tgaggccacg gctgaggcgt tcgatgccga cggctggtat cgcaccggcg acgttgcggt cgttgacgac ggtggcatgc atcgcatcgt tggccgtgag tcggttgatt tgatcaagtc tggtggctac cgtatcggcg ccggggagat cgagacggtg ctactggggc atccggaggt tcaagaggca gctgtcgtcg gattgcctga cgaggatttg ggccagcgga tcgtggccta catcgtgggt tcggaccgta tcgatcccga cgtattgatc gactttgtcg cgcaacagct ttcggtccac aaacggccac gcgaggtgcg gattgtggag tcgttgccgc gcaacgcgct gggcaaggtg ctcaagaagc agctgttgtc agagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa