MytuD.00386.a

Enoyl-CoA hydratase

CENTER ID: MytuD.00386.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00386.a.A1.GE25833 Full length( MytuD.00386.a ) 1 263
MytuD.00386.a.AE1.GE43169 Full length( MytuD.00386.a ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00386.a.AE1.PW38675 Full length( MytuD.00386.a ) 1 263

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_133017
PATRIC ID: fig|83332.12.peg.752
RefSeq: YP_177745.1
TubercuList: Rv0675
MTB Network Portal: Rv0675
UniProt: I6Y4E8

Orthologues for Rv0675

Species % Identity % Coverage Clones Proteins Structures
marinum 90 97 1 1 1
ulcerans 89 97 1 1 0
paratuberculosis 87 97 1 1 0
smegmatis 82 97 1 1 1
abscessus 76 96 1 1 1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA RRFAAGAGRH GAPAPRAEQG DTL
NT Sequence
ATGAGTGATC TGGTGCGTGT GGAGCGCAAA GGTCGGGTGA CCACGGTGAT TCTGAACCGG CCGGCCTCCC GCAACGCGGT CAACGGCCCG ACCGCCGCGG CGTTGTGCGC GGCGTTCGAG CAATTCGACC GGGACGACGC CGCGTCGGTG GCCGTACTCT GGGGTGCGGG TGGAACCTTT TGTGCGGGAG CCGATTTGAA GGCCTTTGGC ACACCGGAGG CCAACTCTGT GCACCGGACG GGTCCCGGCC CGATGGGGCC GTCACGAATG ATGCTGTCCA AACCTGTGAT CGCCGCCGTC AGCGGCTACG CCGTCGCCGG GGGGCTGGAA TTGGCACTGT GGTGCGACCT GCGGGTGGCC GAGGAAGACG CCGTGTTCGG TGTGTTTTGC CGTCGCTGGG GGGTACCGCT CATCGACGGC GGCACCGTGC GACTGCCACG GCTGATCGGG CACAGCCGCG CGATGGACAT GATCCTCACT GGCCGTGGGG TGCCGGCCGA CGAAGCGCTG GCCATGGGGT TGGCCAATCG GGTGGTGCCC AAGGGTCAAG CCCGACAGGC GGCTGAGGAG TTGGCGGCGC AATTGGCCGC GCTGCCGCAG CAGTGTCTGC GATCGGATCG GCTGTCGGCG CTGCACCAGT GGGGCCTGCC CGAGTCCGCG GCGCTCGACC TCGAGTTCGC CAGCATCGCG CGGGTGGCCG GCGAGGCGCT AGAGGGGGCG AGACGGTTCG CCGCGGGTGC CGGTCGGCAT GGGGCCCCGG CACCTCGGGC CGAACAGGGC GACACGCTT
Details for MytuD.00386.a.A1.GE25833
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLVRVER KGRVTTVILN RPASRNAVNG PTAAALCAAF EQFDRDDAAS VAVLWGAGGT FCAGADLKAF GTPEANSVHR TGPGPMGPSR MMLSKPVIAA VSGYAVAGGL ELALWCDLRV AEEDAVFGVF CRRWGVPLID GGTVRLPRLI GHSRAMDMIL TGRGVPADEA LAMGLANRVV PKGQARQAAE ELAAQLAALP QQCLRSDRLS ALHQWGLPES AALDLEFASI ARVAGEALEG ARRFAAGAGR HGAPAPRAEQ GDTL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atctggtgcg tgtggagcgc aaaggtcggg tgaccacggt gattctgaac cggccggcct cccgcaacgc ggtcaacggc ccgaccgccg cggcgttgtg cgcggcgttc gagcaattcg accgggacga cgccgcgtcg gtggccgtac tctggggtgc gggtggaacc ttttgtgcgg gagccgattt gaaggccttt ggcacaccgg aggccaactc tgtgcaccgg acgggtcccg gcccgatggg gccgtcacga atgatgctgt ccaaacctgt gatcgccgcc gtcagcggct acgccgtcgc cggggggctg gaattggcac tgtggtgcga cctgcgggtg gccgaggaag acgccgtgtt cggtgtgttt tgccgtcgct ggggggtacc gctcatcgac ggcggcaccg tgcgactgcc acggctgatc gggcacagcc gcgcgatgga catgatcctc actggccgtg gggtgccggc cgacgaagcg ctggccatgg ggttggccaa tcgggtggtg cccaagggtc aagcccgaca ggcggctgag gagttggcgg cgcaattggc cgcgctgccg cagcagtgtc tgcgatcgga tcggctgtcg gcgctgcacc agtggggcct gcccgagtcc gcggcgctcg acctcgagtt cgccagcatc gcgcgggtgg ccggcgaggc gctagagggg gcgagacggt tcgccgcggg tgccggtcgg catggggccc cggcacctcg ggccgaacag ggcgacacgc tttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDLVRVER KGRVTTVILN RPASRNAVNG PTAAALCAAF EQFDRDDAAS VAVLWGAGGT FCAGADLKAF GTPEANSVHR TGPGPMGPSR MMLSKPVIAA VSGYAVAGGL ELALWCDLRV AEEDAVFGVF CRRWGVPLID GGTVRLPRLI GHSRAMDMIL TGRGVPADEA LAMGLANRVV PKGQARQAAE ELAAQLAALP QQCLRSDRLS ALHQWGLPES AALDLEFASI ARVAGEALEG ARRFAAGAGR HGAPAPRAEQ GDTL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgatctg gtgcgtgtgg agcgcaaagg tcgggtgacc acggtgattc tgaaccggcc ggcctcccgc aacgcggtca acggcccgac cgccgcggcg ttgtgcgcgg cgttcgagca attcgaccgg gacgacgccg cgtcggtggc cgtactctgg ggtgcgggtg gaaccttttg tgcgggagcc gatttgaagg cctttggcac accggaggcc aactctgtgc accggacggg tcccggcccg atggggccgt cacgaatgat gctgtccaaa cctgtgatcg ccgccgtcag cggctacgcc gtcgccgggg ggctggaatt ggcactgtgg tgcgacctgc gggtggccga ggaagacgcc gtgttcggtg tgttttgccg tcgctggggg gtaccgctca tcgacggcgg caccgtgcga ctgccacggc tgatcgggca cagccgcgcg atggacatga tcctcactgg ccgtggggtg ccggccgacg aagcgctggc catggggttg gccaatcggg tggtgcccaa gggtcaagcc cgacaggcgg ctgaggagtt ggcggcgcaa ttggccgcgc tgccgcagca gtgtctgcga tcggatcggc tgtcggcgct gcaccagtgg ggcctgcccg agtccgcggc gctcgacctc gagttcgcca gcatcgcgcg ggtggccggc gaggcgctag agggggcgag acggttcgcc gcgggtgccg gtcggcatgg ggccccggca cctcgggccg aacagggcga cacgcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.00386.a.AE1.GE43169
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA RRFAAGAGRH GAPAPRAEQG DTLAGHHHHH H
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag caagcgtgtc gccctgttcg gcccgaggtg ccggggcccc atgccgaccg gcacccgcgg cgaaccgtct cgccccctct agcgcctcgc cggccacccg cgcgatgctg gcgaactcga ggtcgagcgc cgcggactcg ggcaggcccc actggtgcag cgccgacagc cgatccgatc gcagacactg ctgcggcagc gcggccaatt gcgccgccaa ctcctcagcc gcctgtcggg cttgaccctt gggcaccacc cgattggcca accccatggc cagcgcttcg tcggccggca ccccacggcc agtgaggatc atgtccatcg cgcggctgtg cccgatcagc cgtggcagtc gcacggtgcc gccgtcgatg agcggtaccc cccagcgacg gcaaaacaca ccgaacacgg cgtcttcctc ggccacccgc aggtcgcacc acagtgccaa ttccagcccc ccggcgacgg cgtagccgct gacggcggcg atcacaggtt tggacagcat cattcgtgac ggccccatcg ggccgggacc cgtccggtgc acagagttgg cctccggtgt gccaaaggcc ttcaaatcgg ctcccgcaca aaaggttcca cccgcacccc agagtacggc caccgacgcg gcgtcgtccc ggtcgaattg ctcgaacgcc gcgcacaacg ccgcggcggt cgggccgttg accgcgttgc gggaggccgg ccggttcaga atcaccgtgg tcacccgacc tttgcgctcc acacgcacca gatcactcat gggagaga
Expected Protein Sequence
MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA RRFAAGAGRH GAPAPRAEQG DTLGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gagtgatctg gtgcgtgtgg agcgcaaagg tcgggtgacc acggtgattc tgaaccggcc ggcctcccgc aacgcggtca acggcccgac cgccgcggcg ttgtgcgcgg cgttcgagca attcgaccgg gacgacgccg cgtcggtggc cgtactctgg ggtgcgggtg gaaccttttg tgcgggagcc gatttgaagg cctttggcac accggaggcc aactctgtgc accggacggg tcccggcccg atggggccgt cacgaatgat gctgtccaaa cctgtgatcg ccgccgtcag cggctacgcc gtcgccgggg ggctggaatt ggcactgtgg tgcgacctgc gggtggccga ggaagacgcc gtgttcggtg tgttttgccg tcgctggggg gtaccgctca tcgacggcgg caccgtgcga ctgccacggc tgatcgggca cagccgcgcg atggacatga tcctcactgg ccgtggggtg ccggccgacg aagcgctggc catggggttg gccaatcggg tggtgcccaa gggtcaagcc cgacaggcgg ctgaggagtt ggcggcgcaa ttggccgcgc tgccgcagca gtgtctgcga tcggatcggc tgtcggcgct gcaccagtgg ggcctgcccg agtccgcggc gctcgacctc gagttcgcca gcatcgcgcg ggtggccggc gaggcgctag agggggcgag acggttcgcc gcgggtgccg gtcggcatgg ggccccggca cctcgggccg aacagggcga cacgcttggg caccaccatc atcatcatta acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat
Details for MytuD.00386.a.AE1.PW38675
PURIFICATION DATe: 7/29/2019
CONCENTRATION: 22mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA RRFAAGAGRH GAPAPRAEQG DTLAGHHHHH H
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag caagcgtgtc gccctgttcg gcccgaggtg ccggggcccc atgccgaccg gcacccgcgg cgaaccgtct cgccccctct agcgcctcgc cggccacccg cgcgatgctg gcgaactcga ggtcgagcgc cgcggactcg ggcaggcccc actggtgcag cgccgacagc cgatccgatc gcagacactg ctgcggcagc gcggccaatt gcgccgccaa ctcctcagcc gcctgtcggg cttgaccctt gggcaccacc cgattggcca accccatggc cagcgcttcg tcggccggca ccccacggcc agtgaggatc atgtccatcg cgcggctgtg cccgatcagc cgtggcagtc gcacggtgcc gccgtcgatg agcggtaccc cccagcgacg gcaaaacaca ccgaacacgg cgtcttcctc ggccacccgc aggtcgcacc acagtgccaa ttccagcccc ccggcgacgg cgtagccgct gacggcggcg atcacaggtt tggacagcat cattcgtgac ggccccatcg ggccgggacc cgtccggtgc acagagttgg cctccggtgt gccaaaggcc ttcaaatcgg ctcccgcaca aaaggttcca cccgcacccc agagtacggc caccgacgcg gcgtcgtccc ggtcgaattg ctcgaacgcc gcgcacaacg ccgcggcggt cgggccgttg accgcgttgc gggaggccgg ccggttcaga atcaccgtgg tcacccgacc tttgcgctcc acacgcacca gatcactcat gggagaga
Expressed Protein Sequence
MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA RRFAAGAGRH GAPAPRAEQG DTLGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GAGTGATCTG GTGCGTGTGG AGCGCAAAGG TCGGGTGACC ACGGTGATTC TGAACCGGCC GGCCTCCCGC AACGCGGTCA ACGGCCCGAC CGCCGCGGCG TTGTGCGCGG CGTTCGAGCA ATTCGACCGG GACGACGCCG CGTCGGTGGC CGTACTCTGG GGTGCGGGTG GAACCTTTTG TGCGGGAGCC GATTTGAAGG CCTTTGGCAC ACCGGAGGCC AACTCTGTGC ACCGGACGGG TCCCGGCCCG ATGGGGCCGT CACGAATGAT GCTGTCCAAA CCTGTGATCG CCGCCGTCAG CGGCTACGCC GTCGCCGGGG GGCTGGAATT GGCACTGTGG TGCGACCTGC GGGTGGCCGA GGAAGACGCC GTGTTCGGTG TGTTTTGCCG TCGCTGGGGG GTACCGCTCA TCGACGGCGG CACCGTGCGA CTGCCACGGC TGATCGGGCA CAGCCGCGCG ATGGACATGA TCCTCACTGG CCGTGGGGTG CCGGCCGACG AAGCGCTGGC CATGGGGTTG GCCAATCGGG TGGTGCCCAA GGGTCAAGCC CGACAGGCGG CTGAGGAGTT GGCGGCGCAA TTGGCCGCGC TGCCGCAGCA GTGTCTGCGA TCGGATCGGC TGTCGGCGCT GCACCAGTGG GGCCTGCCCG AGTCCGCGGC GCTCGACCTC GAGTTCGCCA GCATCGCGCG GGTGGCCGGC GAGGCGCTAG AGGGGGCGAG ACGGTTCGCC GCGGGTGCCG GTCGGCATGG GGCCCCGGCA CCTCGGGCCG AACAGGGCGA CACGCTTGGG CACCACCATC ATCATCATTA ACGGATCCGA ATTCGAGCTC CGTCGACAAG CTTGCGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGAT