MysmA.00386.a
Enoyl-CoA hydratase
| CENTER ID: | MysmA.00386.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00386.a.A1.GE28628 | Full length( MysmA.00386.a ) | 1 | 254 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00386.a.A1.PS00557 | Full length( MysmA.00386.a ) | 1 | 254 |
Structures
4QFE
DEPOSITED: 5/20/2014
DETERMINATION: XRay
CLONE: MysmA.00386.a.A1.GE28628
PROTEIN: MysmA.00386.a.A1.PS00557
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133017 |
| PATRIC ID: | fig|246196.19.peg.1373 |
| RefSeq: | YP_885776.1 |
| UniProt: | A0QS88 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEPVRIERN GPVTTVIIDR PEARNAVNGP TAAALFAAFE EFDADDTASV AVLTGANGTF CAGADLKAFG TPEANQVHRE GPGPMGPSRM DLSKPVIAAI SGYAVAGGLE LALWCDLRVV DEDATMGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDLILT GRAVDAAEAY AIGLANRVVP TGQARQAAEE LAADLARLPQ QCMRADRLSA LHQWGESENA AMDFEFASIS RVKDEAMHGA GRFAAGAGRH GANA
NT Sequence
GTGAGCGAAC CCGTCCGGAT CGAACGCAAC GGCCCGGTCA CCACGGTCAT CATCGACCGA CCCGAGGCGA GGAACGCGGT GAACGGCCCG ACCGCCGCGG CGCTGTTCGC CGCGTTCGAG GAGTTCGACG CCGACGACAC CGCGTCGGTG GCGGTGTTGA CCGGCGCGAA CGGAACATTC TGCGCGGGAG CCGATCTGAA GGCGTTCGGT ACGCCCGAGG CGAACCAGGT GCACCGCGAA GGTCCCGGGC CGATGGGCCC GAGCCGGATG GACCTGTCGA AACCGGTGAT CGCGGCGATC AGCGGGTACG CCGTCGCAGG TGGCCTCGAA CTTGCGCTGT GGTGTGACCT GCGCGTGGTC GACGAGGACG CCACGATGGG TGTGTTCTGC CGGCGCTGGG GCGTACCGCT CATCGACGGC GGGACAGTGC GCCTACCGCG GCTGATCGGG CACAGCCGCG CGATGGACCT GATCCTCACC GGGCGCGCGG TCGACGCCGC CGAGGCGTAT GCGATAGGGC TGGCCAACCG TGTGGTCCCC ACCGGGCAGG CCCGCCAGGC GGCCGAGGAA CTCGCCGCCG ACCTGGCGCG TCTGCCCCAG CAGTGCATGC GGGCCGACCG CCTGTCGGCT CTGCACCAGT GGGGCGAATC AGAGAACGCG GCAATGGATT TCGAGTTCGC CTCCATATCG CGGGTCAAGG ACGAGGCCAT GCACGGGGCG GGACGGTTCG CCGCGGGCGC AGGCCGCCAC GGAGCGAACG CG
Details for MysmA.00386.a.A1.GE28628
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEPVRIE RNGPVTTVII DRPEARNAVN GPTAAALFAA FEEFDADDTA SVAVLTGANG TFCAGADLKA FGTPEANQVH REGPGPMGPS RMDLSKPVIA AISGYAVAGG LELALWCDLR VVDEDATMGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDLI LTGRAVDAAE AYAIGLANRV VPTGQARQAA EELAADLARL PQQCMRADRL SALHQWGESE NAAMDFEFAS XSRVKDEAXH GAGXFAAGAG RHGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgaacccgt ccggatcgaa cgcaacggcc cggtcaccac ggtcatcatc gaccgacccg aggcgaggaa cgcggtgaac ggcccgaccg ccgcggcgct gttcgccgcg ttcgaggagt tcgacgccga cgacaccgcg tcggtggcgg tgttgaccgg cgcgaacgga acattctgcg cgggagccga tctgaaggcg ttcggtacgc ccgaggcgaa ccaggtgcac cgcgaaggtc ccgggccgat gggcccgagc cggatggacc tgtcgaaacc ggtgatcgcg gcgatcagcg ggtacgccgt cgcaggtggc ctcgaacttg cgctgtggtg tgacctgcgc gtggtcgacg aggacgccac gatgggtgtg ttctgccggc gctggggcgt accgctcatc gacggcggga cagtgcgcct accgcggctg atcgggcaca gccgcgcgat ggacctgatc ctcaccgggc gcgcggtcga cgccgccgag gcgtatgcga tagggctggc caaccgtgtg gtccccaccg ggcaggcccg ccaggcggcc gaggaactcg ccgccgacct ggcgcgtctg ccccagcagt gcatgcgggc cgaccgcctg tcggctctgc accagtgggg cgaatcagag aacgcggcaa tggatttcga gttcgcctcc ntatcgcggg tcaaggacga ggccntgcac ggggcgggac ngttcgccgc gggcgcnggc cgccacggag cga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEPVRIE RNGPVTTVII DRPEARNAVN GPTAAALFAA FEEFDADDTA SVAVLTGANG TFCAGADLKA FGTPEANQVH REGPGPMGPS RMDLSKPVIA AISGYAVAGG LELALWCDLR VVDEDATMGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDLI LTGRAVDAAE AYAIGLANRV VPTGQARQAA EELAADLARL PQQCMRADRL SALHQWGESE NAAMDFEFAS ISRVKDEAMH GAGRFAAGAG RHGANA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgaa cccgtccgga tcgaacgcaa cggcccggtc accacggtca tcatcgaccg acccgaggcg aggaacgcgg tgaacggccc gaccgccgcg gcgctgttcg ccgcgttcga ggagttcgac gccgacgaca ccgcgtcggt ggcggtgttg accggcgcga acggaacatt ctgcgcggga gccgatctga aggcgttcgg tacgcccgag gcgaaccagg tgcaccgcga aggtcccggg ccgatgggcc cgagccggat ggacctgtcg aaaccggtga tcgcggcgat cagcgggtac gccgtcgcag gtggcctcga acttgcgctg tggtgtgacc tgcgcgtggt cgacgaggac gccacgatgg gtgtgttctg ccggcgctgg ggcgtaccgc tcatcgacgg cgggacagtg cgcctaccgc ggctgatcgg gcacagccgc gcgatggacc tgatcctcac cgggcgcgcg gtcgacgccg ccgaggcgta tgcgataggg ctggccaacc gtgtggtccc caccgggcag gcccgccagg cggccgagga actcgccgcc gacctggcgc gtctgcccca gcagtgcatg cgggccgacc gcctgtcggc tctgcaccag tggggcgaat cagagaacgc ggcaatggat ttcgagttcg cctccatatc gcgggtcaag gacgaggcca tgcacggggc gggacggttc gccgcgggcg caggccgcca cggagcgaac gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00386.a.A1.PS00557
| PURIFICATION DATe: | 4/9/2010 |
| CONCENTRATION: | 60.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEPVRIE RNGPVTTVII DRPEARNAVN GPTAAALFAA FEEFDADDTA SVAVLTGANG TFCAGADLKA FGTPEANQVH REGPGPMGPS RMDLSKPVIA AISGYAVAGG LELALWCDLR VVDEDATMGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDLI LTGRAVDAAE AYAIGLANRV VPTGQARQAA EELAADLARL PQQCMRADRL SALHQWGESE NAAMDFEFAS XSRVKDEAXH GAGXFAAGAG RHGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgaacccgt ccggatcgaa cgcaacggcc cggtcaccac ggtcatcatc gaccgacccg aggcgaggaa cgcggtgaac ggcccgaccg ccgcggcgct gttcgccgcg ttcgaggagt tcgacgccga cgacaccgcg tcggtggcgg tgttgaccgg cgcgaacgga acattctgcg cgggagccga tctgaaggcg ttcggtacgc ccgaggcgaa ccaggtgcac cgcgaaggtc ccgggccgat gggcccgagc cggatggacc tgtcgaaacc ggtgatcgcg gcgatcagcg ggtacgccgt cgcaggtggc ctcgaacttg cgctgtggtg tgacctgcgc gtggtcgacg aggacgccac gatgggtgtg ttctgccggc gctggggcgt accgctcatc gacggcggga cagtgcgcct accgcggctg atcgggcaca gccgcgcgat ggacctgatc ctcaccgggc gcgcggtcga cgccgccgag gcgtatgcga tagggctggc caaccgtgtg gtccccaccg ggcaggcccg ccaggcggcc gaggaactcg ccgccgacct ggcgcgtctg ccccagcagt gcatgcgggc cgaccgcctg tcggctctgc accagtgggg cgaatcagag aacgcggcaa tggatttcga gttcgcctcc ntatcgcggg tcaaggacga ggccntgcac ggggcgggac ngttcgccgc gggcgcnggc cgccacggag cga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEPVRIE RNGPVTTVII DRPEARNAVN GPTAAALFAA FEEFDADDTA SVAVLTGANG TFCAGADLKA FGTPEANQVH REGPGPMGPS RMDLSKPVIA AISGYAVAGG LELALWCDLR VVDEDATMGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDLI LTGRAVDAAE AYAIGLANRV VPTGQARQAA EELAADLARL PQQCMRADRL SALHQWGESE NAAMDFEFAS ISRVKDEAMH GAGRFAAGAG RHGANA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGAA CCCGTCCGGA TCGAACGCAA CGGCCCGGTC ACCACGGTCA TCATCGACCG ACCCGAGGCG AGGAACGCGG TGAACGGCCC GACCGCCGCG GCGCTGTTCG CCGCGTTCGA GGAGTTCGAC GCCGACGACA CCGCGTCGGT GGCGGTGTTG ACCGGCGCGA ACGGAACATT CTGCGCGGGA GCCGATCTGA AGGCGTTCGG TACGCCCGAG GCGAACCAGG TGCACCGCGA AGGTCCCGGG CCGATGGGCC CGAGCCGGAT GGACCTGTCG AAACCGGTGA TCGCGGCGAT CAGCGGGTAC GCCGTCGCAG GTGGCCTCGA ACTTGCGCTG TGGTGTGACC TGCGCGTGGT CGACGAGGAC GCCACGATGG GTGTGTTCTG CCGGCGCTGG GGCGTACCGC TCATCGACGG CGGGACAGTG CGCCTACCGC GGCTGATCGG GCACAGCCGC GCGATGGACC TGATCCTCAC CGGGCGCGCG GTCGACGCCG CCGAGGCGTA TGCGATAGGG CTGGCCAACC GTGTGGTCCC CACCGGGCAG GCCCGCCAGG CGGCCGAGGA ACTCGCCGCC GACCTGGCGC GTCTGCCCCA GCAGTGCATG CGGGCCGACC GCCTGTCGGC TCTGCACCAG TGGGGCGAAT CAGAGAACGC GGCAATGGAT TTCGAGTTCG CCTCCATATC GCGGGTCAAG GACGAGGCCA TGCACGGGGC GGGACGGTTC GCCGCGGGCG CAGGCCGCCA CGGAGCGAAC GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA