MyabA.00386.a
Probable enoyl-coa hydratase/isomerase
| CENTER ID: | MyabA.00386.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00386.a.A1.GE30295 | Full length( MyabA.00386.a ) | 1 | 258 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00386.a.A1.PS00913 | Full length( MyabA.00386.a ) | 1 | 258 |
Structures
3R9Q
DEPOSITED: 3/25/2011
DETERMINATION: XRay
CLONE: MyabA.00386.a.A1.GE30295
PROTEIN: MyabA.00386.a.A1.PS00913
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133017 |
| PATRIC ID: | fig|36809.5.peg.3928 |
| RefSeq: | YP_001704585.1 |
| UniProt: | B1MGI6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEMQPAVR VEKAGPVTTV ILNRPHARNA VDGPTAAALL AAFTEFDADP EASVAVLWGD NGTFCAGADL KAMGTDRGNE LHPHGPGPMG PSRLRLSKPV IAAISGHAVA GGIELALWCD LRVVEEDAVL GVFCRRWGVP LIDGGTIRLP RLIGHSRAMD LILTGRPVHA NEALDIGLVN RVVARGQARE AAETLAAEIA AFPQQCVRAD RDSAIAQWGM AEEAALDNEF GSIERVATEA LEGAGRFAAG EGRHGAGV
NT Sequence
ATGAGTGAAG AGATGCAGCC GGCGGTCCGG GTGGAGAAGG CGGGCCCGGT GACAACGGTC ATTCTCAATC GGCCGCATGC GCGCAACGCC GTCGACGGGC CGACCGCGGC GGCGCTGCTC GCGGCGTTCA CCGAGTTCGA CGCGGATCCC GAGGCGTCGG TGGCGGTGCT GTGGGGTGAC AACGGAACCT TCTGTGCTGG AGCTGATCTC AAGGCGATGG GGACCGACCG CGGTAACGAA CTGCACCCGC ATGGCCCAGG TCCGATGGGC CCTTCGCGGT TGCGGCTGTC CAAACCGGTG ATCGCGGCGA TATCGGGGCA CGCCGTCGCC GGCGGGATCG AGCTCGCGTT GTGGTGCGAC CTTCGGGTGG TCGAGGAGGA CGCCGTGCTG GGTGTGTTCT GCCGGCGTTG GGGCGTTCCG CTCATCGACG GTGGCACGAT CAGGCTGCCG CGGCTGATCG GGCACTCGCG TGCGATGGAT CTCATCCTGA CCGGGCGTCC CGTCCACGCG AATGAGGCGC TGGACATCGG GCTCGTCAAC CGCGTGGTCG CGCGTGGTCA GGCGCGTGAG GCTGCCGAGA CACTGGCTGC CGAGATCGCT GCCTTCCCGC AACAGTGCGT GCGTGCCGAC CGTGATTCGG CCATCGCGCA GTGGGGGATG GCCGAGGAAG CGGCACTGGA CAACGAGTTT GGCAGCATCG AGCGCGTCGC AACGGAGGCG CTGGAGGGTG CGGGTCGGTT CGCCGCGGGT GAGGGCCGGC ATGGAGCCGG GGTC
Details for MyabA.00386.a.A1.GE30295
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEMQPAV RVEKAGPVTT VILNRPHARN AVDGPTAAAL LAAFTEFDAD PEASVAVLWG DNGTFCAGAD LKAMGTDRGN ELHPHGPGPM GPSRLRLSKP VIAAISGHAV AGGIELALWC DLRVVEEDAV LGVFCRRWGV PLIDGGTIRL PRLIGHSRAM DLILTGRPVH ANEALDIGLV NRVVARGQAR EAAETLAAEI AAFPQQCVRA DRDSAIAQWG MAEEAALDNE FGSIERVATE ALEGAGRFAA GEGRHGAGV
Validated NT Sequence
ttattagacc ccggctccat gccggccctc acccgcggcg aaccgacccg caccctccag cgcctccgtt gcgacgcgct cgatgctgcc aaactcgttg tccagtgccg cttcctcggc catcccccac tgcgcgatgg ccgaatcacg gtcggcacgc acgcactgtt gcgggaaggc agcgatctcg gcagccagtg tctcggcagc ctcacgcgcc tgaccacgcg cgaccacgcg gttgacgagc ccgatgtcca gcgcctcatt cgcgtggacg ggacgcccgg tcaggatgag atccatcgca cgcgagtgcc cgatcagccg cggcagcctg atcgtgccac cgtcgatgag cggaacgccc caacgccggc agaacacacc cagcacggcg tcctcctcga ccacccgaag gtcgcaccac aacgcgagct cgatcccgcc ggcgacggcg tgccccgata tcgccgcgat caccggtttg gacagccgca accgcgaagg gcccatcgga cctgggccat gcgggtgcag ttcgttaccg cggtcggtcc ccatcgcctt gagatcagct ccagcacaga aggttccgtt gtcaccccac agcaccgcca ccgacgcctc gggatccgcg tcgaactcgg tgaacgccgc gagcagcgcc gccgcggtcg gcccgtcgac ggcgttgcgc gcatgcggcc gattgagaat gaccgttgtc accgggcccg ccttctccac ccggaccgcc ggctgcatct cttcactcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEMQPAV RVEKAGPVTT VILNRPHARN AVDGPTAAAL LAAFTEFDAD PEASVAVLWG DNGTFCAGAD LKAMGTDRGN ELHPHGPGPM GPSRLRLSKP VIAAISGHAV AGGIELALWC DLRVVEEDAV LGVFCRRWGV PLIDGGTIRL PRLIGHSRAM DLILTGRPVH ANEALDIGLV NRVVARGQAR EAAETLAAEI AAFPQQCVRA DRDSAIAQWG MAEEAALDNE FGSIERVATE ALEGAGRFAA GEGRHGAGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgaagag atgcagccgg cggtccgggt ggagaaggcg ggcccggtga caacggtcat tctcaatcgg ccgcatgcgc gcaacgccgt cgacgggccg accgcggcgg cgctgctcgc ggcgttcacc gagttcgacg cggatcccga ggcgtcggtg gcggtgctgt ggggtgacaa cggaaccttc tgtgctggag ctgatctcaa ggcgatgggg accgaccgcg gtaacgaact gcacccgcat ggcccaggtc cgatgggccc ttcgcggttg cggctgtcca aaccggtgat cgcggcgata tcggggcacg ccgtcgccgg cgggatcgag ctcgcgttgt ggtgcgacct tcgggtggtc gaggaggacg ccgtgctggg tgtgttctgc cggcgttggg gcgttccgct catcgacggt ggcacgatca ggctgccgcg gctgatcggg cactcgcgtg cgatggatct catcctgacc gggcgtcccg tccacgcgaa tgaggcgctg gacatcgggc tcgtcaaccg cgtggtcgcg cgtggtcagg cgcgtgaggc tgccgagaca ctggctgccg agatcgctgc cttcccgcaa cagtgcgtgc gtgccgaccg tgattcggcc atcgcgcagt gggggatggc cgaggaagcg gcactggaca acgagtttgg cagcatcgag cgcgtcgcaa cggaggcgct ggagggtgcg ggtcggttcg ccgcgggtga gggccggcat ggagccgggg tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.00386.a.A1.PS00913
| PURIFICATION DATe: | 12/22/2010 |
| CONCENTRATION: | 72.23mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEMQPAV RVEKAGPVTT VILNRPHARN AVDGPTAAAL LAAFTEFDAD PEASVAVLWG DNGTFCAGAD LKAMGTDRGN ELHPHGPGPM GPSRLRLSKP VIAAISGHAV AGGIELALWC DLRVVEEDAV LGVFCRRWGV PLIDGGTIRL PRLIGHSRAM DLILTGRPVH ANEALDIGLV NRVVARGQAR EAAETLAAEI AAFPQQCVRA DRDSAIAQWG MAEEAALDNE FGSIERVATE ALEGAGRFAA GEGRHGAGV
Validated NT Sequence
ttattagacc ccggctccat gccggccctc acccgcggcg aaccgacccg caccctccag cgcctccgtt gcgacgcgct cgatgctgcc aaactcgttg tccagtgccg cttcctcggc catcccccac tgcgcgatgg ccgaatcacg gtcggcacgc acgcactgtt gcgggaaggc agcgatctcg gcagccagtg tctcggcagc ctcacgcgcc tgaccacgcg cgaccacgcg gttgacgagc ccgatgtcca gcgcctcatt cgcgtggacg ggacgcccgg tcaggatgag atccatcgca cgcgagtgcc cgatcagccg cggcagcctg atcgtgccac cgtcgatgag cggaacgccc caacgccggc agaacacacc cagcacggcg tcctcctcga ccacccgaag gtcgcaccac aacgcgagct cgatcccgcc ggcgacggcg tgccccgata tcgccgcgat caccggtttg gacagccgca accgcgaagg gcccatcgga cctgggccat gcgggtgcag ttcgttaccg cggtcggtcc ccatcgcctt gagatcagct ccagcacaga aggttccgtt gtcaccccac agcaccgcca ccgacgcctc gggatccgcg tcgaactcgg tgaacgccgc gagcagcgcc gccgcggtcg gcccgtcgac ggcgttgcgc gcatgcggcc gattgagaat gaccgttgtc accgggcccg ccttctccac ccggaccgcc ggctgcatct cttcactcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEMQPAV RVEKAGPVTT VILNRPHARN AVDGPTAAAL LAAFTEFDAD PEASVAVLWG DNGTFCAGAD LKAMGTDRGN ELHPHGPGPM GPSRLRLSKP VIAAISGHAV AGGIELALWC DLRVVEEDAV LGVFCRRWGV PLIDGGTIRL PRLIGHSRAM DLILTGRPVH ANEALDIGLV NRVVARGQAR EAAETLAAEI AAFPQQCVRA DRDSAIAQWG MAEEAALDNE FGSIERVATE ALEGAGRFAA GEGRHGAGV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGAAGAG ATGCAGCCGG CGGTCCGGGT GGAGAAGGCG GGCCCGGTGA CAACGGTCAT TCTCAATCGG CCGCATGCGC GCAACGCCGT CGACGGGCCG ACCGCGGCGG CGCTGCTCGC GGCGTTCACC GAGTTCGACG CGGATCCCGA GGCGTCGGTG GCGGTGCTGT GGGGTGACAA CGGAACCTTC TGTGCTGGAG CTGATCTCAA GGCGATGGGG ACCGACCGCG GTAACGAACT GCACCCGCAT GGCCCAGGTC CGATGGGCCC TTCGCGGTTG CGGCTGTCCA AACCGGTGAT CGCGGCGATA TCGGGGCACG CCGTCGCCGG CGGGATCGAG CTCGCGTTGT GGTGCGACCT TCGGGTGGTC GAGGAGGACG CCGTGCTGGG TGTGTTCTGC CGGCGTTGGG GCGTTCCGCT CATCGACGGT GGCACGATCA GGCTGCCGCG GCTGATCGGG CACTCGCGTG CGATGGATCT CATCCTGACC GGGCGTCCCG TCCACGCGAA TGAGGCGCTG GACATCGGGC TCGTCAACCG CGTGGTCGCG CGTGGTCAGG CGCGTGAGGC TGCCGAGACA CTGGCTGCCG AGATCGCTGC CTTCCCGCAA CAGTGCGTGC GTGCCGACCG TGATTCGGCC ATCGCGCAGT GGGGGATGGC CGAGGAAGCG GCACTGGACA ACGAGTTTGG CAGCATCGAG CGCGTCGCAA CGGAGGCGCT GGAGGGTGCG GGTCGGTTCG CCGCGGGTGA GGGCCGGCAT GGAGCCGGGG TCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA