MymaA.00386.a
Enoyl-CoA hydratase, EchA5
| CENTER ID: | MymaA.00386.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00386.a.A1.GE30016 | Full length( MymaA.00386.a ) | 1 | 257 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00386.a.A1.PS00820 | Full length( MymaA.00386.a ) | 1 | 257 |
Structures
3QKA
DEPOSITED: 1/31/2011
DETERMINATION: XRay
CLONE: MymaA.00386.a.A1.GE30016
PROTEIN: MymaA.00386.a.A1.PS00820
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133017 |
| PATRIC ID: | fig|216594.6.peg.1072 |
| RefSeq: | YP_001849316.1 |
| UniProt: | B2HSK3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDLVQVERN GPVTTVIINR PQARNAVNGP TAAALYSAFA EFDRDESASV AVLCGNGGTF CAGADLKAFG TAEANAVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EQDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRAVQADEAL AIGLANRVVP NGQARQAAEE LAAQLAALPQ QCLRSDRLSA LQQWGLPESA ALDLEFASIS RVAAEALEGA GRFAAGAGRH GAQASHD
NT Sequence
GTGAGTGATC TAGTACAGGT CGAGCGCAAC GGTCCGGTAA CCACCGTGAT CATCAACCGG CCGCAGGCGC GCAACGCGGT CAACGGTCCG ACGGCTGCCG CGCTCTACTC GGCATTCGCC GAATTCGACC GGGATGAATC CGCTTCGGTG GCCGTCTTGT GCGGAAATGG CGGAACCTTC TGCGCGGGAG CGGATTTGAA GGCTTTCGGC ACCGCGGAGG CCAACGCCGT GCACCGGACG GGTCCGGGCC CGATGGGCCC GTCGCGAATG ATGCTGTCCA AGCCGGTGAT CGCCGCGGTC AGTGGCTACG CGGTGGCCGG GGGACTGGAG CTGGCGTTGT GGTGCGATCT GCGGGTGGCC GAACAGGACG CGGTATTCGG CGTGTTCTGT CGGCGGTGGG GCGTGCCGCT CATCGACGGC GGCACGGTGC GGCTGCCGCG GCTGATCGGC CACAGCCGGG CGATGGACAT GATCCTTACC GGTCGTGCGG TGCAGGCCGA CGAGGCGCTG GCAATCGGGT TGGCGAATCG GGTGGTACCC AACGGACAAG CCCGGCAAGC AGCCGAGGAG TTGGCGGCCC AGCTGGCGGC GCTGCCACAA CAATGCCTGC GATCGGATCG CCTCTCAGCG CTTCAGCAAT GGGGCTTGCC CGAATCCGCG GCTCTCGATC TGGAATTCGC CAGCATTTCC CGAGTGGCCG CCGAGGCGTT GGAGGGGGCC GGGCGCTTCG CCGCGGGTGC CGGTCGCCAT GGCGCCCAGG CGAGCCACGA C
Details for MymaA.00386.a.A1.GE30016
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGPS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDAVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASH
Validated NT Sequence
ttattagtgg ctcgcctggg cgccatggcg accggcaccc gcggcgaagc gcccggcccc ctccaacgcc tcggcggcca ctcgggaaat gctggcgaat tccagatcga gagccgcgga ttcgggcaag ccccattgct gaagcgctga gaggcgatcc gatcgcaggc attgttgtgg cagcgccgcc agctgggccg ccaactcctc ggctgcttgc cgggcttgtc cgttgggtac cacccgattc gccaacccga ttgccagcgc ctcgtcggcc tgcaccgcac gaccggtaag gatcatgtcc atcgcccggc tgtggccgat cagccgcggc agccgcaccg tgccgccgtc gatgagcggc acgccccacc gccgacagaa cacgccgaat accgcgtcct gttcggccac ccgcagatcg caccacaacg ccagctccag tcccccggcc accgcgtagc cactgaccgc ggcgatcacc ggcttggaca gcatcattcg cgacgggccc atcgggcccg gacccgtccg gtgcacggcg ttggcctccg cggtgccgaa agccttcaaa tccgctcccg cgcagaaggt tccgccattt ccgcacaaga cggccaccga agcggattca tcccggtcga attcggcgaa tgccgagtag agcgcggcag ccgtcggacc gttgaccgcg ttgcgcgcct gcggccggtt gatgatcacg gtggttaccg gaccgttgcg ctcgacctgt actagatcac tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGPS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDAVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgat ctagtacagg tcgagcgcaa cggtccggta accaccgtga tcatcaaccg gccgcaggcg cgcaacgcgg tcaacggtcc gacggctgcc gcgctctact cggcattcgc cgaattcgac cgggatgaat ccgcttcggt ggccgtcttg tgcggaaatg gcggaacctt ctgcgcggga gcggatttga aggctttcgg caccgcggag gccaacgccg tgcaccggac gggtccgggc ccgatgggcc cgtcgcgaat gatgctgtcc aagccggtga tcgccgcggt cagtggctac gcggtggccg ggggactgga gctggcgttg tggtgcgatc tgcgggtggc cgaacaggac gcggtattcg gcgtgttctg tcggcggtgg ggcgtgccgc tcatcgacgg cggcacggtg cggctgccgc ggctgatcgg ccacagccgg gcgatggaca tgatccttac cggtcgtgcg gtgcaggccg acgaggcgct ggcaatcggg ttggcgaatc gggtggtacc caacggacaa gcccggcaag cagccgagga gttggcggcc cagctggcgg cgctgccaca acaatgcctg cgatcggatc gcctctcagc gcttcagcaa tggggcttgc ccgaatccgc ggctctcgat ctggaattcg ccagcatttc ccgagtggcc gccgaggcgt tggagggggc cgggcgcttc gccgcgggtg ccggtcgcca tggcgcccag gcgagccaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MymaA.00386.a.A1.PS00820
| PURIFICATION DATe: | 10/21/2010 |
| CONCENTRATION: | 46.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGPS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDAVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASH
Validated NT Sequence
ttattagtgg ctcgcctggg cgccatggcg accggcaccc gcggcgaagc gcccggcccc ctccaacgcc tcggcggcca ctcgggaaat gctggcgaat tccagatcga gagccgcgga ttcgggcaag ccccattgct gaagcgctga gaggcgatcc gatcgcaggc attgttgtgg cagcgccgcc agctgggccg ccaactcctc ggctgcttgc cgggcttgtc cgttgggtac cacccgattc gccaacccga ttgccagcgc ctcgtcggcc tgcaccgcac gaccggtaag gatcatgtcc atcgcccggc tgtggccgat cagccgcggc agccgcaccg tgccgccgtc gatgagcggc acgccccacc gccgacagaa cacgccgaat accgcgtcct gttcggccac ccgcagatcg caccacaacg ccagctccag tcccccggcc accgcgtagc cactgaccgc ggcgatcacc ggcttggaca gcatcattcg cgacgggccc atcgggcccg gacccgtccg gtgcacggcg ttggcctccg cggtgccgaa agccttcaaa tccgctcccg cgcagaaggt tccgccattt ccgcacaaga cggccaccga agcggattca tcccggtcga attcggcgaa tgccgagtag agcgcggcag ccgtcggacc gttgaccgcg ttgcgcgcct gcggccggtt gatgatcacg gtggttaccg gaccgttgcg ctcgacctgt actagatcac tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGPS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDAVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTGAT CTAGTACAGG TCGAGCGCAA CGGTCCGGTA ACCACCGTGA TCATCAACCG GCCGCAGGCG CGCAACGCGG TCAACGGTCC GACGGCTGCC GCGCTCTACT CGGCATTCGC CGAATTCGAC CGGGATGAAT CCGCTTCGGT GGCCGTCTTG TGCGGAAATG GCGGAACCTT CTGCGCGGGA GCGGATTTGA AGGCTTTCGG CACCGCGGAG GCCAACGCCG TGCACCGGAC GGGTCCGGGC CCGATGGGCC CGTCGCGAAT GATGCTGTCC AAGCCGGTGA TCGCCGCGGT CAGTGGCTAC GCGGTGGCCG GGGGACTGGA GCTGGCGTTG TGGTGCGATC TGCGGGTGGC CGAACAGGAC GCGGTATTCG GCGTGTTCTG TCGGCGGTGG GGCGTGCCGC TCATCGACGG CGGCACGGTG CGGCTGCCGC GGCTGATCGG CCACAGCCGG GCGATGGACA TGATCCTTAC CGGTCGTGCG GTGCAGGCCG ACGAGGCGCT GGCAATCGGG TTGGCGAATC GGGTGGTACC CAACGGACAA GCCCGGCAAG CAGCCGAGGA GTTGGCGGCC CAGCTGGCGG CGCTGCCACA ACAATGCCTG CGATCGGATC GCCTCTCAGC GCTTCAGCAA TGGGGCTTGC CCGAATCCGC GGCTCTCGAT CTGGAATTCG CCAGCATTTC CCGAGTGGCC GCCGAGGCGT TGGAGGGGGC CGGGCGCTTC GCCGCGGGTG CCGGTCGCCA TGGCGCCCAG GCGAGCCACA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A