SSGCID: Target Page

MyulA.00386.a

Enoyl-CoA hydratase, EchA5

CENTER ID:	MyulA.00386.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00386.a.A1.GE29687	Full length( MyulA.00386.a )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00386.a.A1.PS00764	Full length( MyulA.00386.a )		1	257

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133017
PATRIC ID:	fig\|362242.7.peg.955
RefSeq:	YP_904872.1
UniProt:	A0PM32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDLVQVERN GPVTTVIINR PQARNAVNGP TAAALYSAFA EFDRDESASV AVLCGNGGTF CAGADLKAFG TAEANAVHRT GPGPMGRSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA EQDSVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRAVQADEAL AIGLANRVVP NGQARQAAEE LAAQLAALPQ QCLRSDRLSA LQQWGLPESA ALDLEFASIS RVAAEALEGA GRFAAGAGRH GAQASHD

NT Sequence

GTGAGTGATC TAGTACAGGT CGAGCGCAAC GGTCCGGTAA CCACCGTGAT CATCAACCGG CCGCAGGCGC GCAACGCGGT CAACGGTCCG ACGGCTGCCG CGCTCTACTC GGCATTCGCC GAATTCGACC GGGATGAATC CGCTTCGGTG GCCGTCTTGT GCGGAAATGG CGGAACCTTC TGCGCGGGAG CGGATTTGAA GGCTTTCGGC ACCGCGGAGG CCAACGCCGT GCACCGGACG GGTCCGGGCC CGATGGGCCG GTCGCGAATG ATGCTGTCCA AGCCGGTGAT CGCCGCGGTC AGTGGCTACG CGGTGGCCGG GGGACTGGAG CTGGCGTTGT GGTGCGATCT GCGGGTGGCC GAACAGGACT CGGTATTCGG CGTGTTCTGT CGGCGGTGGG GCGTGCCGCT CATCGACGGC GGCACGGTGC GGCTGCCGCG GCTGATCGGC CACAGCCGGG CGATGGACAT GATCCTTACC GGTCGTGCGG TGCAGGCCGA CGAGGCGCTG GCAATCGGGT TGGCGAATCG GGTGGTACCC AACGGACAAG CCCGGCAAGC GGCCGAGGAG TTGGCGGCCC AGCTGGCGGC GCTGCCGCAA CAATGCCTGC GATCGGATCG CCTCTCAGCG CTTCAGCAAT GGGGCTTGCC CGAATCCGCG GCTCTCGATC TGGAATTCGC CAGCATTTCC CGAGTGGCCG CCGAGGCCTT GGAGGGGGCC GGGCGCTTCG CCGCGGGTGC CGGTCGCCAC GGCGCCCAGG CGAGCCACGA C

Details for MyulA.00386.a.A1.GE29687

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	85
PERCENT COVERAGE:	113

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGRS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDSVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAXXADE ALAIGLANRV VPNGQARQAA XELAAQLAAL PQXCLRSDRL SALXQWGLAR IRGSRSGIRQ HFPSGRRGLG XGRALRRXXR SPRRPXEPRL XXSTKXVLQP KLLXGSXXLT KPERXLXXXA XXRXXXX

Validated NT Sequence

ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagt gatctagtac aggtcgagcg caacggtccg gtaaccaccg tgatcatcaa ccggccgcag gcgcgcaacg cggtcaacgg tccgacggct gccgcgctct actcggcatt cgccgaattc gaccgggatg aatccgcttc ggtggccgtc ttgtgcggaa atggcggaac cttctgcgcg ggagcggatt tgaaggcttt cggcaccgcg gaggccaacg ccgtgcaccg gacgggtccg ggcccgatgg gccggtcgcg aatgatgctg tccaagccgg tgatcgccgc ggtcagtggc tacgcggtgg ccgggggact ggagctggcg ttgtggtgcg atctgcgggt ggccgaacag gactcggtat tcggcgtgtt ctgtcggcgg tggggcgtgc cgctcatcga cggcggcacg gtgcggctgc cgcggctgat cggccacagc cgggcgatgg acatgatcct taccggtcgt gcggngcang ccgacgaggc gctggcaatc gggttggcga atcgggtggt acccaacgga caagcccggc aagcggccga ngagttggcg gcccagctgg cggcgctgcc gcaacantgc ctgcgatcgg atcgcctctc agcgcttcan caatgggggc ttgcccgaat ccgcggctct cgatctggaa ttcgccagca tttcccgagt ggccgccgag gccttggnng gggccgggcg cttcgccgcn ggtgncggtc gccacggcgc ccnngcgagc cacgactaan aannagcacg aaacnagttc tgcagcccaa gcttctcgnn ggatccngnn tgctaacaaa gcccgaaagg nanctgantt nnnntgctgn cnnncgctna nnnannnncn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGRS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDSVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASHD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgat ctagtacagg tcgagcgcaa cggtccggta accaccgtga tcatcaaccg gccgcaggcg cgcaacgcgg tcaacggtcc gacggctgcc gcgctctact cggcattcgc cgaattcgac cgggatgaat ccgcttcggt ggccgtcttg tgcggaaatg gcggaacctt ctgcgcggga gcggatttga aggctttcgg caccgcggag gccaacgccg tgcaccggac gggtccgggc ccgatgggcc ggtcgcgaat gatgctgtcc aagccggtga tcgccgcggt cagtggctac gcggtggccg ggggactgga gctggcgttg tggtgcgatc tgcgggtggc cgaacaggac tcggtattcg gcgtgttctg tcggcggtgg ggcgtgccgc tcatcgacgg cggcacggtg cggctgccgc ggctgatcgg ccacagccgg gcgatggaca tgatccttac cggtcgtgcg gtgcaggccg acgaggcgct ggcaatcggg ttggcgaatc gggtggtacc caacggacaa gcccggcaag cggccgagga gttggcggcc cagctggcgg cgctgccgca acaatgcctg cgatcggatc gcctctcagc gcttcagcaa tggggcttgc ccgaatccgc ggctctcgat ctggaattcg ccagcatttc ccgagtggcc gccgaggcct tggagggggc cgggcgcttc gccgcgggtg ccggtcgcca cggcgcccag gcgagccacg acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MyulA.00386.a.A1.PS00764

PURIFICATION DATe:	9/8/2010
CONCENTRATION:	41.05mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	85
PERCENT COVERAGE:	113

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSDLVQVE RNGPVTTVII NRPQARNAVN GPTAAALYSA FAEFDRDESA SVAVLCGNGG TFCAGADLKA FGTAEANAVH RTGPGPMGRS RMMLSKPVIA AVSGYAVAGG LELALWCDLR VAEQDSVFGV FCRRWGVPLI DGGTVRLPRL IGHSRAMDMI LTGRAVQADE ALAIGLANRV VPNGQARQAA EELAAQLAAL PQQCLRSDRL SALQQWGLPE SAALDLEFAS ISRVAAEALE GAGRFAAGAG RHGAQASHD

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTGAT CTAGTACAGG TCGAGCGCAA CGGTCCGGTA ACCACCGTGA TCATCAACCG GCCGCAGGCG CGCAACGCGG TCAACGGTCC GACGGCTGCC GCGCTCTACT CGGCATTCGC CGAATTCGAC CGGGATGAAT CCGCTTCGGT GGCCGTCTTG TGCGGAAATG GCGGAACCTT CTGCGCGGGA GCGGATTTGA AGGCTTTCGG CACCGCGGAG GCCAACGCCG TGCACCGGAC GGGTCCGGGC CCGATGGGCC GGTCGCGAAT GATGCTGTCC AAGCCGGTGA TCGCCGCGGT CAGTGGCTAC GCGGTGGCCG GGGGACTGGA GCTGGCGTTG TGGTGCGATC TGCGGGTGGC CGAACAGGAC TCGGTATTCG GCGTGTTCTG TCGGCGGTGG GGCGTGCCGC TCATCGACGG CGGCACGGTG CGGCTGCCGC GGCTGATCGG CCACAGCCGG GCGATGGACA TGATCCTTAC CGGTCGTGCG GTGCAGGCCG ACGAGGCGCT GGCAATCGGG TTGGCGAATC GGGTGGTACC CAACGGACAA GCCCGGCAAG CGGCCGAGGA GTTGGCGGCC CAGCTGGCGG CGCTGCCGCA ACAATGCCTG CGATCGGATC GCCTCTCAGC GCTTCAGCAA TGGGGCTTGC CCGAATCCGC GGCTCTCGAT CTGGAATTCG CCAGCATTTC CCGAGTGGCC GCCGAGGCCT TGGAGGGGGC CGGGCGCTTC GCCGCGGGTG CCGGTCGCCA CGGCGCCCAG GCGAGCCACG ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA