MysmA.00781.b

Acetyl-CoA acetyltransferase

CENTER ID: MysmA.00781.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00781.b.A1.PS00613 Full length( MysmA.00781.b ) 1 403

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5573
RefSeq: YP_889954.1
UniProt: A0R466

Orthologues for Rv0859

Species % Identity % Coverage Clones Proteins Structures
leprae 95 100 0 0 0
ulcerans 93 100 1 0 0
avium 90 100 1 1 1
paratuberculosis 90 100 1 1 0
abscessus 87 99 1 0 0
smegmatis 86 100 0 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEAFIYEA IRTPRGKQRG GALNEIKPVN LVVGLIDELR VRFPNLDETL ISDLVLGVVS PVGDQGGDIA RTATLVANLP ETTGGVQLNR FCASGLEAVN IAAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWAGDPET NYRIGFVPQG IGADLIATIE GFSREDVDAY AARSQERAAA AWSGGYFSKS VVPVKDQNGL VVLDHDEHMR PGTTVESLGK LKPAFEGVGA MGGFDDVALQ KYHSIEKINH VHTGGNSSGI VDGAALVLIG SESAGKAAGL TPRARVVATA TSGADPVIML TGPTPATRKV LDRAGLTVDD IDLFELNEAF ASVVLKFQKD LGIPDEKLNV NGGAIAMGHP LGATGAMITG TMVDELERRG ARRALITLCV GGGMGVATII ERV
NT Sequence
ATGTCCGAAG AAGCCTTCAT CTACGAGGCT ATTCGTACGC CACGCGGCAA GCAGCGTGGC GGAGCCCTCA ACGAGATCAA GCCCGTCAAC CTGGTCGTCG GCCTGATCGA TGAACTGCGA GTCCGTTTCC CCAACCTGGA CGAGACCCTG ATCAGCGACC TCGTGCTCGG CGTGGTGTCG CCCGTGGGCG ACCAGGGCGG CGACATCGCC CGCACCGCGA CGCTGGTCGC GAACCTGCCG GAAACCACCG GTGGTGTGCA GCTCAACCGC TTCTGCGCGT CCGGCCTTGA GGCCGTGAAC ATCGCCGCGC AGAAGGTGCG CTCGGGCTGG GACGACCTGG TGCTCGCCGG CGGCGTGGAG TCCATGAGCC GCGTGCCGAT GGGTTCCGAC GGCGGCGCCT GGGCCGGTGA TCCCGAGACC AACTACCGCA TCGGTTTCGT GCCGCAGGGT ATCGGCGCCG ACCTGATCGC CACCATCGAG GGATTCTCCC GCGAGGACGT CGACGCCTAC GCCGCACGCT CGCAGGAGCG CGCCGCGGCG GCCTGGTCGG GCGGCTACTT CTCCAAGTCC GTGGTGCCCG TGAAGGACCA GAACGGCCTG GTCGTGCTGG ATCACGACGA GCACATGCGT CCCGGCACCA CCGTCGAGAG CCTGGGCAAG CTCAAGCCCG CATTCGAGGG TGTCGGCGCG ATGGGCGGCT TCGACGACGT GGCGCTGCAG AAGTACCACT CGATAGAGAA GATCAACCAC GTCCACACCG GCGGCAACAG CTCGGGCATC GTCGACGGCG CCGCGCTGGT GCTCATCGGC TCGGAAAGCG CGGGGAAGGC GGCCGGTTTG ACCCCGCGGG CGCGCGTCGT CGCCACCGCC ACCAGCGGTG CCGATCCGGT CATCATGCTC ACCGGCCCGA CGCCCGCTAC GCGCAAGGTG CTCGACCGGG CCGGTCTGAC GGTGGATGAC ATCGACCTGT TCGAGCTCAA TGAGGCGTTC GCCTCGGTGG TGCTGAAGTT CCAGAAGGAC CTTGGCATCC CCGACGAGAA GCTCAACGTC AACGGCGGCG CCATCGCGAT GGGCCACCCG CTGGGCGCCA CGGGCGCCAT GATCACCGGA ACCATGGTCG ACGAGCTCGA GCGTCGCGGC GCTCGGCGTG CGCTGATCAC GCTGTGCGTG GGCGGCGGCA TGGGCGTGGC CACCATCATC GAACGAGTC
Details for MysmA.00781.b.A1.PS00613
PURIFICATION DATe: 5/20/2010
CONCENTRATION: data unavailable
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 83
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQR GGALNEIKPV NLVVGLIDEL RVRFPNLDET LISDLVLGVV SPVGDQGGDI ARTATLVANL PETTGGVQLN RFCASGLEAV NIAAQKVRSG WDDLVLAGGV ESMSRVPMGS DGGAWAGDPE TNYRIGFVPQ GIGADLIATI EGFSREDVDA YAARSQERAA AAWSGGYFSK SVVPVKDQNG LVVLDHDEHM RPGTTVESLG KLKPAFEGVG AMGGFDDVAL XKYHSIEKIN HVHTGGNSSG IVDGAALVLI GSXSAGXXAG LXPRARVVAT ATSGADPVIM LTXPDXXTRX XRPGXLXVXD IDXXXSXXSX SXXXXXXXXX XXXXXXNGGX SXXXXXGXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgtttn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtccg aagaagcctt catctacgag gctattcgta cgccacgcgg caagcagcgt ggcggagccc tcaacgagat caagcccgtc aacctggtcg tcggcctgat cgatgaactg cgagtccgtt tccccaacct ggacgagacc ctgatcagcg acctcgtgct cggcgtggtg tcgcccgtgg gcgaccaggg cggcgacatc gcccgcaccg cgacgctggt cgcgaacctg ccggaaacca ccggtggtgt gcagctcaac cgcttctgcg cgtccggcct tgaggccgtg aacatcgccg cgcagaaggt gcgctcgggc tgggacgacc tggtgctcgc cggcggcgtg gagtccatga gccgcgtgcc gatgggttcc gacggcggcg cctgggccgg tgatcccgag accaactacc gcatcggttt cgtgccgcag ggtatcggcg ccgacctgat cgccaccatc gagggattct cccgcgagga cgtcgacgcc tacgccgcac gctcgcagga gcgcgccgcg gcggcctggt cgggcggcta cttctccaag tccgtggtgc ccgtgaagga ccagaacggc ctggtcgtgc tggatcacga cgagcacatg cgtcccggca ccaccgtcga gagcctgggc aagctcaagc ccgcattcga gggtgtcggc gcgatgggcg gcttcgacga cgtggcgctg canaagtacc actcgataga gaagatcaac cacgtccaca ccggcggcaa cagctcgggc atcgtcgacg gcgccgcgct ggtgctcatc ggctcnnaaa gcgcggggaa ngnggccggt ttgancccgc gggcgcgcgt cgtcgccacc gccaccagcg gtgccgatcc ngtcatcatg ctcaccnggc ccgacnnnnc tacncgcang gntcgaccgg gcngtctgan ggtggnngac atcgacnnnn cnanctcann nngttcnncn tcngngnnct naannnnnnn nnnttnnnnn tncncnnnnn cnnannncaa cggnggnncn tcnnnnnnnn nnncnnnngg nnnnnnnnna nnnnnnnnnn nnnnnnannn nnnnnnnnnn nnnnggntnn nnnnnnnnnn nc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQR GGALNEIKPV NLVVGLIDEL RVRFPNLDET LISDLVLGVV SPVGDQGGDI ARTATLVANL PETTGGVQLN RFCASGLEAV NIAAQKVRSG WDDLVLAGGV ESMSRVPMGS DGGAWAGDPE TNYRIGFVPQ GIGADLIATI EGFSREDVDA YAARSQERAA AAWSGGYFSK SVVPVKDQNG LVVLDHDEHM RPGTTVESLG KLKPAFEGVG AMGGFDDVAL QKYHSIEKIN HVHTGGNSSG IVDGAALVLI GSESAGKAAG LTPRARVVAT ATSGADPVIM LTGPTPATRK VLDRAGLTVD DIDLFELNEA FASVVLKFQK DLGIPDEKLN VNGGAIAMGH PLGATGAMIT GTMVDELERR GARRALITLC VGGGMGVATI IERV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGAAGAA GCCTTCATCT ACGAGGCTAT TCGTACGCCA CGCGGCAAGC AGCGTGGCGG AGCCCTCAAC GAGATCAAGC CCGTCAACCT GGTCGTCGGC CTGATCGATG AACTGCGAGT CCGTTTCCCC AACCTGGACG AGACCCTGAT CAGCGACCTC GTGCTCGGCG TGGTGTCGCC CGTGGGCGAC CAGGGCGGCG ACATCGCCCG CACCGCGACG CTGGTCGCGA ACCTGCCGGA AACCACCGGT GGTGTGCAGC TCAACCGCTT CTGCGCGTCC GGCCTTGAGG CCGTGAACAT CGCCGCGCAG AAGGTGCGCT CGGGCTGGGA CGACCTGGTG CTCGCCGGCG GCGTGGAGTC CATGAGCCGC GTGCCGATGG GTTCCGACGG CGGCGCCTGG GCCGGTGATC CCGAGACCAA CTACCGCATC GGTTTCGTGC CGCAGGGTAT CGGCGCCGAC CTGATCGCCA CCATCGAGGG ATTCTCCCGC GAGGACGTCG ACGCCTACGC CGCACGCTCG CAGGAGCGCG CCGCGGCGGC CTGGTCGGGC GGCTACTTCT CCAAGTCCGT GGTGCCCGTG AAGGACCAGA ACGGCCTGGT CGTGCTGGAT CACGACGAGC ACATGCGTCC CGGCACCACC GTCGAGAGCC TGGGCAAGCT CAAGCCCGCA TTCGAGGGTG TCGGCGCGAT GGGCGGCTTC GACGACGTGG CGCTGCAGAA GTACCACTCG ATAGAGAAGA TCAACCACGT CCACACCGGC GGCAACAGCT CGGGCATCGT CGACGGCGCC GCGCTGGTGC TCATCGGCTC GGAAAGCGCG GGGAAGGCGG CCGGTTTGAC CCCGCGGGCG CGCGTCGTCG CCACCGCCAC CAGCGGTGCC GATCCGGTCA TCATGCTCAC CGGCCCGACG CCCGCTACGC GCAAGGTGCT CGACCGGGCC GGTCTGACGG TGGATGACAT CGACCTGTTC GAGCTCAATG AGGCGTTCGC CTCGGTGGTG CTGAAGTTCC AGAAGGACCT TGGCATCCCC GACGAGAAGC TCAACGTCAA CGGCGGCGCC ATCGCGATGG GCCACCCGCT GGGCGCCACG GGCGCCATGA TCACCGGAAC CATGGTCGAC GAGCTCGAGC GTCGCGGCGC TCGGCGTGCG CTGATCACGC TGTGCGTGGG CGGCGGCATG GGCGTGGCCA CCATCATCGA ACGAGTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA