MyabA.00654.d

Probable acyl-coa thiolase FadA

CENTER ID: MyabA.00654.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00654.d.A1.GE30329 Full length( MyabA.00654.d ) 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.887
RefSeq: YP_001701598.1
UniProt: B1MIC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEAFIYEAV RTPRGKQRGG ALNEIKPLNL VVGLIEEIRH RHPDLDENLI SDVVLGVVSP VGDQGGDIAR TAVLAAGLPD TTGGVQLNRF CASGLEAINI GAQKVRSGWD DLVLAGGVES MSRVPMGSDG GAWASDPATN YDVSFVPQGI GADLIATIEG FTRDDVDAYA ARSQERAAAA WSGGYFAKSV VPVKDQNGLL VLDHDEHMRP GTTAADLGKL KPAFEGLAAL GGFDDVARIK YHYVEKINHV HTGGNSSGIV DGAALVLIGS EEAGKSQGLT PRARIVATAT SGSEPLIMLT GPTPATKKVL DRAGLTVDDI DLFELNEAFA SVVLKFQKDL NIPDEKLNVN GGAIAMGHPL GATGAMITGT MVDELERRGL KRALITLCVG GGMGVATIIE RV
NT Sequence
ATGACTGAAG CATTCATCTA CGAGGCCGTC CGTACGCCTC GCGGCAAGCA GCGTGGCGGC GCTCTCAACG AGATCAAGCC GCTGAACCTG GTGGTCGGCC TCATCGAGGA GATCCGGCAC CGTCACCCCG ACCTGGACGA GAACCTGATC AGCGATGTCG TGCTGGGTGT CGTGTCCCCG GTCGGCGACC AGGGTGGCGA TATCGCCCGC ACCGCGGTGC TGGCGGCCGG CCTGCCCGAC ACCACCGGTG GTGTCCAGCT CAACCGTTTC TGTGCCTCGG GCCTGGAGGC CATCAACATC GGCGCACAGA AGGTGCGTTC CGGCTGGGAC GACCTGGTGC TCGCCGGTGG TGTGGAGTCG ATGTCGCGCG TGCCGATGGG CAGCGACGGC GGCGCGTGGG CGTCGGACCC GGCCACCAAC TACGACGTGT CCTTCGTTCC GCAGGGCATC GGTGCCGACT TGATCGCCAC TATCGAGGGC TTCACCCGTG ACGATGTGGA CGCCTACGCC GCCCGCTCGC AGGAGCGTGC CGCGGCCGCG TGGTCCGGTG GCTACTTCGC CAAGTCCGTT GTGCCGGTCA AGGATCAGAA CGGGCTGCTC GTTCTCGATC ACGACGAGCA CATGCGCCCG GGCACCACCG CGGCAGACCT GGGCAAGCTC AAGCCCGCGT TCGAGGGCCT GGCCGCGCTC GGCGGCTTCG ACGACGTGGC GCGCATCAAG TACCACTACG TCGAGAAGAT CAACCACGTC CACACCGGTG GCAACAGCTC CGGCATCGTC GACGGCGCCG CCCTGGTGCT GATCGGTTCC GAGGAGGCCG GCAAGTCGCA GGGCCTGACC CCGCGCGCTC GTATCGTGGC CACCGCCACC TCCGGCAGCG AGCCGCTCAT CATGCTGACC GGTCCCACCC CGGCGACCAA GAAGGTGCTC GACCGTGCCG GGCTGACCGT GGACGACATC GACCTGTTCG AGCTCAACGA GGCCTTCGCC TCGGTGGTCC TGAAGTTCCA GAAGGACCTG AACATCCCGG ACGAGAAGCT CAACGTCAAC GGCGGCGCCA TCGCGATGGG CCACCCGCTG GGCGCCACCG GCGCCATGAT CACCGGCACC ATGGTCGACG AGCTGGAGCG TCGCGGCCTC AAGCGTGCCC TCATCACCCT GTGCGTCGGC GGCGGCATGG GTGTAGCCAC CATCATCGAG CGAGTC
Details for MyabA.00654.d.A1.GE30329
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTEAFIYEA VRTPRGKQRG GALNEIKPLN LVVGLIEEIR HRHPDLDENL ISDVVLGVVS PVGDQGGDIA RTAVLAAGLP DTTGGVQLNR FCASGLEAIN IGAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWASDPAT NYDVSFVPQG IGADLIATIE GFTRDDVDAY AARSQERAAA AWSGGYFAKS VVPVKDQNGL LVLDHDEHMR PGTTAADLGK LKPAFEGLAA LGGFXDVARI KYHYVEKINH VHTGGNSSXI VDGAXLVLIG SXXAGXSXGL TPRARIVXXR XLRXXAAHHA XXVPXPAXXX XSTGXAXXGR XXXXXXXXXX XXXXXXXXXX XXXXXXXXVX XXXXXXXXXG XXXXXXXXX
Validated NT Sequence
ttntttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgactgaag cattcatcta cgaggccgtc cgtacgcctc gcggcaagca gcgtggcggc gctctcaacg agatcaagcc gctgaacctg gtggtcggcc tcatcgagga gatccggcac cgtcaccccg acctggacga gaacctgatc agcgatgtcg tgctgggtgt cgtgtccccg gtcggcgacc agggtggcga tatcgcccgc accgcggtgc tggcggccgg cctgcccgac accaccggtg gtgtccagct caaccgtttc tgtgcctcgg gcctggaggc catcaacatc ggcgcacaga aggtgcgttc cggctgggac gacctggtgc tcgccggtgg tgtggagtcg atgtcgcgcg tgccgatggg cagcgacggc ggcgcgtggg cgtcggaccc ggccaccaac tacgacgtgt ccttcgttcc gcagggcatc ggtgccgact tgatcgccac tatcgagggc ttcacccgtg acgatgtgga cgcctacgcc gcccgctcgc aggagcgtgc cgcggccgcg tggtccggtg gctacttcgc caagtccgtt gtgccggtca aggatcagaa cgggctgctc gttctcgatc acgacgagca catgcgcccg ggcaccaccg cggcagacct gggcaagctc aagcccgcgt tcgagggcct ggccgcgctc ggcggcttcn acgacgtggc gcgcatcaag taccactacg tcgagaagat caaccacgtc cacaccggtg gnaacagctc cngnatcgtc gacggcgccn ccctggtgct gatcggntcc gangangccg gnnagtcgcn nggcctgacc ccgcgcgctc gtatcgtggn cnnccgccnc ctccggcanc nagccgctca tcatgctgnn cnggtcccnn ccccggcgan caanangngc tcgacnggnn gggcngancn tggncganat cnanctnnnn nnncnnaann nnncnntnnn cntnnnnnnn nnantncnnn nnnnntgann ntccnnnnnn nnnnnntnan ngtcnangng gnnnnntnnn nnnnnnnnnn nnngggnnnc nnnnnnnnnn ncnnnnnnnn nannnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEAFIYEA VRTPRGKQRG GALNEIKPLN LVVGLIEEIR HRHPDLDENL ISDVVLGVVS PVGDQGGDIA RTAVLAAGLP DTTGGVQLNR FCASGLEAIN IGAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWASDPAT NYDVSFVPQG IGADLIATIE GFTRDDVDAY AARSQERAAA AWSGGYFAKS VVPVKDQNGL LVLDHDEHMR PGTTAADLGK LKPAFEGLAA LGGFDDVARI KYHYVEKINH VHTGGNSSGI VDGAALVLIG SEEAGKSQGL TPRARIVATA TSGSEPLIML TGPTPATKKV LDRAGLTVDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP LGATGAMITG TMVDELERRG LKRALITLCV GGGMGVATII ERV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgaagca ttcatctacg aggccgtccg tacgcctcgc ggcaagcagc gtggcggcgc tctcaacgag atcaagccgc tgaacctggt ggtcggcctc atcgaggaga tccggcaccg tcaccccgac ctggacgaga acctgatcag cgatgtcgtg ctgggtgtcg tgtccccggt cggcgaccag ggtggcgata tcgcccgcac cgcggtgctg gcggccggcc tgcccgacac caccggtggt gtccagctca accgtttctg tgcctcgggc ctggaggcca tcaacatcgg cgcacagaag gtgcgttccg gctgggacga cctggtgctc gccggtggtg tggagtcgat gtcgcgcgtg ccgatgggca gcgacggcgg cgcgtgggcg tcggacccgg ccaccaacta cgacgtgtcc ttcgttccgc agggcatcgg tgccgacttg atcgccacta tcgagggctt cacccgtgac gatgtggacg cctacgccgc ccgctcgcag gagcgtgccg cggccgcgtg gtccggtggc tacttcgcca agtccgttgt gccggtcaag gatcagaacg ggctgctcgt tctcgatcac gacgagcaca tgcgcccggg caccaccgcg gcagacctgg gcaagctcaa gcccgcgttc gagggcctgg ccgcgctcgg cggcttcgac gacgtggcgc gcatcaagta ccactacgtc gagaagatca accacgtcca caccggtggc aacagctccg gcatcgtcga cggcgccgcc ctggtgctga tcggttccga ggaggccggc aagtcgcagg gcctgacccc gcgcgctcgt atcgtggcca ccgccacctc cggcagcgag ccgctcatca tgctgaccgg tcccaccccg gcgaccaaga aggtgctcga ccgtgccggg ctgaccgtgg acgacatcga cctgttcgag ctcaacgagg ccttcgcctc ggtggtcctg aagttccaga aggacctgaa catcccggac gagaagctca acgtcaacgg cggcgccatc gcgatgggcc acccgctggg cgccaccggc gccatgatca ccggcaccat ggtcgacgag ctggagcgtc gcggcctcaa gcgtgccctc atcaccctgt gcgtcggcgg cggcatgggt gtagccacca tcatcgagcg agtctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa