MypaA.00781.c

FadA_1

CENTER ID: MypaA.00781.c
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00781.c.A1.GE29508 Full length( MypaA.00781.c ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00781.c.A1.PW29406 Full length( MypaA.00781.c ) 1 403

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.831
RefSeq: NP_959723.1
UniProt: Q742P6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEAFIYEA IRTPRGKQKN GSLTEVKPLN LVVGLVDELR RRYPDLDESL ISDMILGVVS PVGDQGGDIA RTAVLAAGLP ETTGGVQLNR FCASGLEAVN TAAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWATDPET NYRIGFVPQG IGADLIATLE GFSREDVDAY ALRSQQKAAA AWSGGYFAKS VVPVRDQNGL VILDHDEHMR PDTTMEGLAK LKTAFDGVGE MGGFDDVALQ KYHWVEKINH VHTGGNSSGI VDGAALVLVG SEKAGKSQGL TPRARIVATA TSGSDPVIML TGPTPATRKV LDRAGLTIDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP LGATGAMITG TMVDELERRN ARRALITLCI GGGMGVATII ERV
NT Sequence
ATGTCCGAAG AAGCTTTTAT CTACGAAGCC ATCCGCACAC CGCGGGGCAA GCAGAAGAAC GGCTCGCTGA CCGAGGTGAA GCCGCTCAAC CTGGTGGTAG GCCTGGTCGA CGAGCTGCGC CGGCGCTACC CCGACCTGGA CGAGAGCCTG ATCAGCGACA TGATCCTGGG TGTCGTGTCC CCGGTCGGCG ACCAGGGTGG CGACATCGCC CGCACCGCGG TGCTGGCCGC CGGCCTGCCC GAGACCACCG GCGGCGTGCA GCTCAACCGG TTCTGCGCCT CGGGCCTCGA GGCCGTCAAC ACCGCCGCCC AGAAGGTGCG CTCCGGCTGG GACGACCTGG TGCTGGCCGG CGGTGTGGAG TCGATGAGCC GGGTCCCGAT GGGCTCCGAC GGCGGCGCCT GGGCCACCGA CCCGGAGACC AACTACCGGA TCGGCTTCGT GCCGCAGGGC ATCGGCGCCG ATTTGATCGC CACCCTCGAG GGCTTCTCCC GCGAGGACGT CGACGCCTAC GCGCTGCGCA GCCAGCAGAA GGCCGCCGCG GCCTGGTCGG GCGGCTACTT CGCCAAGTCG GTGGTGCCGG TGCGTGACCA GAACGGTCTG GTCATCCTCG ACCACGACGA GCACATGCGG CCCGACACCA CGATGGAGGG CCTGGCCAAG CTGAAGACCG CGTTCGACGG TGTCGGCGAG ATGGGCGGCT TCGACGACGT GGCGCTGCAG AAGTACCACT GGGTCGAGAA GATCAACCAC GTCCACACCG GCGGCAACAG CTCGGGCATC GTCGACGGCG CCGCGCTGGT GCTGGTCGGC AGCGAAAAGG CCGGCAAGTC GCAGGGGTTG ACCCCGCGGG CCCGCATCGT GGCCACCGCG ACCAGCGGCT CGGACCCGGT CATCATGCTC ACCGGCCCCA CCCCGGCCAC CCGCAAGGTG CTCGACCGGG CCGGCCTGAC GATCGACGAC ATCGATCTGT TCGAACTGAA CGAGGCGTTC GCGTCGGTGG TGCTGAAGTT CCAGAAAGAC CTCAACATTC CCGACGAGAA GCTCAACGTC AACGGCGGCG CCATCGCGAT GGGCCACCCG CTGGGCGCCA CCGGCGCCAT GATCACCGGC ACCATGGTCG ACGAGCTCGA GCGCCGCAAC GCGCGTCGCG CCCTGATCAC GCTGTGCATC GGCGGCGGCA TGGGTGTCGC CACCATCATC GAGAGGGTT
Details for MypaA.00781.c.A1.GE29508
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSEEAFIYEA IRTPRGKQKN GSLTEVKPLN LVVGLVDELR RRYPDLDESL ISDMILGVVS PVGDQGGDIA RTAVLAAGLP ETTGGVQLNR FCASGLEAVN TAAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWATDPET NYRIGFVPQG IGADLIATLE GFSREDVDAY ALRSQQKAAA AWSGGYFAKS VVPVRDQNGL VILDHDEHMR PDTTMEGLAK LKTAFDGVGE MGGFDDVALQ KYHWVEKINH VHTGGNSSGI VDGAALVLVG SEKAGKSQGL TPRARIVATA TSGSDPVIML TGPTPATRKV LDRAGLTIDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP LGATGAMITG TMVDELERRN ARRALITLCI GGGMGVATII ER
Validated NT Sequence
accctctcga tgatggtggc gacacccatg ccgccgccga tgcacagcgt gatcagggcg cgacgcgcgt tgcggcgctc gagctcgtcg accatggtgc cggtgatcat ggcgccggtg gcgcccagcg ggtggcccat cgcgatggcg ccgccgttga cgttgagctt ctcgtcggga atgttgaggt ctttctggaa cttcagcacc accgacgcga acgcctcgtt cagttcgaac agatcgatgt cgtcgatcgt caggccggcc cggtcgagca ccttgcgggt ggccggggtg gggccggtga gcatgatgac cgggtccgag ccgctggtcg cggtggccac gatgcgggcc cgcggggtca acccctgcga cttgccggcc ttttcgctgc cgaccagcac cagcgcggcg ccgtcgacga tgcccgagct gttgccgccg gtgtggacgt ggttgatctt ctcgacccag tggtacttct gcagcgccac gtcgtcgaag ccgcccatct cgccgacacc gtcgaacgcg gtcttcagct tggccaggcc ctccatcgtg gtgtcgggcc gcatgtgctc gtcgtggtcg aggatgacca gaccgttctg gtcacgcacc ggcaccaccg acttggcgaa gtagccgccc gaccaggccg cggcggcctt ctgctggctg cgcagcgcgt aggcgtcgac gtcctcgcgg gagaagccct cgagggtggc gatcaaatcg gcgccgatgc cctgcggcac gaagccgatc cggtagttgg tctccgggtc ggtggcccag gcgccgccgt cggagcccat cgggacccgg ctcatcgact ccacaccgcc ggccagcacc aggtcgtccc agccggagcg caccttctgg gcggcggtgt tgacggcctc gaggcccgag gcgcagaacc ggttgagctg cacgccgccg gtggtctcgg gcaggccggc ggccagcacc gcggtgcggg cgatgtcgcc accctggtcg ccgaccgggg acacgacacc caggatcatg tcgctgatca ggctctcgtc caggtcgggg tagcgccggc gcagctcgtc gaccaggcct accaccaggt tgagcggctt cacctcggtc agcgagccgt tcttctgctt gccccgcggt gtgcggatgg cttcgtagat aaaagcttct tcggacatcg aaccaggacc ctgggtctga gcttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQK NGSLTEVKPL NLVVGLVDEL RRRYPDLDES LISDMILGVV SPVGDQGGDI ARTAVLAAGL PETTGGVQLN RFCASGLEAV NTAAQKVRSG WDDLVLAGGV ESMSRVPMGS DGGAWATDPE TNYRIGFVPQ GIGADLIATL EGFSREDVDA YALRSQQKAA AAWSGGYFAK SVVPVRDQNG LVILDHDEHM RPDTTMEGLA KLKTAFDGVG EMGGFDDVAL QKYHWVEKIN HVHTGGNSSG IVDGAALVLV GSEKAGKSQG LTPRARIVAT ATSGSDPVIM LTGPTPATRK VLDRAGLTID DIDLFELNEA FASVVLKFQK DLNIPDEKLN VNGGAIAMGH PLGATGAMIT GTMVDELERR NARRALITLC IGGGMGVATI IERV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaagaa gcttttatct acgaagccat ccgcacaccg cggggcaagc agaagaacgg ctcgctgacc gaggtgaagc cgctcaacct ggtggtaggc ctggtcgacg agctgcgccg gcgctacccc gacctggacg agagcctgat cagcgacatg atcctgggtg tcgtgtcccc ggtcggcgac cagggtggcg acatcgcccg caccgcggtg ctggccgccg gcctgcccga gaccaccggc ggcgtgcagc tcaaccggtt ctgcgcctcg ggcctcgagg ccgtcaacac cgccgcccag aaggtgcgct ccggctggga cgacctggtg ctggccggcg gtgtggagtc gatgagccgg gtcccgatgg gctccgacgg cggcgcctgg gccaccgacc cggagaccaa ctaccggatc ggcttcgtgc cgcagggcat cggcgccgat ttgatcgcca ccctcgaggg cttctcccgc gaggacgtcg acgcctacgc gctgcgcagc cagcagaagg ccgccgcggc ctggtcgggc ggctacttcg ccaagtcggt ggtgccggtg cgtgaccaga acggtctggt catcctcgac cacgacgagc acatgcggcc cgacaccacg atggagggcc tggccaagct gaagaccgcg ttcgacggtg tcggcgagat gggcggcttc gacgacgtgg cgctgcagaa gtaccactgg gtcgagaaga tcaaccacgt ccacaccggc ggcaacagct cgggcatcgt cgacggcgcc gcgctggtgc tggtcggcag cgaaaaggcc ggcaagtcgc aggggttgac cccgcgggcc cgcatcgtgg ccaccgcgac cagcggctcg gacccggtca tcatgctcac cggccccacc ccggccaccc gcaaggtgct cgaccgggcc ggcctgacga tcgacgacat cgatctgttc gaactgaacg aggcgttcgc gtcggtggtg ctgaagttcc agaaagacct caacattccc gacgagaagc tcaacgtcaa cggcggcgcc atcgcgatgg gccacccgct gggcgccacc ggcgccatga tcaccggcac catggtcgac gagctcgagc gccgcaacgc gcgtcgcgcc ctgatcacgc tgtgcatcgg cggcggcatg ggtgtcgcca ccatcatcga gagggttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MypaA.00781.c.A1.PW29406
PURIFICATION DATe: 1/18/2011
CONCENTRATION: 29.82mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSEEAFIYEA IRTPRGKQKN GSLTEVKPLN LVVGLVDELR RRYPDLDESL ISDMILGVVS PVGDQGGDIA RTAVLAAGLP ETTGGVQLNR FCASGLEAVN TAAQKVRSGW DDLVLAGGVE SMSRVPMGSD GGAWATDPET NYRIGFVPQG IGADLIATLE GFSREDVDAY ALRSQQKAAA AWSGGYFAKS VVPVRDQNGL VILDHDEHMR PDTTMEGLAK LKTAFDGVGE MGGFDDVALQ KYHWVEKINH VHTGGNSSGI VDGAALVLVG SEKAGKSQGL TPRARIVATA TSGSDPVIML TGPTPATRKV LDRAGLTIDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP LGATGAMITG TMVDELERRN ARRALITLCI GGGMGVATII ER
Validated NT Sequence
accctctcga tgatggtggc gacacccatg ccgccgccga tgcacagcgt gatcagggcg cgacgcgcgt tgcggcgctc gagctcgtcg accatggtgc cggtgatcat ggcgccggtg gcgcccagcg ggtggcccat cgcgatggcg ccgccgttga cgttgagctt ctcgtcggga atgttgaggt ctttctggaa cttcagcacc accgacgcga acgcctcgtt cagttcgaac agatcgatgt cgtcgatcgt caggccggcc cggtcgagca ccttgcgggt ggccggggtg gggccggtga gcatgatgac cgggtccgag ccgctggtcg cggtggccac gatgcgggcc cgcggggtca acccctgcga cttgccggcc ttttcgctgc cgaccagcac cagcgcggcg ccgtcgacga tgcccgagct gttgccgccg gtgtggacgt ggttgatctt ctcgacccag tggtacttct gcagcgccac gtcgtcgaag ccgcccatct cgccgacacc gtcgaacgcg gtcttcagct tggccaggcc ctccatcgtg gtgtcgggcc gcatgtgctc gtcgtggtcg aggatgacca gaccgttctg gtcacgcacc ggcaccaccg acttggcgaa gtagccgccc gaccaggccg cggcggcctt ctgctggctg cgcagcgcgt aggcgtcgac gtcctcgcgg gagaagccct cgagggtggc gatcaaatcg gcgccgatgc cctgcggcac gaagccgatc cggtagttgg tctccgggtc ggtggcccag gcgccgccgt cggagcccat cgggacccgg ctcatcgact ccacaccgcc ggccagcacc aggtcgtccc agccggagcg caccttctgg gcggcggtgt tgacggcctc gaggcccgag gcgcagaacc ggttgagctg cacgccgccg gtggtctcgg gcaggccggc ggccagcacc gcggtgcggg cgatgtcgcc accctggtcg ccgaccgggg acacgacacc caggatcatg tcgctgatca ggctctcgtc caggtcgggg tagcgccggc gcagctcgtc gaccaggcct accaccaggt tgagcggctt cacctcggtc agcgagccgt tcttctgctt gccccgcggt gtgcggatgg cttcgtagat aaaagcttct tcggacatcg aaccaggacc ctgggtctga gcttccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQK NGSLTEVKPL NLVVGLVDEL RRRYPDLDES LISDMILGVV SPVGDQGGDI ARTAVLAAGL PETTGGVQLN RFCASGLEAV NTAAQKVRSG WDDLVLAGGV ESMSRVPMGS DGGAWATDPE TNYRIGFVPQ GIGADLIATL EGFSREDVDA YALRSQQKAA AAWSGGYFAK SVVPVRDQNG LVILDHDEHM RPDTTMEGLA KLKTAFDGVG EMGGFDDVAL QKYHWVEKIN HVHTGGNSSG IVDGAALVLV GSEKAGKSQG LTPRARIVAT ATSGSDPVIM LTGPTPATRK VLDRAGLTID DIDLFELNEA FASVVLKFQK DLNIPDEKLN VNGGAIAMGH PLGATGAMIT GTMVDELERR NARRALITLC IGGGMGVATI IERV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGAAGAA GCTTTTATCT ACGAAGCCAT CCGCACACCG CGGGGCAAGC AGAAGAACGG CTCGCTGACC GAGGTGAAGC CGCTCAACCT GGTGGTAGGC CTGGTCGACG AGCTGCGCCG GCGCTACCCC GACCTGGACG AGAGCCTGAT CAGCGACATG ATCCTGGGTG TCGTGTCCCC GGTCGGCGAC CAGGGTGGCG ACATCGCCCG CACCGCGGTG CTGGCCGCCG GCCTGCCCGA GACCACCGGC GGCGTGCAGC TCAACCGGTT CTGCGCCTCG GGCCTCGAGG CCGTCAACAC CGCCGCCCAG AAGGTGCGCT CCGGCTGGGA CGACCTGGTG CTGGCCGGCG GTGTGGAGTC GATGAGCCGG GTCCCGATGG GCTCCGACGG CGGCGCCTGG GCCACCGACC CGGAGACCAA CTACCGGATC GGCTTCGTGC CGCAGGGCAT CGGCGCCGAT TTGATCGCCA CCCTCGAGGG CTTCTCCCGC GAGGACGTCG ACGCCTACGC GCTGCGCAGC CAGCAGAAGG CCGCCGCGGC CTGGTCGGGC GGCTACTTCG CCAAGTCGGT GGTGCCGGTG CGTGACCAGA ACGGTCTGGT CATCCTCGAC CACGACGAGC ACATGCGGCC CGACACCACG ATGGAGGGCC TGGCCAAGCT GAAGACCGCG TTCGACGGTG TCGGCGAGAT GGGCGGCTTC GACGACGTGG CGCTGCAGAA GTACCACTGG GTCGAGAAGA TCAACCACGT CCACACCGGC GGCAACAGCT CGGGCATCGT CGACGGCGCC GCGCTGGTGC TGGTCGGCAG CGAAAAGGCC GGCAAGTCGC AGGGGTTGAC CCCGCGGGCC CGCATCGTGG CCACCGCGAC CAGCGGCTCG GACCCGGTCA TCATGCTCAC CGGCCCCACC CCGGCCACCC GCAAGGTGCT CGACCGGGCC GGCCTGACGA TCGACGACAT CGATCTGTTC GAACTGAACG AGGCGTTCGC GTCGGTGGTG CTGAAGTTCC AGAAAGACCT CAACATTCCC GACGAGAAGC TCAACGTCAA CGGCGGCGCC ATCGCGATGG GCCACCCGCT GGGCGCCACC GGCGCCATGA TCACCGGCAC CATGGTCGAC GAGCTCGAGC GCCGCAACGC GCGTCGCGCC CTGATCACGC TGTGCATCGG CGGCGGCATG GGTGTCGCCA CCATCATCGA GAGGGTTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA