MyulA.00654.c

Acyl-CoA thiolase FadA

CENTER ID: MyulA.00654.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00654.c.A1.GE29721 Full length( MyulA.00654.c ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.417
RefSeq: YP_904502.1
UniProt: A0PL12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEEAFIYEA IRTPRGKQKY GSLNEVKPIS LVVGLIEELR KRNPDLDENL ISDVILGCVS PVGDQGGDIA RAAVLASGMP VTSGGVQLNR FCASGLEAVN MAAQKVRSGW DDLVLAGGVE SMSRVPMGAD GGAMGLDPVT NYDVMFVPQG IGADLIATIE GFSRDDVDAY ALRSQEKAAE AWSGGYFAKS VVPVRDQNGL LILDHDEHMR PETTKEGLGK LKSAFEGLAA MGGFDDVALQ KYHWVEKINH VHTGGNSSGI VDGAAIVMVG SEKAGAAINA TPRARIVATA TSGADPVIML TGPTPAARKV LDRAGLTVDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP LGATGAMILG TMVDELERRN ARRALVTLCI GGGMGAATII ERV
NT Sequence
ATGTCCGAAG AAGCCTTCAT TTATGAGGCC ATCCGCACGC CGCGTGGCAA GCAAAAGTAC GGATCGCTAA ACGAGGTCAA GCCGATAAGC CTGGTCGTTG GCCTGATCGA GGAGCTGCGC AAGCGCAACC CTGACCTCGA CGAGAACCTG ATCAGCGACG TCATCCTGGG CTGCGTGTCG CCCGTCGGTG ACCAGGGCGG CGACATCGCC CGCGCCGCGG TGCTGGCCTC CGGGATGCCG GTCACCTCCG GTGGCGTGCA GCTCAACCGG TTCTGCGCAT CGGGTCTGGA AGCGGTGAAC ATGGCCGCCC AGAAGGTGCG TTCCGGCTGG GATGACCTGG TGCTGGCCGG TGGTGTGGAG TCGATGAGCC GGGTGCCGAT GGGTGCCGAC GGCGGCGCCA TGGGGCTTGA CCCGGTCACC AACTACGACG TCATGTTTGT TCCGCAGGGC ATCGGCGCCG ACCTGATTGC CACCATCGAG GGCTTCTCCC GCGACGACGT CGACGCCTAT GCGCTGCGTA GCCAGGAGAA GGCCGCCGAG GCGTGGTCGG GCGGCTACTT CGCCAAGTCC GTGGTGCCGG TCCGCGACCA AAACGGTCTG CTGATCCTCG ACCACGATGA GCACATGCGG CCCGAGACCA CCAAGGAAGG CCTGGGCAAG CTCAAGTCGG CCTTCGAGGG CCTGGCGGCG ATGGGCGGTT TCGACGACGT CGCGCTGCAG AAGTACCACT GGGTGGAAAA GATCAACCAC GTGCACACCG GCGGCAACAG CTCCGGCATC GTCGATGGCG CCGCGATCGT GATGGTCGGC AGCGAAAAGG CGGGGGCAGC GATCAACGCG ACCCCGCGCG CCCGCATCGT CGCCACCGCG ACCAGTGGCG CCGACCCGGT CATCATGCTG ACCGGCCCGA CCCCTGCCGC CCGCAAGGTG CTCGACCGCG CCGGCCTGAC GGTTGATGAC ATCGATCTGT TCGAGCTGAA CGAGGCGTTC GCGTCGGTGG TGCTGAAGTT CCAGAAGGAC CTCAACATCC CCGACGAGAA GCTCAACGTC AACGGTGGGG CCATCGCGAT GGGTCACCCG CTGGGGGCGA CCGGCGCGAT GATCCTGGGC ACCATGGTCG ACGAGCTGGA GCGCCGCAAC GCCCGGCGCG CATTGGTCAC GCTTTGTATT GGTGGCGGCA TGGGTGCCGC GACGATCATC GAGAGGGTT
Details for MyulA.00654.c.A1.GE29721
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 83
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQK YGSLNEVKPI SLVVGLIEEL RKRNPDLDEN LISDVILGCV SPVGDQGGDI ARAAVLASGM PVTSGGVQLN RFCASGLEAV NMAAQKVRSG WDDLVLAGGV ESMSRVPMGA DGGAMGLDPV TNYDVMFVPQ GIGADLIATI EGFSRDDVDA YALRSQEKAA EAWSGGYFAK SVVPVRDQNG LLILDHDEHM RPETTKEGLG KLKSAFEGLA AMGGFDDVAL QKYHWVEKIN HVHTGGXSSG IVDGAAIVMV GSEXXGAAIN ATPRARIVXT XTXXXXPVIM LXXXXPXXXX XXRXXXXXXL MXSIXXXXXX XXXXX
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtccg aagaagcctt catttatgag gccatccgca cgccgcgtgg caagcaaaag tacggatcgc taaacgaggt caagccgata agcctggtcg ttggcctgat cgaggagctg cgcaagcgca accctgacct cgacgagaac ctgatcagcg acgtcatcct gggctgcgtg tcgcccgtcg gtgaccaggg cggcgacatc gcccgcgccg cggtgctggc ctccgggatg ccggtcacct ccggtggcgt gcagctcaac cggttctgcg catcgggtct ggaagcggtg aacatggccg cccagaaggt gcgttccggc tgggatgacc tggtgctggc cggtggtgtg gagtcgatga gccgggtgcc gatgggtgcc gacggcggcg ccatggggct tgacccggtc accaactacg acgtcatgtt tgttccgcag ggcatcggcg ccgacctgat tgccaccatc gagggcttct cccgcgacga cgtcgacgcc tatgcgctgc gtagccagga gaaggccgcc gaggcgtggt cgggcggcta cttcgccaag tccgtggtgc cggtccgcga ccaaaacggt ctgctgatcc tcgaccacga tgagcacatg cggcccgaga ccaccaagga aggcctgggc aagctcaagt cggccttcga gggcctggcg gcgatgggcg gtttcgacga cgtcgcgctg cagaagtacc actgggtgga aaagatcaac cacgtgcaca ccggcggcna cagctccggc atcgtcgatg gngccgcgat cgtgatggtc ggnagcgaaa angnnggggc agcgatcaac gcgaccccgc gcgcccgcat cgtcnccacc ncgaccagng nngnnnaccc ngtcatcatg ctgancnngn ccnacccnnn nnnnncnnnn nnnnntcgan nnnnncngnn ntnnnnnttg atgnnntcga tctgntnnnn cngangnnnt ncnnnncnnn ggnnntgaan ntncnnnnnn annnnnnnnt cccnannnnn nnnnnnncnn nnnanngnng gggnnnnnnn nnnnnnnnnn ncnnnnnngn nnnnnnnnnn nnnnnnnnnn nnnnnncnnn gnnnncnnnn nnnnnnnnnn nnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEEAFIYE AIRTPRGKQK YGSLNEVKPI SLVVGLIEEL RKRNPDLDEN LISDVILGCV SPVGDQGGDI ARAAVLASGM PVTSGGVQLN RFCASGLEAV NMAAQKVRSG WDDLVLAGGV ESMSRVPMGA DGGAMGLDPV TNYDVMFVPQ GIGADLIATI EGFSRDDVDA YALRSQEKAA EAWSGGYFAK SVVPVRDQNG LLILDHDEHM RPETTKEGLG KLKSAFEGLA AMGGFDDVAL QKYHWVEKIN HVHTGGNSSG IVDGAAIVMV GSEKAGAAIN ATPRARIVAT ATSGADPVIM LTGPTPAARK VLDRAGLTVD DIDLFELNEA FASVVLKFQK DLNIPDEKLN VNGGAIAMGH PLGATGAMIL GTMVDELERR NARRALVTLC IGGGMGAATI IERV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaagaa gccttcattt atgaggccat ccgcacgccg cgtggcaagc aaaagtacgg atcgctaaac gaggtcaagc cgataagcct ggtcgttggc ctgatcgagg agctgcgcaa gcgcaaccct gacctcgacg agaacctgat cagcgacgtc atcctgggct gcgtgtcgcc cgtcggtgac cagggcggcg acatcgcccg cgccgcggtg ctggcctccg ggatgccggt cacctccggt ggcgtgcagc tcaaccggtt ctgcgcatcg ggtctggaag cggtgaacat ggccgcccag aaggtgcgtt ccggctggga tgacctggtg ctggccggtg gtgtggagtc gatgagccgg gtgccgatgg gtgccgacgg cggcgccatg gggcttgacc cggtcaccaa ctacgacgtc atgtttgttc cgcagggcat cggcgccgac ctgattgcca ccatcgaggg cttctcccgc gacgacgtcg acgcctatgc gctgcgtagc caggagaagg ccgccgaggc gtggtcgggc ggctacttcg ccaagtccgt ggtgccggtc cgcgaccaaa acggtctgct gatcctcgac cacgatgagc acatgcggcc cgagaccacc aaggaaggcc tgggcaagct caagtcggcc ttcgagggcc tggcggcgat gggcggtttc gacgacgtcg cgctgcagaa gtaccactgg gtggaaaaga tcaaccacgt gcacaccggc ggcaacagct ccggcatcgt cgatggcgcc gcgatcgtga tggtcggcag cgaaaaggcg ggggcagcga tcaacgcgac cccgcgcgcc cgcatcgtcg ccaccgcgac cagtggcgcc gacccggtca tcatgctgac cggcccgacc cctgccgccc gcaaggtgct cgaccgcgcc ggcctgacgg ttgatgacat cgatctgttc gagctgaacg aggcgttcgc gtcggtggtg ctgaagttcc agaaggacct caacatcccc gacgagaagc tcaacgtcaa cggtggggcc atcgcgatgg gtcacccgct gggggcgacc ggcgcgatga tcctgggcac catggtcgac gagctggagc gccgcaacgc ccggcgcgca ttggtcacgc tttgtattgg tggcggcatg ggtgccgcga cgatcatcga gagggttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa