MysmA.00640.a
Acetate kinase
| CENTER ID: | MysmA.00640.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00640.a.A1.PS00601 | Full length( MysmA.00640.a ) | 1 | 376 |
Structures
4IJN
DEPOSITED: 12/21/2012
DETERMINATION: XRay
CLONE: MysmA.00640.a.A1.GE28816
PROTEIN: MysmA.00640.a.A1.PS00601
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.779 |
| RefSeq: | YP_885189.1 |
| UniProt: | A0QQK1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVLVVNSGS SSLKYAVVRP ASGEFLADGI IEEIGSGAVP DHDAALRAAF DELAAAGLHL EDLDLKAVGH RMVHGGKTFY KPSVVDDELI AKARELSPLA PLHNPPAIKG IEVARKLLPD LPHIAVFDTA FFHDLPAPAS TYAIDRELAE TWHIKRYGFH GTSHEYVSQQ AAIFLDRPLE SLNQIVLHLG NGASASAVAG GKAVDTSMGL TPMEGLVMGT RSGDIDPGVI MYLWRTAGMS VDDIESMLNR RSGVLGLGGA SDFRKLRELI ESGDEHAKLA YDVYIHRLRK YIGAYMAVLG RTDVISFTAG VGENVPPVRR DALAGLGGLG IEIDDALNSA KSDEPRLIST PDSRVTVLVV PTNEELAIAR ACVGVV
NT Sequence
GTGACAGTAC TCGTCGTCAA CTCAGGGTCG TCGTCGCTGA AATACGCCGT GGTGCGGCCG GCCAGCGGGG AATTCCTGGC CGACGGCATC ATCGAGGAGA TCGGGTCGGG CGCGGTGCCC GACCATGACG CCGCGTTGCG CGCCGCGTTC GACGAACTCG CCGCCGCGGG TCTCCATCTG GAAGACCTCG ATCTGAAGGC CGTCGGGCAC CGGATGGTCC ACGGCGGCAA GACGTTCTAC AAGCCGTCGG TGGTCGACGA CGAGCTCATC GCCAAGGCGC GCGAGCTCTC CCCGCTGGCG CCGCTGCACA ACCCGCCCGC CATCAAGGGC ATCGAGGTCG CCCGCAAGCT GCTGCCGGAC CTGCCGCACA TCGCCGTGTT CGACACCGCG TTCTTCCACG ACCTGCCCGC GCCCGCGAGC ACCTACGCCA TCGACCGCGA ACTCGCCGAG ACGTGGCACA TCAAGCGCTA CGGTTTTCAT GGCACGTCGC ACGAGTACGT CAGCCAGCAG GCCGCGATCT TCCTCGACCG TCCGCTGGAA TCGCTGAATC AGATTGTGCT GCACCTGGGT AACGGTGCCT CGGCAAGCGC CGTGGCAGGT GGTAAGGCCG TCGACACGTC GATGGGTCTG ACCCCGATGG AGGGCCTGGT GATGGGCACG CGCAGCGGTG ACATCGACCC CGGGGTCATC ATGTACCTGT GGCGCACCGC GGGCATGAGC GTCGACGACA TCGAGTCGAT GCTCAACCGG CGCTCCGGGG TCCTCGGTCT CGGTGGCGCC AGCGACTTCC GCAAGCTTCG TGAGCTCATC GAATCCGGGG ATGAGCACGC GAAACTGGCG TACGACGTCT ACATCCACCG ACTGCGCAAG TACATCGGCG CGTACATGGC CGTGCTCGGC AGGACCGACG TGATCAGCTT CACCGCCGGG GTGGGCGAGA ACGTCCCCCC GGTGCGGCGC GATGCGTTGG CCGGGCTGGG CGGTCTGGGC ATCGAGATCG ATGATGCGCT GAACTCCGCG AAATCCGATG AGCCCCGGCT CATCTCGACC CCGGATTCGC GTGTGACCGT GCTCGTGGTG CCCACCAACG AGGAACTCGC GATCGCCCGT GCGTGTGTGG GCGTGGTG
Details for MysmA.00640.a.A1.PS00601
| PURIFICATION DATe: | 5/19/2010 |
| CONCENTRATION: | 50.33mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 93 |
| PERCENT COVERAGE: | 88 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTVLVVNS GSSSLKYAVV RPASGEFLAD GIIEEIGSGA VPDHDAALRA AFDELAAAGL HLEDLDLKAV GHRMVHGGKT FYKPSVVDDE LIAKARELSP LAPLHNPPAI KGIEVARKLL PDLPHIAVFD TAFFHDLPAP ASTYAIDREL AETWHIKRYG FHGTSHEYVS QQAAIFLDRP LESLNQIVLH LGNGASASAV AGGKAVDTSM GLTPMEGLVM GTRSGDIDPG VIMYLWRTAG MSVDDIESML NRRSGVLGLG GASDFRKLRE LIESGDEHAK LAYDVYIHXL RKYIGAYMXV LXRTDXXSFX XGXAXXPPGX XDAXXGWXXG XXXR
Validated NT Sequence
ttgtttactt taagaaggag anntaccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgacag tactcgtcgt caactcaggg tcgtcgtcgc tgaaatacgc cgtggtgcgg ccggccagcg gggaattcct ggccgacggc atcatcgagg agatcgggtc gggcgcggtg cccgaccatg acgccgcgtt gcgcgccgcg ttcgacgaac tcgccgccgc gggtctccat ctggaagacc tcgatctgaa ggccgtcggg caccggatgg tccacggcgg caagacgttc tacaagccgt cggtggtcga cgacgagctc atcgccaagg cgcgcgagct ctccccgctg gcgccgctgc acaacccgcc cgccatcaag ggcatcgagg tcgcccgcaa gctgctgccg gacctgccgc acatcgccgt gttcgacacc gcgttcttcc acgacctgcc cgcgcccgcg agcacctacg ccatcgaccg cgaactcgcc gagacgtggc acatcaagcg ctacggtttt catggcacgt cgcacgagta cgtcagccag caggccgcga tcttcctcga ccgtccgctg gaatcgctga atcagattgt gctgcacctg ggtaacggtg cctcggcaag cgccgtggca ggtggtaagg ccgtcgacac gtcgatgggt ctgaccccga tggagggcct ggtgatgggc acgcgcagcg gtgacatcga ccccggggtc atcatgtacc tgtggcgcac cgcgggcatg agcgtcgacg acatcgagtc gatgctcaac cggcgctccg gggtcctcgg tctcggtggc gccagcgact tccgcaagct tcgtgagctc atcgaatccg gggatgagca cgcgaaactg gcgtacgacg tctacatcca ccnactgcgc aagtacatcg gcgcgtacat gnnngtgctc ngcaggaccg acntgntcag cttcancnnn gggngggcga nangtccccc cggngngnnc gatgcnnnnn ngggntggnn nnctgggnat cnnnntcgat gatgcnntna nncnnnaann cnannnnccn nnnnatctcn accnnnntnn nnnnnnnacn nnnncnnnnn nnnnnnnnnn nnnnnnnnnn nnannnnaaa nnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTVLVVNS GSSSLKYAVV RPASGEFLAD GIIEEIGSGA VPDHDAALRA AFDELAAAGL HLEDLDLKAV GHRMVHGGKT FYKPSVVDDE LIAKARELSP LAPLHNPPAI KGIEVARKLL PDLPHIAVFD TAFFHDLPAP ASTYAIDREL AETWHIKRYG FHGTSHEYVS QQAAIFLDRP LESLNQIVLH LGNGASASAV AGGKAVDTSM GLTPMEGLVM GTRSGDIDPG VIMYLWRTAG MSVDDIESML NRRSGVLGLG GASDFRKLRE LIESGDEHAK LAYDVYIHRL RKYIGAYMAV LGRTDVISFT AGVGENVPPV RRDALAGLGG LGIEIDDALN SAKSDEPRLI STPDSRVTVL VVPTNEELAI ARACVGVV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACAGTA CTCGTCGTCA ACTCAGGGTC GTCGTCGCTG AAATACGCCG TGGTGCGGCC GGCCAGCGGG GAATTCCTGG CCGACGGCAT CATCGAGGAG ATCGGGTCGG GCGCGGTGCC CGACCATGAC GCCGCGTTGC GCGCCGCGTT CGACGAACTC GCCGCCGCGG GTCTCCATCT GGAAGACCTC GATCTGAAGG CCGTCGGGCA CCGGATGGTC CACGGCGGCA AGACGTTCTA CAAGCCGTCG GTGGTCGACG ACGAGCTCAT CGCCAAGGCG CGCGAGCTCT CCCCGCTGGC GCCGCTGCAC AACCCGCCCG CCATCAAGGG CATCGAGGTC GCCCGCAAGC TGCTGCCGGA CCTGCCGCAC ATCGCCGTGT TCGACACCGC GTTCTTCCAC GACCTGCCCG CGCCCGCGAG CACCTACGCC ATCGACCGCG AACTCGCCGA GACGTGGCAC ATCAAGCGCT ACGGTTTTCA TGGCACGTCG CACGAGTACG TCAGCCAGCA GGCCGCGATC TTCCTCGACC GTCCGCTGGA ATCGCTGAAT CAGATTGTGC TGCACCTGGG TAACGGTGCC TCGGCAAGCG CCGTGGCAGG TGGTAAGGCC GTCGACACGT CGATGGGTCT GACCCCGATG GAGGGCCTGG TGATGGGCAC GCGCAGCGGT GACATCGACC CCGGGGTCAT CATGTACCTG TGGCGCACCG CGGGCATGAG CGTCGACGAC ATCGAGTCGA TGCTCAACCG GCGCTCCGGG GTCCTCGGTC TCGGTGGCGC CAGCGACTTC CGCAAGCTTC GTGAGCTCAT CGAATCCGGG GATGAGCACG CGAAACTGGC GTACGACGTC TACATCCACC GACTGCGCAA GTACATCGGC GCGTACATGG CCGTGCTCGG CAGGACCGAC GTGATCAGCT TCACCGCCGG GGTGGGCGAG AACGTCCCCC CGGTGCGGCG CGATGCGTTG GCCGGGCTGG GCGGTCTGGG CATCGAGATC GATGATGCGC TGAACTCCGC GAAATCCGAT GAGCCCCGGC TCATCTCGAC CCCGGATTCG CGTGTGACCG TGCTCGTGGT GCCCACCAAC GAGGAACTCG CGATCGCCCG TGCGTGTGTG GGCGTGGTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A