MyabA.00640.a

Acetate kinase (AckA)

CENTER ID: MyabA.00640.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00640.a.A1.GE34908 Full length( MyabA.00640.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00640.a.A1.PW35523 Full length( MyabA.00640.a ) 1 372

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4304
RefSeq: YP_001704952.1
UniProt: B1MIS7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAGGSVLVL NCGSSSVKYQ LIEPESGRVI AHGLVERIGE EEIGNHEEAL RLVFDSIDTG DVVVVGHRVV HGGQKFHEPT VLDDAVVAQI AELSSLAPLH NPAGVQGIEV ARRLLPDVPQ VAVFDTAFFF GLPPAAAIYA LDRAVAERYG IRRYGFHGTS HQYVSQQAAA FLGRGYADIN QIVLHLGNGA SASAILRGRA VETSMGLTPL EGLVMGTRTG DIDAGIVFHL ARHGMSIDEI DTVFNRRSGM LGLCGANDFR EVHRLIDAGD AAAQLAYNVY VHRLRKYIGA YIATLGGADV ISFTAGAGEN DALLRADALA GLEVFGIEID AARNQVRSGE ARRISTDSSR VTVLVVPTNE ELAIARAAVE AI
NT Sequence
GTGAGCGCCG GCGGGTCTGT TCTGGTCCTC AACTGCGGCT CGTCCTCGGT CAAGTATCAG CTCATCGAGC CGGAATCGGG CCGAGTGATT GCTCACGGAC TGGTGGAGCG CATCGGCGAG GAGGAGATCG GCAACCACGA AGAGGCGTTG CGGCTGGTCT TCGACTCCAT CGACACCGGT GATGTGGTGG TAGTCGGTCA CCGTGTAGTG CACGGGGGCC AGAAGTTTCA CGAACCGACG GTGCTTGACG ACGCGGTGGT CGCGCAGATC GCCGAGCTGT CTTCCCTGGC ACCGCTACAC AACCCGGCCG GGGTACAGGG CATCGAGGTG GCGCGGCGCC TGCTCCCGGA TGTGCCGCAG GTGGCGGTTT TCGACACCGC GTTCTTCTTC GGCCTGCCAC CGGCGGCGGC CATCTACGCG CTGGATCGTG CTGTCGCAGA GCGATATGGC ATCCGTCGCT ACGGGTTTCA CGGTACCTCG CACCAGTATG TGTCGCAGCA GGCCGCGGCC TTCCTGGGGC GTGGGTATGC CGACATCAAC CAGATCGTGC TGCATCTGGG CAACGGGGCG TCGGCGTCGG CGATTCTGCG CGGCCGCGCG GTGGAAACCT CGATGGGCCT GACCCCGCTG GAGGGGTTGG TGATGGGCAC CCGCACCGGT GATATCGACG CAGGCATCGT GTTCCACCTG GCCCGTCACG GCATGAGCAT CGACGAGATC GACACGGTGT TCAACAGACG GTCGGGCATG CTTGGGTTGT GTGGTGCCAA TGACTTTCGT GAGGTGCACC GTTTGATCGA TGCCGGTGAT GCCGCGGCCC AGCTTGCCTA CAACGTGTAT GTGCACCGGT TGCGTAAGTA CATCGGTGCC TACATCGCGA CATTGGGTGG CGCCGACGTC ATTTCGTTCA CCGCCGGGGC AGGGGAGAAC GACGCGTTGC TGCGGGCCGA TGCCCTGGCG GGGCTGGAAG TGTTCGGTAT CGAGATCGAC GCCGCGCGTA ACCAGGTGCG GTCCGGTGAG GCTCGCCGCA TCTCCACCGA CTCCTCACGG GTGACGGTAC TCGTGGTGCC CACCAATGAG GAGCTGGCGA TCGCGCGTGC CGCGGTGGAA GCGATC
Details for MyabA.00640.a.A1.GE34908
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAGGSVL VLNCGSSSVK YQLIEPESGR VIAHGLVERI GEEEIGNHEE ALRLVFDSID TGDVVVVGHR VVHGGQKFHE PTVLDDAVVA QIAELSSLAP LHNPAGVQGI EVARRLLPDV PQVAVFDTAF FFGLPPAAAI YALDRAVAER YGIRRYGFHG TSHQYVSQQA AAFLGRGYAD INQIVLHLGN GASASAILRG RAVETSMGLT PLEGLVMGTR TGDIDAGIVF HLARHGMSID EIDTVFNRRS GMLGLCGAND FREVHRLIDA GDAAAQLAYN VYVHRLRKYI GAYIATLGGA DVISFTAGAG ENDALLRADA LAGLEVFGIE IDAARNQVRS GEARRISTDS SRVTVLVVPT NEELAI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgccggcgg gtctgttctg gtcctcaact gcggctcgtc ctcggtcaag tatcagctca tcgagccgga atcgggccga gtgattgctc acggactggt ggagcgcatc ggcgaggagg agatcggcaa ccacgaagag gcgttgcggc tggtcttcga ctccatcgac accggtgatg tggtggtagt cggtcaccgt gtagtgcacg ggggccagaa gtttcacgaa ccgacggtgc ttgacgacgc ggtggtcgcg cagatcgccg agctgtcttc cctggcaccg ctacacaacc cggccggggt acagggcatc gaggtggcgc ggcgcctgct cccggatgtg ccgcaggtgg cggttttcga caccgcgttc ttcttcggcc tgccaccggc ggcggccatc tacgcgctgg atcgtgctgt cgcagagcga tatggcatcc gtcgctacgg gtttcacggt acctcgcacc agtatgtgtc gcagcaggcc gcggccttcc tggggcgtgg gtatgccgac atcaaccaga tcgtgctgca tctgggcaac ggggcgtcgg cgtcggcgat tctgcgcggc cgcgcggtgg aaacctcgat gggcctgacc ccgctggagg ggttggtgat gggcacccgc accggtgata tcgacgcagg catcgtgttc cacctggccc gtcacggcat gagcatcgac gagatcgaca cggtgttcaa cagacggtcg ggcatgcttg ggttgtgtgg tgccaatgac tttcgtgagg tgcaccgttt gatcgatgcc ggtgatgccg cggcccagct tgcctacaac gtgtatgtgc accggttgcg taagtacatc ggtgcntaca tcgcgacatt gggtggcgcc gacgtcattt cgttcaccgc cggggcaggg gagaacgacg cgttgctgcg ggccgatgcc ctggcggggc tggaagtgtt cggtatcgag atcgacgccg cgcgtaacca ggtgcggtcc ggtgaggctc gccgcatctc caccgactcc tcacgggtga cggtactcgt ggtgcccacc aatgaggagc tggcgatcgc g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAGGSVL VLNCGSSSVK YQLIEPESGR VIAHGLVERI GEEEIGNHEE ALRLVFDSID TGDVVVVGHR VVHGGQKFHE PTVLDDAVVA QIAELSSLAP LHNPAGVQGI EVARRLLPDV PQVAVFDTAF FFGLPPAAAI YALDRAVAER YGIRRYGFHG TSHQYVSQQA AAFLGRGYAD INQIVLHLGN GASASAILRG RAVETSMGLT PLEGLVMGTR TGDIDAGIVF HLARHGMSID EIDTVFNRRS GMLGLCGAND FREVHRLIDA GDAAAQLAYN VYVHRLRKYI GAYIATLGGA DVISFTAGAG ENDALLRADA LAGLEVFGIE IDAARNQVRS GEARRISTDS SRVTVLVVPT NEELAIARAA VEAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgcc ggcgggtctg ttctggtcct caactgcggc tcgtcctcgg tcaagtatca gctcatcgag ccggaatcgg gccgagtgat tgctcacgga ctggtggagc gcatcggcga ggaggagatc ggcaaccacg aagaggcgtt gcggctggtc ttcgactcca tcgacaccgg tgatgtggtg gtagtcggtc accgtgtagt gcacgggggc cagaagtttc acgaaccgac ggtgcttgac gacgcggtgg tcgcgcagat cgccgagctg tcttccctgg caccgctaca caacccggcc ggggtacagg gcatcgaggt ggcgcggcgc ctgctcccgg atgtgccgca ggtggcggtt ttcgacaccg cgttcttctt cggcctgcca ccggcggcgg ccatctacgc gctggatcgt gctgtcgcag agcgatatgg catccgtcgc tacgggtttc acggtacctc gcaccagtat gtgtcgcagc aggccgcggc cttcctgggg cgtgggtatg ccgacatcaa ccagatcgtg ctgcatctgg gcaacggggc gtcggcgtcg gcgattctgc gcggccgcgc ggtggaaacc tcgatgggcc tgaccccgct ggaggggttg gtgatgggca cccgcaccgg tgatatcgac gcaggcatcg tgttccacct ggcccgtcac ggcatgagca tcgacgagat cgacacggtg ttcaacagac ggtcgggcat gcttgggttg tgtggtgcca atgactttcg tgaggtgcac cgtttgatcg atgccggtga tgccgcggcc cagcttgcct acaacgtgta tgtgcaccgg ttgcgtaagt acatcggtgc ctacatcgcg acattgggtg gcgccgacgt catttcgttc accgccgggg caggggagaa cgacgcgttg ctgcgggccg atgccctggc ggggctggaa gtgttcggta tcgagatcga cgccgcgcgt aaccaggtgc ggtccggtga ggctcgccgc atctccaccg actcctcacg ggtgacggta ctcgtggtgc ccaccaatga ggagctggcg atcgcgcgtg ccgcggtgga agcgatctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyabA.00640.a.A1.PW35523
PURIFICATION DATe: 6/14/2012
CONCENTRATION: 16.86mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 27
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAGGSVL VLNCGSSSVK YQLIEPESGR VIAHGLVERI GEEEIGNHEE ALRLVFDSID TGDVVVVGHR VVHGGQKFHE PTVLDDAVVA QIAELSSLAP LHNPAGVQGI EVARRLLPDV PQVAVFDTAF FFGLPPAAAI YALDRAVAER YGIRRYGFHG TSHQYVSQQA AAFLGRGYAD INQIVLHLGN GASASAILRG RAVETSMGLT PLEGLVMGTR TGDIDAGIVF HLARHGMSID EIDTVFNRRS GMLGLCGAND FREVHRLIDA GDAAAQLAYN VYVHRLRKYI GAYIATLGGA DVISFTAGAG ENDALLRADA LAGLEVFGIE IDAARNQVRS GEARRISTDS SRVTVLVVPT NEELAI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgccggcgg gtctgttctg gtcctcaact gcggctcgtc ctcggtcaag tatcagctca tcgagccgga atcgggccga gtgattgctc acggactggt ggagcgcatc ggcgaggagg agatcggcaa ccacgaagag gcgttgcggc tggtcttcga ctccatcgac accggtgatg tggtggtagt cggtcaccgt gtagtgcacg ggggccagaa gtttcacgaa ccgacggtgc ttgacgacgc ggtggtcgcg cagatcgccg agctgtcttc cctggcaccg ctacacaacc cggccggggt acagggcatc gaggtggcgc ggcgcctgct cccggatgtg ccgcaggtgg cggttttcga caccgcgttc ttcttcggcc tgccaccggc ggcggccatc tacgcgctgg atcgtgctgt cgcagagcga tatggcatcc gtcgctacgg gtttcacggt acctcgcacc agtatgtgtc gcagcaggcc gcggccttcc tggggcgtgg gtatgccgac atcaaccaga tcgtgctgca tctgggcaac ggggcgtcgg cgtcggcgat tctgcgcggc cgcgcggtgg aaacctcgat gggcctgacc ccgctggagg ggttggtgat gggcacccgc accggtgata tcgacgcagg catcgtgttc cacctggccc gtcacggcat gagcatcgac gagatcgaca cggtgttcaa cagacggtcg ggcatgcttg ggttgtgtgg tgccaatgac tttcgtgagg tgcaccgttt gatcgatgcc ggtgatgccg cggcccagct tgcctacaac gtgtatgtgc accggttgcg taagtacatc ggtgcntaca tcgcgacatt gggtggcgcc gacgtcattt cgttcaccgc cggggcaggg gagaacgacg cgttgctgcg ggccgatgcc ctggcggggc tggaagtgtt cggtatcgag atcgacgccg cgcgtaacca ggtgcggtcc ggtgaggctc gccgcatctc caccgactcc tcacgggtga cggtactcgt ggtgcccacc aatgaggagc tggcgatcgc g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAGGSVL VLNCGSSSVK YQLIEPESGR VIAHGLVERI GEEEIGNHEE ALRLVFDSID TGDVVVVGHR VVHGGQKFHE PTVLDDAVVA QIAELSSLAP LHNPAGVQGI EVARRLLPDV PQVAVFDTAF FFGLPPAAAI YALDRAVAER YGIRRYGFHG TSHQYVSQQA AAFLGRGYAD INQIVLHLGN GASASAILRG RAVETSMGLT PLEGLVMGTR TGDIDAGIVF HLARHGMSID EIDTVFNRRS GMLGLCGAND FREVHRLIDA GDAAAQLAYN VYVHRLRKYI GAYIATLGGA DVISFTAGAG ENDALLRADA LAGLEVFGIE IDAARNQVRS GEARRISTDS SRVTVLVVPT NEELAIARAA VEAI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCC GGCGGGTCTG TTCTGGTCCT CAACTGCGGC TCGTCCTCGG TCAAGTATCA GCTCATCGAG CCGGAATCGG GCCGAGTGAT TGCTCACGGA CTGGTGGAGC GCATCGGCGA GGAGGAGATC GGCAACCACG AAGAGGCGTT GCGGCTGGTC TTCGACTCCA TCGACACCGG TGATGTGGTG GTAGTCGGTC ACCGTGTAGT GCACGGGGGC CAGAAGTTTC ACGAACCGAC GGTGCTTGAC GACGCGGTGG TCGCGCAGAT CGCCGAGCTG TCTTCCCTGG CACCGCTACA CAACCCGGCC GGGGTACAGG GCATCGAGGT GGCGCGGCGC CTGCTCCCGG ATGTGCCGCA GGTGGCGGTT TTCGACACCG CGTTCTTCTT CGGCCTGCCA CCGGCGGCGG CCATCTACGC GCTGGATCGT GCTGTCGCAG AGCGATATGG CATCCGTCGC TACGGGTTTC ACGGTACCTC GCACCAGTAT GTGTCGCAGC AGGCCGCGGC CTTCCTGGGG CGTGGGTATG CCGACATCAA CCAGATCGTG CTGCATCTGG GCAACGGGGC GTCGGCGTCG GCGATTCTGC GCGGCCGCGC GGTGGAAACC TCGATGGGCC TGACCCCGCT GGAGGGGTTG GTGATGGGCA CCCGCACCGG TGATATCGAC GCAGGCATCG TGTTCCACCT GGCCCGTCAC GGCATGAGCA TCGACGAGAT CGACACGGTG TTCAACAGAC GGTCGGGCAT GCTTGGGTTG TGTGGTGCCA ATGACTTTCG TGAGGTGCAC CGTTTGATCG ATGCCGGTGA TGCCGCGGCC CAGCTTGCCT ACAACGTGTA TGTGCACCGG TTGCGTAAGT ACATCGGTGC CTACATCGCG ACATTGGGTG GCGCCGACGT CATTTCGTTC ACCGCCGGGG CAGGGGAGAA CGACGCGTTG CTGCGGGCCG ATGCCCTGGC GGGGCTGGAA GTGTTCGGTA TCGAGATCGA CGCCGCGCGT AACCAGGTGC GGTCCGGTGA GGCTCGCCGC ATCTCCACCG ACTCCTCACG GGTGACGGTA CTCGTGGTGC CCACCAATGA GGAGCTGGCG ATCGCGCGTG CCGCGGTGGA AGCGATCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA