MyavA.00640.a
Acetate kinase
| CENTER ID: | MyavA.00640.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00640.a.A1.GE28563 | Full length( MyavA.00640.a ) | 1 | 387 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3P4I
DEPOSITED: 10/6/2010
DETERMINATION: XRay
CLONE: MyavA.00640.a.A1.GE28563
PROTEIN: MyavA.00640.a.A1.PS00663
4IZ9
DEPOSITED: 1/29/2013
DETERMINATION: XRay
CLONE: MyavA.00640.a.A1.GE28563
PROTEIN: MyavA.00640.a.A1.PS00663
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130287 |
| PATRIC ID: | fig|243243.7.peg.4678 |
| RefSeq: | YP_883886.1 |
| UniProt: | A0QLU8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGSDGARRV LVINSGSSSL KFQLVDPESG VAASTGIVER IGEESSPVPD HDAALRRAFD MLAGDGVDLN TAGLVAVGHR VVHGGNTFYR PTVLDDAVIA RLHELSELAP LHNPPALLGI EVARRLLPGI AHVAVFDTGF FHDLPPAAAT YAIDRELADR WQIRRYGFHG TSHRYVSEQA AAFLDRPLRG LKQIVLHLGN GCSASAIAGT RPLDTSMGLT PLEGLVMGTR SGDIDPSVVS YLCHTAGMGV DDVESMLNHR SGVVGLSGVR DFRRLRELIE SGDGAAQLAY SVFTHRLRKY IGAYLAVLGH TDVISFTAGI GENDAAVRRD AVSGMEELGI VLDERRNLPG AKGARQISAD DSPITVLVVP TNEELAIARD CVRVLGG
NT Sequence
GTGGACGGTA GCGACGGCGC GCGCCGGGTG CTGGTGATCA ACTCCGGCTC GTCGTCGCTG AAGTTCCAGC TGGTCGATCC CGAGTCCGGG GTGGCCGCCT CGACGGGCAT CGTGGAACGC ATCGGCGAGG AATCCTCGCC GGTCCCCGAC CACGACGCCG CGCTGCGCCG AGCGTTCGAC ATGCTGGCCG GCGACGGCGT CGACCTGAAC ACCGCCGGGC TGGTCGCGGT GGGTCATCGG GTGGTGCACG GCGGCAACAC CTTCTACCGG CCCACCGTGC TGGACGACGC GGTGATCGCC CGGCTGCACG AGCTGTCGGA GTTGGCCCCG CTGCACAATC CGCCGGCGCT CCTGGGGATC GAAGTGGCCC GCAGGCTGCT GCCCGGCATC GCGCACGTGG CCGTCTTCGA CACCGGGTTC TTCCACGACC TGCCGCCCGC GGCGGCGACC TACGCCATCG ACCGCGAACT GGCCGACCGC TGGCAGATCC GCCGGTACGG GTTCCACGGC ACCTCGCACC GCTACGTCAG CGAGCAGGCC GCGGCTTTCC TGGATCGGCC GCTGCGCGGC CTGAAACAGA TCGTGCTGCA CCTGGGCAAC GGCTGCTCGG CGTCAGCGAT CGCCGGCACC CGGCCCCTCG ACACCTCGAT GGGGTTGACG CCGCTGGAGG GACTGGTGAT GGGTACCCGC AGCGGTGACA TCGATCCCAG CGTCGTCAGC TATCTGTGCC ACACGGCCGG GATGGGGGTG GACGACGTCG AGTCGATGCT CAACCACCGC TCGGGGGTGG TCGGGCTGTC CGGCGTGCGG GACTTCCGCC GGCTGCGCGA ACTCATCGAA TCCGGGGACG GCGCAGCGCA ATTGGCCTAC AGCGTGTTCA CGCACCGGCT GCGCAAGTAC ATCGGTGCCT ACCTGGCGGT GCTGGGCCAC ACCGACGTCA TCAGCTTCAC CGCCGGCATC GGTGAGAACG ACGCCGCGGT GCGCCGCGAC GCGGTGTCGG GCATGGAGGA GCTGGGCATT GTGCTCGACG AGCGCCGCAA CCTGCCCGGG GCCAAAGGGG CACGCCAGAT CTCGGCCGAC GACTCGCCGA TCACGGTGCT GGTGGTCCCC ACCAACGAAG AGCTGGCCAT CGCCCGTGAC TGCGTGCGGG TGCTAGGCGG A
Details for MyavA.00640.a.A1.GE28563
| HARVESTED ON: | 2/9/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDGSDGAR RVLVINSGSS SLKFQLVDPE SGVAASTGIV ERIGEESSPV PDHDAALRRA FDMLAGDGVD LNTAGLVAVG HRVVHGGNTF YRPTVLDDAV IARLHELSEL APLHNPPALL GIEVARRLLP GIAHVAVFDT GFFHDLPPAA ATYAIDRELA DRWQIRRYGF HGTSHRYVSE QAAAFLDRPL RGLKQIVLHL GNGCSASAIA GTRPLDTSMG LTPLEGLVMG TRSGDIDPSV VSYLCHTAGM GVDDVESMLN HRSGVVGLSG VRDFRRLREL IESGDGAAQL AYSVFTHRLR KYIGAYLAVL GHTDVISFTA GIGENDAAVR RDAVSGMEEL GIVLDERRNL PGAKGARQIS ADDSPITVLV VPTNEELAIA RDCVRVLGG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg acggtagcga cggcgcgcgc cgggtgctgg tgatcaactc cggctcgtcg tcgctgaagt tccagctggt cgatcccgag tccggggtgg ccgcctcgac gggcatcgtg gaacgcatcg gcgaggaatc ctcgccggtc cccgaccacg acgccgcgct gcgccgagcg ttcgacatgc tggccggcga cggcgtcgac ctgaacaccg ccgggctggt cgcggtgggt catcgggtgg tgcacggcgg caacaccttc taccggccca ccgtgctgga cgacgcggtg atcgcccggc tgcacgagct gtcggagttg gccccgctgc acaatccgcc ggcgctcctg gggatcgaag tggcccgcag gctgctgccc ggcatcgcgc acgtggccgt cttcgacacc gggttcttcc acgacctgcc gcccgcggcg gcgacctacg ccatcgaccg cgaactggcc gaccgctggc agatccgccg gtacgggttc cacggcacct cgcaccgcta cgtcagcgag caggccgcgg ctttcctgga tcggccgctg cgcggcctga aacagatcgt gctgcacctg ggcaacggct gctcggcgtc agcgatcgcc ggcacccggc ccctcgacac ctcgatgggg ttgacgccgc tggagggact ggtgatgggt acccgcagcg gtgacatcga tcccagcgtc gtcagctatc tgtgccacac ggccgggatg ggggtggacg acgtcgagtc gatgctcaac caccgctcgg gggtggtcgg gctgtccggc gtgcgggact tccgccggct gcgcgaactc atcgaatccg gggacggcgc agcgcaattg gcctacagcg tgttcacgca ccggctgcgc aagtacatcg gtgcctacct ggcggtgctg ggccacaccg acgtcatcag cttcaccgcc ggcatcggtg agaacgacgc cgcggtgcgc cgcgacgcgg tgtcgggcat ggaggagctg ggcattgtgc tcgacgagcg ccgcaacctg cccggggcca aaggggcacg ccagatctcg gccgacgact cgccgatcac ggtgctggtg gtccccacca acgaagagct ggccatcgcc cgtgactgcg tgcgggtgct aggcggataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDGSDGAR RVLVINSGSS SLKFQLVDPE SGVAASTGIV ERIGEESSPV PDHDAALRRA FDMLAGDGVD LNTAGLVAVG HRVVHGGNTF YRPTVLDDAV IARLHELSEL APLHNPPALL GIEVARRLLP GIAHVAVFDT GFFHDLPPAA ATYAIDRELA DRWQIRRYGF HGTSHRYVSE QAAAFLDRPL RGLKQIVLHL GNGCSASAIA GTRPLDTSMG LTPLEGLVMG TRSGDIDPSV VSYLCHTAGM GVDDVESMLN HRSGVVGLSG VRDFRRLREL IESGDGAAQL AYSVFTHRLR KYIGAYLAVL GHTDVISFTA GIGENDAAVR RDAVSGMEEL GIVLDERRNL PGAKGARQIS ADDSPITVLV VPTNEELAIA RDCVRVLGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacggt agcgacggcg cgcgccgggt gctggtgatc aactccggct cgtcgtcgct gaagttccag ctggtcgatc ccgagtccgg ggtggccgcc tcgacgggca tcgtggaacg catcggcgag gaatcctcgc cggtccccga ccacgacgcc gcgctgcgcc gagcgttcga catgctggcc ggcgacggcg tcgacctgaa caccgccggg ctggtcgcgg tgggtcatcg ggtggtgcac ggcggcaaca ccttctaccg gcccaccgtg ctggacgacg cggtgatcgc ccggctgcac gagctgtcgg agttggcccc gctgcacaat ccgccggcgc tcctggggat cgaagtggcc cgcaggctgc tgcccggcat cgcgcacgtg gccgtcttcg acaccgggtt cttccacgac ctgccgcccg cggcggcgac ctacgccatc gaccgcgaac tggccgaccg ctggcagatc cgccggtacg ggttccacgg cacctcgcac cgctacgtca gcgagcaggc cgcggctttc ctggatcggc cgctgcgcgg cctgaaacag atcgtgctgc acctgggcaa cggctgctcg gcgtcagcga tcgccggcac ccggcccctc gacacctcga tggggttgac gccgctggag ggactggtga tgggtacccg cagcggtgac atcgatccca gcgtcgtcag ctatctgtgc cacacggccg ggatgggggt ggacgacgtc gagtcgatgc tcaaccaccg ctcgggggtg gtcgggctgt ccggcgtgcg ggacttccgc cggctgcgcg aactcatcga atccggggac ggcgcagcgc aattggccta cagcgtgttc acgcaccggc tgcgcaagta catcggtgcc tacctggcgg tgctgggcca caccgacgtc atcagcttca ccgccggcat cggtgagaac gacgccgcgg tgcgccgcga cgcggtgtcg ggcatggagg agctgggcat tgtgctcgac gagcgccgca acctgcccgg ggccaaaggg gcacgccaga tctcggccga cgactcgccg atcacggtgc tggtggtccc caccaacgaa gagctggcca tcgcccgtga ctgcgtgcgg gtgctaggcg gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa