MypaA.00640.a
Acetate kinase (EC=2.7.2.1) (AckA)
| CENTER ID: | MypaA.00640.a |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00640.a.A1.GE29470 | Full length( MypaA.00640.a ) | 1 | 387 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3R9P
DEPOSITED: 3/25/2011
DETERMINATION: XRay
CLONE: MypaA.00640.a.A1.GE29470
PROTEIN: MypaA.00640.a.A1.PS00722
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130287 |
| PATRIC ID: | fig|262316.17.peg.4137 |
| RefSeq: | NP_962820.1 |
| UniProt: | Q73T33 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGSDGARRV LVINSGSSSL KFQLVDPEFG VAASTGIVER IGEESSPVPD HDAALRRAFD MLAGDGVDLN TAGLVAVGHR VVHGGNTFYR PTVLDDAVIA RLHELSELAP LHNPPALQGI EVARRLLPDI AHVAVFDTGF FHDLPPAAAT YAIDRELADR WQIRRYGFHG TSHRYVSEQA AAFLDRPLRG LKQIVLHLGN GCSASAIAGT RPLDTSMGLT PLEGLVMGTR SGDIDPSIVS YLCHTAGMGV DDVESMLNHR SGVVGLSGVR DFRRLRELIE SGDGAAQLAY SVFTHRLRKY IGAYLAVLGH TDVISFTAGI GENDAAVRRD AVSGMEELGI VLDERRNLAG GKGARQISAD DSPITVLVVP TNEELAIARD CVRVLGG
NT Sequence
GTGGACGGTA GCGACGGCGC GCGCCGGGTG CTGGTGATCA ACTCCGGCTC GTCGTCGCTG AAGTTCCAGC TGGTCGATCC CGAGTTCGGG GTGGCCGCCT CGACGGGCAT CGTGGAACGC ATCGGCGAGG AATCCTCGCC GGTCCCCGAC CACGACGCCG CGCTGCGCCG AGCGTTCGAC ATGCTGGCCG GCGACGGCGT CGACCTGAAC ACCGCCGGGC TGGTCGCGGT GGGTCATCGG GTGGTGCACG GCGGCAACAC CTTCTACCGG CCCACCGTGC TGGACGACGC GGTGATCGCC CGGCTGCACG AGCTGTCGGA GTTGGCCCCG CTGCACAATC CGCCGGCGCT CCAGGGAATC GAAGTGGCCC GCCGGCTGTT GCCCGACATC GCGCACGTGG CCGTCTTCGA CACCGGGTTC TTCCACGACC TGCCGCCCGC GGCGGCGACC TACGCCATCG ACCGCGAATT GGCCGATCGC TGGCAGATCC GCCGGTACGG GTTCCACGGC ACCTCGCACC GCTACGTCAG CGAGCAGGCC GCGGCCTTCC TGGATCGGCC GCTGCGCGGC CTGAAACAGA TCGTGCTGCA CCTGGGCAAC GGCTGCTCGG CGTCGGCGAT CGCCGGCACC CGGCCCCTCG ACACCTCGAT GGGGTTGACG CCGCTGGAGG GACTGGTGAT GGGAACCCGC AGCGGTGACA TCGATCCCAG CATCGTCAGC TATCTGTGCC ACACGGCCGG GATGGGGGTG GACGACGTCG AGTCGATGCT CAACCACCGC TCGGGGGTGG TGGGGCTGTC CGGCGTGCGG GACTTCCGCC GGCTGCGCGA ACTCATCGAA TCCGGTGACG GCGCAGCGCA ATTGGCCTAC AGCGTGTTCA CGCACCGGCT GCGCAAGTAC ATCGGAGCCT ACCTGGCGGT GCTGGGCCAC ACCGACGTCA TCAGCTTCAC CGCCGGCATC GGTGAGAACG ACGCCGCGGT GCGCCGCGAC GCGGTGTCCG GCATGGAGGA GCTGGGCATC GTGCTCGACG AGCGCCGCAA CCTCGCCGGG GGCAAAGGGG CACGCCAGAT CTCGGCCGAC GACTCGCCGA TCACGGTGCT GGTGGTCCCC ACCAACGAAG AGCTGGCCAT CGCCCGTGAC TGCGTGCGGG TGCTAGGCGG A
Details for MypaA.00640.a.A1.GE29470
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVDGSDG ARRVLVINSG SSSLKFQLVD PEFGVAASTG IVERIGEESS PVPDHDAALR RAFDMLAGDG VDLNTAGLVA VGHRVVHGGN TFYRPTVLDD AVIARLHELS ELAPLHNPPA LQGIEVARRL LPDIAHVAVF DTGFFHDLPP AAATYAIDRE LADRWQIRRY GFHGTSHRYV SEQAAAFLDR PLRGLKQIVL HLGNGCSASA IAGTRPLDTS MGLTPLEGLV MGTRSGDIDP SIVSYLCHTA GMGVDDVESM LNHRSGVVGL SGVRDFRRLR ELIESGDGAA QLAYSVFTHR LRKYIGAYLA VLGHTDVISF TAGIGENDAA VRRDAVSGME ELGIVLDERR NLAGGKGARQ ISADDSPITV LVVPTNEELA IARDCVRVLG
Validated NT Sequence
ccgcctagca cccgcacgca gtcacgggcg atggccagct cttcgttggt ggggaccacc agcaccgtga tcggcgagtc gtcggccgag atctggcgtg cccctttgcc cccggcgagg ttgcggcgct cgtcgagcac gatgcccagc tcctccatgc cggacaccgc gtcgcggcgc accgcggcgt cgttctcacc gatgccggcg gtgaagctga tgacgtcggt gtggcccagc accgccaggt aggctccgat gtacttgcgc agccggtgcg tgaacacgct gtaggccaat tgcgctgcgc cgtcaccgga ttcgatgagt tcgcgcagcc ggcggaagtc ccgcacgccg gacagcccca ccacccccga gcggtggttg agcatcgact cgacgtcgtc cacccccatc ccggccgtgt ggcacagata gctgacgatg ctgggatcga tgtcaccgct gcgggttccc atcaccagtc cctccagcgg cgtcaacccc atcgaggtgt cgaggggccg ggtgccggcg atcgccgacg ccgagcagcc gttgcccagg tgcagcacga tctgtttcag gccgcgcagc ggccgatcca ggaaggccgc ggcctgctcg ctgacgtagc ggtgcgaggt gccgtggaac ccgtaccggc ggatctgcca gcgatcggcc aattcgcggt cgatggcgta ggtcgccgcc gcgggcggca ggtcgtggaa gaacccggtg tcgaagacgg ccacgtgcgc gatgtcgggc aacagccggc gggccacttc gattccctgg agcgccggcg gattgtgcag cggggccaac tccgacagct cgtgcagccg ggcgatcacc gcgtcgtcca gcacggtggg ccggtagaag gtgttgccgc cgtgcaccac ccgatgaccc accgcgacca gcccggcggt gttcaggtcg acgccgtcgc cggccagcat gtcgaacgct cggcgcagcg cggcgtcgtg gtcggggacc ggcgaggatt cctcgccgat gcgttccacg atgcccgtcg aggcggccac cccgaactcg ggatcgacca gctggaactt cagcgacgac gagccggagt tgatcaccag cacccggcgc gcgccgtcgc taccgtccac catcgaacca ggaccctggg tctgagcttc cagggtaccc ata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDGSDGAR RVLVINSGSS SLKFQLVDPE FGVAASTGIV ERIGEESSPV PDHDAALRRA FDMLAGDGVD LNTAGLVAVG HRVVHGGNTF YRPTVLDDAV IARLHELSEL APLHNPPALQ GIEVARRLLP DIAHVAVFDT GFFHDLPPAA ATYAIDRELA DRWQIRRYGF HGTSHRYVSE QAAAFLDRPL RGLKQIVLHL GNGCSASAIA GTRPLDTSMG LTPLEGLVMG TRSGDIDPSI VSYLCHTAGM GVDDVESMLN HRSGVVGLSG VRDFRRLREL IESGDGAAQL AYSVFTHRLR KYIGAYLAVL GHTDVISFTA GIGENDAAVR RDAVSGMEEL GIVLDERRNL AGGKGARQIS ADDSPITVLV VPTNEELAIA RDCVRVLGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacggt agcgacggcg cgcgccgggt gctggtgatc aactccggct cgtcgtcgct gaagttccag ctggtcgatc ccgagttcgg ggtggccgcc tcgacgggca tcgtggaacg catcggcgag gaatcctcgc cggtccccga ccacgacgcc gcgctgcgcc gagcgttcga catgctggcc ggcgacggcg tcgacctgaa caccgccggg ctggtcgcgg tgggtcatcg ggtggtgcac ggcggcaaca ccttctaccg gcccaccgtg ctggacgacg cggtgatcgc ccggctgcac gagctgtcgg agttggcccc gctgcacaat ccgccggcgc tccagggaat cgaagtggcc cgccggctgt tgcccgacat cgcgcacgtg gccgtcttcg acaccgggtt cttccacgac ctgccgcccg cggcggcgac ctacgccatc gaccgcgaat tggccgatcg ctggcagatc cgccggtacg ggttccacgg cacctcgcac cgctacgtca gcgagcaggc cgcggccttc ctggatcggc cgctgcgcgg cctgaaacag atcgtgctgc acctgggcaa cggctgctcg gcgtcggcga tcgccggcac ccggcccctc gacacctcga tggggttgac gccgctggag ggactggtga tgggaacccg cagcggtgac atcgatccca gcatcgtcag ctatctgtgc cacacggccg ggatgggggt ggacgacgtc gagtcgatgc tcaaccaccg ctcgggggtg gtggggctgt ccggcgtgcg ggacttccgc cggctgcgcg aactcatcga atccggtgac ggcgcagcgc aattggccta cagcgtgttc acgcaccggc tgcgcaagta catcggagcc tacctggcgg tgctgggcca caccgacgtc atcagcttca ccgccggcat cggtgagaac gacgccgcgg tgcgccgcga cgcggtgtcc ggcatggagg agctgggcat cgtgctcgac gagcgccgca acctcgccgg gggcaaaggg gcacgccaga tctcggccga cgactcgccg atcacggtgc tggtggtccc caccaacgaa gagctggcca tcgcccgtga ctgcgtgcgg gtgctaggcg gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa