MysmA.00289.b
Deoxyribose-phosphate aldolase
| CENTER ID: | MysmA.00289.b |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00289.b.A1.GE28599 | Full length( MysmA.00289.b ) | 1 | 227 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00289.b.A1.PS00550 | Full length( MysmA.00289.b ) | 1 | 227 |
Structures
3NDO
DEPOSITED: 6/7/2010
DETERMINATION: XRay
CLONE: MysmA.00289.b.A1.GE28599
PROTEIN: MysmA.00289.b.A1.PS00550
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127821 |
| PATRIC ID: | fig|246196.19.peg.912 |
| RefSeq: | YP_885325.1 |
| UniProt: | A0QQY7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDHTRAQVA ALVDHTLLKP EATPSDVTAL VDEAADLGVF AVCVSPPLVS VAAGVAPSGL AIAAVAGFPS GKHVPGIKAT EAELAVAAGA TEIDMVIDVG AALAGDLDAV SADITAVRKA VRAATLKVIV ESAALLEFSG EPLLADVCRV ARDAGADFVK TSTGFHPSGG ASVQAVEIMA RTVGERLGVK ASGGIRTAEQ AAAMLDAGAT RLGLSGSRAV LDGFGSA
NT Sequence
ATGACCGACC ACACCCGCGC ACAGGTCGCA GCACTCGTCG ACCACACCCT GCTCAAGCCG GAGGCGACGC CGTCCGACGT CACCGCTCTC GTGGACGAGG CGGCCGACCT GGGAGTCTTC GCGGTCTGCG TATCGCCGCC GCTGGTGTCG GTCGCCGCAG GCGTCGCGCC GTCGGGCCTG GCGATCGCCG CGGTGGCCGG GTTCCCGTCC GGCAAGCACG TGCCCGGTAT CAAGGCCACC GAGGCCGAAC TCGCGGTCGC GGCAGGCGCC ACCGAGATCG ACATGGTGAT CGACGTCGGT GCGGCCCTCG CGGGGGATCT CGACGCCGTG TCCGCCGACA TCACCGCGGT CCGCAAGGCC GTGCGCGCGG CGACGCTCAA GGTCATCGTG GAGTCGGCGG CGCTCCTGGA GTTCTCGGGA GAACCGTTGC TGGCCGATGT GTGCCGCGTC GCGCGCGATG CGGGAGCCGA CTTCGTGAAG ACCTCCACGG GTTTCCACCC GTCCGGCGGC GCGTCGGTAC AGGCGGTCGA GATCATGGCG CGCACCGTGG GCGAGCGCCT CGGGGTCAAG GCCAGCGGCG GGATCCGCAC CGCCGAGCAG GCCGCGGCCA TGCTCGACGC GGGAGCCACC CGGCTGGGCC TGTCCGGCTC ACGCGCGGTG CTCGACGGGT TCGGCTCGGC C
Details for MysmA.00289.b.A1.GE28599
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHTRAQV AALVDHTLLK PEATPSDVTA LVDEAADLGV FAVCVSPPLV SVAAGVAPSG LAIAAVAGFP SGKHVPGIKA TEAELAVAAG ATEIDMVIDV GAALAGDLDA VSADITAVRK AVRAATLKVI VESAALLEFS GEPLLADVCR VARDAGADFV KTSTGFHPSG GASVQAVEIM ARTVGERLGV KASGGIRTAE QAAAMLDAGA TRLGLSGSRA VLDGFGSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg accacacccg cgcacaggtc gcagcactcg tcgaccacac cctgctcaag ccggaggcga cgccgtccga cgtcaccgct ctcgtggacg aggcggccga cctgggagtc ttcgcggtct gcgtatcgcc gccgctggtg tcggtcgccg caggcgtcgc gccgtcgggc ctggcgatcg ccgcggtggc cgggttcccg tccggcaagc acgtgcccgg tatcaaggcc accgaggccg aactcgcggt cgcggcaggc gccaccgaga tcgacatggt gatcgacgtc ggtgcggccc tcgcggggga tctcgacgcc gtgtccgccg acatcaccgc ggtccgcaag gccgtgcgcg cggcgacgct caaggtcatc gtggagtcgg cggcgctcct ggagttctcg ggagaaccgt tgctggccga tgtgtgccgc gtcgcgcgcg atgcgggagc cgacttcgtg aagacctcca cgggtttcca cccgtccggc ggcgcgtcgg tacaggcggt cgagatcatg gcgcgcaccg tgggcgagcg cctcggggtc aaggccagcg gcgggatccg caccgccgag caggccgcgg ccatgctcga cgcgggagcc acccggctgg gcctgtccgg ctcacgcgcg gtgctcgacg ggttcggctc ggcctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHTRAQV AALVDHTLLK PEATPSDVTA LVDEAADLGV FAVCVSPPLV SVAAGVAPSG LAIAAVAGFP SGKHVPGIKA TEAELAVAAG ATEIDMVIDV GAALAGDLDA VSADITAVRK AVRAATLKVI VESAALLEFS GEPLLADVCR VARDAGADFV KTSTGFHPSG GASVQAVEIM ARTVGERLGV KASGGIRTAE QAAAMLDAGA TRLGLSGSRA VLDGFGSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaccac acccgcgcac aggtcgcagc actcgtcgac cacaccctgc tcaagccgga ggcgacgccg tccgacgtca ccgctctcgt ggacgaggcg gccgacctgg gagtcttcgc ggtctgcgta tcgccgccgc tggtgtcggt cgccgcaggc gtcgcgccgt cgggcctggc gatcgccgcg gtggccgggt tcccgtccgg caagcacgtg cccggtatca aggccaccga ggccgaactc gcggtcgcgg caggcgccac cgagatcgac atggtgatcg acgtcggtgc ggccctcgcg ggggatctcg acgccgtgtc cgccgacatc accgcggtcc gcaaggccgt gcgcgcggcg acgctcaagg tcatcgtgga gtcggcggcg ctcctggagt tctcgggaga accgttgctg gccgatgtgt gccgcgtcgc gcgcgatgcg ggagccgact tcgtgaagac ctccacgggt ttccacccgt ccggcggcgc gtcggtacag gcggtcgaga tcatggcgcg caccgtgggc gagcgcctcg gggtcaaggc cagcggcggg atccgcaccg ccgagcaggc cgcggccatg ctcgacgcgg gagccacccg gctgggcctg tccggctcac gcgcggtgct cgacgggttc ggctcggcca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.00289.b.A1.PS00550
| PURIFICATION DATe: | 4/2/2010 |
| CONCENTRATION: | 4.57mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHTRAQV AALVDHTLLK PEATPSDVTA LVDEAADLGV FAVCVSPPLV SVAAGVAPSG LAIAAVAGFP SGKHVPGIKA TEAELAVAAG ATEIDMVIDV GAALAGDLDA VSADITAVRK AVRAATLKVI VESAALLEFS GEPLLADVCR VARDAGADFV KTSTGFHPSG GASVQAVEIM ARTVGERLGV KASGGIRTAE QAAAMLDAGA TRLGLSGSRA VLDGFGSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg accacacccg cgcacaggtc gcagcactcg tcgaccacac cctgctcaag ccggaggcga cgccgtccga cgtcaccgct ctcgtggacg aggcggccga cctgggagtc ttcgcggtct gcgtatcgcc gccgctggtg tcggtcgccg caggcgtcgc gccgtcgggc ctggcgatcg ccgcggtggc cgggttcccg tccggcaagc acgtgcccgg tatcaaggcc accgaggccg aactcgcggt cgcggcaggc gccaccgaga tcgacatggt gatcgacgtc ggtgcggccc tcgcggggga tctcgacgcc gtgtccgccg acatcaccgc ggtccgcaag gccgtgcgcg cggcgacgct caaggtcatc gtggagtcgg cggcgctcct ggagttctcg ggagaaccgt tgctggccga tgtgtgccgc gtcgcgcgcg atgcgggagc cgacttcgtg aagacctcca cgggtttcca cccgtccggc ggcgcgtcgg tacaggcggt cgagatcatg gcgcgcaccg tgggcgagcg cctcggggtc aaggccagcg gcgggatccg caccgccgag caggccgcgg ccatgctcga cgcgggagcc acccggctgg gcctgtccgg ctcacgcgcg gtgctcgacg ggttcggctc ggcctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHTRAQV AALVDHTLLK PEATPSDVTA LVDEAADLGV FAVCVSPPLV SVAAGVAPSG LAIAAVAGFP SGKHVPGIKA TEAELAVAAG ATEIDMVIDV GAALAGDLDA VSADITAVRK AVRAATLKVI VESAALLEFS GEPLLADVCR VARDAGADFV KTSTGFHPSG GASVQAVEIM ARTVGERLGV KASGGIRTAE QAAAMLDAGA TRLGLSGSRA VLDGFGSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGACCAC ACCCGCGCAC AGGTCGCAGC ACTCGTCGAC CACACCCTGC TCAAGCCGGA GGCGACGCCG TCCGACGTCA CCGCTCTCGT GGACGAGGCG GCCGACCTGG GAGTCTTCGC GGTCTGCGTA TCGCCGCCGC TGGTGTCGGT CGCCGCAGGC GTCGCGCCGT CGGGCCTGGC GATCGCCGCG GTGGCCGGGT TCCCGTCCGG CAAGCACGTG CCCGGTATCA AGGCCACCGA GGCCGAACTC GCGGTCGCGG CAGGCGCCAC CGAGATCGAC ATGGTGATCG ACGTCGGTGC GGCCCTCGCG GGGGATCTCG ACGCCGTGTC CGCCGACATC ACCGCGGTCC GCAAGGCCGT GCGCGCGGCG ACGCTCAAGG TCATCGTGGA GTCGGCGGCG CTCCTGGAGT TCTCGGGAGA ACCGTTGCTG GCCGATGTGT GCCGCGTCGC GCGCGATGCG GGAGCCGACT TCGTGAAGAC CTCCACGGGT TTCCACCCGT CCGGCGGCGC GTCGGTACAG GCGGTCGAGA TCATGGCGCG CACCGTGGGC GAGCGCCTCG GGGTCAAGGC CAGCGGCGGG ATCCGCACCG CCGAGCAGGC CGCGGCCATG CTCGACGCGG GAGCCACCCG GCTGGGCCTG TCCGGCTCAC GCGCGGTGCT CGACGGGTTC GGCTCGGCCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A