MyabA.00289.a

Deoxyribose-phosphate aldolase (DeoC)

CENTER ID: MyabA.00289.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00289.a.A1.GE30279 Full length( MyabA.00289.a ) 1 218
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4153
RefSeq: YP_001704805.1
UniProt: B1MHS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAALIDHTLL KPEATPEDVR ALVAEAVDLG VLAVCVSPSM LPVLLPEGVE RLLVAGVVGF PSGKHLSSVK AHEAALAVAA GAHEIDMVID VGAAVGGDFG AVQGDIEAVR RAVPAATLKV IIESAALLER SGPEAVRAAC RASEAAGADF VKTSTGFHPA GGATVEAVRI MAETVGDRLQ VKASGGIRTA RDAAAMLEAG ATRLGLSGSR IVLDGFAD
NT Sequence
GTGGCCGCAC TCATCGACCA CACCCTGCTC AAGCCCGAGG CCACGCCCGA GGACGTGCGG GCGCTCGTTG CGGAGGCGGT AGATCTGGGC GTGCTCGCGG TGTGCGTGTC GCCGTCGATG CTGCCCGTGC TCCTACCCGA AGGCGTCGAG AGACTGCTCG TCGCTGGGGT GGTGGGATTC CCGTCCGGCA AACATCTTTC GTCTGTCAAG GCGCATGAGG CCGCGCTGGC GGTCGCCGCC GGCGCACACG AGATCGACAT GGTGATCGAC GTGGGTGCCG CGGTGGGCGG CGATTTCGGG GCGGTGCAGG GCGATATCGA GGCGGTCCGG CGGGCGGTCC CGGCGGCGAC CCTCAAGGTC ATCATCGAGT CTGCCGCGCT GCTGGAGCGT TCGGGCCCCG AGGCCGTCCG TGCGGCCTGC CGGGCCAGCG AGGCTGCCGG TGCGGATTTC GTGAAGACCT CCACCGGATT TCACCCGGCT GGGGGAGCGA CGGTGGAGGC CGTCCGAATC ATGGCCGAGA CCGTCGGCGA CCGGCTTCAG GTGAAGGCGA GCGGCGGAAT CCGGACCGCA CGGGACGCTG CCGCCATGCT CGAGGCCGGC GCCACCCGGT TAGGCCTGTC GGGTAGCAGG ATCGTTCTGG ACGGCTTCGC CGAC
Details for MyabA.00289.a.A1.GE30279
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 77
PERCENT COVERAGE: 110
Validated AA Sequence
MGTXXAXTXG XGSMVAALID HTLLKPEATP XDVRALXAEA VDLGVLAXCX SPSMLPVLLP XGVXRLLVAG VVGFPSGKHL SSVKXHEXAL AXAAGXHXIX MVIDVGAAVG GDFGAVQGDX EXXRRAVPAA TLKVIIXSAA LLERSGPEAV XAACRXSEAA GAXFXKTSTG FHPAGGATVE AVXIMXETVX DRXQVKXXXG XXTXXXAAXX LXXXXTRLGL XGXXSXWTXX PTNXXXXXXL XSXXXXXLLX XXXXAEXAAX XXXXX
Validated NT Sequence
ttttgtttac tttangaagg agnnatacnn tggctcatca ccntcaccat catatgggta cnnnnnnagc tcanacccnn ggtnctggnt cgatggtggc cgcactcatc gaccacaccc tgctcaagcc cgaggccacg cccnangacg tgcgggcgct cntngcggag gcggtagatc tgggcgtgct cgcggngtgc nngtcgccgt cnatgctgcc cgtgctccta cccnaaggcg tcnagagact gctcgtcgct ggggtggtgg gattcccgtc cggcaaacat ctttcgtctg tcaaggnnca tgaggncgcg ctggcggncg ccgccggnnn ncacganatc nacatggtga tcgacgtggg tgccgcggtg ggcggcgatt tcggggcggt gcagggggat ntngaggnng nccggcgggc ggtcccngcn gcnacnctna aggtcatcat cnantctgcc gcgctgctgg agcgttcggg ccccgaggcc gtccntgcng cctgccgggn cagcgaggct gccggngcnn atttcntgaa gacctccacc ggatttcacc cngctggggg agcnacngtg gaggccgtcc naatcatggn cgagaccgtc ngcgaccggn ttcaggtgaa ggngagngnc ggantccnga ccnnnnngna cgctgccgnn ntgctcgnnn nnngnnncac ncggttaggc ctgncgggtn nnngatcgnn ctggacggnt ncnccnacta atanacnnnn nnnnnnannn ctggnaagct nntnnngnnn ncngctgcta ncnannnnan nggnagctga gnnggctgcn nccnncnnnn annnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAALIDHT LLKPEATPED VRALVAEAVD LGVLAVCVSP SMLPVLLPEG VERLLVAGVV GFPSGKHLSS VKAHEAALAV AAGAHEIDMV IDVGAAVGGD FGAVQGDIEA VRRAVPAATL KVIIESAALL ERSGPEAVRA ACRASEAAGA DFVKTSTGFH PAGGATVEAV RIMAETVGDR LQVKASGGIR TARDAAAMLE AGATRLGLSG SRIVLDGFAD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgca ctcatcgacc acaccctgct caagcccgag gccacgcccg aggacgtgcg ggcgctcgtt gcggaggcgg tagatctggg cgtgctcgcg gtgtgcgtgt cgccgtcgat gctgcccgtg ctcctacccg aaggcgtcga gagactgctc gtcgctgggg tggtgggatt cccgtccggc aaacatcttt cgtctgtcaa ggcgcatgag gccgcgctgg cggtcgccgc cggcgcacac gagatcgaca tggtgatcga cgtgggtgcc gcggtgggcg gcgatttcgg ggcggtgcag ggcgatatcg aggcggtccg gcgggcggtc ccggcggcga ccctcaaggt catcatcgag tctgccgcgc tgctggagcg ttcgggcccc gaggccgtcc gtgcggcctg ccgggccagc gaggctgccg gtgcggattt cgtgaagacc tccaccggat ttcacccggc tgggggagcg acggtggagg ccgtccgaat catggccgag accgtcggcg accggcttca ggtgaaggcg agcggcggaa tccggaccgc acgggacgct gccgccatgc tcgaggccgg cgccacccgg ttaggcctgt cgggtagcag gatcgttctg gacggcttcg ccgacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa