SSGCID: Target Page

MymaA.00289.a

Deoxyribose-phosphate aldolase

CENTER ID:	MymaA.00289.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00289.a.A1.GE29984	Full length( MymaA.00289.a )		1	226

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.857
RefSeq:	YP_001849118.1
UniProt:	B2HQU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPGQPTRDQV AALVDHTLLK PEATAADVVA LVAEAADLGV YAVCVSPSMV PAAVSAGGVR VATVAGFPSG KHASAIKAHE AALAVACGAV EVDMVIDVGA ALAGHLDAVR SDIEAVRCAT SGAVLKVIVE SAALLGLADE STLIGVCRVA EDAGADFVKT STGFHPAGGA STRAVEVMAS AVGGRLGVKA SGGIRTATDA VAMLSAGATR LGLSGTRAVL EGLGQN

NT Sequence

ATGCCGGGTC AACCAACCCG AGACCAGGTG GCGGCGTTGG TGGATCACAC CCTGCTCAAG CCCGAGGCTA CTGCCGCCGA TGTGGTGGCC CTGGTGGCAG AAGCCGCCGA CCTCGGCGTT TACGCGGTCT GCGTTTCGCC GTCGATGGTG CCCGCCGCGG TCTCGGCCGG CGGCGTGCGG GTAGCCACGG TGGCGGGTTT CCCGTCGGGC AAGCATGCCT CGGCGATCAA GGCGCATGAG GCGGCGCTTG CCGTTGCCTG CGGTGCCGTT GAGGTCGACA TGGTCATCGA TGTCGGTGCC GCGCTGGCCG GGCACCTCGA TGCGGTGCGG TCCGACATCG AGGCGGTGCG TTGCGCGACC TCCGGGGCGG TGCTGAAGGT GATCGTTGAG TCGGCGGCGC TATTGGGTCT GGCCGACGAG TCCACGCTGA TCGGCGTGTG CCGGGTCGCC GAGGACGCCG GAGCGGACTT CGTCAAGACG TCTACCGGCT TTCATCCCGC AGGCGGCGCC TCGACGCGCG CCGTCGAAGT GATGGCCAGC GCCGTCGGCG GACGACTCGG GGTCAAGGCC AGCGGCGGCA TCCGGACCGC CACCGACGCG GTCGCGATGC TCAGTGCCGG TGCAACCAGG CTGGGCCTGT CCGGCACCCG AGCGGTCCTC GAGGGGCTCG GCCAGAAC

Details for MymaA.00289.a.A1.GE29984

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/18/2010
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPGQPTRDQ VAALVDHTLL KPEATAADVV ALVAEAADLG VYAVCVSPSM VPAAVSAGGV RVATVAGFPS GKHASAIKAH EAALAVACGA VEVDMVIDVG AALAGHLDAV RSDIEAVRCA TSGAVLKVIV ESAALLGLAD ESTLIGVCRV AEDAGADFVK TSTGFHPAGG ASTRAVEVMA SAVGGRLGVK ASGGIRTATD AVAMLSAGAT RLGLSGTRAV LEGLGQ

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgg gtcaaccaac ccgagaccag gtggcggcgt tggtggatca caccctgctc aagcccgagg ctactgccgc cgatgtggtg gccctggtgg cagaagccgc cgacctcggc gtttacgcgg tctgcgtttc gccgtcgatg gtgcccgccg cggtctcggc cggcggcgtg cgggtagcca cggtggcggg tttcccgtcg ggcaagcatg cctcggcgat caaggcgcat gaggcggcgc ttgccgttgc ctgcggtgcc gttgaggtcg acatggtcat cgatgtcggt gccgcgctgg ccgggcacct cgatgcggtg cggtccgaca tcgaggcggt gcgttgcgcg acctccgggg cggtgctgaa ggtgatcgtt gagtcggcgg cgctattggg tctggccgac gagtccacgc tgatcggcgt gtgccgggtc gccgaggacg ccggagcgga cttcgtcaag acgtctaccg gctttcatcc cgcaggcggc gcctcgacgc gcgccgtcga agtgatggcc agcgccgtcg gcggacgact cggggtcaag gccagcggcg gcatccggac cgccaccgac gcggtcgcga tgctcagtgc cggtgcaacc aggctgggcc tgtccggcac ccgagcggtc ctcgaggggc tcggccagta ataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgggtcaa ccaacccgag accaggtggc ggcgttggtg gatcacaccc tgctcaagcc cgaggctact gccgccgatg tggtggccct ggtggcagaa gccgccgacc tcggcgttta cgcggtctgc gtttcgccgt cgatggtgcc cgccgcggtc tcggccggcg gcgtgcgggt agccacggtg gcgggtttcc cgtcgggcaa gcatgcctcg gcgatcaagg cgcatgaggc ggcgcttgcc gttgcctgcg gtgccgttga ggtcgacatg gtcatcgatg tcggtgccgc gctggccggg cacctcgatg cggtgcggtc cgacatcgag gcggtgcgtt gcgcgacctc cggggcggtg ctgaaggtga tcgttgagtc ggcggcgcta ttgggtctgg ccgacgagtc cacgctgatc ggcgtgtgcc gggtcgccga ggacgccgga gcggacttcg tcaagacgtc taccggcttt catcccgcag gcggcgcctc gacgcgcgcc gtcgaagtga tggccagcgc cgtcggcgga cgactcgggg tcaaggccag cggcggcatc cggaccgcca ccgacgcggt cgcgatgctc agtgccggtg caaccaggct gggcctgtcc ggcacccgag cggtcctcga ggggctcggc cagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa