MyavA.00289.a
Deoxyribose-phosphate aldolase
| CENTER ID: | MyavA.00289.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00289.a.A1.GE28504 | Full length( MyavA.00289.a ) | 1 | 223 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00289.a.A1.PS00578 | Full length( MyavA.00289.a ) | 1 | 223 |
Structures
3NG3
DEPOSITED: 6/10/2010
DETERMINATION: XRay
CLONE: MyavA.00289.a.A1.GE28504
PROTEIN: MyavA.00289.a.A1.PS00578
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127821 |
| PATRIC ID: | fig|243243.7.peg.4590 |
| RefSeq: | YP_883800.1 |
| UniProt: | A0QLL2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPTRAQLAA FVDHTLLKPE ATAADVAALV TEAAELGVYA VCVSPPMVPA AVQAGAGVRV ASVAGFPSGK HVSAVKAHEA ALAVASGAAE IDMVIDVGAA LAGDLDGVRA DIAAVRGAVG GAVLKVIVES SALLALADEH TLVRVCRAAE DAGADFVKTS TGFHPSGGAS VRAVALMAEA VGGRLGVKAS GGIRTAADAL AMLDAGATRL GLSGTRAVLD GLG
NT Sequence
ATGACCCCCA CCCGCGCCCA GCTGGCGGCA TTCGTCGACC ACACCCTGCT CAAGCCCGAA GCCACCGCCG CCGACGTCGC CGCCCTGGTC ACCGAGGCGG CCGAGCTGGG CGTCTACGCG GTGTGCGTCT CGCCGCCGAT GGTTCCCGCC GCCGTGCAGG CCGGTGCCGG CGTGCGGGTG GCCAGCGTGG CGGGATTCCC GTCGGGCAAG CATGTGTCGG CCGTCAAGGC CCACGAGGCC GCGCTGGCGG TGGCCTCCGG TGCCGCCGAG ATCGACATGG TCATCGACGT CGGCGCGGCG CTGGCCGGTG ACCTCGACGG GGTGCGCGCC GACATCGCGG CGGTGCGCGG CGCGGTCGGC GGCGCCGTGC TCAAGGTGAT CGTGGAGTCG TCAGCGCTGC TGGCGCTGGC CGACGAGCAC ACCCTGGTCC GGGTGTGCCG CGCCGCCGAG GACGCCGGCG CCGACTTCGT CAAGACGTCG ACCGGATTCC ACCCGTCCGG CGGCGCATCC GTGCGGGCGG TCGCCCTGAT GGCCGAGGCC GTCGGGGGCC GGCTGGGGGT GAAGGCCAGC GGCGGCATCC GCACCGCCGC CGATGCGCTG GCGATGCTGG ATGCCGGCGC CACCCGGCTG GGATTGTCGG GCACCCGGGC CGTGCTCGAC GGGCTGGGC
Details for MyavA.00289.a.A1.GE28504
| HARVESTED ON: | 2/9/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPTRAQLA AFVDHTLLKP EATAADVAAL VTEAAELGVY AVCVSPPMVP AAVQAGAGVR VASVAGFPSG KHVSAVKAHE AALAVASGAA EIDMVIDVGA ALAGDLDGVR ADIAAVRGAV GGAVLKVIVE SSALLALADE HTLVRVCRAA EDAGADFVKT STGFHPSGGA SVRAVALMAE AVGGRLGVKA SGGIRTAADA LAMLDAGATR LGLSGTRAVL DGLG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccc ccacccgcgc ccagctggcg gcattcgtcg accacaccct gctcaagccc gaagccaccg ccgccgacgt cgccgccctg gtcaccgagg cggccgagct gggcgtctac gcggtgtgcg tctcgccgcc gatggttccc gccgccgtgc aggccggtgc cggcgtgcgg gtggccagcg tggcgggatt cccgtcgggc aagcatgtgt cggccgtcaa ggcccacgag gccgcgctgg cggtggcctc cggtgccgcc gagatcgaca tggtcatcga cgtcggcgcg gcgctggccg gtgacctcga cggggtgcgc gccgacatcg cggcggtgcg cggcgcggtc ggcggcgccg tgctcaaggt gatcgtggag tcgtcagcgc tgctggcgct ggccgacgag cacaccctgg tccgggtgtg ccgcgccgcc gaggacgccg gcgccgactt cgtcaagacg tcgaccggat tccacccgtc cggcggcgca tccgtgcggg cggtcgccct gatggccgag gccgtcgggg gccggctggg ggtgaaggcc agcggcggca tccgcaccgc cgccgatgcg ctggcgatgc tggatgccgg cgccacccgg ctgggattgt cgggcacccg ggccgtgctc gacgggctgg gctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPTRAQLA AFVDHTLLKP EATAADVAAL VTEAAELGVY AVCVSPPMVP AAVQAGAGVR VASVAGFPSG KHVSAVKAHE AALAVASGAA EIDMVIDVGA ALAGDLDGVR ADIAAVRGAV GGAVLKVIVE SSALLALADE HTLVRVCRAA EDAGADFVKT STGFHPSGGA SVRAVALMAE AVGGRLGVKA SGGIRTAADA LAMLDAGATR LGLSGTRAVL DGLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccccacc cgcgcccagc tggcggcatt cgtcgaccac accctgctca agcccgaagc caccgccgcc gacgtcgccg ccctggtcac cgaggcggcc gagctgggcg tctacgcggt gtgcgtctcg ccgccgatgg ttcccgccgc cgtgcaggcc ggtgccggcg tgcgggtggc cagcgtggcg ggattcccgt cgggcaagca tgtgtcggcc gtcaaggccc acgaggccgc gctggcggtg gcctccggtg ccgccgagat cgacatggtc atcgacgtcg gcgcggcgct ggccggtgac ctcgacgggg tgcgcgccga catcgcggcg gtgcgcggcg cggtcggcgg cgccgtgctc aaggtgatcg tggagtcgtc agcgctgctg gcgctggccg acgagcacac cctggtccgg gtgtgccgcg ccgccgagga cgccggcgcc gacttcgtca agacgtcgac cggattccac ccgtccggcg gcgcatccgt gcgggcggtc gccctgatgg ccgaggccgt cgggggccgg ctgggggtga aggccagcgg cggcatccgc accgccgccg atgcgctggc gatgctggat gccggcgcca cccggctggg attgtcgggc acccgggccg tgctcgacgg gctgggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyavA.00289.a.A1.PS00578
| PURIFICATION DATe: | 4/29/2010 |
| CONCENTRATION: | 60.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPTRAQLA AFVDHTLLKP EATAADVAAL VTEAAELGVY AVCVSPPMVP AAVQAGAGVR VASVAGFPSG KHVSAVKAHE AALAVASGAA EIDMVIDVGA ALAGDLDGVR ADIAAVRGAV GGAVLKVIVE SSALLALADE HTLVRVCRAA EDAGADFVKT STGFHPSGGA SVRAVALMAE AVGGRLGVKA SGGIRTAADA LAMLDAGATR LGLSGTRAVL DGLG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccc ccacccgcgc ccagctggcg gcattcgtcg accacaccct gctcaagccc gaagccaccg ccgccgacgt cgccgccctg gtcaccgagg cggccgagct gggcgtctac gcggtgtgcg tctcgccgcc gatggttccc gccgccgtgc aggccggtgc cggcgtgcgg gtggccagcg tggcgggatt cccgtcgggc aagcatgtgt cggccgtcaa ggcccacgag gccgcgctgg cggtggcctc cggtgccgcc gagatcgaca tggtcatcga cgtcggcgcg gcgctggccg gtgacctcga cggggtgcgc gccgacatcg cggcggtgcg cggcgcggtc ggcggcgccg tgctcaaggt gatcgtggag tcgtcagcgc tgctggcgct ggccgacgag cacaccctgg tccgggtgtg ccgcgccgcc gaggacgccg gcgccgactt cgtcaagacg tcgaccggat tccacccgtc cggcggcgca tccgtgcggg cggtcgccct gatggccgag gccgtcgggg gccggctggg ggtgaaggcc agcggcggca tccgcaccgc cgccgatgcg ctggcgatgc tggatgccgg cgccacccgg ctgggattgt cgggcacccg ggccgtgctc gacgggctgg gctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPTRAQLA AFVDHTLLKP EATAADVAAL VTEAAELGVY AVCVSPPMVP AAVQAGAGVR VASVAGFPSG KHVSAVKAHE AALAVASGAA EIDMVIDVGA ALAGDLDGVR ADIAAVRGAV GGAVLKVIVE SSALLALADE HTLVRVCRAA EDAGADFVKT STGFHPSGGA SVRAVALMAE AVGGRLGVKA SGGIRTAADA LAMLDAGATR LGLSGTRAVL DGLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCCCACC CGCGCCCAGC TGGCGGCATT CGTCGACCAC ACCCTGCTCA AGCCCGAAGC CACCGCCGCC GACGTCGCCG CCCTGGTCAC CGAGGCGGCC GAGCTGGGCG TCTACGCGGT GTGCGTCTCG CCGCCGATGG TTCCCGCCGC CGTGCAGGCC GGTGCCGGCG TGCGGGTGGC CAGCGTGGCG GGATTCCCGT CGGGCAAGCA TGTGTCGGCC GTCAAGGCCC ACGAGGCCGC GCTGGCGGTG GCCTCCGGTG CCGCCGAGAT CGACATGGTC ATCGACGTCG GCGCGGCGCT GGCCGGTGAC CTCGACGGGG TGCGCGCCGA CATCGCGGCG GTGCGCGGCG CGGTCGGCGG CGCCGTGCTC AAGGTGATCG TGGAGTCGTC AGCGCTGCTG GCGCTGGCCG ACGAGCACAC CCTGGTCCGG GTGTGCCGCG CCGCCGAGGA CGCCGGCGCC GACTTCGTCA AGACGTCGAC CGGATTCCAC CCGTCCGGCG GCGCATCCGT GCGGGCGGTC GCCCTGATGG CCGAGGCCGT CGGGGGCCGG CTGGGGGTGA AGGCCAGCGG CGGCATCCGC ACCGCCGCCG ATGCGCTGGC GATGCTGGAT GCCGGCGCCA CCCGGCTGGG ATTGTCGGGC ACCCGGGCCG TGCTCGACGG GCTGGGCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA