MyabA.01507.a
Probable fumarate hydratase Fum
| CENTER ID: | MyabA.01507.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01507.a.A1.GE30515 | Full length( MyabA.01507.a ) | 1 | 466 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01507.a.A1.PW30392 | Full length( MyabA.01507.a ) | 1 | 466 |
Structures
3RRP
DEPOSITED: 4/29/2011
DETERMINATION: XRay
CLONE: MyabA.01507.a.A1.GE30515
PROTEIN: MyabA.01507.a.A1.PW30392
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.1287 |
| RefSeq: | YP_001701992.1 |
| UniProt: | B1MKP6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEQQYRIEH DTMGEVRVPA EALWRAQTQR AVENFPISGR GLERTQIRAL GLLKGACAQV NKDLGLLAAE KADAIIAAAQ EIADGKHDDQ FPIDVFQTGS GTSSNMNANE VIASIAAQAT PPVVVHPNDD VNMSQSSNDT FPTATHLAAT EAAVRDLIPA LEYLQQALAT KAKAWKTVVK SGRTHLMDAV PVTLGQEFGG YARQIEAGIE RVKATLPRLG ELPIGGTAVG TGLNAPDGFG AKVVEVLKQS TGLSELKTAS DSFEAQAARD GLVEGSGALK TIAASLTKIA NDIRWMGSGP LTGLGEIQLP DLQPGSSIMP GKVNPVLPEA VTQVAAQVIG NDAAITVGGL SGAFELNVYI PVMARNLLES FTLLANVSRL FVDKCVDGLV ANEDHLRTLA ESSPSIVTPL NSAIGYEEAA AVAKEALKER KTIRQTVIDR GLIGDKLSIE ELDKRLDVLA MAKVKD
NT Sequence
TTGTCTGAAC AGCAGTACCG CATCGAGCAC GACACCATGG GCGAGGTCCG GGTGCCCGCG GAAGCACTGT GGCGCGCGCA GACCCAGCGC GCGGTCGAGA ACTTTCCCAT CTCGGGACGC GGCCTGGAGC GCACGCAGAT CCGGGCCCTC GGCCTGCTGA AGGGCGCCTG CGCACAGGTC AACAAGGACC TGGGGCTGCT GGCTGCGGAG AAGGCCGATG CGATTATCGC TGCGGCACAG GAGATCGCCG ACGGAAAGCA CGACGACCAG TTCCCCATCG ATGTCTTCCA GACCGGCTCG GGCACCAGCT CGAATATGAA CGCCAACGAG GTGATCGCCA GTATTGCGGC ACAGGCGACG CCACCGGTTG TCGTGCACCC GAATGACGAC GTGAACATGT CGCAGTCCTC CAATGACACC TTCCCGACGG CGACCCACCT GGCCGCGACC GAAGCTGCTG TGCGCGACCT GATTCCGGCA CTGGAGTACC TCCAGCAGGC ACTGGCCACC AAGGCCAAGG CCTGGAAGAC CGTCGTGAAG TCCGGCCGCA CGCACCTAAT GGACGCGGTT CCGGTGACGC TGGGCCAGGA GTTCGGCGGC TACGCCCGTC AGATCGAGGC CGGCATAGAA CGGGTCAAGG CGACGCTGCC CCGCCTCGGC GAGCTGCCCA TCGGCGGTAC CGCCGTGGGC ACCGGCCTGA ACGCCCCCGA CGGCTTTGGT GCGAAAGTTG TTGAGGTACT GAAGCAGTCG ACGGGCCTGT CGGAGCTCAA GACCGCTTCT GACTCGTTCG AGGCACAGGC CGCCCGTGAC GGGCTGGTTG AGGGCTCGGG CGCGCTGAAG ACCATCGCGG CATCGCTGAC CAAGATCGCC AACGACATTC GCTGGATGGG CTCGGGCCCG CTCACCGGCC TGGGCGAGAT CCAGCTTCCC GATCTGCAGC CCGGCAGCTC GATCATGCCC GGCAAGGTCA ACCCGGTGCT GCCCGAGGCC GTCACACAGG TCGCGGCGCA GGTCATCGGT AACGATGCCG CCATCACGGT GGGTGGCTTG TCCGGCGCCT TCGAGCTCAA CGTGTACATC CCGGTCATGG CCCGCAACCT GCTGGAGTCG TTCACGCTGC TGGCCAACGT ATCTCGCCTG TTCGTCGACA AGTGTGTCGA TGGCCTGGTG GCCAACGAGG ATCACCTGCG GACGCTGGCG GAGTCCTCGC CGTCAATCGT TACCCCGTTG AACTCGGCCA TCGGCTATGA AGAAGCGGCG GCCGTCGCCA AGGAAGCGCT CAAGGAGCGC AAGACGATTC GGCAGACGGT CATCGATCGC GGCCTCATCG GCGACAAGCT GAGCATCGAG GAACTGGACA AGCGCCTCGA CGTGCTCGCC ATGGCAAAGG TGAAGGAC
Details for MyabA.01507.a.A1.GE30515
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 9/28/2010 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 84 |
| PERCENT COVERAGE: | 50 |
Validated AA Sequence
MLSEQQYRIE HDTMGEVRVP AEALWRAQTQ RAVENFPISG RGLERTQIRA LGLLKGACAQ VNKDLGLLAA EKADAIIAAA QEIADGKHDD QFPIDVFQTG SGTSSNMNAN EVIASIAAQA TPPVVVHPND DVNMSQSSND TFPTATHLAA TEAAVRDLIP ALEYLQQALA TKAKAWKTVV KSGRTHLMDA VPGDAGPGVR RLRPSDRGRH RTGQGDAAPP RRAAHRRYRR GHRPERPRRL WCESC
Validated NT Sequence
tcacctttgc catggcgagc acgtcgaggc gcttgtccag ttcctcgatg ctcagcttgt cgccgatgag gccgcgatcg atgaccgtct gccgaatcgt cttgcgctcc ttgagcgctt ccttggcgac ggccgccgct tcttcatagc cgatggccga gttcaacggg gtaacgattg acggcgagga ctccgccagc gtccgcaggt gatcctcgtt ggccaccagg ccatcgacac acttgtcgac gaacaggcga gatacgttgg ccagcagcgt gaacgactcc agcaggttgc gggccatgac cgggatgtac acgttgagct cgaaggcgcc ggacaagcca cccaccgtga tggcggcatc gttaccgatg acctgcgccg cgacctgtgt gacggcctcg ggcagcaccg ggttgacctt gccgggcatg atcgagctgc cgggctgcag atcgggaagc tggatctcgc ccaggccggt gagcgggccc gagcccatcc agcgaatgtc gttggcgatc ttggtcagcg atgccgcgat ggtcttcagc gcgcccgagc cctcaaccag cccgtcacgg gcggcctgtg cctcgaacga gtcagaagcg gtcttgagct ccgacaggcc cgtcgactgc ttcagtacct caacaacttt cgcaccaaag ccgtcggggg cgttcaggcc ggtgcccacg gcggtaccgc cgatgggcag ctcgccgagg cggggcagcg tcgccttgac ccgttctatg ccggcctcga tctgacgggc gtagccgccg aactcctggc ccagcgtcac cgggaaccgc gtccattagg tgcgtgcggc cggacttcac gacggtcttc caggccttgg ccttggtggc cagtgcctgc tggaggtact ccagtgccgg aatcaggtcg cgcacagcag cttcggtcgc ggccaggtgg gtcgccgtcg ggaaggtgtc attggaggac tgcgacatgt tcacgtcgtc attcgggtgc acgacaaccg gtggcgtcgc ctgtgccgca atactggcga tcacctcgtt ggcgttcata ttcgagctgg tgcccgagcc ggtctggaag acatcgatgg ggaactggtc gtcgtgcttt ccgtcggcga tctcctgtgc cgcagcgata atcgcatcgg ccttctccgc agccagcagc cccaggtcct tgttgacctg tgcgcaggcg cccttcagca ggccgagggc ccggatctgc gtgcgctcca ggccgcgtcc cgagatggga aagttctcga ccgcgcgctg ggtctgcgcg cgccacagtg cttccgcggg cacccggacc tcgcccatgg tgtcgtgctc gatgcggtac tgctgttcag acaacatcga accaggaccc tgggtctgag cttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSEQQYRI EHDTMGEVRV PAEALWRAQT QRAVENFPIS GRGLERTQIR ALGLLKGACA QVNKDLGLLA AEKADAIIAA AQEIADGKHD DQFPIDVFQT GSGTSSNMNA NEVIASIAAQ ATPPVVVHPN DDVNMSQSSN DTFPTATHLA ATEAAVRDLI PALEYLQQAL ATKAKAWKTV VKSGRTHLMD AVPVTLGQEF GGYARQIEAG IERVKATLPR LGELPIGGTA VGTGLNAPDG FGAKVVEVLK QSTGLSELKT ASDSFEAQAA RDGLVEGSGA LKTIAASLTK IANDIRWMGS GPLTGLGEIQ LPDLQPGSSI MPGKVNPVLP EAVTQVAAQV IGNDAAITVG GLSGAFELNV YIPVMARNLL ESFTLLANVS RLFVDKCVDG LVANEDHLRT LAESSPSIVT PLNSAIGYEE AAAVAKEALK ERKTIRQTVI DRGLIGDKLS IEELDKRLDV LAMAKVKD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtctgaa cagcagtacc gcatcgagca cgacaccatg ggcgaggtcc gggtgcccgc ggaagcactg tggcgcgcgc agacccagcg cgcggtcgag aactttccca tctcgggacg cggcctggag cgcacgcaga tccgggccct cggcctgctg aagggcgcct gcgcacaggt caacaaggac ctggggctgc tggctgcgga gaaggccgat gcgattatcg ctgcggcaca ggagatcgcc gacggaaagc acgacgacca gttccccatc gatgtcttcc agaccggctc gggcaccagc tcgaatatga acgccaacga ggtgatcgcc agtattgcgg cacaggcgac gccaccggtt gtcgtgcacc cgaatgacga cgtgaacatg tcgcagtcct ccaatgacac cttcccgacg gcgacccacc tggccgcgac cgaagctgct gtgcgcgacc tgattccggc actggagtac ctccagcagg cactggccac caaggccaag gcctggaaga ccgtcgtgaa gtccggccgc acgcacctaa tggacgcggt tccggtgacg ctgggccagg agttcggcgg ctacgcccgt cagatcgagg ccggcataga acgggtcaag gcgacgctgc cccgcctcgg cgagctgccc atcggcggta ccgccgtggg caccggcctg aacgcccccg acggctttgg tgcgaaagtt gttgaggtac tgaagcagtc gacgggcctg tcggagctca agaccgcttc tgactcgttc gaggcacagg ccgcccgtga cgggctggtt gagggctcgg gcgcgctgaa gaccatcgcg gcatcgctga ccaagatcgc caacgacatt cgctggatgg gctcgggccc gctcaccggc ctgggcgaga tccagcttcc cgatctgcag cccggcagct cgatcatgcc cggcaaggtc aacccggtgc tgcccgaggc cgtcacacag gtcgcggcgc aggtcatcgg taacgatgcc gccatcacgg tgggtggctt gtccggcgcc ttcgagctca acgtgtacat cccggtcatg gcccgcaacc tgctggagtc gttcacgctg ctggccaacg tatctcgcct gttcgtcgac aagtgtgtcg atggcctggt ggccaacgag gatcacctgc ggacgctggc ggagtcctcg ccgtcaatcg ttaccccgtt gaactcggcc atcggctatg aagaagcggc ggccgtcgcc aaggaagcgc tcaaggagcg caagacgatt cggcagacgg tcatcgatcg cggcctcatc ggcgacaagc tgagcatcga ggaactggac aagcgcctcg acgtgctcgc catggcaaag gtgaaggact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.01507.a.A1.PW30392
| PURIFICATION DATe: | 12/13/2010 |
| CONCENTRATION: | 29.54mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 84 |
| PERCENT COVERAGE: | 50 |
Protocol Notes
notes unavailable
Validated AA Sequence
MLSEQQYRIE HDTMGEVRVP AEALWRAQTQ RAVENFPISG RGLERTQIRA LGLLKGACAQ VNKDLGLLAA EKADAIIAAA QEIADGKHDD QFPIDVFQTG SGTSSNMNAN EVIASIAAQA TPPVVVHPND DVNMSQSSND TFPTATHLAA TEAAVRDLIP ALEYLQQALA TKAKAWKTVV KSGRTHLMDA VPGDAGPGVR RLRPSDRGRH RTGQGDAAPP RRAAHRRYRR GHRPERPRRL WCESC
Validated NT Sequence
tcacctttgc catggcgagc acgtcgaggc gcttgtccag ttcctcgatg ctcagcttgt cgccgatgag gccgcgatcg atgaccgtct gccgaatcgt cttgcgctcc ttgagcgctt ccttggcgac ggccgccgct tcttcatagc cgatggccga gttcaacggg gtaacgattg acggcgagga ctccgccagc gtccgcaggt gatcctcgtt ggccaccagg ccatcgacac acttgtcgac gaacaggcga gatacgttgg ccagcagcgt gaacgactcc agcaggttgc gggccatgac cgggatgtac acgttgagct cgaaggcgcc ggacaagcca cccaccgtga tggcggcatc gttaccgatg acctgcgccg cgacctgtgt gacggcctcg ggcagcaccg ggttgacctt gccgggcatg atcgagctgc cgggctgcag atcgggaagc tggatctcgc ccaggccggt gagcgggccc gagcccatcc agcgaatgtc gttggcgatc ttggtcagcg atgccgcgat ggtcttcagc gcgcccgagc cctcaaccag cccgtcacgg gcggcctgtg cctcgaacga gtcagaagcg gtcttgagct ccgacaggcc cgtcgactgc ttcagtacct caacaacttt cgcaccaaag ccgtcggggg cgttcaggcc ggtgcccacg gcggtaccgc cgatgggcag ctcgccgagg cggggcagcg tcgccttgac ccgttctatg ccggcctcga tctgacgggc gtagccgccg aactcctggc ccagcgtcac cgggaaccgc gtccattagg tgcgtgcggc cggacttcac gacggtcttc caggccttgg ccttggtggc cagtgcctgc tggaggtact ccagtgccgg aatcaggtcg cgcacagcag cttcggtcgc ggccaggtgg gtcgccgtcg ggaaggtgtc attggaggac tgcgacatgt tcacgtcgtc attcgggtgc acgacaaccg gtggcgtcgc ctgtgccgca atactggcga tcacctcgtt ggcgttcata ttcgagctgg tgcccgagcc ggtctggaag acatcgatgg ggaactggtc gtcgtgcttt ccgtcggcga tctcctgtgc cgcagcgata atcgcatcgg ccttctccgc agccagcagc cccaggtcct tgttgacctg tgcgcaggcg cccttcagca ggccgagggc ccggatctgc gtgcgctcca ggccgcgtcc cgagatggga aagttctcga ccgcgcgctg ggtctgcgcg cgccacagtg cttccgcggg cacccggacc tcgcccatgg tgtcgtgctc gatgcggtac tgctgttcag acaacatcga accaggaccc tgggtctgag cttccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSEQQYRI EHDTMGEVRV PAEALWRAQT QRAVENFPIS GRGLERTQIR ALGLLKGACA QVNKDLGLLA AEKADAIIAA AQEIADGKHD DQFPIDVFQT GSGTSSNMNA NEVIASIAAQ ATPPVVVHPN DDVNMSQSSN DTFPTATHLA ATEAAVRDLI PALEYLQQAL ATKAKAWKTV VKSGRTHLMD AVPVTLGQEF GGYARQIEAG IERVKATLPR LGELPIGGTA VGTGLNAPDG FGAKVVEVLK QSTGLSELKT ASDSFEAQAA RDGLVEGSGA LKTIAASLTK IANDIRWMGS GPLTGLGEIQ LPDLQPGSSI MPGKVNPVLP EAVTQVAAQV IGNDAAITVG GLSGAFELNV YIPVMARNLL ESFTLLANVS RLFVDKCVDG LVANEDHLRT LAESSPSIVT PLNSAIGYEE AAAVAKEALK ERKTIRQTVI DRGLIGDKLS IEELDKRLDV LAMAKVKD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTCTGAA CAGCAGTACC GCATCGAGCA CGACACCATG GGCGAGGTCC GGGTGCCCGC GGAAGCACTG TGGCGCGCGC AGACCCAGCG CGCGGTCGAG AACTTTCCCA TCTCGGGACG CGGCCTGGAG CGCACGCAGA TCCGGGCCCT CGGCCTGCTG AAGGGCGCCT GCGCACAGGT CAACAAGGAC CTGGGGCTGC TGGCTGCGGA GAAGGCCGAT GCGATTATCG CTGCGGCACA GGAGATCGCC GACGGAAAGC ACGACGACCA GTTCCCCATC GATGTCTTCC AGACCGGCTC GGGCACCAGC TCGAATATGA ACGCCAACGA GGTGATCGCC AGTATTGCGG CACAGGCGAC GCCACCGGTT GTCGTGCACC CGAATGACGA CGTGAACATG TCGCAGTCCT CCAATGACAC CTTCCCGACG GCGACCCACC TGGCCGCGAC CGAAGCTGCT GTGCGCGACC TGATTCCGGC ACTGGAGTAC CTCCAGCAGG CACTGGCCAC CAAGGCCAAG GCCTGGAAGA CCGTCGTGAA GTCCGGCCGC ACGCACCTAA TGGACGCGGT TCCGGTGACG CTGGGCCAGG AGTTCGGCGG CTACGCCCGT CAGATCGAGG CCGGCATAGA ACGGGTCAAG GCGACGCTGC CCCGCCTCGG CGAGCTGCCC ATCGGCGGTA CCGCCGTGGG CACCGGCCTG AACGCCCCCG ACGGCTTTGG TGCGAAAGTT GTTGAGGTAC TGAAGCAGTC GACGGGCCTG TCGGAGCTCA AGACCGCTTC TGACTCGTTC GAGGCACAGG CCGCCCGTGA CGGGCTGGTT GAGGGCTCGG GCGCGCTGAA GACCATCGCG GCATCGCTGA CCAAGATCGC CAACGACATT CGCTGGATGG GCTCGGGCCC GCTCACCGGC CTGGGCGAGA TCCAGCTTCC CGATCTGCAG CCCGGCAGCT CGATCATGCC CGGCAAGGTC AACCCGGTGC TGCCCGAGGC CGTCACACAG GTCGCGGCGC AGGTCATCGG TAACGATGCC GCCATCACGG TGGGTGGCTT GTCCGGCGCC TTCGAGCTCA ACGTGTACAT CCCGGTCATG GCCCGCAACC TGCTGGAGTC GTTCACGCTG CTGGCCAACG TATCTCGCCT GTTCGTCGAC AAGTGTGTCG ATGGCCTGGT GGCCAACGAG GATCACCTGC GGACGCTGGC GGAGTCCTCG CCGTCAATCG TTACCCCGTT GAACTCGGCC ATCGGCTATG AAGAAGCGGC GGCCGTCGCC AAGGAAGCGC TCAAGGAGCG CAAGACGATT CGGCAGACGG TCATCGATCG CGGCCTCATC GGCGACAAGC TGAGCATCGA GGAACTGGAC AAGCGCCTCG ACGTGCTCGC CATGGCAAAG GTGAAGGACT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA