MymaA.01507.a
Fumarase Fum
| CENTER ID: | MymaA.01507.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01507.a.A1.GE30183 | Full length( MymaA.01507.a ) | 1 | 476 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01507.a.A1.PS00842 | Full length( MymaA.01507.a ) | 1 | 476 |
Structures
3QBP
DEPOSITED: 1/13/2011
DETERMINATION: XRay
CLONE: MymaA.01507.a.A1.GE30183
PROTEIN: MymaA.01507.a.A1.PS00842
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127258 |
| PATRIC ID: | fig|216594.6.peg.4696 |
| RefSeq: | YP_001852630.1 |
| UniProt: | B2HT54 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHSAHDDAD NDTEYRIEHD TMGEVRVPAK ALWRAQTQRA VENFPISGRG LERAQIRALG LLKGACAQVN MDLGLLAPEK AEAIIAAAAE IADGQHDDQF PIDVFQTGSG TSSNMNTNEV IASIAGANGV AVHPNDDVNM SQSSNDTFPT ATHIAATEAA VSHLIPALEI LQDALATKAL EWQSVVKSGR THLMDAVPVT LGQEFSGYAR QIEAGIERVR ATLPRLGELA IGGTAVGTGL NAPEGFGVKV VSVLVSQTGL PQLRTAANSF EAQAARDGLV EASGALRTIA VSLTKIANDI RWMGSGPLTG LAEIQLPDLQ PGSSIMPGKV NPVLPEAVTQ VAAQVIGNDA AVAWGGANGA FELNVYIPMM ARNILESFTL LTNVSKLFAQ RCIAGLTANA EHLRELAESS PSIVTPLNSA IGYEEAAAVA KQALKERKTI RQTVIDRGLI GDKLSLEELD RRLDVLAMAK VEATDD
NT Sequence
ATGGCCCACA GCGCCCACGA CGATGCCGAC AACGACACCG AATACCGCAT CGAGCACGAC ACCATGGGCG AGGTCCGGGT GCCGGCAAAG GCACTATGGC GCGCCCAAAC CCAGCGGGCG GTGGAAAACT TCCCCATCTC GGGCCGCGGT CTCGAGCGCG CCCAGATCCG CGCCTTGGGC CTGCTCAAGG GCGCTTGCGC GCAGGTGAAC ATGGACCTCG GGCTGCTGGC ACCGGAGAAG GCCGAAGCGA TCATCGCTGC CGCCGCCGAG ATCGCCGACG GCCAACACGA CGACCAGTTC CCGATCGATG TCTTCCAGAC CGGCTCGGGC ACCAGCTCCA ACATGAACAC CAACGAGGTG ATCGCCAGCA TCGCCGGCGC CAATGGCGTG GCGGTACACC CCAACGACGA CGTCAACATG TCGCAGTCGT CGAACGACAC CTTCCCAACC GCCACTCACA TCGCGGCCAC CGAGGCCGCC GTCAGCCACC TCATCCCCGC CCTGGAGATC TTGCAGGACG CCCTGGCCAC GAAGGCTCTC GAGTGGCAGT CGGTGGTCAA GTCGGGCCGG ACCCACTTGA TGGACGCGGT TCCGGTCACG CTGGGCCAGG AGTTCAGCGG GTATGCCCGC CAGATCGAGG CCGGCATCGA GCGCGTCCGC GCCACGTTGC CCCGACTCGG AGAACTGGCC ATCGGCGGTA CCGCGGTCGG TACCGGACTC AACGCCCCCG AGGGCTTCGG CGTCAAGGTG GTGTCGGTGC TGGTGTCCCA GACCGGGCTG CCCCAATTGC GAACGGCGGC GAACTCTTTC GAAGCCCAAG CCGCACGGGA CGGATTGGTC GAGGCCTCCG GGGCGCTGCG CACCATCGCG GTTTCGCTGA CCAAGATTGC CAACGACATC CGCTGGATGG GCTCCGGTCC GTTGACCGGT TTGGCCGAAA TCCAGCTGCC GGACCTGCAG CCCGGCAGCT CCATCATGCC GGGCAAGGTG AATCCGGTTC TCCCCGAGGC TGTTACGCAG GTCGCGGCGC AGGTGATCGG CAACGACGCG GCGGTGGCCT GGGGCGGCGC CAATGGCGCC TTCGAGCTCA ACGTCTACAT CCCGATGATG GCCCGCAACA TCCTCGAGTC ATTCACGTTG CTGACCAACG TCTCAAAGCT GTTCGCGCAG CGCTGCATTG CCGGGTTGAC CGCCAACGCC GAGCACCTGC GTGAACTCGC GGAGTCCTCA CCGTCGATCG TGACACCGCT GAACTCGGCG ATCGGCTACG AGGAGGCGGC CGCGGTCGCC AAGCAAGCGC TCAAGGAACG CAAGACGATC CGCCAGACCG TCATCGATCG CGGCTTGATC GGCGACAAGC TCTCGCTCGA GGAACTCGAC CGCCGTCTCG ACGTGCTTGC GATGGCCAAG GTCGAAGCGA CCGACGAC
Details for MymaA.01507.a.A1.GE30183
| HARVESTED ON: | 8/15/2010 |
| SEQUENCED ON: | 8/25/2010 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAHSAHDDAD NDTEYRIEHD TMGEVRVPAK ALWRAQTQRA VENFPISGRG LERAQIRALG LLKGACAQVN MDLGLLAPEK AEAIIAAAAE IADGQHDDQF PIDVFQTGSG TSSNMNTNEV IASIAGANGV AVHPNDDVNM SQSSNDTFPT ATHIAATEAA VSHLIPALEI LQDALATKAL EWQSVVKSGR THLMDAVPVT LGQEFSGYAR QIEAGIERVR ATLPRLGELA IGGTAVGTGL NAPEGFGVKV VSVLVSQTGL XQLRTAANSF EAQAARDGLV EASGALRTIA VSLTKIANDI RWMGSGPLTG LAEIQLPDLQ PGSSIMPGKV NPVLPEAVTQ VAAQVIGNDA AVAWGGANGA FELNVYIPMM ARNILESFTL LTNVSKLFAQ RCIAGLTANA EHLRELAESS PSIVTPLNSA IGYEEAAAVA KQALKERKTI RQTVIDRGLI GDKLSLEELD RRLDVLAMAK VEAT
Validated NT Sequence
cggtcgcttc gaccttggcc atcgcaagca cgtcgagacg gcggtcgagt tcctcgagcg agagcttgtc gccgatcaag ccgcgatcga tgacggtctg gcggatcgtc ttgcgttcct tgagcgcttg cttggcgacc gcggccgcct cctcgtagcc gatcgccgag ttcagcggtg tcacgatcga cggtgaggac tccgcgagtt cacgcaggtg ctcggcgttg gcggtcaacc cggcaatgca gcgctgcgcg aacagctttg agacgttggt cagcaacgtg aatgactcga ggatgttgcg ggccatcatc gggatgtaga cgttgagctc gaaggcgcca ttggcgccgc cccaggccac cgccgcgtcg ttgccgatca cctgcgccgc gacctgcgta acagcctcgg ggagaaccgg attcaccttg cccggcatga tggagctgcc gggctgcagg tccggcagct ggatttcggc caaaccggtc aacggaccgg agcccatcca gcggatgtcg ttggcaatct tggtcagcga aaccgcgatg gtgcgcagcg ccccggaggc ctcgaccaat ccgtcccgtg cggcttgggc ttcgaaagag ttcgccgccg ttcgcaattg ggncagcccg gtctgggaca ccagcaccga caccaccttg acgccgaagc cctcgggggc gttgagtccg gtaccgaccg cggtaccgcc gatggccagt tctccgagtc ggggcaacgt ggcgcggacg cgctcgatgc cggcctcgat ctggcgggca tacccgctga actcctggcc cagcgtgacc ggaaccgcgt ccatcaagtg ggtccggccc gacttgacca ccgactgcca ctcgagagcc ttcgtggcca gggcgtcctg caagatctcc agggcgggga tgaggtggct gacggcggcc tcggtggccg cgatgtgagt ggcggttggg aaggtgtcgt tcgacgactg cgacatgttg acgtcgtcgt tggggtgtac cgccacgcca ttggcgccgg cgatgctggc gatcacctcg ttggtgttca tgttggagct ggtgcccgag ccggtctgga agacatcgat cgggaactgg tcgtcgtgtt ggccgtcggc gatctcggcg gcggcagcga tgatcgcttc ggccttctcc ggtgccagca gcccgaggtc catgttcacc tgcgcgcaag cgcccttgag caggcccaag gcgcggatct gggcgcgctc gagaccgcgg cccgagatgg ggaagttttc caccgcccgc tgggtttggg cgcgccatag tgcctttgcc ggcacccgga cctcgcccat ggtgtcgtgc tcgatgcggt attcggtgtc gttgtcggca tcgtcgtggg cgctgtgggc catcgaacca ggaccctggg tctgagcttc cag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHSAHDDA DNDTEYRIEH DTMGEVRVPA KALWRAQTQR AVENFPISGR GLERAQIRAL GLLKGACAQV NMDLGLLAPE KAEAIIAAAA EIADGQHDDQ FPIDVFQTGS GTSSNMNTNE VIASIAGANG VAVHPNDDVN MSQSSNDTFP TATHIAATEA AVSHLIPALE ILQDALATKA LEWQSVVKSG RTHLMDAVPV TLGQEFSGYA RQIEAGIERV RATLPRLGEL AIGGTAVGTG LNAPEGFGVK VVSVLVSQTG LPQLRTAANS FEAQAARDGL VEASGALRTI AVSLTKIAND IRWMGSGPLT GLAEIQLPDL QPGSSIMPGK VNPVLPEAVT QVAAQVIGND AAVAWGGANG AFELNVYIPM MARNILESFT LLTNVSKLFA QRCIAGLTAN AEHLRELAES SPSIVTPLNS AIGYEEAAAV AKQALKERKT IRQTVIDRGL IGDKLSLEEL DRRLDVLAMA KVEATDD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccacagc gcccacgacg atgccgacaa cgacaccgaa taccgcatcg agcacgacac catgggcgag gtccgggtgc cggcaaaggc actatggcgc gcccaaaccc agcgggcggt ggaaaacttc cccatctcgg gccgcggtct cgagcgcgcc cagatccgcg ccttgggcct gctcaagggc gcttgcgcgc aggtgaacat ggacctcggg ctgctggcac cggagaaggc cgaagcgatc atcgctgccg ccgccgagat cgccgacggc caacacgacg accagttccc gatcgatgtc ttccagaccg gctcgggcac cagctccaac atgaacacca acgaggtgat cgccagcatc gccggcgcca atggcgtggc ggtacacccc aacgacgacg tcaacatgtc gcagtcgtcg aacgacacct tcccaaccgc cactcacatc gcggccaccg aggccgccgt cagccacctc atccccgccc tggagatctt gcaggacgcc ctggccacga aggctctcga gtggcagtcg gtggtcaagt cgggccggac ccacttgatg gacgcggttc cggtcacgct gggccaggag ttcagcgggt atgcccgcca gatcgaggcc ggcatcgagc gcgtccgcgc cacgttgccc cgactcggag aactggccat cggcggtacc gcggtcggta ccggactcaa cgcccccgag ggcttcggcg tcaaggtggt gtcggtgctg gtgtcccaga ccgggctgcc ccaattgcga acggcggcga actctttcga agcccaagcc gcacgggacg gattggtcga ggcctccggg gcgctgcgca ccatcgcggt ttcgctgacc aagattgcca acgacatccg ctggatgggc tccggtccgt tgaccggttt ggccgaaatc cagctgccgg acctgcagcc cggcagctcc atcatgccgg gcaaggtgaa tccggttctc cccgaggctg ttacgcaggt cgcggcgcag gtgatcggca acgacgcggc ggtggcctgg ggcggcgcca atggcgcctt cgagctcaac gtctacatcc cgatgatggc ccgcaacatc ctcgagtcat tcacgttgct gaccaacgtc tcaaagctgt tcgcgcagcg ctgcattgcc gggttgaccg ccaacgccga gcacctgcgt gaactcgcgg agtcctcacc gtcgatcgtg acaccgctga actcggcgat cggctacgag gaggcggccg cggtcgccaa gcaagcgctc aaggaacgca agacgatccg ccagaccgtc atcgatcgcg gcttgatcgg cgacaagctc tcgctcgagg aactcgaccg ccgtctcgac gtgcttgcga tggccaaggt cgaagcgacc gacgacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.01507.a.A1.PS00842
| PURIFICATION DATe: | 11/1/2010 |
| CONCENTRATION: | 51.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHSAHDDAD NDTEYRIEHD TMGEVRVPAK ALWRAQTQRA VENFPISGRG LERAQIRALG LLKGACAQVN MDLGLLAPEK AEAIIAAAAE IADGQHDDQF PIDVFQTGSG TSSNMNTNEV IASIAGANGV AVHPNDDVNM SQSSNDTFPT ATHIAATEAA VSHLIPALEI LQDALATKAL EWQSVVKSGR THLMDAVPVT LGQEFSGYAR QIEAGIERVR ATLPRLGELA IGGTAVGTGL NAPEGFGVKV VSVLVSQTGL XQLRTAANSF EAQAARDGLV EASGALRTIA VSLTKIANDI RWMGSGPLTG LAEIQLPDLQ PGSSIMPGKV NPVLPEAVTQ VAAQVIGNDA AVAWGGANGA FELNVYIPMM ARNILESFTL LTNVSKLFAQ RCIAGLTANA EHLRELAESS PSIVTPLNSA IGYEEAAAVA KQALKERKTI RQTVIDRGLI GDKLSLEELD RRLDVLAMAK VEAT
Validated NT Sequence
cggtcgcttc gaccttggcc atcgcaagca cgtcgagacg gcggtcgagt tcctcgagcg agagcttgtc gccgatcaag ccgcgatcga tgacggtctg gcggatcgtc ttgcgttcct tgagcgcttg cttggcgacc gcggccgcct cctcgtagcc gatcgccgag ttcagcggtg tcacgatcga cggtgaggac tccgcgagtt cacgcaggtg ctcggcgttg gcggtcaacc cggcaatgca gcgctgcgcg aacagctttg agacgttggt cagcaacgtg aatgactcga ggatgttgcg ggccatcatc gggatgtaga cgttgagctc gaaggcgcca ttggcgccgc cccaggccac cgccgcgtcg ttgccgatca cctgcgccgc gacctgcgta acagcctcgg ggagaaccgg attcaccttg cccggcatga tggagctgcc gggctgcagg tccggcagct ggatttcggc caaaccggtc aacggaccgg agcccatcca gcggatgtcg ttggcaatct tggtcagcga aaccgcgatg gtgcgcagcg ccccggaggc ctcgaccaat ccgtcccgtg cggcttgggc ttcgaaagag ttcgccgccg ttcgcaattg ggncagcccg gtctgggaca ccagcaccga caccaccttg acgccgaagc cctcgggggc gttgagtccg gtaccgaccg cggtaccgcc gatggccagt tctccgagtc ggggcaacgt ggcgcggacg cgctcgatgc cggcctcgat ctggcgggca tacccgctga actcctggcc cagcgtgacc ggaaccgcgt ccatcaagtg ggtccggccc gacttgacca ccgactgcca ctcgagagcc ttcgtggcca gggcgtcctg caagatctcc agggcgggga tgaggtggct gacggcggcc tcggtggccg cgatgtgagt ggcggttggg aaggtgtcgt tcgacgactg cgacatgttg acgtcgtcgt tggggtgtac cgccacgcca ttggcgccgg cgatgctggc gatcacctcg ttggtgttca tgttggagct ggtgcccgag ccggtctgga agacatcgat cgggaactgg tcgtcgtgtt ggccgtcggc gatctcggcg gcggcagcga tgatcgcttc ggccttctcc ggtgccagca gcccgaggtc catgttcacc tgcgcgcaag cgcccttgag caggcccaag gcgcggatct gggcgcgctc gagaccgcgg cccgagatgg ggaagttttc caccgcccgc tgggtttggg cgcgccatag tgcctttgcc ggcacccgga cctcgcccat ggtgtcgtgc tcgatgcggt attcggtgtc gttgtcggca tcgtcgtggg cgctgtgggc catcgaacca ggaccctggg tctgagcttc cag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHSAHDDA DNDTEYRIEH DTMGEVRVPA KALWRAQTQR AVENFPISGR GLERAQIRAL GLLKGACAQV NMDLGLLAPE KAEAIIAAAA EIADGQHDDQ FPIDVFQTGS GTSSNMNTNE VIASIAGANG VAVHPNDDVN MSQSSNDTFP TATHIAATEA AVSHLIPALE ILQDALATKA LEWQSVVKSG RTHLMDAVPV TLGQEFSGYA RQIEAGIERV RATLPRLGEL AIGGTAVGTG LNAPEGFGVK VVSVLVSQTG LPQLRTAANS FEAQAARDGL VEASGALRTI AVSLTKIAND IRWMGSGPLT GLAEIQLPDL QPGSSIMPGK VNPVLPEAVT QVAAQVIGND AAVAWGGANG AFELNVYIPM MARNILESFT LLTNVSKLFA QRCIAGLTAN AEHLRELAES SPSIVTPLNS AIGYEEAAAV AKQALKERKT IRQTVIDRGL IGDKLSLEEL DRRLDVLAMA KVEATDD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCCACAGC GCCCACGACG ATGCCGACAA CGACACCGAA TACCGCATCG AGCACGACAC CATGGGCGAG GTCCGGGTGC CGGCAAAGGC ACTATGGCGC GCCCAAACCC AGCGGGCGGT GGAAAACTTC CCCATCTCGG GCCGCGGTCT CGAGCGCGCC CAGATCCGCG CCTTGGGCCT GCTCAAGGGC GCTTGCGCGC AGGTGAACAT GGACCTCGGG CTGCTGGCAC CGGAGAAGGC CGAAGCGATC ATCGCTGCCG CCGCCGAGAT CGCCGACGGC CAACACGACG ACCAGTTCCC GATCGATGTC TTCCAGACCG GCTCGGGCAC CAGCTCCAAC ATGAACACCA ACGAGGTGAT CGCCAGCATC GCCGGCGCCA ATGGCGTGGC GGTACACCCC AACGACGACG TCAACATGTC GCAGTCGTCG AACGACACCT TCCCAACCGC CACTCACATC GCGGCCACCG AGGCCGCCGT CAGCCACCTC ATCCCCGCCC TGGAGATCTT GCAGGACGCC CTGGCCACGA AGGCTCTCGA GTGGCAGTCG GTGGTCAAGT CGGGCCGGAC CCACTTGATG GACGCGGTTC CGGTCACGCT GGGCCAGGAG TTCAGCGGGT ATGCCCGCCA GATCGAGGCC GGCATCGAGC GCGTCCGCGC CACGTTGCCC CGACTCGGAG AACTGGCCAT CGGCGGTACC GCGGTCGGTA CCGGACTCAA CGCCCCCGAG GGCTTCGGCG TCAAGGTGGT GTCGGTGCTG GTGTCCCAGA CCGGGCTGCC CCAATTGCGA ACGGCGGCGA ACTCTTTCGA AGCCCAAGCC GCACGGGACG GATTGGTCGA GGCCTCCGGG GCGCTGCGCA CCATCGCGGT TTCGCTGACC AAGATTGCCA ACGACATCCG CTGGATGGGC TCCGGTCCGT TGACCGGTTT GGCCGAAATC CAGCTGCCGG ACCTGCAGCC CGGCAGCTCC ATCATGCCGG GCAAGGTGAA TCCGGTTCTC CCCGAGGCTG TTACGCAGGT CGCGGCGCAG GTGATCGGCA ACGACGCGGC GGTGGCCTGG GGCGGCGCCA ATGGCGCCTT CGAGCTCAAC GTCTACATCC CGATGATGGC CCGCAACATC CTCGAGTCAT TCACGTTGCT GACCAACGTC TCAAAGCTGT TCGCGCAGCG CTGCATTGCC GGGTTGACCG CCAACGCCGA GCACCTGCGT GAACTCGCGG AGTCCTCACC GTCGATCGTG ACACCGCTGA ACTCGGCGAT CGGCTACGAG GAGGCGGCCG CGGTCGCCAA GCAAGCGCTC AAGGAACGCA AGACGATCCG CCAGACCGTC ATCGATCGCG GCTTGATCGG CGACAAGCTC TCGCTCGAGG AACTCGACCG CCGTCTCGAC GTGCTTGCGA TGGCCAAGGT CGAAGCGACC GACGACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA