MypaA.01507.a

Fum

CENTER ID: MypaA.01507.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01507.a.A1.GE29607 Full length( MypaA.01507.a ) 1 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2861
RefSeq: NP_961627.1
UniProt: Q73WG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATDDGDTDY RIERDTMGEV RVPAKALWRA QTQRAVENFP ISGRGLERTQ IRALGLLKGA CAQVNKDLGL LAPEKADAII AAAAEIADGR HDDQFPIDVF QTGSGTSSNM NTNEVIASIA AANGVTVHPN DDVNMSQSSN DTFPTATHIA ATEAAVRHLI PALQVLHDAL ASKAREWHTV VKSGRTHLMD AVPVTLGQEF SGYARQIEAG IERVRATLPR LGELAIGGTA VGTGLNAPDG FGAKVVETLI ASTGLSELRT ATNSFEAQAA RDGLVEASGA LRTIAVSLTK IANDIRWMGS GPLTGLAEIA LPDLQPGSSI MPGKVNPVIP EAVTQVAAQV IGNDAAVAVG GLSGAFELNV YIPMMARNIL ESFKLLTNVS RLFAERCISG LSANVEHLRE LAESSPSIVT PLNSAIGYEE AAAVAKQALK ERKTIRQTVI DRGLIGDKLS LEELDRRLDV LAMAKVKPQD
NT Sequence
ATGGCCACTG ACGACGGCGA CACCGACTAC CGCATCGAGC GCGACACCAT GGGCGAGGTC CGGGTACCCG CAAAAGCGTT GTGGCGCGCG CAAACCCAGC GCGCGGTCGA GAACTTCCCG ATCTCGGGCC GGGGCCTGGA ACGCACCCAG ATCCGCGCCC TGGGCCTGCT GAAGGGCGCC TGCGCGCAGG TCAACAAGGA TCTGGGGCTG CTGGCGCCGG AGAAGGCCGA CGCGATCATC GCCGCGGCCG CCGAGATCGC CGACGGTCGC CACGACGACC AGTTTCCCAT CGACGTCTTC CAGACCGGCT CGGGCACCAG CTCGAACATG AACACCAACG AGGTGATCGC GTCCATCGCG GCCGCCAACG GGGTGACCGT GCATCCTAAC GACGACGTCA ACATGTCGCA GTCGTCGAAC GACACCTTCC CCACCGCAAC CCACATCGCG GCCACGGAAG CCGCAGTGCG CCATCTGATT CCGGCGCTGC AGGTGCTGCA CGACGCCCTG GCGAGCAAGG CCCGCGAGTG GCACACCGTG GTCAAGTCGG GCCGCACCCA CCTGATGGAC GCCGTGCCGG TGACACTCGG CCAGGAGTTC AGCGGCTACG CCCGCCAGAT CGAGGCCGGC ATCGAACGGG TGCGGGCCAC GCTGCCCCGG CTGGGTGAGC TGGCGATCGG CGGGACCGCG GTGGGCACCG GCCTCAACGC TCCCGACGGC TTCGGCGCCA AGGTGGTCGA GACGCTGATC GCCTCCACCG GATTGAGCGA ATTGCGCACG GCGACAAACT CGTTCGAGGC CCAGGCGGCG CGCGACGGGC TGGTCGAGGC CTCCGGCGCG CTGCGCACCA TCGCGGTGTC GCTGACCAAG ATCGCCAACG ACATCCGCTG GATGGGCTCG GGGCCGCTGA CCGGCCTGGC CGAGATCGCC CTTCCCGATC TGCAGCCGGG CAGCTCGATC ATGCCGGGCA AGGTTAATCC GGTTATCCCC GAAGCGGTTA CGCAGGTCGC CGCGCAGGTG ATCGGCAACG ACGCGGCGGT GGCCGTCGGC GGCTTGTCGG GCGCCTTCGA GCTCAACGTG TACATCCCGA TGATGGCCCG CAACATCCTG GAGTCGTTCA AGCTGCTGAC CAACGTGTCG AGGCTGTTCG CCGAGCGCTG CATCAGCGGG CTGAGCGCCA ACGTGGAGCA CCTGCGCGAG CTCGCCGAAT CCTCACCGTC CATCGTGACG CCGCTGAACT CGGCGATCGG CTACGAGGAG GCGGCGGCGG TGGCCAAGCA GGCGCTCAAG GAGCGCAAGA CGATTCGGCA GACCGTCATC GACCGCGGGC TGATCGGCGA CAAGCTGTCG CTCGAGGAGC TCGACCGCCG GCTCGACGTG CTGGCCATGG CCAAGGTCAA GCCGCAGGAC
Details for MypaA.01507.a.A1.GE29607
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 51kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 79
Validated AA Sequence
MDGEDSASSR RCSTLALSPL MQRSANSLDT LVSSLNDSRM LRAIIGMYTL SSKAPDKPPT ATAASLPITC AATCVTASGI TGLTLPGMIE LPGCRSGRAI SARPVSGPEP IQRMSLAILV SDTAMVRSAP XASTSPSRAA WASNEFVAVR NSLNPVEAIS VSTTLAPKPS GALRPGAHRG PADRQLTQPG QRGPHPFDAG LDLAGVAAEL LAECHRHGVH QVGAARLDHG VPLAGLARQG VVQHLQRRNQ MAHCGFRGRD VGCGGEGVVR RLRHVDVVVR MHGHPVGGRD GRDHLVGVHV RAGARAGLED VDGKLVVVAT VGDLGGRGDD RVGLLRRQQP QILVDLRAGA LQQAQGADLG AFQAPARDRE VLDRALGLRA PQRFCGYPDL AH
Validated NT Sequence
cggcttgacc ttggccatgg ccagcacgtc gagccggcgg tcgagctcct cgagcgacag cttgtcgccg atcagcccgc ggtcgatgac ggtctgccga atcgtcttgc gctccttgag cgcctgcttg gccaccgccg ccgcctcctc gtagccgatc gccgagttca gcggcgtcac gatggacggt gaggattcgg cgagctcgcg caggtgctcc acgttggcgc tcagcccgct gatgcagcgc tcggcgaaca gcctcgacac gttggtcagc agcttgaacg actccaggat gttgcgggcc atcatcggga tgtacacgtt gagctcgaag gcgcccgaca agccgccgac ggccaccgcc gcgtcgttgc cgatcacctg cgcggcgacc tgcgtaaccg cttcggggat aaccggatta accttgcccg gcatgatcga gctgcccggc tgcagatcgg gaagggcgat ctcggccagg ccggtcagcg gccccgagcc catccagcgg atgtcgttgg cgatcttggt cagcgacacc gcgatggtgc gcagcgcgcc ggangcctcg accagcccgt cgcgcgccgc ctgggcctcg aacgagtttg tcgccgtgcg caattcgctc aatccggtgg aggcgatcag cgtctcgacc accttggcgc cgaagccgtc gggagcgttg aggccnggtg cccaccgcgg tcccgccgat cgccagctca cccagccggg gcagcgtggc ccgcacccgt tcgatgccgg cctcgatctg gcgggcgtag ccgctgaact cctggccgag tgtcaccggc acggcgtcca tcaggtgggt gcggcccgac ttgaccacgg tgtgccactc gcgggccttg ctcgccaggg cgtcgtgcag cacctgcagc gccggaatca gatggcgcac tgcggcttcc gtggccgcga tgtgggttgc ggtggggaag gtgtcgttcg acgactgcga catgttgacg tcgtcgttag gatgcacggt caccccgttg gcggccgcga tggacgcgat cacctcgttg gtgttcatgt tcgagctggt gcccgagccg gtctggaaga cgtcgatggg aaactggtcg tcgtggcgac cgtcggcgat ctcggcggcc gcggcgatga tcgcgtcggc cttctccggc gccagcagcc ccagatcctt gttgacctgc gcgcaggcgc ccttcagcag gcccagggcg cggatctggg tgcgttccag gccccggccc gagatcggga agttctcgac cgcgcgctgg gtttgcgcgc gccacaacgc ttttgcgggt acccggacct cgcccatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATDDGDTD YRIERDTMGE VRVPAKALWR AQTQRAVENF PISGRGLERT QIRALGLLKG ACAQVNKDLG LLAPEKADAI IAAAAEIADG RHDDQFPIDV FQTGSGTSSN MNTNEVIASI AAANGVTVHP NDDVNMSQSS NDTFPTATHI AATEAAVRHL IPALQVLHDA LASKAREWHT VVKSGRTHLM DAVPVTLGQE FSGYARQIEA GIERVRATLP RLGELAIGGT AVGTGLNAPD GFGAKVVETL IASTGLSELR TATNSFEAQA ARDGLVEASG ALRTIAVSLT KIANDIRWMG SGPLTGLAEI ALPDLQPGSS IMPGKVNPVI PEAVTQVAAQ VIGNDAAVAV GGLSGAFELN VYIPMMARNI LESFKLLTNV SRLFAERCIS GLSANVEHLR ELAESSPSIV TPLNSAIGYE EAAAVAKQAL KERKTIRQTV IDRGLIGDKL SLEELDRRLD VLAMAKVKPQ D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccactgac gacggcgaca ccgactaccg catcgagcgc gacaccatgg gcgaggtccg ggtacccgca aaagcgttgt ggcgcgcgca aacccagcgc gcggtcgaga acttcccgat ctcgggccgg ggcctggaac gcacccagat ccgcgccctg ggcctgctga agggcgcctg cgcgcaggtc aacaaggatc tggggctgct ggcgccggag aaggccgacg cgatcatcgc cgcggccgcc gagatcgccg acggtcgcca cgacgaccag tttcccatcg acgtcttcca gaccggctcg ggcaccagct cgaacatgaa caccaacgag gtgatcgcgt ccatcgcggc cgccaacggg gtgaccgtgc atcctaacga cgacgtcaac atgtcgcagt cgtcgaacga caccttcccc accgcaaccc acatcgcggc cacggaagcc gcagtgcgcc atctgattcc ggcgctgcag gtgctgcacg acgccctggc gagcaaggcc cgcgagtggc acaccgtggt caagtcgggc cgcacccacc tgatggacgc cgtgccggtg acactcggcc aggagttcag cggctacgcc cgccagatcg aggccggcat cgaacgggtg cgggccacgc tgccccggct gggtgagctg gcgatcggcg ggaccgcggt gggcaccggc ctcaacgctc ccgacggctt cggcgccaag gtggtcgaga cgctgatcgc ctccaccgga ttgagcgaat tgcgcacggc gacaaactcg ttcgaggccc aggcggcgcg cgacgggctg gtcgaggcct ccggcgcgct gcgcaccatc gcggtgtcgc tgaccaagat cgccaacgac atccgctgga tgggctcggg gccgctgacc ggcctggccg agatcgccct tcccgatctg cagccgggca gctcgatcat gccgggcaag gttaatccgg ttatccccga agcggttacg caggtcgccg cgcaggtgat cggcaacgac gcggcggtgg ccgtcggcgg cttgtcgggc gccttcgagc tcaacgtgta catcccgatg atggcccgca acatcctgga gtcgttcaag ctgctgacca acgtgtcgag gctgttcgcc gagcgctgca tcagcgggct gagcgccaac gtggagcacc tgcgcgagct cgccgaatcc tcaccgtcca tcgtgacgcc gctgaactcg gcgatcggct acgaggaggc ggcggcggtg gccaagcagg cgctcaagga gcgcaagacg attcggcaga ccgtcatcga ccgcgggctg atcggcgaca agctgtcgct cgaggagctc gaccgccggc tcgacgtgct ggccatggcc aaggtcaagc cgcaggacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa