MysmA.01507.a
Fumarate hydratase class II
| CENTER ID: | MysmA.01507.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01507.a.A1.PS00681 | Full length( MysmA.01507.a ) | 1 | 468 | ||
| MysmA.01507.a.A1.PS00682 | Full length( MysmA.01507.a ) | 1 | 468 |
Structures
3RD8
DEPOSITED: 4/1/2011
DETERMINATION: XRay
CLONE: MysmA.01507.a.A1.GE28939
PROTEIN: MysmA.01507.a.A1.PS00681
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127258 |
| PATRIC ID: | fig|246196.19.peg.5111 |
| RefSeq: | YP_889486.1 |
| UniProt: | A0R2U8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADTDVEYRI EHDTMGEVRV PKDALWRAQT QRAVENFPIS FRGLERTQIR ALGLLKAACA QVNKDLGLLD PEKADAIIAA AGEIAEGKHD DQFPIDVFQT GSGTSSNMNT NEVIASIAAA NGVTVHPNDH VNMSQSSNDT FPTATHIAAT EAAVRHLIPA LEVLHASLAA KAKQWRTVVK SGRTHLMDAV PVTLGQEFGG YARQIEAGIE RVKATLPRLG ELAIGGTAVG TGLNAPEGFG AKVVEVLVNE TGLAELRTAV DSFEAQAARD GLVEASGALR TIAVSLTKIA NDIRWMGSGP LTGLAEIQLP DLQPGSSIMP GKVNPVLPEA VTQVACQVVG NDAAIAFGGA SGAFELNVYI PMMARNLLES FTLLSNVSRL FAERCIDGLV ANEERLRELA ESSPSIVTPL NSAIGYEEAA KVAKQALAEK KTIRQTVIDR GLIGDKLSLE ELDRRLDVLA MARVKDGE
NT Sequence
ATGGCCGACA CCGACGTCGA ATACCGCATC GAACACGACA CCATGGGTGA AGTCCGGGTA CCCAAGGACG CCCTGTGGCG CGCGCAGACC CAGCGCGCGG TCGAGAACTT CCCCATCTCG TTCCGCGGCC TCGAGCGCAC GCAGATCCGC GCGCTCGGTC TCCTGAAGGC CGCGTGCGCG CAGGTCAACA AGGACCTGGG CCTGCTGGAC CCCGAGAAGG CCGACGCGAT CATCGCCGCC GCGGGTGAGA TCGCCGAGGG CAAGCACGAC GACCAGTTCC CCATCGACGT GTTCCAGACC GGCTCGGGCA CGAGCTCGAA CATGAACACC AACGAGGTGA TCGCGTCGAT CGCGGCAGCC AACGGTGTGA CGGTGCACCC GAACGACCAC GTGAACATGT CGCAGAGCTC CAACGACACC TTCCCCACCG CGACCCACAT CGCGGCCACG GAAGCCGCTG TGCGCCATCT GATCCCGGCG CTGGAGGTCC TGCACGCATC GCTGGCCGCC AAGGCCAAGC AGTGGCGCAC CGTCGTCAAG TCCGGCCGCA CGCACCTCAT GGACGCCGTG CCCGTCACGC TCGGGCAGGA GTTCGGCGGC TATGCGCGCC AGATCGAGGC AGGCATCGAA CGCGTCAAGG CCACGCTGCC ACGTCTCGGT GAACTCGCGA TCGGCGGCAC CGCCGTCGGC ACCGGCCTCA ACGCGCCGGA AGGCTTCGGC GCCAAGGTCG TCGAGGTGCT CGTCAACGAG ACCGGCCTGG CCGAGTTGCG CACCGCCGTC GACTCGTTCG AGGCGCAGGC CGCGCGCGAC GGGCTGGTCG AGGCCTCCGG TGCGCTGCGC ACCATCGCGG TGTCGCTCAC CAAGATCGCC AACGACATCC GCTGGATGGG CTCGGGCCCG CTGACCGGCC TGGCCGAGAT CCAGCTGCCG GACCTGCAGC CGGGCAGCTC GATCATGCCG GGCAAGGTCA ACCCCGTCCT GCCCGAGGCC GTCACGCAGG TGGCCTGCCA GGTGGTCGGC AACGACGCGG CGATCGCGTT CGGCGGTGCG TCGGGTGCCT TCGAGCTCAA CGTGTACATC CCGATGATGG CGCGCAACCT GCTCGAGTCG TTCACGTTGC TGTCCAACGT GTCCCGGCTG TTCGCCGAGC GCTGCATCGA CGGCCTGGTG GCCAACGAGG AGCGCCTGCG GGAGCTCGCC GAGTCCTCGC CGTCGATCGT GACGCCGCTG AACTCGGCGA TCGGTTACGA AGAGGCCGCC AAGGTCGCCA AGCAGGCTCT CGCGGAGAAG AAGACCATCC GCCAGACCGT GATCGACCGT GGCCTCATCG GCGACAAGCT GAGCCTCGAG GAACTCGACC GTCGTCTCGA CGTGCTCGCC ATGGCGCGGG TCAAGGACGG CGAG
Details for MysmA.01507.a.A1.PS00681
| PURIFICATION DATe: | 7/21/2010 |
| CONCENTRATION: | 67.55mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 57 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDAVPVTLGQ EFGGYARQIE AGIERVKATL PRLGELAIGG TAVGTGLNAP EGFGAKVVEV LVNETGLAEL RTAVDSFEAQ AARDGLVEAS GALRTIAVSL TKIANDIRWM GSGPLTGLAE IQLPDLQPGS SIMPGKVNPV LPEAVTQVAC QVVGNDAAIA FGGASGAFEL NVYIPMMARN LLESFTLLSN VSRLFAERCI DGLVANEERL RELAESSPSI VTPLNSAIGY EEAAKVAKQA LAEKKTIRQT VIDRGLIGDK LSLEELDRRL DVLAMARVKD GE
Validated NT Sequence
ttcgngcttt gttngcagcc ggntcctcga gaagcttggc tgcagaactt gttcgtgctg tttattactc gccgtccttg acccgcgcca tggcgagcac gtcgagacga cggtcgagtt cctcgaggct cagcttgtcg ccgatgaggc cacggtcgat cacggtctgg cggatggtct tcttctccgc gagagcctgc ttggcgacct tggcggcctc ttcgtaaccg atcgccgagt tcagcggcgt cacgatcgac ggcgaggact cggcgagctc ccgcaggcgc tcctcgttgg ccaccaggcc gtcgatgcag cgctcggcga acagccggga cacgttggac agcaacgtga acgactcgag caggttgcgc gccatcatcg ggatgtacac gttgagctcg aaggcacccg acgcaccgcc gaacgcgatc gccgcgtcgt tgccgaccac ctggcaggcc acctgcgtga cggcctcggg caggacgggg ttgaccttgc ccggcatgat cgagctgccc ggctgcaggt ccggcagctg gatctcggcc aggccggtca gcgggcccga gcccatccag cggatgtcgt tggcgatctt ggtgagcgac accgcgatgg tgcgcagcgc accggaggcc tcgaccagcc cgtcgcgcgc ggcctgcgcc tcgaacgagt cgacggcggt gcgcaactcg gccaggccgg tctcgttgac gagcacctcg acgaccttgg cgccgaagcc ttccggcgcg ttgaggccgg tgccgacggc ggtgccgccg atcgcgagtt caccgagacg tggcagcgtg gccttgacgc gttcgatgcc tgcctcgatc tggcgcgcat agccgccgaa ctcctgcccg agcgtgacgg gcacggcgtc catgaggtgc gtgcggccgg acttgacgac ggtgcgccac tgcttgnnnt tggnggccag cgatgcgtgc aggacctncn gcgccggnnn cagatggcgc ncancngctt ncgnnnnnnc gatgtggntc gcggngggga angngtcnnn gganctctgc nacntnnnca cnncnnnnng nnnnannntc nnnnncnnnn nnncnnnatc nannnnntcn cnnnnngnnn nnnnnnnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnngn nnnncnnnnn nnnnnccnnn nnnnnnnntn nnnccnnnnn na
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADTDVEYR IEHDTMGEVR VPKDALWRAQ TQRAVENFPI SFRGLERTQI RALGLLKAAC AQVNKDLGLL DPEKADAIIA AAGEIAEGKH DDQFPIDVFQ TGSGTSSNMN TNEVIASIAA ANGVTVHPND HVNMSQSSND TFPTATHIAA TEAAVRHLIP ALEVLHASLA AKAKQWRTVV KSGRTHLMDA VPVTLGQEFG GYARQIEAGI ERVKATLPRL GELAIGGTAV GTGLNAPEGF GAKVVEVLVN ETGLAELRTA VDSFEAQAAR DGLVEASGAL RTIAVSLTKI ANDIRWMGSG PLTGLAEIQL PDLQPGSSIM PGKVNPVLPE AVTQVACQVV GNDAAIAFGG ASGAFELNVY IPMMARNLLE SFTLLSNVSR LFAERCIDGL VANEERLREL AESSPSIVTP LNSAIGYEEA AKVAKQALAE KKTIRQTVID RGLIGDKLSL EELDRRLDVL AMARVKDGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGACACC GACGTCGAAT ACCGCATCGA ACACGACACC ATGGGTGAAG TCCGGGTACC CAAGGACGCC CTGTGGCGCG CGCAGACCCA GCGCGCGGTC GAGAACTTCC CCATCTCGTT CCGCGGCCTC GAGCGCACGC AGATCCGCGC GCTCGGTCTC CTGAAGGCCG CGTGCGCGCA GGTCAACAAG GACCTGGGCC TGCTGGACCC CGAGAAGGCC GACGCGATCA TCGCCGCCGC GGGTGAGATC GCCGAGGGCA AGCACGACGA CCAGTTCCCC ATCGACGTGT TCCAGACCGG CTCGGGCACG AGCTCGAACA TGAACACCAA CGAGGTGATC GCGTCGATCG CGGCAGCCAA CGGTGTGACG GTGCACCCGA ACGACCACGT GAACATGTCG CAGAGCTCCA ACGACACCTT CCCCACCGCG ACCCACATCG CGGCCACGGA AGCCGCTGTG CGCCATCTGA TCCCGGCGCT GGAGGTCCTG CACGCATCGC TGGCCGCCAA GGCCAAGCAG TGGCGCACCG TCGTCAAGTC CGGCCGCACG CACCTCATGG ACGCCGTGCC CGTCACGCTC GGGCAGGAGT TCGGCGGCTA TGCGCGCCAG ATCGAGGCAG GCATCGAACG CGTCAAGGCC ACGCTGCCAC GTCTCGGTGA ACTCGCGATC GGCGGCACCG CCGTCGGCAC CGGCCTCAAC GCGCCGGAAG GCTTCGGCGC CAAGGTCGTC GAGGTGCTCG TCAACGAGAC CGGCCTGGCC GAGTTGCGCA CCGCCGTCGA CTCGTTCGAG GCGCAGGCCG CGCGCGACGG GCTGGTCGAG GCCTCCGGTG CGCTGCGCAC CATCGCGGTG TCGCTCACCA AGATCGCCAA CGACATCCGC TGGATGGGCT CGGGCCCGCT GACCGGCCTG GCCGAGATCC AGCTGCCGGA CCTGCAGCCG GGCAGCTCGA TCATGCCGGG CAAGGTCAAC CCCGTCCTGC CCGAGGCCGT CACGCAGGTG GCCTGCCAGG TGGTCGGCAA CGACGCGGCG ATCGCGTTCG GCGGTGCGTC GGGTGCCTTC GAGCTCAACG TGTACATCCC GATGATGGCG CGCAACCTGC TCGAGTCGTT CACGTTGCTG TCCAACGTGT CCCGGCTGTT CGCCGAGCGC TGCATCGACG GCCTGGTGGC CAACGAGGAG CGCCTGCGGG AGCTCGCCGA GTCCTCGCCG TCGATCGTGA CGCCGCTGAA CTCGGCGATC GGTTACGAAG AGGCCGCCAA GGTCGCCAAG CAGGCTCTCG CGGAGAAGAA GACCATCCGC CAGACCGTGA TCGACCGTGG CCTCATCGGC GACAAGCTGA GCCTCGAGGA ACTCGACCGT CGTCTCGACG TGCTCGCCAT GGCGCGGGTC AAGGACGGCG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for MysmA.01507.a.A1.PS00682
| PURIFICATION DATe: | 7/21/2010 |
| CONCENTRATION: | 57.76mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 57 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDAVPVTLGQ EFGGYARQIE AGIERVKATL PRLGELAIGG TAVGTGLNAP EGFGAKVVEV LVNETGLAEL RTAVDSFEAQ AARDGLVEAS GALRTIAVSL TKIANDIRWM GSGPLTGLAE IQLPDLQPGS SIMPGKVNPV LPEAVTQVAC QVVGNDAAIA FGGASGAFEL NVYIPMMARN LLESFTLLSN VSRLFAERCI DGLVANEERL RELAESSPSI VTPLNSAIGY EEAAKVAKQA LAEKKTIRQT VIDRGLIGDK LSLEELDRRL DVLAMARVKD GE
Validated NT Sequence
ttcgngcttt gttngcagcc ggntcctcga gaagcttggc tgcagaactt gttcgtgctg tttattactc gccgtccttg acccgcgcca tggcgagcac gtcgagacga cggtcgagtt cctcgaggct cagcttgtcg ccgatgaggc cacggtcgat cacggtctgg cggatggtct tcttctccgc gagagcctgc ttggcgacct tggcggcctc ttcgtaaccg atcgccgagt tcagcggcgt cacgatcgac ggcgaggact cggcgagctc ccgcaggcgc tcctcgttgg ccaccaggcc gtcgatgcag cgctcggcga acagccggga cacgttggac agcaacgtga acgactcgag caggttgcgc gccatcatcg ggatgtacac gttgagctcg aaggcacccg acgcaccgcc gaacgcgatc gccgcgtcgt tgccgaccac ctggcaggcc acctgcgtga cggcctcggg caggacgggg ttgaccttgc ccggcatgat cgagctgccc ggctgcaggt ccggcagctg gatctcggcc aggccggtca gcgggcccga gcccatccag cggatgtcgt tggcgatctt ggtgagcgac accgcgatgg tgcgcagcgc accggaggcc tcgaccagcc cgtcgcgcgc ggcctgcgcc tcgaacgagt cgacggcggt gcgcaactcg gccaggccgg tctcgttgac gagcacctcg acgaccttgg cgccgaagcc ttccggcgcg ttgaggccgg tgccgacggc ggtgccgccg atcgcgagtt caccgagacg tggcagcgtg gccttgacgc gttcgatgcc tgcctcgatc tggcgcgcat agccgccgaa ctcctgcccg agcgtgacgg gcacggcgtc catgaggtgc gtgcggccgg acttgacgac ggtgcgccac tgcttgnnnt tggnggccag cgatgcgtgc aggacctncn gcgccggnnn cagatggcgc ncancngctt ncgnnnnnnc gatgtggntc gcggngggga angngtcnnn gganctctgc nacntnnnca cnncnnnnng nnnnannntc nnnnncnnnn nnncnnnatc nannnnntcn cnnnnngnnn nnnnnnnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnngn nnnncnnnnn nnnnnccnnn nnnnnnnntn nnnccnnnnn na
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADTDVEYR IEHDTMGEVR VPKDALWRAQ TQRAVENFPI SFRGLERTQI RALGLLKAAC AQVNKDLGLL DPEKADAIIA AAGEIAEGKH DDQFPIDVFQ TGSGTSSNMN TNEVIASIAA ANGVTVHPND HVNMSQSSND TFPTATHIAA TEAAVRHLIP ALEVLHASLA AKAKQWRTVV KSGRTHLMDA VPVTLGQEFG GYARQIEAGI ERVKATLPRL GELAIGGTAV GTGLNAPEGF GAKVVEVLVN ETGLAELRTA VDSFEAQAAR DGLVEASGAL RTIAVSLTKI ANDIRWMGSG PLTGLAEIQL PDLQPGSSIM PGKVNPVLPE AVTQVACQVV GNDAAIAFGG ASGAFELNVY IPMMARNLLE SFTLLSNVSR LFAERCIDGL VANEERLREL AESSPSIVTP LNSAIGYEEA AKVAKQALAE KKTIRQTVID RGLIGDKLSL EELDRRLDVL AMARVKDGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGACACC GACGTCGAAT ACCGCATCGA ACACGACACC ATGGGTGAAG TCCGGGTACC CAAGGACGCC CTGTGGCGCG CGCAGACCCA GCGCGCGGTC GAGAACTTCC CCATCTCGTT CCGCGGCCTC GAGCGCACGC AGATCCGCGC GCTCGGTCTC CTGAAGGCCG CGTGCGCGCA GGTCAACAAG GACCTGGGCC TGCTGGACCC CGAGAAGGCC GACGCGATCA TCGCCGCCGC GGGTGAGATC GCCGAGGGCA AGCACGACGA CCAGTTCCCC ATCGACGTGT TCCAGACCGG CTCGGGCACG AGCTCGAACA TGAACACCAA CGAGGTGATC GCGTCGATCG CGGCAGCCAA CGGTGTGACG GTGCACCCGA ACGACCACGT GAACATGTCG CAGAGCTCCA ACGACACCTT CCCCACCGCG ACCCACATCG CGGCCACGGA AGCCGCTGTG CGCCATCTGA TCCCGGCGCT GGAGGTCCTG CACGCATCGC TGGCCGCCAA GGCCAAGCAG TGGCGCACCG TCGTCAAGTC CGGCCGCACG CACCTCATGG ACGCCGTGCC CGTCACGCTC GGGCAGGAGT TCGGCGGCTA TGCGCGCCAG ATCGAGGCAG GCATCGAACG CGTCAAGGCC ACGCTGCCAC GTCTCGGTGA ACTCGCGATC GGCGGCACCG CCGTCGGCAC CGGCCTCAAC GCGCCGGAAG GCTTCGGCGC CAAGGTCGTC GAGGTGCTCG TCAACGAGAC CGGCCTGGCC GAGTTGCGCA CCGCCGTCGA CTCGTTCGAG GCGCAGGCCG CGCGCGACGG GCTGGTCGAG GCCTCCGGTG CGCTGCGCAC CATCGCGGTG TCGCTCACCA AGATCGCCAA CGACATCCGC TGGATGGGCT CGGGCCCGCT GACCGGCCTG GCCGAGATCC AGCTGCCGGA CCTGCAGCCG GGCAGCTCGA TCATGCCGGG CAAGGTCAAC CCCGTCCTGC CCGAGGCCGT CACGCAGGTG GCCTGCCAGG TGGTCGGCAA CGACGCGGCG ATCGCGTTCG GCGGTGCGTC GGGTGCCTTC GAGCTCAACG TGTACATCCC GATGATGGCG CGCAACCTGC TCGAGTCGTT CACGTTGCTG TCCAACGTGT CCCGGCTGTT CGCCGAGCGC TGCATCGACG GCCTGGTGGC CAACGAGGAG CGCCTGCGGG AGCTCGCCGA GTCCTCGCCG TCGATCGTGA CGCCGCTGAA CTCGGCGATC GGTTACGAAG AGGCCGCCAA GGTCGCCAAG CAGGCTCTCG CGGAGAAGAA GACCATCCGC CAGACCGTGA TCGACCGTGG CCTCATCGGC GACAAGCTGA GCCTCGAGGA ACTCGACCGT CGTCTCGACG TGCTCGCCAT GGCGCGGGTC AAGGACGGCG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA