MyabA.00725.a
Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE)
| CENTER ID: | MyabA.00725.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00725.a.A1.GE30336 | Full length( MyabA.00725.a ) | 1 | 314 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00725.a.A1.PW30213 | Full length( MyabA.00725.a ) | 1 | 314 |
Structures
4DXL
DEPOSITED: 2/27/2012
DETERMINATION: XRay
CLONE: MyabA.00725.a.A1.GE30336
PROTEIN: MyabA.00725.a.A1.PW30213
4ED4
DEPOSITED: 3/26/2012
DETERMINATION: XRay
CLONE: MyabA.00725.a.A1.GE30336
PROTEIN: MyabA.00725.a.A1.PW30213
4EMD
DEPOSITED: 4/11/2012
DETERMINATION: XRay
CLONE: MyabA.00725.a.A1.GE30336
PROTEIN: MyabA.00725.a.A1.PW30213
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.1175 |
| RefSeq: | YP_001701881.1 |
| UniProt: | B1MKD5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETVSDWVP TGAVTVRAPG KVNLYLAVGD LRDDGYHELT TVFHAVSLAD DVTVRDADVL SIDVVGQGEG TVPTDERNLA WQAAELFADH VGRAPDVSIF INKDIPVAGG MAGGSADAAA VLVAMNELWH AGVPRRDLHH LAAQLGSDVP FALHGGTALG TGRGEQLATV LARNVFHWVF AFADGGLATP QVFKEIDRLR ENGDPPRLAE ADELLGALAA GDARRLAPLL GNELQAAAVS LNPELRRTLR AGESAGALAG IVSGSGPTCA FLCTSADDAV QVSAELAGAG VCRTVRVASG PVHGAQVIQG RSDG
NT Sequence
GTGTCCGAGA CCGTCTCCGA TTGGGTTCCT ACCGGCGCGG TGACTGTCCG GGCACCCGGC AAGGTCAATC TGTACCTGGC CGTAGGTGAC CTCCGTGACG ACGGCTATCA CGAGCTGACC ACGGTTTTTC ACGCCGTTTC GCTGGCCGAC GACGTGACCG TGCGGGACGC CGACGTGTTA TCGATCGATG TGGTCGGGCA GGGCGAGGGC ACGGTGCCCA CCGACGAACG CAATCTCGCC TGGCAGGCCG CCGAGCTTTT CGCCGATCAC GTGGGCCGCG CCCCCGACGT CTCGATCTTC ATTAACAAGG ACATCCCCGT CGCGGGCGGT ATGGCCGGCG GATCCGCCGA TGCCGCTGCG GTGTTGGTGG CGATGAACGA GCTCTGGCAT GCCGGTGTGC CGCGCCGGGA CCTGCACCAC CTGGCCGCTC AACTGGGCAG CGATGTCCCC TTTGCCTTAC ATGGCGGCAC CGCGCTGGGC ACTGGCCGGG GTGAGCAGCT GGCGACCGTA TTGGCGCGCA ATGTCTTTCA TTGGGTGTTC GCCTTCGCCG ACGGGGGACT GGCGACGCCT CAGGTCTTCA AGGAGATCGA CCGGTTGCGT GAGAACGGCG ATCCGCCGCG GCTCGCCGAG GCGGATGAGC TTTTGGGTGC GCTCGCCGCC GGGGACGCGC GCCGGCTGGC GCCGCTGCTC GGTAACGAAT TGCAGGCCGC GGCGGTCAGC CTGAACCCCG AATTGCGGCG CACGCTGCGG GCAGGGGAGT CGGCCGGCGC CCTGGCTGGA ATCGTCTCCG GATCGGGCCC CACCTGTGCG TTCTTGTGCA CCTCGGCCGA CGATGCGGTG CAGGTGAGTG CGGAGCTGGC GGGCGCGGGG GTGTGCCGCA CCGTGCGGGT GGCCAGCGGA CCCGTGCACG GCGCACAGGT CATCCAGGGC CGCAGTGACG GC
Details for MyabA.00725.a.A1.GE30336
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVSETVS DWVPTGAVTV RAPGKVNLYL AVGDLRDDGY HELTTVFHAV SLADDVTVRD ADVLSIDVVG QGEGTVPTDE RNLAWQAAEL FADHVGRAPD VSIFINKDIP VAGGMAGGSA DAAAVLVAMN ELWHAGVPRR DLHHLAAQLG SDVPFALHGG TALGTGRGEQ LATVLARNVF HWVFAFADGG LATPQVFKEI DRLRENGDPP RLAEADELLG ALAAGDARRL APLLGNELQA AAVSLNPELR RTLRAGESAG ALAGIVSGSG PTCAFLCTSA DDAVQVSAEL AGAGVCRTVR VASGPVHGAQ VIQGRS
Validated NT Sequence
cactgcggcc ctggatgacc tgtgcgccgt gcacgggtcc gctggccacc cgcacggtgc ggcacacccc cgcgcccgcc agctccgcac tcacctgcac cgcatcgtcg gccgaggtgc acaagaacgc acaggtgggg cccgatccgg agacgattcc agccagggcg ccggccgact cccctgcccg cagcgtgcgc cgcaattcgg ggttcaggct gaccgccgcg gcctgcaatt cgttaccgag cagcggcgcc agccggcgcg cgtccccggc ggcgagcgca cccaaaagct catccgcctc ggcgagccgc ggcggatcgc cgttctcacg caaccggtcg atctccttga agacctgagg cgtcgccagt cccccgtcgg cgaaggcgaa cacccaatga aagacattgc gcgccaatac ggtcgccagc tgctcacccc ggccagtgcc cagcgcggtg ccgccatgta aggcaaaggg gacatcgctg cccagttgag cggccaggtg gtgcaggtcc cggcgcggca caccggcatg ccagagctcg ttcatcgcca ccaacaccgc agcggcatcg gcggatccgc cggccatacc gcccgcgacg gggatgtcct tgttaatgaa gatcgagacg tcgggggcgc ggcccacgtg atcggcgaaa agctcggcgg cctgccaggc gagattgcgt tcgtcggtgg gcaccgtgcc ctcgccctgc ccgaccacat cgatcgataa cacgtcggcg tcccgcacgg tcacgtcgtc ggccagcgaa acggcgtgaa aaaccgtggt cagctcgtga tagccgtcgt cacggaggtc acctacggcc aggtacagat tgaccttgcc gggtgcccgg acagtcaccg cgccggtagg aacccaatcg gagacggtct cggacaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSETVSDW VPTGAVTVRA PGKVNLYLAV GDLRDDGYHE LTTVFHAVSL ADDVTVRDAD VLSIDVVGQG EGTVPTDERN LAWQAAELFA DHVGRAPDVS IFINKDIPVA GGMAGGSADA AAVLVAMNEL WHAGVPRRDL HHLAAQLGSD VPFALHGGTA LGTGRGEQLA TVLARNVFHW VFAFADGGLA TPQVFKEIDR LRENGDPPRL AEADELLGAL AAGDARRLAP LLGNELQAAA VSLNPELRRT LRAGESAGAL AGIVSGSGPT CAFLCTSADD AVQVSAELAG AGVCRTVRVA SGPVHGAQVI QGRSDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccgag accgtctccg attgggttcc taccggcgcg gtgactgtcc gggcacccgg caaggtcaat ctgtacctgg ccgtaggtga cctccgtgac gacggctatc acgagctgac cacggttttt cacgccgttt cgctggccga cgacgtgacc gtgcgggacg ccgacgtgtt atcgatcgat gtggtcgggc agggcgaggg cacggtgccc accgacgaac gcaatctcgc ctggcaggcc gccgagcttt tcgccgatca cgtgggccgc gcccccgacg tctcgatctt cattaacaag gacatccccg tcgcgggcgg tatggccggc ggatccgccg atgccgctgc ggtgttggtg gcgatgaacg agctctggca tgccggtgtg ccgcgccggg acctgcacca cctggccgct caactgggca gcgatgtccc ctttgcctta catggcggca ccgcgctggg cactggccgg ggtgagcagc tggcgaccgt attggcgcgc aatgtctttc attgggtgtt cgccttcgcc gacgggggac tggcgacgcc tcaggtcttc aaggagatcg accggttgcg tgagaacggc gatccgccgc ggctcgccga ggcggatgag cttttgggtg cgctcgccgc cggggacgcg cgccggctgg cgccgctgct cggtaacgaa ttgcaggccg cggcggtcag cctgaacccc gaattgcggc gcacgctgcg ggcaggggag tcggccggcg ccctggctgg aatcgtctcc ggatcgggcc ccacctgtgc gttcttgtgc acctcggccg acgatgcggt gcaggtgagt gcggagctgg cgggcgcggg ggtgtgccgc accgtgcggg tggccagcgg acccgtgcac ggcgcacagg tcatccaggg ccgcagtgac ggctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyabA.00725.a.A1.PW30213
| PURIFICATION DATe: | 12/13/2010 |
| CONCENTRATION: | 29.32mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVSETVS DWVPTGAVTV RAPGKVNLYL AVGDLRDDGY HELTTVFHAV SLADDVTVRD ADVLSIDVVG QGEGTVPTDE RNLAWQAAEL FADHVGRAPD VSIFINKDIP VAGGMAGGSA DAAAVLVAMN ELWHAGVPRR DLHHLAAQLG SDVPFALHGG TALGTGRGEQ LATVLARNVF HWVFAFADGG LATPQVFKEI DRLRENGDPP RLAEADELLG ALAAGDARRL APLLGNELQA AAVSLNPELR RTLRAGESAG ALAGIVSGSG PTCAFLCTSA DDAVQVSAEL AGAGVCRTVR VASGPVHGAQ VIQGRS
Validated NT Sequence
cactgcggcc ctggatgacc tgtgcgccgt gcacgggtcc gctggccacc cgcacggtgc ggcacacccc cgcgcccgcc agctccgcac tcacctgcac cgcatcgtcg gccgaggtgc acaagaacgc acaggtgggg cccgatccgg agacgattcc agccagggcg ccggccgact cccctgcccg cagcgtgcgc cgcaattcgg ggttcaggct gaccgccgcg gcctgcaatt cgttaccgag cagcggcgcc agccggcgcg cgtccccggc ggcgagcgca cccaaaagct catccgcctc ggcgagccgc ggcggatcgc cgttctcacg caaccggtcg atctccttga agacctgagg cgtcgccagt cccccgtcgg cgaaggcgaa cacccaatga aagacattgc gcgccaatac ggtcgccagc tgctcacccc ggccagtgcc cagcgcggtg ccgccatgta aggcaaaggg gacatcgctg cccagttgag cggccaggtg gtgcaggtcc cggcgcggca caccggcatg ccagagctcg ttcatcgcca ccaacaccgc agcggcatcg gcggatccgc cggccatacc gcccgcgacg gggatgtcct tgttaatgaa gatcgagacg tcgggggcgc ggcccacgtg atcggcgaaa agctcggcgg cctgccaggc gagattgcgt tcgtcggtgg gcaccgtgcc ctcgccctgc ccgaccacat cgatcgataa cacgtcggcg tcccgcacgg tcacgtcgtc ggccagcgaa acggcgtgaa aaaccgtggt cagctcgtga tagccgtcgt cacggaggtc acctacggcc aggtacagat tgaccttgcc gggtgcccgg acagtcaccg cgccggtagg aacccaatcg gagacggtct cggacaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSETVSDW VPTGAVTVRA PGKVNLYLAV GDLRDDGYHE LTTVFHAVSL ADDVTVRDAD VLSIDVVGQG EGTVPTDERN LAWQAAELFA DHVGRAPDVS IFINKDIPVA GGMAGGSADA AAVLVAMNEL WHAGVPRRDL HHLAAQLGSD VPFALHGGTA LGTGRGEQLA TVLARNVFHW VFAFADGGLA TPQVFKEIDR LRENGDPPRL AEADELLGAL AAGDARRLAP LLGNELQAAA VSLNPELRRT LRAGESAGAL AGIVSGSGPT CAFLCTSADD AVQVSAELAG AGVCRTVRVA SGPVHGAQVI QGRSDG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCCGAG ACCGTCTCCG ATTGGGTTCC TACCGGCGCG GTGACTGTCC GGGCACCCGG CAAGGTCAAT CTGTACCTGG CCGTAGGTGA CCTCCGTGAC GACGGCTATC ACGAGCTGAC CACGGTTTTT CACGCCGTTT CGCTGGCCGA CGACGTGACC GTGCGGGACG CCGACGTGTT ATCGATCGAT GTGGTCGGGC AGGGCGAGGG CACGGTGCCC ACCGACGAAC GCAATCTCGC CTGGCAGGCC GCCGAGCTTT TCGCCGATCA CGTGGGCCGC GCCCCCGACG TCTCGATCTT CATTAACAAG GACATCCCCG TCGCGGGCGG TATGGCCGGC GGATCCGCCG ATGCCGCTGC GGTGTTGGTG GCGATGAACG AGCTCTGGCA TGCCGGTGTG CCGCGCCGGG ACCTGCACCA CCTGGCCGCT CAACTGGGCA GCGATGTCCC CTTTGCCTTA CATGGCGGCA CCGCGCTGGG CACTGGCCGG GGTGAGCAGC TGGCGACCGT ATTGGCGCGC AATGTCTTTC ATTGGGTGTT CGCCTTCGCC GACGGGGGAC TGGCGACGCC TCAGGTCTTC AAGGAGATCG ACCGGTTGCG TGAGAACGGC GATCCGCCGC GGCTCGCCGA GGCGGATGAG CTTTTGGGTG CGCTCGCCGC CGGGGACGCG CGCCGGCTGG CGCCGCTGCT CGGTAACGAA TTGCAGGCCG CGGCGGTCAG CCTGAACCCC GAATTGCGGC GCACGCTGCG GGCAGGGGAG TCGGCCGGCG CCCTGGCTGG AATCGTCTCC GGATCGGGCC CCACCTGTGC GTTCTTGTGC ACCTCGGCCG ACGATGCGGT GCAGGTGAGT GCGGAGCTGG CGGGCGCGGG GGTGTGCCGC ACCGTGCGGG TGGCCAGCGG ACCCGTGCAC GGCGCACAGG TCATCCAGGG CCGCAGTGAC GGCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA