MyulA.00725.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE)

CENTER ID:	MyulA.00725.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00725.a.B1.GE40304	Full length( MyulA.00725.a )		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130885
PATRIC ID:	fig\|362242.7.peg.5372
RefSeq:	YP_908059.1
UniProt:	A0PW69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTGSATVRV PGKVNLYLAV GDRRDDGYHE LTTVFHAVSL VDEVTVRNAD LLSLEVVGEG ADKLPTDKRN LAWQAAELMA EHVGRAPDVS IFIDKSIPVA GGMAGGSADA AAVLVAMNSL WELNLPRRDL RMLAAQLGSD VPFALHGGTA LGTGRGEELA TVLSRNTFHW VLAFARSGLS TPAVFTELDR LRDVGSPPRL AEPGPVLAAL AAGDPEQLAP LLGNEMQAAA VSLDPALRRA LRAGVEAGAL AGIVSGSGPT CAFLCRTAES ALDVSAQLSG AGVCRTVRIA TGPVPGARVV PTPGTPE

NT Sequence

GTGCCTACCG GGTCGGCGAC CGTAAGGGTC CCCGGCAAGG TCAACCTGTA CCTGGCGGTC GGCGATCGCC GCGATGACGG CTATCACGAG TTGACCACGG TGTTTCACGC GGTCTCCCTG GTCGACGAGG TGACGGTGCG CAACGCCGAC CTGCTGTCGC TGGAGGTCGT CGGCGAGGGC GCCGACAAAC TGCCCACCGA CAAACGCAAC CTCGCCTGGC AGGCGGCCGA GCTGATGGCC GAACACGTCG GCCGCGCACC GGACGTGTCG ATCTTCATCG ACAAGTCGAT TCCGGTGGCC GGCGGCATGG CCGGGGGCAG CGCCGACGCG GCCGCGGTAC TGGTGGCGAT GAATTCGCTG TGGGAGCTCA ACCTGCCCCG GCGTGACCTG CGCATGCTGG CCGCGCAGCT GGGCAGTGAT GTGCCCTTTG CGCTGCACGG CGGTACCGCG CTGGGCACGG GCCGCGGCGA GGAACTGGCG ACGGTCTTGT CCCGGAATAC CTTCCACTGG GTGCTGGCGT TCGCACGCAG TGGCCTGTCC ACCCCGGCGG TGTTCACCGA GCTGGACCGG CTCAGGGACG TGGGGAGTCC ACCTCGGCTC GCCGAGCCCG GGCCGGTGCT GGCGGCTCTG GCGGCCGGCG ATCCCGAGCA GCTGGCGCCC CTGCTGGGTA ACGAGATGCA GGCCGCCGCG GTGAGCCTGG ACCCGGCGCT GCGCCGGGCG CTGCGTGCCG GGGTGGAGGC CGGCGCCCTC GCCGGCATCG TCTCGGGCTC CGGTCCGACG TGTGCCTTCC TGTGCCGCAC GGCCGAGTCC GCGCTGGATG TCAGCGCGCA GCTGTCGGGA GCGGGCGTCT GTCGTACCGT TCGAATCGCC ACCGGACCGG TGCCGGGAGC CCGGGTGGTG CCGACGCCGG GAACCCCGGA G

Details for MyulA.00725.a.B1.GE40304

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/16/2016
EXPECTED MW:	33kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP TGSATVRVPG KVNLYLAVGD RRDDGYHELT TVFHAVSLVD EVTVRNADLL SLEVVGEGAD KLPTDKRNLA WQAAELMAEH VGRAPDVSIF IDKSIPVAGG MAGGSADAAA VLVAMNSLWE LNLPRRDLRM LAAQLGSDVP FALHGGTALG TGRGEELATV LSRNTFHWVL AFARSGLSTP AVFTELDRLR DVGSPPRLAE PGPVLAALAA GDPEQLAPLL GNEMQAAAVS LDPALRRALR AGVEAGALAG IVSGSGPTCA FLCRTAESAL DVSAQLSGAG VCRTVRIATG PVPGARVVPT PGTPE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgccta ccgggtcggc gaccgtaagg gtccccggca aggtcaacct gtacctggcg gtcggcgatc gccgcgatga cggctatcac gagttgacca cggtgtttca cgcggtctcc ctggtcgacg aggtgacggt gcgcaacgcc gacctgctgt cgctggaggt cgtcggcgag ggcgccgaca aactgcccac cgacaaacgc aacctcgcct ggcaggcggc cgagctgatg gccgaacacg tcggccgcgc accggacgtg tcgatcttca tcgacaagtc gattccggtg gccggcggca tggccggggg cagcgccgac gcggccgcgg tactggtggc gatgaattcg ctgtgggagc tcaacctgcc ccggcgtgac ctgcgcatgc tggccgcgca gctgggcagt gatgtgccct ttgcgctgca cggcggtacc gcgctgggca cgggccgcgg cgaggaactg gcgacggtct tgtcccggaa taccttccac tgggtgctgg cgttcgcacg cagtggcctg tccaccccgg cggtgttcac cgagctggac cggctcaggg acgtggggag tccacctcgg ctcgccgagc ccgggccggt gctggcggct ctggcggccg gcgatcccga gcagctggcg cccctgctgg gtaacgagat gcaggccgcc gcggtgagcc tggacccggc gctgcgccgg gcgctgcgtg ccggggtgga ggccggcgcc ctcgccggca tcgtctcggg ctccggtccg acgtgtgcct tcctgtgccg cacggccgag tccgcgctgg atgtcagcgc gcagctgtcg ggagcgggcg tctgtcgtac cgttcgaatc gccaccggac cggtgccggg agcccgggtg gtgccgacgc cgggaacccc ggagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat