SSGCID: Target Page

MysmA.00725.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE)

CENTER ID:	MysmA.00725.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00725.a.B1.GE40129	Full length( MysmA.00725.a )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00725.a.B1.PS38006	Full length( MysmA.00725.a )		1	322

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5297
RefSeq:	YP_889675.1
UniProt:	A0R3D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVPASDGST ASEWVTTGSV TVRVPGKVNL YLSVGDLRDD GYHDLTTVFH AVSLLDEVTV RNADVLSLRM IGEGAESLPA DERNLAWRAA ELMAEHVGRA PDVEITIEKS IPVAGGMAGG SADAAGVLVA MNTLWELGVP RRDLHALAAQ LGSDVPFALH GGTALGSGRG EDLATVLARN TFHWVLAFAH RGLSTPKVFG ELDRLRADTT RGLPRTDEPE PVLAALASGD PAELAALLGN DLQPAALSLY PDLRRTLRAG VEAGALAGIV SGSGPTCAFL CASAPAALDV GTQLSGAGVC RTVRVASGPV HGAHVVPAPS AS

NT Sequence

GTGGCCGTGC CCGCATCCGA TGGCAGTACC GCTTCCGAAT GGGTGACCAC CGGCTCGGTC ACCGTGCGCG TCCCCGGCAA GGTGAACCTG TACCTGTCCG TGGGCGATCT GCGCGACGAC GGGTACCACG ATCTGACGAC GGTGTTCCAC GCGGTCTCGC TGCTCGACGA GGTGACGGTC CGCAACGCCG ACGTGCTGTC GCTGCGGATG ATCGGGGAGG GCGCCGAGTC GCTGCCGGCC GACGAGCGCA ATCTGGCGTG GCGCGCCGCC GAGTTGATGG CCGAGCACGT CGGCCGCGCC CCCGATGTCG AGATCACGAT CGAGAAGTCC ATCCCGGTGG CCGGCGGGAT GGCCGGTGGC TCGGCGGATG CCGCGGGCGT GCTGGTCGCG ATGAACACGC TGTGGGAGCT CGGCGTGCCG CGGCGGGATC TGCACGCGCT GGCCGCACAG CTGGGCAGTG ATGTGCCGTT CGCGCTGCAC GGCGGGACCG CGCTGGGGTC CGGGCGCGGC GAGGACCTCG CCACGGTACT CGCGCGCAAC ACGTTCCACT GGGTGCTGGC GTTCGCGCAC CGTGGCTTGT CGACGCCCAA GGTCTTCGGG GAACTGGACC GGTTGCGCGC CGACACCACC CGTGGTCTGC CGCGCACCGA CGAGCCCGAG CCCGTGCTGG CCGCGCTGGC CTCGGGCGAT CCGGCCGAGC TGGCGGCCCT GCTGGGCAAC GATCTGCAGC CCGCGGCGCT GAGCCTGTAT CCCGACCTGC GTCGCACGCT GCGTGCCGGT GTCGAGGCGG GTGCGCTCGC GGGCATCGTG TCGGGGTCCG GTCCGACGTG TGCGTTCCTG TGTGCGTCGG CCCCGGCCGC ACTGGATGTC GGCACGCAGC TGTCGGGGGC CGGGGTGTGC CGCACCGTGC GCGTGGCGAG CGGGCCTGTA CACGGCGCGC ACGTCGTTCC CGCGCCGTCG GCATCG

Details for MysmA.00725.a.B1.GE40129

HARVESTED ON:	4/6/2016
SEQUENCED ON:	4/13/2016
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMV AVPASDGSTA SEWVTTGSVT VRVPGKVNLY LSVGDLRDDG YHDLTTVFHA VSLLDEVTVR NADVLSLRMI GEGAESLPAD ERNLAWRAAE LMAEHVGRAP DVEITIEKSI PVAGGMAGGS ADAAGVLVAM NTLWELGVPR RDLHALAAQL GSDVPFALHG GTALGSGRGE DLATVLARNT FHWVLAFAHR GLSTPKVFGE LDRLRADTTR GLPRTDEPEP VLAALASGDP AELAALLGND LQPAALSLYP DLRRTLRAGV EAGALAGIVS GSGPTCAFLC ASAPAALDVG TQLSGAGVCR TVRVASGPVH GA

Validated NT Sequence

gtgcgcgccg tgtacaggcc cgctcgccac gcgcacggtg cggcacaccc cggcccccga cagctgcgtg ccgacatcca gtgcggccgg ggccgacgca cacaggaacg cacacgtcgg accggacccc gacacgatgc ccgcgagcgc acccgcctcg acaccggcac gcagcgtgcg acgcaggtcg ggatacaggc tcagcgccgc gggctgcaga tcgttgccca gcagggccgc cagctcggcc ggatcgcccg aggccagcgc ggccagcacg ggctcgggct cgtcggtgcg cggcagacca cgggtggtgt cggcgcgcaa ccggtccagt tccccgaaga ccttgggcgt cgacaagcca cggtgcgcga acgccagcac ccagtggaac gtgttgcgcg cgagtaccgt ggcgaggtcc tcgccgcgcc cggaccccag cgcggtcccg ccgtgcagcg cgaacggcac atcactgccc agctgtgcgg ccagcgcgtg cagatcccgc cgcggcacgc cgagctccca cagcgtgttc atcgcgacca gcacgcccgc ggcatccgcc gagccaccgg ccatcccgcc ggccaccggg atggacttct cgatcgtgat ctcgacatcg ggggcgcggc cgacgtgctc ggccatcaac tcggcggcgc gccacgccag attgcgctcg tcggccggca gcgactcggc gccctccccg atcatccgca gcgacagcac gtcggcgttg cggaccgtca cctcgtcgag cagcgagacc gcgtggaaca ccgtcgtcag atcgtggtac ccgtcgtcgc gcagatcgcc cacggacagg tacaggttca ccttgccggg gacgcgcacg gtgaccgagc cggtggtcac ccattcggaa gcggtactgc catcggatgc gggcacggcc accatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMA VPASDGSTAS EWVTTGSVTV RVPGKVNLYL SVGDLRDDGY HDLTTVFHAV SLLDEVTVRN ADVLSLRMIG EGAESLPADE RNLAWRAAEL MAEHVGRAPD VEITIEKSIP VAGGMAGGSA DAAGVLVAMN TLWELGVPRR DLHALAAQLG SDVPFALHGG TALGSGRGED LATVLARNTF HWVLAFAHRG LSTPKVFGEL DRLRADTTRG LPRTDEPEPV LAALASGDPA ELAALLGNDL QPAALSLYPD LRRTLRAGVE AGALAGIVSG SGPTCAFLCA SAPAALDVGT QLSGAGVCRT VRVASGPVHG AHVVPAPSAS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggccg tgcccgcatc cgatggcagt accgcttccg aatgggtgac caccggctcg gtcaccgtgc gcgtccccgg caaggtgaac ctgtacctgt ccgtgggcga tctgcgcgac gacgggtacc acgatctgac gacggtgttc cacgcggtct cgctgctcga cgaggtgacg gtccgcaacg ccgacgtgct gtcgctgcgg atgatcgggg agggcgccga gtcgctgccg gccgacgagc gcaatctggc gtggcgcgcc gccgagttga tggccgagca cgtcggccgc gcccccgatg tcgagatcac gatcgagaag tccatcccgg tggccggcgg gatggccggt ggctcggcgg atgccgcggg cgtgctggtc gcgatgaaca cgctgtggga gctcggcgtg ccgcggcggg atctgcacgc gctggccgca cagctgggca gtgatgtgcc gttcgcgctg cacggcggga ccgcgctggg gtccgggcgc ggcgaggacc tcgccacggt actcgcgcgc aacacgttcc actgggtgct ggcgttcgcg caccgtggct tgtcgacgcc caaggtcttc ggggaactgg accggttgcg cgccgacacc acccgtggtc tgccgcgcac cgacgagccc gagcccgtgc tggccgcgct ggcctcgggc gatccggccg agctggcggc cctgctgggc aacgatctgc agcccgcggc gctgagcctg tatcccgacc tgcgtcgcac gctgcgtgcc ggtgtcgagg cgggtgcgct cgcgggcatc gtgtcggggt ccggtccgac gtgtgcgttc ctgtgtgcgt cggccccggc cgcactggat gtcggcacgc agctgtcggg ggccggggtg tgccgcaccg tgcgcgtggc gagcgggcct gtacacggcg cgcacgtcgt tcccgcgccg tcggcatcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for MysmA.00725.a.B1.PS38006

PURIFICATION DATe:	8/4/2016
CONCENTRATION:	13.62mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGCCG TGCCCGCATC CGATGGCAGT ACCGCTTCCG AATGGGTGAC CACCGGCTCG GTCACCGTGC GCGTCCCCGG CAAGGTGAAC CTGTACCTGT CCGTGGGCGA TCTGCGCGAC GACGGGTACC ACGATCTGAC GACGGTGTTC CACGCGGTCT CGCTGCTCGA CGAGGTGACG GTCCGCAACG CCGACGTGCT GTCGCTGCGG ATGATCGGGG AGGGCGCCGA GTCGCTGCCG GCCGACGAGC GCAATCTGGC GTGGCGCGCC GCCGAGTTGA TGGCCGAGCA CGTCGGCCGC GCCCCCGATG TCGAGATCAC GATCGAGAAG TCCATCCCGG TGGCCGGCGG GATGGCCGGT GGCTCGGCGG ATGCCGCGGG CGTGCTGGTC GCGATGAACA CGCTGTGGGA GCTCGGCGTG CCGCGGCGGG ATCTGCACGC GCTGGCCGCA CAGCTGGGCA GTGATGTGCC GTTCGCGCTG CACGGCGGGA CCGCGCTGGG GTCCGGGCGC GGCGAGGACC TCGCCACGGT ACTCGCGCGC AACACGTTCC ACTGGGTGCT GGCGTTCGCG CACCGTGGCT TGTCGACGCC CAAGGTCTTC GGGGAACTGG ACCGGTTGCG CGCCGACACC ACCCGTGGTC TGCCGCGCAC CGACGAGCCC GAGCCCGTGC TGGCCGCGCT GGCCTCGGGC GATCCGGCCG AGCTGGCGGC CCTGCTGGGC AACGATCTGC AGCCCGCGGC GCTGAGCCTG TATCCCGACC TGCGTCGCAC GCTGCGTGCC GGTGTCGAGG CGGGTGCGCT CGCGGGCATC GTGTCGGGGT CCGGTCCGAC GTGTGCGTTC CTGTGTGCGT CGGCCCCGGC CGCACTGGAT GTCGGCACGC AGCTGTCGGG GGCCGGGGTG TGCCGCACCG TGCGCGTGGC GAGCGGGCCT GTACACGGCG CGCACGTCGT TCCCGCGCCG TCGGCATCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT