MymaA.00725.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE)

CENTER ID: MymaA.00725.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00725.a.A1.GE30062 Full length( MymaA.00725.a ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00725.a.A1.PW33580 Full length( MymaA.00725.a ) 1 307 unavailable
MymaA.00725.a.A1.PW33580a Full length( MymaA.00725.a ) 1 307 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4810
RefSeq: YP_001852738.1
UniProt: B2HDJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTGSATVRV PGKVNLYLAV GDRRDDGYHE LTTVFHAVSL VDEVTVRNAD LLSLEVVGEG ADRLPTDKRN LAWQAAELMA EHVGRAPDVS IFIDKSIPVA GGMAGGSADA AAVLVAMNSL WELNLPRRDL RMLAARLGSD VPFALHGGTA LGTGRGEELA TVLSRNTFHW VLAFARSGLS TPAVFTELDR LRDVGSPPRL AEPGPVLAAL AAGDPEQLAP LLGNEMQAAA VSLDPALRRA LRAGVEAGAL AGIVSGSGPT CAFLCRTAES ALDVSAQLSG AGVCRTVRIA TGPVPGARVV PTPGIAE
NT Sequence
GTGCCTACCG GGTCGGCGAC CGTAAGGGTC CCCGGCAAGG TCAACCTGTA CCTGGCGGTC GGCGATCGCC GCGATGACGG CTATCACGAG CTGACCACGG TGTTTCACGC GGTCTCCCTG GTCGACGAGG TGACGGTGCG CAACGCCGAC CTGCTGTCGC TGGAGGTCGT CGGCGAGGGC GCCGACAGAC TGCCCACCGA CAAACGCAAC CTTGCCTGGC AGGCGGCCGA GCTGATGGCC GAACACGTCG GCCGCGCACC GGACGTGTCG ATCTTCATCG ACAAGTCGAT TCCGGTGGCC GGCGGCATGG CCGGGGGCAG CGCCGACGCG GCCGCGGTAC TGGTGGCGAT GAATTCGCTG TGGGAGCTCA ACTTGCCCCG GCGCGACCTG CGCATGCTGG CCGCGCGGCT GGGCAGTGAT GTGCCCTTTG CGCTGCACGG CGGTACCGCG CTGGGCACGG GCCGCGGCGA GGAACTGGCG ACGGTCTTGT CCCGGAATAC CTTCCACTGG GTGCTGGCGT TCGCACGCAG CGGCCTGTCC ACCCCGGCGG TGTTCACCGA GCTCGACCGG CTCAGGGACG TGGGGAGTCC ACCTCGGCTC GCCGAGCCCG GGCCGGTGCT GGCGGCTCTG GCGGCCGGCG ATCCCGAGCA GCTGGCGCCC CTGCTGGGTA ACGAGATGCA GGCCGCCGCG GTGAGTCTGG ACCCGGCGCT GCGCCGGGCG CTGCGTGCCG GGGTGGAGGC CGGCGCCCTC GCCGGCATCG TCTCGGGCTC CGGTCCGACG TGTGCCTTCC TGTGCCGCAC GGCCGAGTCC GCGCTGGATG TCAGCGCGCA GCTGTCGGGA GCGGGCGTCT GTCGTACCGT TCGAATCGCC ACCGGACCGG TGCCGGGAGC CCGGGTGGTG CCGACGCCGG GAATCGCGGA G
Details for MymaA.00725.a.A1.GE30062
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 89
Validated AA Sequence
MVPTGSATVR VPGKVNLYLA VGDRRDDGYH ELTTVFHAVS LVDEVTVRNA DLLSLEVVGE GADRLPTDKR NLAWQAAELM AEHVGRAPDV SIFIDKSIPV AGGMAGGSAD AAAVLVAMNS LWELNLPRRD LRMLAARLGS DVPFALHGGT ALGTGRGEEL ATVLSRNTFH WVLAFARSGL STPAVFTELD RLRDVGSPPR LAEPGPVLAA LAAGDPEQLA PLLGNEMQAA AVSLDPALRR ALRAGVEAGA LAGIVSGSGP TCXFLCRTXX SXLDVSAXLS GAGVCXTXRX AXGX
Validated NT Sequence
cngntccggn ggcgantcga anggtangac agacgccngc tcccgacagn tgcgcgctga catccagcgn ggantcgncc gtgcggcaca ggaagncaca cgtnggaccg gagcccgaga cgatgccggc gagggcgccg gcctccaccc cggcacgcag cgcccggcgc agcgccgggt ccagactcac cgcggcggcc tgcatctcgt tacccagcag gggcgccagc tgctcgggat cgccggccgc cagagccgcc agcaccggcc cgggctcggc gagccgaggt ggactcccca cgtccctgag ccggtcgagc tcggtgaaca ccgccggggt ggacaggccg ctgcgtgcga acgccagcac ccagtggaag gtattccggg acaagaccgt cgccagttcc tcgccgcggc ccgtgcccag cgcggtaccg ccgtgcagcg caaagggcac atcactgccc agccgcgcgg ccagcatgcg caggtcgcgc cggggcaagt tgagctccca cagcgaattc atcgccacca gtaccgcggc cgcgtcggcg ctgcccccgg ccatgccgcc ggccaccgga atcgacttgt cgatgaagat cgacacgtcc ggtgcgcggc cgacgtgttc ggccatcagc tcggccgcct gccaggcaag gttgcgtttg tcggtgggca gtctgtcggc gccctcgccg acgacctcca gcgacagcag gtcggcgttg cgcaccgtca cctcgtcgac cagggagacc gcgtgaaaca ccgtggtcag ctcgtgatag ccgtcatcgc ggcgatcgcc gaccgccagg tacaggttga ccttgccggg gacccttacg gtcgccgacc cggtaggcac catcgaacca ggaccctggg tctgagcttc caggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPTGSATV RVPGKVNLYL AVGDRRDDGY HELTTVFHAV SLVDEVTVRN ADLLSLEVVG EGADRLPTDK RNLAWQAAEL MAEHVGRAPD VSIFIDKSIP VAGGMAGGSA DAAAVLVAMN SLWELNLPRR DLRMLAARLG SDVPFALHGG TALGTGRGEE LATVLSRNTF HWVLAFARSG LSTPAVFTEL DRLRDVGSPP RLAEPGPVLA ALAAGDPEQL APLLGNEMQA AAVSLDPALR RALRAGVEAG ALAGIVSGSG PTCAFLCRTA ESALDVSAQL SGAGVCRTVR IATGPVPGAR VVPTPGIA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcctacc gggtcggcga ccgtaagggt ccccggcaag gtcaacctgt acctggcggt cggcgatcgc cgcgatgacg gctatcacga gctgaccacg gtgtttcacg cggtctccct ggtcgacgag gtgacggtgc gcaacgccga cctgctgtcg ctggaggtcg tcggcgaggg cgccgacaga ctgcccaccg acaaacgcaa ccttgcctgg caggcggccg agctgatggc cgaacacgtc ggccgcgcac cggacgtgtc gatcttcatc gacaagtcga ttccggtggc cggcggcatg gccgggggca gcgccgacgc ggccgcggta ctggtggcga tgaattcgct gtgggagctc aacttgcccc ggcgcgacct gcgcatgctg gccgcgcggc tgggcagtga tgtgcccttt gcgctgcacg gcggtaccgc gctgggcacg ggccgcggcg aggaactggc gacggtcttg tcccggaata ccttccactg ggtgctggcg ttcgcacgca gcggcctgtc caccccggcg gtgttcaccg agctcgaccg gctcagggac gtggggagtc cacctcggct cgccgagccc gggccggtgc tggcggctct ggcggccggc gatcccgagc agctggcgcc cctgctgggt aacgagatgc aggccgccgc ggtgagtctg gacccggcgc tgcgccgggc gctgcgtgcc ggggtggagg ccggcgccct cgccggcatc gtctcgggct ccggtccgac gtgtgccttc ctgtgccgca cggccgagtc cgcgctggat gtcagcgcgc agctgtcggg agcgggcgtc tgtcgtaccg ttcgaatcgc caccggaccg gtgccgggag cccgggtggt gccgacgccg ggaatcgcga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MymaA.00725.a.A1.PW33580
PURIFICATION DATe: 6/22/2011
CONCENTRATION: 47.49mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 96
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MVPTGSATVR VPGKVNLYLA VGDRRDDGYH ELTTVFHAVS LVDEVTVRNA DLLSLEVVGE GADRLPTDKR NLAWQAAELM AEHVGRAPDV SIFIDKSIPV AGGMAGGSAD AAAVLVAMNS LWELNLPRRD LRMLAARLGS DVPFALHGGT ALGTGRGEEL ATVLSRNTFH WVLAFARSGL STPAVFTELD RLRDVGSPPR LAEPGPVLAA LAAGDPEQLA PLLGNEMQAA AVSLDPALRR ALRAGVEAGA LAGIVSGSGP TCXFLCRTXX SXLDVSAXLS GAGVCXTXRX AXGX
Validated NT Sequence
cngntccggn ggcgantcga anggtangac agacgccngc tcccgacagn tgcgcgctga catccagcgn ggantcgncc gtgcggcaca ggaagncaca cgtnggaccg gagcccgaga cgatgccggc gagggcgccg gcctccaccc cggcacgcag cgcccggcgc agcgccgggt ccagactcac cgcggcggcc tgcatctcgt tacccagcag gggcgccagc tgctcgggat cgccggccgc cagagccgcc agcaccggcc cgggctcggc gagccgaggt ggactcccca cgtccctgag ccggtcgagc tcggtgaaca ccgccggggt ggacaggccg ctgcgtgcga acgccagcac ccagtggaag gtattccggg acaagaccgt cgccagttcc tcgccgcggc ccgtgcccag cgcggtaccg ccgtgcagcg caaagggcac atcactgccc agccgcgcgg ccagcatgcg caggtcgcgc cggggcaagt tgagctccca cagcgaattc atcgccacca gtaccgcggc cgcgtcggcg ctgcccccgg ccatgccgcc ggccaccgga atcgacttgt cgatgaagat cgacacgtcc ggtgcgcggc cgacgtgttc ggccatcagc tcggccgcct gccaggcaag gttgcgtttg tcggtgggca gtctgtcggc gccctcgccg acgacctcca gcgacagcag gtcggcgttg cgcaccgtca cctcgtcgac cagggagacc gcgtgaaaca ccgtggtcag ctcgtgatag ccgtcatcgc ggcgatcgcc gaccgccagg tacaggttga ccttgccggg gacccttacg gtcgccgacc cggtaggcac catcgaacca ggaccctggg tctgagcttc caggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPTGSATV RVPGKVNLYL AVGDRRDDGY HELTTVFHAV SLVDEVTVRN ADLLSLEVVG EGADRLPTDK RNLAWQAAEL MAEHVGRAPD VSIFIDKSIP VAGGMAGGSA DAAAVLVAMN SLWELNLPRR DLRMLAARLG SDVPFALHGG TALGTGRGEE LATVLSRNTF HWVLAFARSG LSTPAVFTEL DRLRDVGSPP RLAEPGPVLA ALAAGDPEQL APLLGNEMQA AAVSLDPALR RALRAGVEAG ALAGIVSGSG PTCAFLCRTA ESALDVSAQL SGAGVCRTVR IATGPVPGAR VVPTPGIA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCTACC GGGTCGGCGA CCGTAAGGGT CCCCGGCAAG GTCAACCTGT ACCTGGCGGT CGGCGATCGC CGCGATGACG GCTATCACGA GCTGACCACG GTGTTTCACG CGGTCTCCCT GGTCGACGAG GTGACGGTGC GCAACGCCGA CCTGCTGTCG CTGGAGGTCG TCGGCGAGGG CGCCGACAGA CTGCCCACCG ACAAACGCAA CCTTGCCTGG CAGGCGGCCG AGCTGATGGC CGAACACGTC GGCCGCGCAC CGGACGTGTC GATCTTCATC GACAAGTCGA TTCCGGTGGC CGGCGGCATG GCCGGGGGCA GCGCCGACGC GGCCGCGGTA CTGGTGGCGA TGAATTCGCT GTGGGAGCTC AACTTGCCCC GGCGCGACCT GCGCATGCTG GCCGCGCGGC TGGGCAGTGA TGTGCCCTTT GCGCTGCACG GCGGTACCGC GCTGGGCACG GGCCGCGGCG AGGAACTGGC GACGGTCTTG TCCCGGAATA CCTTCCACTG GGTGCTGGCG TTCGCACGCA GCGGCCTGTC CACCCCGGCG GTGTTCACCG AGCTCGACCG GCTCAGGGAC GTGGGGAGTC CACCTCGGCT CGCCGAGCCC GGGCCGGTGC TGGCGGCTCT GGCGGCCGGC GATCCCGAGC AGCTGGCGCC CCTGCTGGGT AACGAGATGC AGGCCGCCGC GGTGAGTCTG GACCCGGCGC TGCGCCGGGC GCTGCGTGCC GGGGTGGAGG CCGGCGCCCT CGCCGGCATC GTCTCGGGCT CCGGTCCGAC GTGTGCCTTC CTGTGCCGCA CGGCCGAGTC CGCGCTGGAT GTCAGCGCGC AGCTGTCGGG AGCGGGCGTC TGTCGTACCG TTCGAATCGC CACCGGACCG GTGCCGGGAG CCCGGGTGGT GCCGACGCCG GGAATCGCGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MymaA.00725.a.A1.PW33580a
PURIFICATION DATe: 6/22/2011
CONCENTRATION: 47.49mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 96
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MVPTGSATVR VPGKVNLYLA VGDRRDDGYH ELTTVFHAVS LVDEVTVRNA DLLSLEVVGE GADRLPTDKR NLAWQAAELM AEHVGRAPDV SIFIDKSIPV AGGMAGGSAD AAAVLVAMNS LWELNLPRRD LRMLAARLGS DVPFALHGGT ALGTGRGEEL ATVLSRNTFH WVLAFARSGL STPAVFTELD RLRDVGSPPR LAEPGPVLAA LAAGDPEQLA PLLGNEMQAA AVSLDPALRR ALRAGVEAGA LAGIVSGSGP TCXFLCRTXX SXLDVSAXLS GAGVCXTXRX AXGX
Validated NT Sequence
cngntccggn ggcgantcga anggtangac agacgccngc tcccgacagn tgcgcgctga catccagcgn ggantcgncc gtgcggcaca ggaagncaca cgtnggaccg gagcccgaga cgatgccggc gagggcgccg gcctccaccc cggcacgcag cgcccggcgc agcgccgggt ccagactcac cgcggcggcc tgcatctcgt tacccagcag gggcgccagc tgctcgggat cgccggccgc cagagccgcc agcaccggcc cgggctcggc gagccgaggt ggactcccca cgtccctgag ccggtcgagc tcggtgaaca ccgccggggt ggacaggccg ctgcgtgcga acgccagcac ccagtggaag gtattccggg acaagaccgt cgccagttcc tcgccgcggc ccgtgcccag cgcggtaccg ccgtgcagcg caaagggcac atcactgccc agccgcgcgg ccagcatgcg caggtcgcgc cggggcaagt tgagctccca cagcgaattc atcgccacca gtaccgcggc cgcgtcggcg ctgcccccgg ccatgccgcc ggccaccgga atcgacttgt cgatgaagat cgacacgtcc ggtgcgcggc cgacgtgttc ggccatcagc tcggccgcct gccaggcaag gttgcgtttg tcggtgggca gtctgtcggc gccctcgccg acgacctcca gcgacagcag gtcggcgttg cgcaccgtca cctcgtcgac cagggagacc gcgtgaaaca ccgtggtcag ctcgtgatag ccgtcatcgc ggcgatcgcc gaccgccagg tacaggttga ccttgccggg gacccttacg gtcgccgacc cggtaggcac catcgaacca ggaccctggg tctgagcttc caggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPTGSATV RVPGKVNLYL AVGDRRDDGY HELTTVFHAV SLVDEVTVRN ADLLSLEVVG EGADRLPTDK RNLAWQAAEL MAEHVGRAPD VSIFIDKSIP VAGGMAGGSA DAAAVLVAMN SLWELNLPRR DLRMLAARLG SDVPFALHGG TALGTGRGEE LATVLSRNTF HWVLAFARSG LSTPAVFTEL DRLRDVGSPP RLAEPGPVLA ALAAGDPEQL APLLGNEMQA AAVSLDPALR RALRAGVEAG ALAGIVSGSG PTCAFLCRTA ESALDVSAQL SGAGVCRTVR IATGPVPGAR VVPTPGIA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCTACC GGGTCGGCGA CCGTAAGGGT CCCCGGCAAG GTCAACCTGT ACCTGGCGGT CGGCGATCGC CGCGATGACG GCTATCACGA GCTGACCACG GTGTTTCACG CGGTCTCCCT GGTCGACGAG GTGACGGTGC GCAACGCCGA CCTGCTGTCG CTGGAGGTCG TCGGCGAGGG CGCCGACAGA CTGCCCACCG ACAAACGCAA CCTTGCCTGG CAGGCGGCCG AGCTGATGGC CGAACACGTC GGCCGCGCAC CGGACGTGTC GATCTTCATC GACAAGTCGA TTCCGGTGGC CGGCGGCATG GCCGGGGGCA GCGCCGACGC GGCCGCGGTA CTGGTGGCGA TGAATTCGCT GTGGGAGCTC AACTTGCCCC GGCGCGACCT GCGCATGCTG GCCGCGCGGC TGGGCAGTGA TGTGCCCTTT GCGCTGCACG GCGGTACCGC GCTGGGCACG GGCCGCGGCG AGGAACTGGC GACGGTCTTG TCCCGGAATA CCTTCCACTG GGTGCTGGCG TTCGCACGCA GCGGCCTGTC CACCCCGGCG GTGTTCACCG AGCTCGACCG GCTCAGGGAC GTGGGGAGTC CACCTCGGCT CGCCGAGCCC GGGCCGGTGC TGGCGGCTCT GGCGGCCGGC GATCCCGAGC AGCTGGCGCC CCTGCTGGGT AACGAGATGC AGGCCGCCGC GGTGAGTCTG GACCCGGCGC TGCGCCGGGC GCTGCGTGCC GGGGTGGAGG CCGGCGCCCT CGCCGGCATC GTCTCGGGCT CCGGTCCGAC GTGTGCCTTC CTGTGCCGCA CGGCCGAGTC CGCGCTGGAT GTCAGCGCGC AGCTGTCGGG AGCGGGCGTC TGTCGTACCG TTCGAATCGC CACCGGACCG GTGCCGGGAG CCCGGGTGGT GCCGACGCCG GGAATCGCGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A