MyabA.00309.a
Putative acyl-CoA dehydrogenase
| CENTER ID: | MyabA.00309.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00309.a.A1.GE30286 | Full length( MyabA.00309.a ) | 1 | 411 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00309.a.A1.PS01029 | Full length( MyabA.00309.a ) | 1 | 411 |
Structures
4HR3
DEPOSITED: 10/26/2012
DETERMINATION: XRay
CLONE: MyabA.00309.a.A1.GE30286
PROTEIN: MyabA.00309.a.A1.PS01029
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129853 |
| PATRIC ID: | fig|36809.5.peg.285 |
| RefSeq: | YP_001701009.1 |
| UniProt: | B1MFF5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFAPSARAA ELIAAVREFI DAEVMPVERA VLAHHDELLG ARAGTTAELW HVPPELDSLK AKARAAGLWN LFLPDPELGG GLSNSEYAPL AEQMGRSLFA PTVFNCNAPD SGNMEVLHRY GSQEQKEVWL EPLLEGDIRS AFCMTEPDVA SSDATNMAAT AVVEGDEVVI NGRKWWSTGV GHPDCKVIIF MGLTDPNAHR YARHSMVLVP MDTPGITVER MLPTMGFYDE PGGHGVVSFD NVRLPADAFI AGPGKGFEIA QGRLGPGRVH HAMRLIGLAE VALEHACRRG LDRTAFGKPL VNLGGNRERI ADARIAINQT RLLVLHAAWL LDTVGIMGAL SAVSEIKVAA PNMAQQVIDM AIQIHGGGGL SNDFPLAAAW VNARALRLAD GPDEVHRGVV ARIELAKYAN D
NT Sequence
ATGGACTTCG CCCCGTCTGC CCGCGCCGCC GAATTGATCG CCGCCGTCCG AGAGTTCATC GACGCAGAGG TCATGCCGGT AGAACGTGCG GTGTTGGCCC ACCATGACGA GCTGCTGGGT GCACGCGCGG GTACGACCGC CGAACTGTGG CACGTGCCCC CCGAGCTGGA TTCACTCAAG GCGAAAGCGC GTGCTGCCGG ATTGTGGAAT CTGTTTCTGC CCGATCCCGA ATTGGGTGGC GGGCTATCCA ACTCCGAATA CGCGCCGCTG GCCGAACAGA TGGGTCGTTC GCTGTTCGCG CCCACGGTAT TCAACTGCAA CGCACCTGAT TCCGGAAACA TGGAGGTACT GCACCGATAC GGCAGTCAGG AGCAGAAAGA GGTATGGCTC GAACCACTGT TGGAGGGCGA TATCCGGTCC GCGTTCTGCA TGACCGAACC CGATGTCGCA TCTTCCGATG CCACGAACAT GGCAGCCACT GCCGTCGTCG AGGGCGACGA GGTGGTGATC AACGGGCGTA AGTGGTGGAG CACCGGCGTC GGCCACCCGG ACTGCAAGGT GATCATCTTC ATGGGTCTGA CGGACCCCAA CGCGCATCGG TACGCCCGCC ACTCCATGGT TTTGGTCCCG ATGGACACCC CGGGTATCAC CGTTGAGCGC ATGCTGCCCA CCATGGGCTT TTACGACGAG CCAGGCGGAC ACGGGGTGGT GTCGTTCGAC AATGTGCGGC TCCCGGCCGA TGCGTTCATC GCCGGGCCGG GCAAGGGATT CGAGATCGCT CAGGGAAGGC TGGGGCCTGG GCGCGTGCAC CATGCGATGC GGCTGATCGG TCTGGCCGAG GTGGCGTTGG AGCACGCGTG CCGACGAGGC CTGGACCGCA CCGCCTTTGG CAAACCGTTG GTAAACCTGG GCGGTAACCG TGAGCGCATC GCCGATGCGC GCATCGCCAT CAACCAGACG CGGCTACTGG TGCTGCATGC CGCCTGGCTG CTGGACACCG TGGGCATCAT GGGAGCGCTG TCGGCGGTGT CCGAGATCAA GGTGGCCGCC CCCAACATGG CCCAGCAGGT CATTGACATG GCGATCCAAA TACACGGTGG TGGAGGGCTT TCCAATGACT TTCCGCTGGC AGCCGCCTGG GTGAACGCGC GAGCGCTGCG GCTGGCCGAC GGGCCCGACG AGGTGCACCG CGGCGTGGTG GCCCGAATTG AGTTGGCGAA ATACGCTAAC GAC
Details for MyabA.00309.a.A1.GE30286
| HARVESTED ON: | 8/31/2010 |
| SEQUENCED ON: | 9/7/2010 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 61 |
Validated AA Sequence
MTEPDVAXSD ATNMAATAVV EGDEVVINGR KWWSTGVGHP DCKVIIFMXL TDPNAHRYAR HSMVLVPMDT PGITVERMLP TMGFYDEPGG HGVVSFDNVR LPADAFIAGP GKGFEIAQGR LGPGRVHHAM RLIGLAEVAL EHACRRGLDR TAFGKPLVNL GGNRERIADA RIAINQTRLL VLHAAWLLDT VGIMGALSAV SEIKVAAPNM AQQVIDMAIQ IHGGGGLSND FPLAAAWVNA RALRLADGPD EVHRGVVARI ELAKYA
Validated NT Sequence
tagcgtattt cgccaactca attcgggcca ccacgccgcg gtgcacctcg tcgggcccgt cggccagccg cagcgctcgc gcgttcaccc aggcggctgc cagcggaaag tcattggaaa gccctccacc accgtgtatt tggatcgcca tgtcaatgac ctgctgggcc atgttggggg cggccacctt gatctcggac accgccgaca gcgctcccat gatgcccacg gtgtccagca gccaggcggc atgcagcacc agtagccgcg tctggttgat ggcgatgcgc gcatcggcga tgcgctcacg gttaccgccc aggtttacca acggtttgcc aaaggcggtg cggtccaggc ctcgtcggca cgcgtgctcc aacgccacct cggccagacc gatcagccgc atcgcatggt gcacgcgccc aggccccagc cttccctgag cgatctcgaa tcccttgccc ggcccggcga tgaacgcatc ggccgggagc cgcacattgt cgaacgacac caccccgtgt ccgcctggct cgtcgtaaaa gcccatggtg ggcagcatgc gctcaacggt gatacccggg gtgtccatcg ggaccaaaac catggagtgg cgggcgtacc gatgcgcgtt ggggtccgtc aganccatga agatgatcac cttgcagtcc gggtggccga cgccggtgct ccaccactta cgcccgttga tcaccacctc gtcgccctcg acgacngcng tggctgccat gttcgtggca tcggaanatg cgacatcggg ttcggtcatg canaacgcgg ancggatatc gccctnnnac agnggntcga gccatacctc tttctgctnc ngactgccgt atc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFAPSARA AELIAAVREF IDAEVMPVER AVLAHHDELL GARAGTTAEL WHVPPELDSL KAKARAAGLW NLFLPDPELG GGLSNSEYAP LAEQMGRSLF APTVFNCNAP DSGNMEVLHR YGSQEQKEVW LEPLLEGDIR SAFCMTEPDV ASSDATNMAA TAVVEGDEVV INGRKWWSTG VGHPDCKVII FMGLTDPNAH RYARHSMVLV PMDTPGITVE RMLPTMGFYD EPGGHGVVSF DNVRLPADAF IAGPGKGFEI AQGRLGPGRV HHAMRLIGLA EVALEHACRR GLDRTAFGKP LVNLGGNRER IADARIAINQ TRLLVLHAAW LLDTVGIMGA LSAVSEIKVA APNMAQQVID MAIQIHGGGG LSNDFPLAAA WVNARALRLA DGPDEVHRGV VARIELAKYA ND
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacttcgcc ccgtctgccc gcgccgccga attgatcgcc gccgtccgag agttcatcga cgcagaggtc atgccggtag aacgtgcggt gttggcccac catgacgagc tgctgggtgc acgcgcgggt acgaccgccg aactgtggca cgtgcccccc gagctggatt cactcaaggc gaaagcgcgt gctgccggat tgtggaatct gtttctgccc gatcccgaat tgggtggcgg gctatccaac tccgaatacg cgccgctggc cgaacagatg ggtcgttcgc tgttcgcgcc cacggtattc aactgcaacg cacctgattc cggaaacatg gaggtactgc accgatacgg cagtcaggag cagaaagagg tatggctcga accactgttg gagggcgata tccggtccgc gttctgcatg accgaacccg atgtcgcatc ttccgatgcc acgaacatgg cagccactgc cgtcgtcgag ggcgacgagg tggtgatcaa cgggcgtaag tggtggagca ccggcgtcgg ccacccggac tgcaaggtga tcatcttcat gggtctgacg gaccccaacg cgcatcggta cgcccgccac tccatggttt tggtcccgat ggacaccccg ggtatcaccg ttgagcgcat gctgcccacc atgggctttt acgacgagcc aggcggacac ggggtggtgt cgttcgacaa tgtgcggctc ccggccgatg cgttcatcgc cgggccgggc aagggattcg agatcgctca gggaaggctg gggcctgggc gcgtgcacca tgcgatgcgg ctgatcggtc tggccgaggt ggcgttggag cacgcgtgcc gacgaggcct ggaccgcacc gcctttggca aaccgttggt aaacctgggc ggtaaccgtg agcgcatcgc cgatgcgcgc atcgccatca accagacgcg gctactggtg ctgcatgccg cctggctgct ggacaccgtg ggcatcatgg gagcgctgtc ggcggtgtcc gagatcaagg tggccgcccc caacatggcc cagcaggtca ttgacatggc gatccaaata cacggtggtg gagggctttc caatgacttt ccgctggcag ccgcctgggt gaacgcgcga gcgctgcggc tggccgacgg gcccgacgag gtgcaccgcg gcgtggtggc ccgaattgag ttggcgaaat acgctaacga caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.00309.a.A1.PS01029
| PURIFICATION DATe: | 4/6/2011 |
| CONCENTRATION: | 53.09mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 61 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTEPDVAXSD ATNMAATAVV EGDEVVINGR KWWSTGVGHP DCKVIIFMXL TDPNAHRYAR HSMVLVPMDT PGITVERMLP TMGFYDEPGG HGVVSFDNVR LPADAFIAGP GKGFEIAQGR LGPGRVHHAM RLIGLAEVAL EHACRRGLDR TAFGKPLVNL GGNRERIADA RIAINQTRLL VLHAAWLLDT VGIMGALSAV SEIKVAAPNM AQQVIDMAIQ IHGGGGLSND FPLAAAWVNA RALRLADGPD EVHRGVVARI ELAKYA
Validated NT Sequence
tagcgtattt cgccaactca attcgggcca ccacgccgcg gtgcacctcg tcgggcccgt cggccagccg cagcgctcgc gcgttcaccc aggcggctgc cagcggaaag tcattggaaa gccctccacc accgtgtatt tggatcgcca tgtcaatgac ctgctgggcc atgttggggg cggccacctt gatctcggac accgccgaca gcgctcccat gatgcccacg gtgtccagca gccaggcggc atgcagcacc agtagccgcg tctggttgat ggcgatgcgc gcatcggcga tgcgctcacg gttaccgccc aggtttacca acggtttgcc aaaggcggtg cggtccaggc ctcgtcggca cgcgtgctcc aacgccacct cggccagacc gatcagccgc atcgcatggt gcacgcgccc aggccccagc cttccctgag cgatctcgaa tcccttgccc ggcccggcga tgaacgcatc ggccgggagc cgcacattgt cgaacgacac caccccgtgt ccgcctggct cgtcgtaaaa gcccatggtg ggcagcatgc gctcaacggt gatacccggg gtgtccatcg ggaccaaaac catggagtgg cgggcgtacc gatgcgcgtt ggggtccgtc aganccatga agatgatcac cttgcagtcc gggtggccga cgccggtgct ccaccactta cgcccgttga tcaccacctc gtcgccctcg acgacngcng tggctgccat gttcgtggca tcggaanatg cgacatcggg ttcggtcatg canaacgcgg ancggatatc gccctnnnac agnggntcga gccatacctc tttctgctnc ngactgccgt atc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFAPSARA AELIAAVREF IDAEVMPVER AVLAHHDELL GARAGTTAEL WHVPPELDSL KAKARAAGLW NLFLPDPELG GGLSNSEYAP LAEQMGRSLF APTVFNCNAP DSGNMEVLHR YGSQEQKEVW LEPLLEGDIR SAFCMTEPDV ASSDATNMAA TAVVEGDEVV INGRKWWSTG VGHPDCKVII FMGLTDPNAH RYARHSMVLV PMDTPGITVE RMLPTMGFYD EPGGHGVVSF DNVRLPADAF IAGPGKGFEI AQGRLGPGRV HHAMRLIGLA EVALEHACRR GLDRTAFGKP LVNLGGNRER IADARIAINQ TRLLVLHAAW LLDTVGIMGA LSAVSEIKVA APNMAQQVID MAIQIHGGGG LSNDFPLAAA WVNARALRLA DGPDEVHRGV VARIELAKYA ND
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACTTCGCC CCGTCTGCCC GCGCCGCCGA ATTGATCGCC GCCGTCCGAG AGTTCATCGA CGCAGAGGTC ATGCCGGTAG AACGTGCGGT GTTGGCCCAC CATGACGAGC TGCTGGGTGC ACGCGCGGGT ACGACCGCCG AACTGTGGCA CGTGCCCCCC GAGCTGGATT CACTCAAGGC GAAAGCGCGT GCTGCCGGAT TGTGGAATCT GTTTCTGCCC GATCCCGAAT TGGGTGGCGG GCTATCCAAC TCCGAATACG CGCCGCTGGC CGAACAGATG GGTCGTTCGC TGTTCGCGCC CACGGTATTC AACTGCAACG CACCTGATTC CGGAAACATG GAGGTACTGC ACCGATACGG CAGTCAGGAG CAGAAAGAGG TATGGCTCGA ACCACTGTTG GAGGGCGATA TCCGGTCCGC GTTCTGCATG ACCGAACCCG ATGTCGCATC TTCCGATGCC ACGAACATGG CAGCCACTGC CGTCGTCGAG GGCGACGAGG TGGTGATCAA CGGGCGTAAG TGGTGGAGCA CCGGCGTCGG CCACCCGGAC TGCAAGGTGA TCATCTTCAT GGGTCTGACG GACCCCAACG CGCATCGGTA CGCCCGCCAC TCCATGGTTT TGGTCCCGAT GGACACCCCG GGTATCACCG TTGAGCGCAT GCTGCCCACC ATGGGCTTTT ACGACGAGCC AGGCGGACAC GGGGTGGTGT CGTTCGACAA TGTGCGGCTC CCGGCCGATG CGTTCATCGC CGGGCCGGGC AAGGGATTCG AGATCGCTCA GGGAAGGCTG GGGCCTGGGC GCGTGCACCA TGCGATGCGG CTGATCGGTC TGGCCGAGGT GGCGTTGGAG CACGCGTGCC GACGAGGCCT GGACCGCACC GCCTTTGGCA AACCGTTGGT AAACCTGGGC GGTAACCGTG AGCGCATCGC CGATGCGCGC ATCGCCATCA ACCAGACGCG GCTACTGGTG CTGCATGCCG CCTGGCTGCT GGACACCGTG GGCATCATGG GAGCGCTGTC GGCGGTGTCC GAGATCAAGG TGGCCGCCCC CAACATGGCC CAGCAGGTCA TTGACATGGC GATCCAAATA CACGGTGGTG GAGGGCTTTC CAATGACTTT CCGCTGGCAG CCGCCTGGGT GAACGCGCGA GCGCTGCGGC TGGCCGACGG GCCCGACGAG GTGCACCGCG GCGTGGTGGC CCGAATTGAG TTGGCGAAAT ACGCTAACGA CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA