SSGCID: Target Page

MymaA.00309.a

Acyl-CoA dehydrogenase FadE2_1

CENTER ID:	MymaA.00309.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00309.a.A1.GE29990	Full length( MymaA.00309.a )		1	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00309.a.A1.PS00825	Full length( MymaA.00309.a )		1	407

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.190
RefSeq:	YP_001848481.1
UniProt:	B2HJF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLFDISDRA KQYQSDLLEF MDSQVYPAEA IYDEQMRAAG DPHFHPPILE ELKAAARNRG LWNLFHPHPE WGPGLTNLEY APLAEILGRS HIASEACNCS APDTGNMEVL TLFGTDEHQQ RYLKPLLEGK IRSAFAMTEP AVASSDATNV EMSMVRDGDS YILNGRKWFA SNGMHRNCKV LIVMGKTDPT APVHRQQSMM VVPIDAPGLT VRRNLPVFGY QDREGHAEID FVDVRVPVKD VLKGEGEGFA ISQARLGPGR IHHCMRAIGM AERALELMCK RAQSRVTFGK PISENANVRD WIAEARIEIE MIRLLTLKAA YLMDTVGNKQ ARTEIAAIKV AAPNIALKIV DRAIQVHGGG GVTDDFPLAM AWAHLRTLRL ADGPDEVHKR AIATQELRKY RNGAHGS

NT Sequence

ATGTCACTGT TCGATATTTC CGATCGCGCC AAGCAGTACC AATCAGACCT GCTCGAGTTC ATGGATTCCC AGGTGTACCC GGCTGAGGCC ATCTACGACG AACAGATGCG CGCCGCCGGC GACCCGCATT TCCATCCTCC GATCTTGGAG GAACTCAAGG CCGCGGCCCG AAATCGCGGG CTGTGGAACC TGTTTCACCC GCACCCCGAG TGGGGGCCGG GATTGACCAA CCTCGAGTAC GCGCCATTGG CGGAGATCCT GGGCCGCAGC CACATCGCCT CGGAGGCCTG CAACTGTAGC GCCCCGGACA CCGGCAACAT GGAGGTGCTG ACCCTCTTCG GCACCGATGA GCACCAGCAG CGCTACCTCA AGCCGCTGCT AGAGGGCAAA ATCCGCTCTG CCTTCGCCAT GACCGAGCCC GCGGTGGCCA GCTCCGATGC GACGAACGTC GAGATGTCGA TGGTGCGCGA CGGTGACAGC TACATCCTCA ACGGGCGAAA ATGGTTTGCG TCCAACGGCA TGCACCGTAA TTGCAAAGTC TTGATCGTGA TGGGTAAGAC CGACCCCACC GCGCCGGTGC ACCGCCAGCA ATCGATGATG GTGGTGCCGA TCGATGCGCC GGGCCTCACC GTGCGGCGCA ACCTGCCCGT CTTTGGCTAC CAGGACCGCG AAGGACATGC CGAAATCGAC TTCGTCGACG TACGGGTGCC GGTCAAAGAC GTGCTCAAGG GCGAAGGCGA GGGATTTGCC ATCAGCCAGG CCCGGCTGGG CCCCGGCCGC ATTCATCACT GCATGCGCGC GATCGGCATG GCGGAGCGGG CGCTGGAGTT GATGTGCAAA CGAGCCCAAT CCCGGGTCAC CTTCGGCAAG CCCATCAGCG AGAACGCGAA CGTGCGCGAC TGGATCGCCG AGGCCCGGAT CGAGATCGAG ATGATCCGAT TGCTGACCCT CAAGGCCGCC TACCTGATGG ATACGGTCGG AAACAAGCAG GCCCGTACCG AGATTGCGGC GATCAAGGTG GCGGCCCCCA ACATCGCACT CAAAATCGTC GACCGGGCCA TTCAGGTGCA CGGCGGCGGC GGGGTCACCG ACGACTTTCC GCTGGCGATG GCCTGGGCCC ACCTGCGCAC CTTGCGGCTG GCCGACGGCC CAGACGAGGT GCACAAACGA GCGATTGCCA CCCAAGAGTT GCGCAAGTAC CGCAACGGCG CCCACGGATC G

Details for MymaA.00309.a.A1.GE29990

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/18/2010
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MSLFDISDRA KQYQSDLLEF MDSQVYPAEA IYDEQMRAAG DPHFHPXXXE ELKAAARNRG LWNLFHPHPE WGPGLTNLEY APLAEILGRS HIASEACNCS APDTGNMEVL TLFGTDEHQQ RYLKPLLEGK IRSAFAMTEP AVASSDATNV EMSMVRDGDS YILNGRKWFA SNGMHRNCKV LIVMGKTDPT APVHRQQSMM VVPIDAPGLT VRRNLPVFGY QDREGHAEID FVDVRVPVKD VLKGEGEGFA ISQARLGPGR IHHCMRAIGM AERALELMCK RAQSRVTFGK PISENANVRD WIAEARIEIE MIRLLTLKAA YLMDTVGNKQ ARTEIAAIKV AAPNIALKIV DRAIQVHGGG GVTDDFPLAM AWAHLRTLRL ADGPDEVHKR AIATQELRKY RNG

Validated NT Sequence

gcgccgttgc ggtacttgcg caactcttgg gtggcaatcg ctcgtttgtg cacctcgtct gggccgtcgg ccagccgcaa ggtgcgcagg tgggcccagg ccatcgccag cggaaagtcg tcggtgaccc cgccgccgcc gtgcacctga atggcccggt cgacgatttt gagtgcgatg ttgggggccg ccaccttgat cgccgcaatc tcggtacggg cctgcttgtt tccgaccgta tccatcaggt aggcggcctt gagggtcagc aatcggatca tctcgatctc gatccgggcc tcggcgatcc agtcgcgcac gttcgcgttc tcgctgatgg gcttgccgaa ggtgacccgg gattgggctc gtttgcacat caactccagc gcccgctccg ccatgccgat cgcgcgcatg cagtgatgaa tgcggccggg gcccagccgg gcctggctga tggcaaatcc ctcgccttcg cccttgagca cgtctttgac cggcacccgt acgtcgacga agtcgatttc ggcatgtcct tcgcggtcct ggtagccaaa gacgggcagg ttgcgccgca cggtgaggcc cggcgcatcg atcggcacca ccatcatcga ttgctggcgg tgcaccggcg cggtggggtc ggtcttaccc atcacgatca agactttgca attacggtgc atgccgttgg acgcaaacca ttttcgcccg ttgaggatgt agctgtcacc gtcgcgcacc atcgacatct cgacgttcgt cgcatcggag ctggccaccg cgggctcggt catggcgaag gcagagcgga ttttgccctc tagcagcggc ttgaggtagc gctgctggtg ctcatcggtg ccgaagaggg tcagcacctc catgttgccg gtgtccgggg cgctacagtt gcaggcctcc gaggcgatgt ggctgcggcc caggatctcc gccaatggcg cgtactcgag gttggtcaat cccggccccc actcggggtg cgggtgaaac aggttccaca gcccgcgatt tcgggccgcg gccttgagtt cctccnnnnn nngaggatgg aaatgcgggt cgccggcggc gcgcatctgt tcgtcgtaga tggcctcagc cgggtacacc tgggaatcca tgaactcgag caggtctgat tggtactgct tggcgcgatc ggaaatatcg aacagtgaca tcgaaccagg accctgggtc tgagcttcca gggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLFDISDR AKQYQSDLLE FMDSQVYPAE AIYDEQMRAA GDPHFHPPIL EELKAAARNR GLWNLFHPHP EWGPGLTNLE YAPLAEILGR SHIASEACNC SAPDTGNMEV LTLFGTDEHQ QRYLKPLLEG KIRSAFAMTE PAVASSDATN VEMSMVRDGD SYILNGRKWF ASNGMHRNCK VLIVMGKTDP TAPVHRQQSM MVVPIDAPGL TVRRNLPVFG YQDREGHAEI DFVDVRVPVK DVLKGEGEGF AISQARLGPG RIHHCMRAIG MAERALELMC KRAQSRVTFG KPISENANVR DWIAEARIEI EMIRLLTLKA AYLMDTVGNK QARTEIAAIK VAAPNIALKI VDRAIQVHGG GGVTDDFPLA MAWAHLRTLR LADGPDEVHK RAIATQELRK YRNGAHG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcactgttc gatatttccg atcgcgccaa gcagtaccaa tcagacctgc tcgagttcat ggattcccag gtgtacccgg ctgaggccat ctacgacgaa cagatgcgcg ccgccggcga cccgcatttc catcctccga tcttggagga actcaaggcc gcggcccgaa atcgcgggct gtggaacctg tttcacccgc accccgagtg ggggccggga ttgaccaacc tcgagtacgc gccattggcg gagatcctgg gccgcagcca catcgcctcg gaggcctgca actgtagcgc cccggacacc ggcaacatgg aggtgctgac cctcttcggc accgatgagc accagcagcg ctacctcaag ccgctgctag agggcaaaat ccgctctgcc ttcgccatga ccgagcccgc ggtggccagc tccgatgcga cgaacgtcga gatgtcgatg gtgcgcgacg gtgacagcta catcctcaac gggcgaaaat ggtttgcgtc caacggcatg caccgtaatt gcaaagtctt gatcgtgatg ggtaagaccg accccaccgc gccggtgcac cgccagcaat cgatgatggt ggtgccgatc gatgcgccgg gcctcaccgt gcggcgcaac ctgcccgtct ttggctacca ggaccgcgaa ggacatgccg aaatcgactt cgtcgacgta cgggtgccgg tcaaagacgt gctcaagggc gaaggcgagg gatttgccat cagccaggcc cggctgggcc ccggccgcat tcatcactgc atgcgcgcga tcggcatggc ggagcgggcg ctggagttga tgtgcaaacg agcccaatcc cgggtcacct tcggcaagcc catcagcgag aacgcgaacg tgcgcgactg gatcgccgag gcccggatcg agatcgagat gatccgattg ctgaccctca aggccgccta cctgatggat acggtcggaa acaagcaggc ccgtaccgag attgcggcga tcaaggtggc ggcccccaac atcgcactca aaatcgtcga ccgggccatt caggtgcacg gcggcggcgg ggtcaccgac gactttccgc tggcgatggc ctgggcccac ctgcgcacct tgcggctggc cgacggccca gacgaggtgc acaaacgagc gattgccacc caagagttgc gcaagtaccg caacggcgcc cacggaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MymaA.00309.a.A1.PS00825

PURIFICATION DATe:	10/22/2010
CONCENTRATION:	32.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCACTGTTC GATATTTCCG ATCGCGCCAA GCAGTACCAA TCAGACCTGC TCGAGTTCAT GGATTCCCAG GTGTACCCGG CTGAGGCCAT CTACGACGAA CAGATGCGCG CCGCCGGCGA CCCGCATTTC CATCCTCCGA TCTTGGAGGA ACTCAAGGCC GCGGCCCGAA ATCGCGGGCT GTGGAACCTG TTTCACCCGC ACCCCGAGTG GGGGCCGGGA TTGACCAACC TCGAGTACGC GCCATTGGCG GAGATCCTGG GCCGCAGCCA CATCGCCTCG GAGGCCTGCA ACTGTAGCGC CCCGGACACC GGCAACATGG AGGTGCTGAC CCTCTTCGGC ACCGATGAGC ACCAGCAGCG CTACCTCAAG CCGCTGCTAG AGGGCAAAAT CCGCTCTGCC TTCGCCATGA CCGAGCCCGC GGTGGCCAGC TCCGATGCGA CGAACGTCGA GATGTCGATG GTGCGCGACG GTGACAGCTA CATCCTCAAC GGGCGAAAAT GGTTTGCGTC CAACGGCATG CACCGTAATT GCAAAGTCTT GATCGTGATG GGTAAGACCG ACCCCACCGC GCCGGTGCAC CGCCAGCAAT CGATGATGGT GGTGCCGATC GATGCGCCGG GCCTCACCGT GCGGCGCAAC CTGCCCGTCT TTGGCTACCA GGACCGCGAA GGACATGCCG AAATCGACTT CGTCGACGTA CGGGTGCCGG TCAAAGACGT GCTCAAGGGC GAAGGCGAGG GATTTGCCAT CAGCCAGGCC CGGCTGGGCC CCGGCCGCAT TCATCACTGC ATGCGCGCGA TCGGCATGGC GGAGCGGGCG CTGGAGTTGA TGTGCAAACG AGCCCAATCC CGGGTCACCT TCGGCAAGCC CATCAGCGAG AACGCGAACG TGCGCGACTG GATCGCCGAG GCCCGGATCG AGATCGAGAT GATCCGATTG CTGACCCTCA AGGCCGCCTA CCTGATGGAT ACGGTCGGAA ACAAGCAGGC CCGTACCGAG ATTGCGGCGA TCAAGGTGGC GGCCCCCAAC ATCGCACTCA AAATCGTCGA CCGGGCCATT CAGGTGCACG GCGGCGGCGG GGTCACCGAC GACTTTCCGC TGGCGATGGC CTGGGCCCAC CTGCGCACCT TGCGGCTGGC CGACGGCCCA GACGAGGTGC ACAAACGAGC GATTGCCACC CAAGAGTTGC GCAAGTACCG CAACGGCGCC CACGGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA