MyabA.00309.b

Putative acyl-CoA dehydrogenase

CENTER ID: MyabA.00309.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00309.b.A1.GE30287 Full length( MyabA.00309.b ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00309.b.A1.PS00892 Full length( MyabA.00309.b ) 1 393

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.357
RefSeq: YP_001701081.1
UniProt: B1MFM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDFSIPEEL AAKAQRVREF VTETVIPYER DPRLTAHGPT EELRDELVGK ARAAGLLTVQ APESYGGWGL SHIGQAVIFE AAGWSTLGPI AMNCAAPDEG NMFLLGKITN EEQSEKFLRP VIEGYQRSAF AMTEPDGAGS DPSQLKTSAT FDGQNFVING RKWLITGARG AKTWIIMANL EANDHLPEGP TLFLCDGETE GIVIERIMNT MDRNYAEGHA VVRFDNLTLP REALLGETGQ AFRYAQLRLA PARLTHCMRW LGAASRAQDI AVDYARTRTS FGKTIGEHQG VSFMLADNEI ALHQCRLTIW HACWMMDNGA KGRHESSMAK SFVSEELFKV TDRCVQVLGG IGISDETPVE MIFRDMRAFR LYDGPTEVHK YAIGRQVLRT PRA
NT Sequence
ATGATCGACT TCAGCATTCC GGAAGAGCTG GCCGCGAAGG CGCAGCGGGT GCGTGAGTTC GTCACCGAGA CCGTGATTCC GTACGAGCGC GATCCTCGCC TGACCGCGCA CGGACCCACC GAGGAGCTGC GCGACGAGTT GGTCGGTAAG GCCAGGGCGG CCGGTCTGCT GACCGTTCAG GCGCCAGAAT CCTATGGTGG GTGGGGTCTT TCGCACATCG GCCAGGCGGT GATCTTCGAG GCCGCGGGCT GGTCGACCCT GGGGCCCATC GCCATGAACT GTGCGGCACC CGATGAGGGC AACATGTTCC TGCTCGGCAA GATCACCAAC GAGGAACAAT CCGAGAAGTT TCTGCGTCCC GTCATCGAGG GGTATCAGCG CTCGGCGTTC GCGATGACCG AACCCGACGG GGCCGGTTCC GATCCGTCGC AGTTGAAGAC CTCGGCGACC TTCGACGGGC AGAATTTCGT CATCAACGGC CGTAAATGGC TCATCACCGG AGCTCGCGGC GCCAAAACGT GGATCATCAT GGCCAACCTG GAGGCCAACG ATCACCTGCC GGAGGGGCCG ACACTGTTTC TCTGCGACGG CGAGACCGAA GGCATTGTGA TCGAACGCAT CATGAACACC ATGGACCGCA ACTACGCCGA GGGGCATGCC GTGGTGCGGT TCGACAATCT CACCCTGCCG CGGGAGGCGT TGCTCGGTGA GACCGGGCAG GCGTTCCGAT ACGCGCAGCT GCGGCTGGCC CCGGCCCGGT TGACGCACTG TATGCGCTGG CTCGGTGCCG CCTCCCGGGC CCAGGACATC GCGGTCGACT ATGCGCGAAC CCGGACCTCG TTCGGTAAGA CGATCGGCGA GCACCAGGGG GTTTCCTTCA TGCTCGCCGA CAACGAGATC GCGTTGCATC AGTGTCGACT GACCATCTGG CATGCCTGCT GGATGATGGA CAACGGCGCC AAGGGCCGGC ACGAAAGTTC GATGGCGAAG TCCTTCGTGT CCGAGGAGTT GTTCAAGGTG ACCGACCGGT GTGTTCAGGT GCTCGGCGGC ATCGGCATCA GCGATGAGAC GCCGGTGGAG ATGATCTTCC GTGATATGCG CGCCTTCCGT CTGTACGACG GTCCCACCGA GGTGCACAAG TACGCCATCG GACGCCAGGT GCTTCGGACT CCGCGCGCC
Details for MyabA.00309.b.A1.GE30287
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MIDFSIPEEL AAKAQRVREF VTETVIPYER DPRLTAHGPT EELRDELVGK ARAAGLLTVQ APESYGGWGL SHIGQAVIFE AAGWSTLGPI AMNCAAPDEG NMFLLGKITN EEQSEKFLRP VIEGYQRSAF AMTEPDGAGS DPSQLKTSAT FDGQNFVING RKWLITGARG AKTWIIMANL EANDHLPEGP TLFLCDGETE GIVIERIMNT MDRNYAEGHA VVRFDNLTLP REALLGETGQ AFRYAQLRLA PARLTHCMRW LGAASRAQDI AVDYARTRTS FGKTIGEHQG VSFMLADNEI ALHQCRLTIW HACWMMDNGA KGRHESSMAK SFVSEELFKV TDRCVQVLGG IGISDETPVE MIFRDMRAFR LYDGPTEVHK YAIGRQVLRT PRA
Validated NT Sequence
aggcgcgcgg agtccgaagc acctggcgtc cgatggcgta cttgtgcacc tcggtgggac cgtcgtacag acggaaggcg cgcatatcac ggaagatcat ctccaccggc gtctcatcgc tgatgccgat gccgccgagc acctgaacac accggtcggt caccttgaac aactcctcgg acacgaagga cttcgccatc gaactttcgt gccggccctt ggcgccgttg tccatcatcc agcaggcatg ccagatggtc agtcgacact gatgcaacgc gatctcgttg tcggcgagca tgaaggaaac cccctggtgc tcgccgatcg tcttaccgaa cgaggtccgg gttcgcgcat agtcgaccgc gatgtcctgg gcccgggagg cggcaccgag ccagcgcata cagtgcgtca accgggccgg ggccagccgc agctgcgcgt atcggaacgc ctgcccggtc tcaccgagca acgcctcccg cggcagggtg agattgtcga accgcaccac ggcatgcccc tcggcgtagt tgcggtccat ggtgttcatg atgcgttcga tcacaatgcc ttcggtctcg ccgtcgcaga gaaacagtgt cggcccctcc ggcaggtgat cgttggcctc caggttggcc atgatgatcc acgttttggc gccgcgagct ccggtgatga gccatttacg gccgttgatg acgaaattct gcccgtcgaa ggtcgccgag gtcttcaact gcgacggatc ggaaccggcc ccgtcgggtt cggtcatcgc gaacgccgag cgctgatacc cctcgatgac gggacgcaga aacttctcgg attgttcctc gttggtgatc ttgccgagca ggaacatgtt gccctcatcg ggtgccgcac agttcatggc gatgggcccc agggtcgacc agcccgcggc ctcgaagatc accgcctggc cgatgtgcga aagaccccac ccaccatagg attctggcgc ctgaacggtc agcagaccgg ccgccctggc cttaccgacc aactcgtcgc gcagctcctc ggtgggtccg tgcgcggtca ggcgaggatc gcgctcgtac ggaatcacgg tctcggtgac gaactcacgc acccgctgcg ccttcgcggc cagctcttcc ggaatgctga agtcgatcat cgaaccagga ccctgggtct gagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDFSIPEE LAAKAQRVRE FVTETVIPYE RDPRLTAHGP TEELRDELVG KARAAGLLTV QAPESYGGWG LSHIGQAVIF EAAGWSTLGP IAMNCAAPDE GNMFLLGKIT NEEQSEKFLR PVIEGYQRSA FAMTEPDGAG SDPSQLKTSA TFDGQNFVIN GRKWLITGAR GAKTWIIMAN LEANDHLPEG PTLFLCDGET EGIVIERIMN TMDRNYAEGH AVVRFDNLTL PREALLGETG QAFRYAQLRL APARLTHCMR WLGAASRAQD IAVDYARTRT SFGKTIGEHQ GVSFMLADNE IALHQCRLTI WHACWMMDNG AKGRHESSMA KSFVSEELFK VTDRCVQVLG GIGISDETPV EMIFRDMRAF RLYDGPTEVH KYAIGRQVLR TPRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgacttc agcattccgg aagagctggc cgcgaaggcg cagcgggtgc gtgagttcgt caccgagacc gtgattccgt acgagcgcga tcctcgcctg accgcgcacg gacccaccga ggagctgcgc gacgagttgg tcggtaaggc cagggcggcc ggtctgctga ccgttcaggc gccagaatcc tatggtgggt ggggtctttc gcacatcggc caggcggtga tcttcgaggc cgcgggctgg tcgaccctgg ggcccatcgc catgaactgt gcggcacccg atgagggcaa catgttcctg ctcggcaaga tcaccaacga ggaacaatcc gagaagtttc tgcgtcccgt catcgagggg tatcagcgct cggcgttcgc gatgaccgaa cccgacgggg ccggttccga tccgtcgcag ttgaagacct cggcgacctt cgacgggcag aatttcgtca tcaacggccg taaatggctc atcaccggag ctcgcggcgc caaaacgtgg atcatcatgg ccaacctgga ggccaacgat cacctgccgg aggggccgac actgtttctc tgcgacggcg agaccgaagg cattgtgatc gaacgcatca tgaacaccat ggaccgcaac tacgccgagg ggcatgccgt ggtgcggttc gacaatctca ccctgccgcg ggaggcgttg ctcggtgaga ccgggcaggc gttccgatac gcgcagctgc ggctggcccc ggcccggttg acgcactgta tgcgctggct cggtgccgcc tcccgggccc aggacatcgc ggtcgactat gcgcgaaccc ggacctcgtt cggtaagacg atcggcgagc accagggggt ttccttcatg ctcgccgaca acgagatcgc gttgcatcag tgtcgactga ccatctggca tgcctgctgg atgatggaca acggcgccaa gggccggcac gaaagttcga tggcgaagtc cttcgtgtcc gaggagttgt tcaaggtgac cgaccggtgt gttcaggtgc tcggcggcat cggcatcagc gatgagacgc cggtggagat gatcttccgt gatatgcgcg ccttccgtct gtacgacggt cccaccgagg tgcacaagta cgccatcgga cgccaggtgc ttcggactcc gcgcgccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyabA.00309.b.A1.PS00892
PURIFICATION DATe: 12/7/2010
CONCENTRATION: 48.44mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MIDFSIPEEL AAKAQRVREF VTETVIPYER DPRLTAHGPT EELRDELVGK ARAAGLLTVQ APESYGGWGL SHIGQAVIFE AAGWSTLGPI AMNCAAPDEG NMFLLGKITN EEQSEKFLRP VIEGYQRSAF AMTEPDGAGS DPSQLKTSAT FDGQNFVING RKWLITGARG AKTWIIMANL EANDHLPEGP TLFLCDGETE GIVIERIMNT MDRNYAEGHA VVRFDNLTLP REALLGETGQ AFRYAQLRLA PARLTHCMRW LGAASRAQDI AVDYARTRTS FGKTIGEHQG VSFMLADNEI ALHQCRLTIW HACWMMDNGA KGRHESSMAK SFVSEELFKV TDRCVQVLGG IGISDETPVE MIFRDMRAFR LYDGPTEVHK YAIGRQVLRT PRA
Validated NT Sequence
aggcgcgcgg agtccgaagc acctggcgtc cgatggcgta cttgtgcacc tcggtgggac cgtcgtacag acggaaggcg cgcatatcac ggaagatcat ctccaccggc gtctcatcgc tgatgccgat gccgccgagc acctgaacac accggtcggt caccttgaac aactcctcgg acacgaagga cttcgccatc gaactttcgt gccggccctt ggcgccgttg tccatcatcc agcaggcatg ccagatggtc agtcgacact gatgcaacgc gatctcgttg tcggcgagca tgaaggaaac cccctggtgc tcgccgatcg tcttaccgaa cgaggtccgg gttcgcgcat agtcgaccgc gatgtcctgg gcccgggagg cggcaccgag ccagcgcata cagtgcgtca accgggccgg ggccagccgc agctgcgcgt atcggaacgc ctgcccggtc tcaccgagca acgcctcccg cggcagggtg agattgtcga accgcaccac ggcatgcccc tcggcgtagt tgcggtccat ggtgttcatg atgcgttcga tcacaatgcc ttcggtctcg ccgtcgcaga gaaacagtgt cggcccctcc ggcaggtgat cgttggcctc caggttggcc atgatgatcc acgttttggc gccgcgagct ccggtgatga gccatttacg gccgttgatg acgaaattct gcccgtcgaa ggtcgccgag gtcttcaact gcgacggatc ggaaccggcc ccgtcgggtt cggtcatcgc gaacgccgag cgctgatacc cctcgatgac gggacgcaga aacttctcgg attgttcctc gttggtgatc ttgccgagca ggaacatgtt gccctcatcg ggtgccgcac agttcatggc gatgggcccc agggtcgacc agcccgcggc ctcgaagatc accgcctggc cgatgtgcga aagaccccac ccaccatagg attctggcgc ctgaacggtc agcagaccgg ccgccctggc cttaccgacc aactcgtcgc gcagctcctc ggtgggtccg tgcgcggtca ggcgaggatc gcgctcgtac ggaatcacgg tctcggtgac gaactcacgc acccgctgcg ccttcgcggc cagctcttcc ggaatgctga agtcgatcat cgaaccagga ccctgggtct gagcttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDFSIPEE LAAKAQRVRE FVTETVIPYE RDPRLTAHGP TEELRDELVG KARAAGLLTV QAPESYGGWG LSHIGQAVIF EAAGWSTLGP IAMNCAAPDE GNMFLLGKIT NEEQSEKFLR PVIEGYQRSA FAMTEPDGAG SDPSQLKTSA TFDGQNFVIN GRKWLITGAR GAKTWIIMAN LEANDHLPEG PTLFLCDGET EGIVIERIMN TMDRNYAEGH AVVRFDNLTL PREALLGETG QAFRYAQLRL APARLTHCMR WLGAASRAQD IAVDYARTRT SFGKTIGEHQ GVSFMLADNE IALHQCRLTI WHACWMMDNG AKGRHESSMA KSFVSEELFK VTDRCVQVLG GIGISDETPV EMIFRDMRAF RLYDGPTEVH KYAIGRQVLR TPRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCGACTTC AGCATTCCGG AAGAGCTGGC CGCGAAGGCG CAGCGGGTGC GTGAGTTCGT CACCGAGACC GTGATTCCGT ACGAGCGCGA TCCTCGCCTG ACCGCGCACG GACCCACCGA GGAGCTGCGC GACGAGTTGG TCGGTAAGGC CAGGGCGGCC GGTCTGCTGA CCGTTCAGGC GCCAGAATCC TATGGTGGGT GGGGTCTTTC GCACATCGGC CAGGCGGTGA TCTTCGAGGC CGCGGGCTGG TCGACCCTGG GGCCCATCGC CATGAACTGT GCGGCACCCG ATGAGGGCAA CATGTTCCTG CTCGGCAAGA TCACCAACGA GGAACAATCC GAGAAGTTTC TGCGTCCCGT CATCGAGGGG TATCAGCGCT CGGCGTTCGC GATGACCGAA CCCGACGGGG CCGGTTCCGA TCCGTCGCAG TTGAAGACCT CGGCGACCTT CGACGGGCAG AATTTCGTCA TCAACGGCCG TAAATGGCTC ATCACCGGAG CTCGCGGCGC CAAAACGTGG ATCATCATGG CCAACCTGGA GGCCAACGAT CACCTGCCGG AGGGGCCGAC ACTGTTTCTC TGCGACGGCG AGACCGAAGG CATTGTGATC GAACGCATCA TGAACACCAT GGACCGCAAC TACGCCGAGG GGCATGCCGT GGTGCGGTTC GACAATCTCA CCCTGCCGCG GGAGGCGTTG CTCGGTGAGA CCGGGCAGGC GTTCCGATAC GCGCAGCTGC GGCTGGCCCC GGCCCGGTTG ACGCACTGTA TGCGCTGGCT CGGTGCCGCC TCCCGGGCCC AGGACATCGC GGTCGACTAT GCGCGAACCC GGACCTCGTT CGGTAAGACG ATCGGCGAGC ACCAGGGGGT TTCCTTCATG CTCGCCGACA ACGAGATCGC GTTGCATCAG TGTCGACTGA CCATCTGGCA TGCCTGCTGG ATGATGGACA ACGGCGCCAA GGGCCGGCAC GAAAGTTCGA TGGCGAAGTC CTTCGTGTCC GAGGAGTTGT TCAAGGTGAC CGACCGGTGT GTTCAGGTGC TCGGCGGCAT CGGCATCAGC GATGAGACGC CGGTGGAGAT GATCTTCCGT GATATGCGCG CCTTCCGTCT GTACGACGGT CCCACCGAGG TGCACAAGTA CGCCATCGGA CGCCAGGTGC TTCGGACTCC GCGCGCCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA