SSGCID: Target Page

MypaA.00309.a

FadE2

CENTER ID:	MypaA.00309.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00309.a.A1.GE29424	Full length( MypaA.00309.a )		1	410

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00309.a.A1.PW32133	Full length( MypaA.00309.a )		1	410

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3800
RefSeq:	NP_962504.1
UniProt:	Q73TZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFAMSAKAS DYHKRLTDFM TEHVFAAEAE YDKYRHEAGP HDHTVPPVVE QLKVKAKQQG LWNLFLPAES GLTNLEYAPL AEITGWSLEI APEALNCAAP DTGNMETLHL FATEEQRKQW LEPLLAGEIR SAFSMTEPAV ASSDARNIET SIVRDGGDYV INGRKWWTSG AADPRCKILI VMGRTNPDAA SHQQQSMVLV PMDTPGVTVV RSTPVFGWQD QHGHCEIVYD NVRVPVTNLL GEEGSGFAIA QARLGPGRIH HCMRALGGAE RALALMVNRA QKRVAFGRPL AEQGVVRESI AKSRNEIDQA RLLCEKAAWT IDQHGNKAAH LLVSQIKAVA PKVACDVIDR AIQVHGAAGV SDDTVLARLY GWHRAMRIFD GPDEVHMRTI ARAELGREKS ALAAAVTAHA

NT Sequence

ATGGACTTCG CGATGTCGGC CAAAGCCAGC GACTACCACA AGCGGCTGAC CGATTTCATG ACCGAGCACG TCTTCGCGGC CGAGGCCGAA TACGACAAGT ACCGGCACGA GGCCGGCCCG CACGACCACA CCGTCCCGCC GGTGGTGGAA CAGCTCAAGG TCAAGGCCAA GCAGCAGGGG CTGTGGAACC TGTTCCTGCC CGCCGAGTCG GGTCTGACCA ACCTGGAATA CGCGCCGCTG GCCGAGATCA CCGGCTGGAG CCTGGAGATC GCGCCCGAGG CGCTCAACTG CGCGGCGCCC GACACCGGCA ACATGGAGAC GCTGCACCTG TTCGCCACCG AGGAGCAGCG CAAGCAGTGG CTGGAGCCGT TGCTGGCCGG CGAGATCCGC AGCGCCTTCT CCATGACCGA GCCCGCCGTC GCGTCCAGCG ACGCCCGCAA CATCGAGACC TCCATCGTGC GCGACGGCGG CGACTACGTG ATCAACGGCC GCAAGTGGTG GACGTCGGGC GCGGCGGACC CGCGCTGCAA GATCCTGATC GTGATGGGCC GCACCAACCC CGACGCGGCC AGCCACCAAC AACAGTCGAT GGTGCTGGTG CCGATGGACA CCCCGGGGGT GACGGTGGTG CGCTCCACCC CGGTGTTCGG CTGGCAGGAC CAGCACGGCC ACTGCGAGAT CGTCTACGAC AACGTGCGGG TGCCGGTCAC CAACCTGCTC GGCGAGGAGG GCTCGGGCTT CGCGATCGCC CAGGCCCGGC TCGGTCCCGG CCGCATCCAC CACTGCATGC GCGCCCTCGG CGGGGCCGAG CGGGCGCTGG CGCTGATGGT CAACCGGGCC CAAAAGCGGG TGGCGTTCGG CCGCCCGCTG GCCGAGCAGG GCGTGGTCCG GGAGTCGATT GCCAAGTCCC GCAACGAAAT CGACCAGGCG CGGCTGCTCT GCGAGAAGGC GGCGTGGACC ATCGACCAGC ACGGCAACAA GGCCGCGCAT CTGCTGGTCT CCCAGATCAA GGCGGTGGCG CCGAAGGTGG CCTGTGACGT CATCGACCGG GCCATCCAGG TGCACGGCGC CGCCGGGGTC AGCGACGACA CGGTGCTGGC CCGGCTGTAC GGCTGGCATC GCGCCATGCG GATCTTCGAC GGCCCGGACG AGGTGCACAT GCGGACCATC GCCCGCGCCG AACTGGGCCG GGAAAAGTCT GCGCTCGCCG CGGCGGTCAC CGCGCATGCC

Details for MypaA.00309.a.A1.GE29424

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	47kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MDFAMSAKAS DYHKRLTDFM TEHVFAAEAE YDKYRHEAGP HDHTVPPVVE QLKVKAKQQG LWNLFLPAES GLTNLEYAPL AEITGWSLEI APEALNCAAP DTGNMETLHL FATEEQRKQW LEPLLAGEIR SAFSMTEPAV ASSDARNIXT SIVRDGGDYV INGRKWWTSG AADPRCKILI VMGRTNPDAA SHQQQSMVLV PMDTPGVTVV RSTPVFGWQD QHGHCEIVYD NVRVPVTNLL GEEGSGFAIA QARLGPGRIH HCMRALGGAE RALALMVNRA QKRVAFGRPL AEQGVVRESI AKSRNEIDQA RLLCEKAAWT IDQHGNKAAH LLVSQIKAVA PKVACDVIDR AIQVHGAAGV SDDTVLARLY GWHRAMRIFD GPDEVHMRTI ARAELGREKS ALAAAVT

Validated NT Sequence

cgcggtgacc gccgcggcga gcgcagactt ttcccggccc agttcggcgc gggcgatggt ccgcatgtgc acctcgtccg ggccgtcgaa gatccgcatg gcgcgatgcc agccgtacag ccgggccagc accgtgtcgt cgctgacccc ggcggcgccg tgcacctgga tggcccggtc gatgacgtca caggccacct tcggcgccac cgccttgatc tgggagacca gcagatgcgc ggccttgttg ccgtgctggt cgatggtcca cgccgccttc tcgcagagca gccgcgcctg gtcgatttcg ttgcgggact tggcaatcga ctcccggacc acgccctgct cggccagcgg gcggccgaac gccacccgct tttgggcccg gttgaccatc agcgccagcg cccgctcggc cccgccgagg gcgcgcatgc agtggtggat gcggccggga ccgagccggg cctgggcgat cgcgaagccc gagccctcct cgccgagcag gttggtgacc ggcacccgca cgttgtcgta gacgatctcg cagtggccgt gctggtcctg ccagccgaac accggggtgg agcgcaccac cgtcaccccc ggggtgtcca tcggcaccag caccatcgac tgttgttggt ggctggccgc gtcggggttg gtgcggccca tcacgatcag gatcttgcag cgcgggtccg ccgcgcccga cgtccaccac ttgcggccgt tgatcacgta gtcgccgccg tcgcgcacga tggaggtntc gatgttgcgg gcgtcgctgg acgcgacggc gggctcggtc atggagaagg cgctgcggat ctcnccggcc agcaacggct ccagccactg cttgcgctgc tcctcggtgg cgaacaggtg cagcgtctcc atgttgccgg tgtcgggcgc cgcgcagttg agcgcctcgg gcgcgatctc caggctccag ccggtgatct cggccagcgg cgcgtattcc aggttggtca gacccgactc ggcgggcagg aacaggttcc acagcccctg ctgcttggcc ttgaccttga gctgttccac caccggcggg acggtgtggt cgtgcgggcc ggcctcgtgc cggtacttgt cgtattcggc ctcggccgcg aagacgtgct cggtcatgaa atcggtcagc cgcttgtggt agtcgctggc tttggccgac atcgcgaagt ccatcgaacc aggaccctgg gtctgagctt ccagggtacc ca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDFAMSAKA SDYHKRLTDF MTEHVFAAEA EYDKYRHEAG PHDHTVPPVV EQLKVKAKQQ GLWNLFLPAE SGLTNLEYAP LAEITGWSLE IAPEALNCAA PDTGNMETLH LFATEEQRKQ WLEPLLAGEI RSAFSMTEPA VASSDARNIE TSIVRDGGDY VINGRKWWTS GAADPRCKIL IVMGRTNPDA ASHQQQSMVL VPMDTPGVTV VRSTPVFGWQ DQHGHCEIVY DNVRVPVTNL LGEEGSGFAI AQARLGPGRI HHCMRALGGA ERALALMVNR AQKRVAFGRP LAEQGVVRES IAKSRNEIDQ ARLLCEKAAW TIDQHGNKAA HLLVSQIKAV APKVACDVID RAIQVHGAAG VSDDTVLARL YGWHRAMRIF DGPDEVHMRT IARAELGREK SALAAAVTAH A

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacttcgcg atgtcggcca aagccagcga ctaccacaag cggctgaccg atttcatgac cgagcacgtc ttcgcggccg aggccgaata cgacaagtac cggcacgagg ccggcccgca cgaccacacc gtcccgccgg tggtggaaca gctcaaggtc aaggccaagc agcaggggct gtggaacctg ttcctgcccg ccgagtcggg tctgaccaac ctggaatacg cgccgctggc cgagatcacc ggctggagcc tggagatcgc gcccgaggcg ctcaactgcg cggcgcccga caccggcaac atggagacgc tgcacctgtt cgccaccgag gagcagcgca agcagtggct ggagccgttg ctggccggcg agatccgcag cgccttctcc atgaccgagc ccgccgtcgc gtccagcgac gcccgcaaca tcgagacctc catcgtgcgc gacggcggcg actacgtgat caacggccgc aagtggtgga cgtcgggcgc ggcggacccg cgctgcaaga tcctgatcgt gatgggccgc accaaccccg acgcggccag ccaccaacaa cagtcgatgg tgctggtgcc gatggacacc ccgggggtga cggtggtgcg ctccaccccg gtgttcggct ggcaggacca gcacggccac tgcgagatcg tctacgacaa cgtgcgggtg ccggtcacca acctgctcgg cgaggagggc tcgggcttcg cgatcgccca ggcccggctc ggtcccggcc gcatccacca ctgcatgcgc gccctcggcg gggccgagcg ggcgctggcg ctgatggtca accgggccca aaagcgggtg gcgttcggcc gcccgctggc cgagcagggc gtggtccggg agtcgattgc caagtcccgc aacgaaatcg accaggcgcg gctgctctgc gagaaggcgg cgtggaccat cgaccagcac ggcaacaagg ccgcgcatct gctggtctcc cagatcaagg cggtggcgcc gaaggtggcc tgtgacgtca tcgaccgggc catccaggtg cacggcgccg ccggggtcag cgacgacacg gtgctggccc ggctgtacgg ctggcatcgc gccatgcgga tcttcgacgg cccggacgag gtgcacatgc ggaccatcgc ccgcgccgaa ctgggccggg aaaagtctgc gctcgccgcg gcggtcaccg cgcatgccaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MypaA.00309.a.A1.PW32133

PURIFICATION DATe:	4/15/2011
CONCENTRATION:	35.05mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACTTCGCG ATGTCGGCCA AAGCCAGCGA CTACCACAAG CGGCTGACCG ATTTCATGAC CGAGCACGTC TTCGCGGCCG AGGCCGAATA CGACAAGTAC CGGCACGAGG CCGGCCCGCA CGACCACACC GTCCCGCCGG TGGTGGAACA GCTCAAGGTC AAGGCCAAGC AGCAGGGGCT GTGGAACCTG TTCCTGCCCG CCGAGTCGGG TCTGACCAAC CTGGAATACG CGCCGCTGGC CGAGATCACC GGCTGGAGCC TGGAGATCGC GCCCGAGGCG CTCAACTGCG CGGCGCCCGA CACCGGCAAC ATGGAGACGC TGCACCTGTT CGCCACCGAG GAGCAGCGCA AGCAGTGGCT GGAGCCGTTG CTGGCCGGCG AGATCCGCAG CGCCTTCTCC ATGACCGAGC CCGCCGTCGC GTCCAGCGAC GCCCGCAACA TCGAGACCTC CATCGTGCGC GACGGCGGCG ACTACGTGAT CAACGGCCGC AAGTGGTGGA CGTCGGGCGC GGCGGACCCG CGCTGCAAGA TCCTGATCGT GATGGGCCGC ACCAACCCCG ACGCGGCCAG CCACCAACAA CAGTCGATGG TGCTGGTGCC GATGGACACC CCGGGGGTGA CGGTGGTGCG CTCCACCCCG GTGTTCGGCT GGCAGGACCA GCACGGCCAC TGCGAGATCG TCTACGACAA CGTGCGGGTG CCGGTCACCA ACCTGCTCGG CGAGGAGGGC TCGGGCTTCG CGATCGCCCA GGCCCGGCTC GGTCCCGGCC GCATCCACCA CTGCATGCGC GCCCTCGGCG GGGCCGAGCG GGCGCTGGCG CTGATGGTCA ACCGGGCCCA AAAGCGGGTG GCGTTCGGCC GCCCGCTGGC CGAGCAGGGC GTGGTCCGGG AGTCGATTGC CAAGTCCCGC AACGAAATCG ACCAGGCGCG GCTGCTCTGC GAGAAGGCGG CGTGGACCAT CGACCAGCAC GGCAACAAGG CCGCGCATCT GCTGGTCTCC CAGATCAAGG CGGTGGCGCC GAAGGTGGCC TGTGACGTCA TCGACCGGGC CATCCAGGTG CACGGCGCCG CCGGGGTCAG CGACGACACG GTGCTGGCCC GGCTGTACGG CTGGCATCGC GCCATGCGGA TCTTCGACGG CCCGGACGAG GTGCACATGC GGACCATCGC CCGCGCCGAA CTGGGCCGGG AAAAGTCTGC GCTCGCCGCG GCGGTCACCG CGCATGCCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA