MytuD.01609.a

POSSIBLE OSMOPROTECTANT (GLYCINE BETAINE/CARNITINE/CHOLINE/L-PROLINE) TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER PROV

CENTER ID: MytuD.01609.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01609.a.B1.GE26991 Full length( MytuD.01609.a ) 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4187
RefSeq: NP_218275.1
TubercuList: Rv3758c
MTB Network Portal: Rv3758c
UniProt: O69724

Orthologues for Rv3758c

Species % Identity % Coverage Clones Proteins Structures
marinum 79 96 1 0 0
paratuberculosis 79 73 1 0 0
avium 78 96 1 0 0
fortuitum 76 38 0 0 0
smegmatis 67 95 0 0 0
abscessus 66 95 1 0 0
abscessus 49 79 1 0 0
marinum 48 78 1 0 0
smegmatis 38 55 0 0 0
smegmatis 32 54 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MICFDDVSKV YAHGATAVDR LTLEVPNGML TVFVGPSGCG KTTALRMINR MVDPTSGTIT VDGTDVSTVN AVKLRLGIGY VIQNAGLMPH QRVIDNVATV PVLKGQPRRA ARKAGYEVLE RVGLDPKVAT RYPAQLSGGE QQRVGVARAL AADPPILLMD EPFSAVDPVV RHELQNEILR LQAELHKTIV FVTHDIDEAL KLADLVAVFA PGGALAQYDE TARLLSSPAN DFVSKFIGLG RGYRWLQLFD AAGLPVRDIE QVSVNGLSDA RDRQVRDGWV LVVDGAGAPL GWIDADGRRR HRGGAALSDA MTVGGSVFRP NGNLSQALDA ALSSPSGVGV AVDGGGKVIG GILAADVLAE FQKGKKAGGG AKPCTT
NT Sequence
TTGATCTGCT TTGACGATGT CAGCAAGGTG TACGCACACG GTGCCACCGC CGTAGACCGG CTGACGCTGG AAGTCCCTAA CGGCATGCTG ACCGTCTTCG TCGGCCCCTC CGGCTGCGGC AAGACGACGG CGCTGCGAAT GATCAACCGA ATGGTGGATC CGACCTCGGG CACCATCACT GTCGACGGTA CCGACGTGTC GACGGTCAAT GCGGTGAAGC TGCGCCTGGG AATTGGCTAT GTCATCCAGA ACGCGGGGCT GATGCCTCAT CAACGGGTCA TCGACAACGT CGCAACGGTG CCGGTGCTGA AGGGTCAGCC GCGCCGGGCA GCCCGCAAAG CCGGTTATGA GGTGCTTGAG CGTGTCGGGC TGGACCCCAA GGTCGCCACC CGCTACCCGG CCCAGCTCTC GGGCGGCGAA CAGCAACGGG TCGGCGTGGC ACGGGCACTC GCGGCCGATC CGCCGATCTT GTTGATGGAC GAGCCGTTCT CGGCCGTCGA CCCGGTGGTT CGCCACGAGC TACAGAACGA AATACTTCGT CTGCAAGCCG AGTTGCACAA GACCATTGTC TTCGTGACGC ACGACATCGA CGAGGCGTTG AAGCTCGCCG ATCTGGTGGC GGTGTTCGCC CCGGGCGGCG CGCTTGCGCA GTACGACGAA ACTGCCCGGC TGTTATCCAG TCCGGCGAAT GACTTCGTGT CGAAGTTCAT CGGTCTCGGT CGCGGCTATC GGTGGCTGCA GCTGTTCGAC GCGGCCGGAC TACCTGTGCG CGACATCGAG CAAGTCTCGG TGAACGGCCT TTCCGATGCC CGGGACAGGC AAGTTCGTGA CGGCTGGGTG CTGGTGGTCG ACGGTGCGGG TGCGCCGTTG GGCTGGATCG ACGCCGATGG CCGGCGGCGT CACCGCGGCG GCGCGGCATT GTCGGATGCC ATGACCGTCG GCGGTTCGGT GTTCCGCCCG AACGGTAACC TCAGCCAGGC GCTGGACGCC GCCTTGTCCT CGCCGTCGGG GGTCGGTGTC GCCGTTGACG GCGGTGGCAA GGTCATCGGC GGGATACTGG CCGCCGACGT GCTGGCCGAG TTCCAAAAAG GCAAGAAGGC CGGCGGCGGA GCTAAGCCAT GCACTACC
Details for MytuD.01609.a.B1.GE26991
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/15/2009
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML ICFDDVSKVY AHGATAVDRL TLEVPNGMLT VFVGPSGCGK TTALRMINRM VDPTSGTITV DGTDVSTVNA VKLRLGIGYV IQNAGLMPHQ RVIDNVATVP VLKGQPRRAA RKAGYEVLER VGLDPKVATR YPAQLSGGEQ QRVGVARALA ADPPILLMDE PFSAVDPVVR HELQNEILRL QAELHKTIVF VTHDIDEALK LADLVAVFAP GGALAQYDET ARLLSSPAND FVSKFIGLGR GYRWLQLFDA AGLPVRDIEQ VSVNGLSDAR DRQVRDGWVL VVDGAGAPLG WIDADGRRRX RGGAALSDAM TVGGXVFRPN GNLSQALDXA LSXPSGVGVA VXGGGKVIGG XLAADXLAEF QKGKKAGG
Validated NT Sequence
tttaagaagg agntatacca tggctcacca ccaccaccac catatgttga tctgctttga cgatgtcagc aaggtgtacg cacacggtgc caccgccgta gaccggctga cgctggaagt ccctaacggc atgctgaccg tcttcgtcgg cccctccggc tgcggcaaga cgacggcgct gcgaatgatc aaccgaatgg tggatccgac ctcgggcacc atcactgtcg acggtaccga cgtgtcgacg gtcaatgcgg tgaagctgcg cctgggaatt ggctatgtca tccagaacgc ggggctgatg cctcatcaac gggtcatcga caacgtcgca acggtgccgg tgctgaaggg tcagccgcgc cgggcagccc gcaaagccgg ttatgaggtg cttgagcgtg tcgggctgga ccccaaggtc gccacccgct acccggccca gctctcgggc ggcgaacagc aacgggtcgg cgtggcacgg gcactcgcgg ccgatccgcc gatcttgttg atggacgagc cgttctcggc cgtcgacccg gtggttcgcc acgagctaca gaacgaaata cttcgtctgc aagccgagtt gcacaagacc attgtcttcg tgacgcacga catcgacgag gcgttgaagc tcgccgatct ggtggcggtg ttcgccccgg gcggcgcgct tgcgcagtac gacgaaactg cccggctgtt atccagtccg gcgaatgact tcgtgtcgaa gttcatcggt ctcggtcgcg gctatcggtg gctgcagctg ttcgacgcgg ccggactacc tgtgcgcgac atcgagcaag tctcggtgaa cggcctttcc gatgcccggg acaggcaagt tcgtgacggc tgggtgctgg tggtcgacgg tgcgggtgcg ccgttgggct ggatcgacgc cgatggccgg cggcgtcncc gcggcggcgc ggcattgtcg gatgccatga ccgtcggcgg ttnggtgttc cgcccgaacg gtaacctcag ccaggcgctg gacnccgcct tgtccnngcc gtcgggggtc ggtgtcgccg ttgncggcgg nggcaaggtn atcggcggga tnctggccgc ngacgngctg gccgagttcc aaaaaggcaa gaaggccggc ggcgga
Expected Protein Sequence
MAHHHHHHML ICFDDVSKVY AHGATAVDRL TLEVPNGMLT VFVGPSGCGK TTALRMINRM VDPTSGTITV DGTDVSTVNA VKLRLGIGYV IQNAGLMPHQ RVIDNVATVP VLKGQPRRAA RKAGYEVLER VGLDPKVATR YPAQLSGGEQ QRVGVARALA ADPPILLMDE PFSAVDPVVR HELQNEILRL QAELHKTIVF VTHDIDEALK LADLVAVFAP GGALAQYDET ARLLSSPAND FVSKFIGLGR GYRWLQLFDA AGLPVRDIEQ VSVNGLSDAR DRQVRDGWVL VVDGAGAPLG WIDADGRRRH RGGAALSDAM TVGGSVFRPN GNLSQALDAA LSSPSGVGVA VDGGGKVIGG ILAADVLAEF QKGKKAGGGA KPCTT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga tctgctttga cgatgtcagc aaggtgtacg cacacggtgc caccgccgta gaccggctga cgctggaagt ccctaacggc atgctgaccg tcttcgtcgg cccctccggc tgcggcaaga cgacggcgct gcgaatgatc aaccgaatgg tggatccgac ctcgggcacc atcactgtcg acggtaccga cgtgtcgacg gtcaatgcgg tgaagctgcg cctgggaatt ggctatgtca tccagaacgc ggggctgatg cctcatcaac gggtcatcga caacgtcgca acggtgccgg tgctgaaggg tcagccgcgc cgggcagccc gcaaagccgg ttatgaggtg cttgagcgtg tcgggctgga ccccaaggtc gccacccgct acccggccca gctctcgggc ggcgaacagc aacgggtcgg cgtggcacgg gcactcgcgg ccgatccgcc gatcttgttg atggacgagc cgttctcggc cgtcgacccg gtggttcgcc acgagctaca gaacgaaata cttcgtctgc aagccgagtt gcacaagacc attgtcttcg tgacgcacga catcgacgag gcgttgaagc tcgccgatct ggtggcggtg ttcgccccgg gcggcgcgct tgcgcagtac gacgaaactg cccggctgtt atccagtccg gcgaatgact tcgtgtcgaa gttcatcggt ctcggtcgcg gctatcggtg gctgcagctg ttcgacgcgg ccggactacc tgtgcgcgac atcgagcaag tctcggtgaa cggcctttcc gatgcccggg acaggcaagt tcgtgacggc tgggtgctgg tggtcgacgg tgcgggtgcg ccgttgggct ggatcgacgc cgatggccgg cggcgtcacc gcggcggcgc ggcattgtcg gatgccatga ccgtcggcgg ttcggtgttc cgcccgaacg gtaacctcag ccaggcgctg gacgccgcct tgtcctcgcc gtcgggggtc ggtgtcgccg ttgacggcgg tggcaaggtc atcggcggga tactggccgc cgacgtgctg gccgagttcc aaaaaggcaa gaaggccggc ggcggagcta agccatgcac tacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat