MymaA.01609.b

Osmoprotectant transport ATP-binding protein ABC transporter ProV

CENTER ID: MymaA.01609.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01609.b.A1.GE30215 Full length( MymaA.01609.b ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5685
RefSeq: YP_001853560.1
UniProt: B2HLH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRFDNVDKV YADGTTAVDQ LSLEVADGML TVFVGPSGCG KTTALRMINR MVEPSSGTVT VNGTDVSTVN PVKLRLGIGY VIQNAGLMPH QRVIDNVATV PVLKGQSRRA ARKAAYEVLE RVGLNPKVAS RYPAQLSGGE QQRVGVARAL AADPPILLMD EAFSAVDPVV RHDLQNEILR LQSDLHKTIV FVTHDIDEAF KLGEVVAVFA PGGRLQQCDQ PARLLARPAN EFVSKFIGLG RGYRLLQLFD GAGLELHDLE RICVSSLGRS QLPDGWALVV SDDDVPVGWI DADGLRRHRD GATLPDAMTV VGAVFRPNGN LSQALDAALS SPSSMGVAVD DGGKVIGGVL ADDVLSALKS RR
NT Sequence
TTGATCAGGT TTGACAATGT CGACAAGGTG TACGCCGACG GCACCACCGC CGTGGACCAA CTGAGCCTCG AAGTCGCCGA CGGCATGCTG ACCGTTTTCG TCGGCCCATC GGGCTGTGGC AAGACGACCG CGCTGCGCAT GATCAACCGC ATGGTCGAGC CGAGTTCGGG CACCGTCACC GTCAACGGCA CCGACGTCTC CACGGTGAAC CCGGTGAAGT TGCGCCTCGG GATCGGCTAC GTCATCCAGA ACGCGGGGCT GATGCCCCAT CAGCGGGTGA TCGACAATGT CGCCACGGTG CCGGTGCTGA AGGGGCAGTC CCGTCGGGCC GCCCGCAAAG CCGCCTACGA AGTACTCGAA CGGGTAGGTC TGAACCCCAA GGTGGCCAGC CGTTATCCGG CCCAACTCTC CGGTGGCGAA CAACAGCGGG TCGGGGTGGC GCGGGCGCTG GCGGCTGACC CGCCGATCCT GTTGATGGAC GAGGCCTTCT CTGCCGTCGA CCCGGTGGTA CGCCATGACC TGCAGAACGA AATCCTGCGT CTGCAAAGCG ATCTACACAA GACCATCGTT TTCGTCACGC ACGACATCGA CGAGGCGTTC AAACTCGGCG AGGTGGTGGC GGTGTTCGCC CCGGGCGGTC GGTTGCAGCA ATGTGACCAG CCCGCCCGGC TGCTGGCCCG CCCGGCCAAT GAGTTCGTGT CGAAATTTAT TGGCTTGGGT CGTGGCTACC GGTTGCTGCA ACTCTTCGAT GGGGCGGGGC TCGAGCTGCA TGACCTCGAG CGGATCTGTG TGAGCAGTCT GGGGCGCTCG CAACTGCCCG ATGGCTGGGC TCTGGTGGTC AGCGACGATG ACGTCCCGGT GGGCTGGATT GACGCCGACG GATTGCGCCG CCACCGCGAT GGTGCGACCT TGCCGGACGC CATGACCGTG GTCGGGGCGG TATTCCGGCC GAACGGCAAC CTTAGCCAGG CGCTGGATGC CGCGCTGTCG TCGCCGTCAT CCATGGGTGT GGCCGTCGAC GACGGCGGCA AGGTCATCGG TGGAGTGCTG GCAGATGACG TGCTGTCCGC ATTGAAAAGC CGGCGA
Details for MymaA.01609.b.A1.GE30215
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLIRFDNVD KVYADGTTAV DQLSLEVADG MLTVFVGPSG CGKTTALRMI NRMVEPXSGT VTVNGTDVST VNPVKLRLGI GYVIQNAGLM PHQRVIDNVA TVXXLKGQSR RAARKAAYEV LERVGLNPKV ASRYPAQLSG GEQQRVGVAR ALAADPPILL MDEAFSAVDP VVRHDLQNEI LRLQSDLHKT IVFVTHDIDE AFKLGEVVAV FAPGGRLQQC XQPARLLXRP XNEFVSKFIG LGRGYRLLXL FXGAGLELHD LXRICVSSLG RSQLPDGWAL XVSDDDVXWX GLTPXXCXXX MVRXXXXXXX GXXXXXXXXX XXXXXXXXGX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
tannnnngtt tactttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgtt gatcaggttt gacaatgtcg acaaggtgta cgccgacggc accaccgccg tggaccaact gagcctcgaa gtcgccgacg gcatgctgac cgttttcgtc ggcccatcgg gctgtggcaa gacgaccgcg ctgcgcatga tcaaccgcat ggtcgagccg agntcnggca ccgtcaccgt caacggcacc gacgtctcca cggtgaaccc ggtgaagttg cgcctcggga tcggctacgt catccagaac gcggggctga tgccccatca gcgggtgatc gacaatgtcg ccacggtgnn ngngctgaag gggcagtccc gtcgggccgc ccgcaaagcc gcctacgaag tactcgaacg ggtaggtctg aaccccaagg tggccagccg ttatccggcc caactctccg gtggcgaaca acagcgggtc ggggtggcgc gggcgctggc ggctgacccg ccgatcctgt tgatggacga ggccttctct gccgtcgacc cggtggtacg ccatgacctg cagaacgaaa tcctgcgtct gcaaagcgat ctacacaaga ccatcgtttt cgtcacgcac gacatcgacg aggcgttcaa actcggcgag gtggtggcgg tgttcgcccc gggcggtcgg ttgcagcaat gtgancagcc cgcccggctg ctggnccgcc cggncaatga gttcgtgtcg aaatttattg gcttgggtcg tggctaccgg ttgctgcnnc tcttcnatgg ggcggggctc gagctgcatg acctcnagcg gatctgtgtg agcagtctgg ggcgctcgca actgcccgat ggctgggctc tnntggtcag cgacgatgac gtcnngtggg ntggnttgac gccnangnat tgcnnncncn nnatggtgcg acntnnnnnn nnnnncnngn ngggngnnnt nnnnangnan nnnnngnnnn gnnncnnnnt nncnnnnnnt cntcntggnn nnnnnnnacn nnnnnnnnnn nntnnnnnnn nnntncncnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngnanncn nnnnnnnann annnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLIRFDNVD KVYADGTTAV DQLSLEVADG MLTVFVGPSG CGKTTALRMI NRMVEPSSGT VTVNGTDVST VNPVKLRLGI GYVIQNAGLM PHQRVIDNVA TVPVLKGQSR RAARKAAYEV LERVGLNPKV ASRYPAQLSG GEQQRVGVAR ALAADPPILL MDEAFSAVDP VVRHDLQNEI LRLQSDLHKT IVFVTHDIDE AFKLGEVVAV FAPGGRLQQC DQPARLLARP ANEFVSKFIG LGRGYRLLQL FDGAGLELHD LERICVSSLG RSQLPDGWAL VVSDDDVPVG WIDADGLRRH RDGATLPDAM TVVGAVFRPN GNLSQALDAA LSSPSSMGVA VDDGGKVIGG VLADDVLSAL KSRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgatcagg tttgacaatg tcgacaaggt gtacgccgac ggcaccaccg ccgtggacca actgagcctc gaagtcgccg acggcatgct gaccgttttc gtcggcccat cgggctgtgg caagacgacc gcgctgcgca tgatcaaccg catggtcgag ccgagttcgg gcaccgtcac cgtcaacggc accgacgtct ccacggtgaa cccggtgaag ttgcgcctcg ggatcggcta cgtcatccag aacgcggggc tgatgcccca tcagcgggtg atcgacaatg tcgccacggt gccggtgctg aaggggcagt cccgtcgggc cgcccgcaaa gccgcctacg aagtactcga acgggtaggt ctgaacccca aggtggccag ccgttatccg gcccaactct ccggtggcga acaacagcgg gtcggggtgg cgcgggcgct ggcggctgac ccgccgatcc tgttgatgga cgaggccttc tctgccgtcg acccggtggt acgccatgac ctgcagaacg aaatcctgcg tctgcaaagc gatctacaca agaccatcgt tttcgtcacg cacgacatcg acgaggcgtt caaactcggc gaggtggtgg cggtgttcgc cccgggcggt cggttgcagc aatgtgacca gcccgcccgg ctgctggccc gcccggccaa tgagttcgtg tcgaaattta ttggcttggg tcgtggctac cggttgctgc aactcttcga tggggcgggg ctcgagctgc atgacctcga gcggatctgt gtgagcagtc tggggcgctc gcaactgccc gatggctggg ctctggtggt cagcgacgat gacgtcccgg tgggctggat tgacgccgac ggattgcgcc gccaccgcga tggtgcgacc ttgccggacg ccatgaccgt ggtcggggcg gtattccggc cgaacggcaa ccttagccag gcgctggatg ccgcgctgtc gtcgccgtca tccatgggtg tggccgtcga cgacggcggc aaggtcatcg gtggagtgct ggcagatgac gtgctgtccg cattgaaaag ccggcgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa