MymaA.01609.a

Osmoprotectant (Glycine betaine/carnitine/choline/L-proline) transport ATP-binding protein ABC transporter ProV_1

CENTER ID: MymaA.01609.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01609.a.A1.GE30214 Full length( MymaA.01609.a ) 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.65
RefSeq: YP_001848360.1
UniProt: B2HI79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAASADQP TTSDAPQSSG LRILLDGVTK RYGVRSTPAV DNVTLEIGAG EIVMLVGPSG CGKTTTMKMI NRLIEPTSGR IFIGDDDVTR RNPDELRRHI GYVVQGAGLF PHLTVGDNIG IVPRLLKWDK NRVAARIDEL LDLVNLDPAQ YRDRFPRELS GGQQQRVGVA RALAADPPVL LMDEPFGAVD PITRQRLQDE LLRLQEELHK TIVFVTHDFG EAVKLGDRIA ILTTGSKVVQ YDTPERILAE PANDFVRGFV GAGATLMQLS LTRVRDIDLH EAVVAQAGGD PAEAVELAGS LDREHAIVLD RQNRPLRWLP LEELAKPQAL AQVTRDDDLE CVNLASTLND ALDEMLTSSH GVVVVTGRRN TYQGVIRVET IMEAIADIRG AANSEEPDT
NT Sequence
GTGCCTGAAG CCGCATCCGC TGATCAACCG ACCACGTCCG ACGCTCCCCA GAGCAGCGGA CTCCGGATTC TGCTGGATGG GGTGACCAAG CGGTACGGCG TGCGCTCCAC ACCCGCGGTC GACAACGTCA CCTTGGAGAT CGGCGCGGGC GAGATCGTCA TGTTGGTAGG CCCTTCGGGT TGTGGAAAGA CCACGACGAT GAAGATGATC AACCGGCTGA TCGAGCCCAC CAGCGGGCGG ATCTTCATCG GCGATGACGA CGTCACCCGC CGCAACCCCG ATGAACTTCG ACGCCACATC GGTTACGTGG TCCAGGGCGC GGGACTGTTC CCGCACCTGA CGGTGGGTGA CAACATCGGC ATCGTTCCCC GCCTACTCAA GTGGGACAAG AACCGGGTTG CCGCCCGTAT CGACGAACTG CTCGACCTGG TCAACCTCGA CCCGGCCCAG TACCGCGACC GGTTCCCCCG CGAACTCTCC GGGGGCCAGC AGCAGCGCGT CGGTGTCGCT CGCGCCCTGG CCGCCGACCC GCCGGTGCTG TTGATGGACG AGCCGTTCGG TGCGGTCGAC CCGATCACCC GCCAACGACT ACAGGACGAG TTGTTGCGGC TGCAGGAAGA GCTGCACAAG ACCATCGTCT TCGTCACCCA CGACTTCGGC GAGGCCGTCA AGCTGGGTGA CCGGATCGCC ATCCTGACCA CCGGCTCCAA GGTCGTCCAA TACGACACCC CGGAACGGAT CCTCGCCGAA CCCGCCAACG ACTTCGTCCG CGGGTTCGTC GGCGCCGGCG CAACCCTCAT GCAGCTCAGC CTGACCCGGG TGCGCGACAT CGACCTGCAC GAGGCCGTCG TCGCGCAGGC CGGCGGCGAC CCCGCCGAAG CCGTTGAGCT GGCCGGATCC CTCGACCGGG AGCACGCCAT CGTGCTTGAC CGGCAGAATC GGCCGCTGCG CTGGCTGCCC CTCGAGGAGC TGGCCAAACC CCAAGCGCTA GCACAGGTGA CCCGCGATGA CGACCTGGAG TGCGTCAACC TAGCATCGAC CCTCAACGAC GCGTTAGACG AAATGCTGAC CTCGTCGCAC GGGGTCGTGG TCGTCACCGG CCGGCGCAAT ACCTACCAGG GCGTCATTAG AGTCGAGACG ATCATGGAGG CAATCGCCGA CATACGCGGT GCAGCGAACT CTGAGGAGCC CGACACG
Details for MymaA.01609.a.A1.GE30214
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 45kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 78
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPEAASAD QXXXXDAPQS SGLRILLDGV TKRYGVRSTP AVDNVTLEIG AGEIVMLVGP SGCGKTTTMK MINRLIEPTS GRIFIGDDDV TRRNPDELRR HIGYVVQGAG LFPHLTVGDN IGIVPRLLKW DKNRVAARID ELLDLVNLDP AQYRDRFPRE LSGGQQQRVG VARALAADPP VLLMDEPFGA VDPITRQRLQ DELLRLQEEL HKTIVFVTHD FGEAVKLGDR IAILTTGSKV VQYDTPERIL AEPANDFVRG FVGAGATLMQ LXLXRVRDID LHXAVVXXXA ATPPXPLXWX XPSTXXTPSX XXXRXALXXC PXXXXXPXXS XXXXXTTXXX XXXXXPXXXX XXXXXXXXGX XXXXXXXX
Validated NT Sequence
ttntttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgcct gaagccgcat ccgctgatca acannnnnca tncgacgctc cccagagcag cggactccgg attctgctgg atggggtgac caagcggtac ggcgtgcgct ccacacccgc ggtcgacaac gtcaccttgg agatcggcgc gggcgagatc gtcatgttgg taggcccttc gggttgtgga aagaccacga cgatgaagat gatcaaccgg ctgatcgagc ccaccagcgg gcggatcttc atcggcgatg acgacgtcac ccgccgcaac cccgatgaac ttcgacgcca catcggttac gtggtccagg gcgcgggact gttcccgcac ctgacggtgg gtgacaacat cggcatcgtt ccccgcctac tcaagtggga caagaaccgg gttgccgccc gtatcgacga actgctcgac ctggtcaacc tcgacccggc ccagtaccgc gaccggttcc cccgcgaact ctccgggggc cagcagcagc gcgtcggtgt cgctcgcgcc ctggccgccg acccgccggt gctgttgatg gacgagccgt tcggtgcggt cgacccgatc acccgccaac gactacagga cgagttgttg cggctgcagg aagagctgca caagaccatc gtcttcgtca cccacgactt cggcgaggcc gtcaagctgg gtgaccggat cgccatcctg accaccggct ccaaggtcgt ccaatacgac accccggaac ggatcctcgc cgaacccgcc aacgacttcg tccgcgggtt cgtcggcgcc ggngcaaccc tcatgcagct cancctganc cgggtgcgcg acatcgacct gcacnaggcc gtcgtcncgc nnncggcggc gaccccgccg aanccgttga nctggnngna tccctcgacc nggnncacgc catcnngnnt gancngnaga nnggcnctgc nctgntgccc nnnnannnct nnnanccccn ncnntagcnn gnnnncnnnn nnnacnacnn nnnnnnnnnn ncnnancnnc ganccnnann nnnnnnnann nnnnntgann tnnnnnnnnn nnggnntcnt nnannnnnnn nnnnnnatnn nnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPEAASAD QPTTSDAPQS SGLRILLDGV TKRYGVRSTP AVDNVTLEIG AGEIVMLVGP SGCGKTTTMK MINRLIEPTS GRIFIGDDDV TRRNPDELRR HIGYVVQGAG LFPHLTVGDN IGIVPRLLKW DKNRVAARID ELLDLVNLDP AQYRDRFPRE LSGGQQQRVG VARALAADPP VLLMDEPFGA VDPITRQRLQ DELLRLQEEL HKTIVFVTHD FGEAVKLGDR IAILTTGSKV VQYDTPERIL AEPANDFVRG FVGAGATLMQ LSLTRVRDID LHEAVVAQAG GDPAEAVELA GSLDREHAIV LDRQNRPLRW LPLEELAKPQ ALAQVTRDDD LECVNLASTL NDALDEMLTS SHGVVVVTGR RNTYQGVIRV ETIMEAIADI RGAANSEEPD T
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcctgaa gccgcatccg ctgatcaacc gaccacgtcc gacgctcccc agagcagcgg actccggatt ctgctggatg gggtgaccaa gcggtacggc gtgcgctcca cacccgcggt cgacaacgtc accttggaga tcggcgcggg cgagatcgtc atgttggtag gcccttcggg ttgtggaaag accacgacga tgaagatgat caaccggctg atcgagccca ccagcgggcg gatcttcatc ggcgatgacg acgtcacccg ccgcaacccc gatgaacttc gacgccacat cggttacgtg gtccagggcg cgggactgtt cccgcacctg acggtgggtg acaacatcgg catcgttccc cgcctactca agtgggacaa gaaccgggtt gccgcccgta tcgacgaact gctcgacctg gtcaacctcg acccggccca gtaccgcgac cggttccccc gcgaactctc cgggggccag cagcagcgcg tcggtgtcgc tcgcgccctg gccgccgacc cgccggtgct gttgatggac gagccgttcg gtgcggtcga cccgatcacc cgccaacgac tacaggacga gttgttgcgg ctgcaggaag agctgcacaa gaccatcgtc ttcgtcaccc acgacttcgg cgaggccgtc aagctgggtg accggatcgc catcctgacc accggctcca aggtcgtcca atacgacacc ccggaacgga tcctcgccga acccgccaac gacttcgtcc gcgggttcgt cggcgccggc gcaaccctca tgcagctcag cctgacccgg gtgcgcgaca tcgacctgca cgaggccgtc gtcgcgcagg ccggcggcga ccccgccgaa gccgttgagc tggccggatc cctcgaccgg gagcacgcca tcgtgcttga ccggcagaat cggccgctgc gctggctgcc cctcgaggag ctggccaaac cccaagcgct agcacaggtg acccgcgatg acgacctgga gtgcgtcaac ctagcatcga ccctcaacga cgcgttagac gaaatgctga cctcgtcgca cggggtcgtg gtcgtcaccg gccggcgcaa tacctaccag ggcgtcatta gagtcgagac gatcatggag gcaatcgccg acatacgcgg tgcagcgaac tctgaggagc ccgacacgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa