SSGCID: Target Page

MypaA.01609.a

Putative uncharacterized protein

CENTER ID:	MypaA.01609.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01609.a.A1.GE29620	Full length( MypaA.01609.a )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.289
RefSeq:	NP_959207.1
UniProt:	Q745R5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPHQRVIDNV ATVPVLRGQR RRAARTAAYQ VLERVGLDVK LARRYPAQLS GGEQQRVGVA RALAADPPIL LMDEPFSAVD PVVRHDLQNE ILRLQSELHK TIVFVTHDID EALRLGERVA VFKGGVLQQC DEPARLLSRP ANDFVSRFIG LGRGYRWLQL IDAAGLPVHD VPRLSAENLS GADVFDGWAV VVDEAGAPLG WIDAEGLRRH RDGLALSDSM TGVGSLFRPR GNLSQALDAA LSSPSGIGVA VDDDGRVIGG VLAADVLAAV ESQRKSR

NT Sequence

ATGCCGCACC AGAGGGTGAT CGACAACGTG GCAACGGTTC CCGTGCTGCG CGGCCAGCGT CGGCGGGCCG CCCGCACGGC GGCCTACCAG GTGCTCGAGC GCGTCGGGCT GGACGTCAAG TTGGCCCGGC GCTATCCCGC CCAGCTCTCC GGCGGGGAAC AACAACGGGT CGGCGTGGCT CGCGCGCTGG CCGCCGACCC GCCGATCCTG CTGATGGACG AGCCGTTCTC TGCCGTCGAT CCGGTGGTGC GTCACGACCT GCAAAACGAA ATACTGCGGC TGCAAAGCGA ATTGCACAAA ACCATCGTCT TCGTCACGCA CGACATCGAC GAGGCGCTGC GGCTCGGCGA ACGGGTCGCG GTGTTCAAGG GCGGCGTGTT GCAGCAGTGC GACGAGCCCG CCCGGCTGCT GTCCCGCCCG GCCAACGACT TCGTGTCGAG GTTCATCGGT CTGGGCCGTG GCTACCGATG GTTGCAACTC ATCGACGCCG CCGGGCTGCC GGTGCACGAC GTCCCCCGCC TGTCCGCCGA AAACCTGTCG GGTGCAGATG TTTTCGATGG TTGGGCCGTC GTGGTCGACG AGGCCGGGGC GCCGCTGGGC TGGATCGACG CCGAGGGCCT GCGCCGACAC CGCGACGGCC TGGCGCTGTC CGACAGCATG ACCGGCGTCG GATCGCTGTT TCGGCCGCGG GGCAACCTGA GCCAGGCGCT GGACGCCGCG CTGTCGTCGC CGTCGGGAAT CGGTGTCGCC GTCGACGACG ACGGCAGGGT GATCGGCGGT GTGCTGGCCG CCGACGTGCT GGCCGCGGTG GAATCGCAGC GAAAGAGCCG C

Details for MypaA.01609.a.A1.GE29620

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPHQRVIDN VATVPVLRGQ RRRAARTAAY QVLERVGLDV KLARRYPAQL SGGEQQRVGV ARALAADPPI LLMDEPFSAV DPVVRHDLQN EILRLQSELH KTIVFVTHDI DEALRLGERV AVFKGGVLQQ CDEPARLLSR PANDFVSRFI GLGRGYRWLQ LIDAAGLPVH DVPRLSAENL SGADVFDGWA VVVDEAGAPL GWIDAEGLRR HRDGLALSDS MTGVGSLFRP RGNLSQALDA ALSSPSGIGV AVDDDGRVIG GVLAADVLAA VESQRKS

Validated NT Sequence

cggctctttc gctgcgattc caccgcggcc agcacgtcgg cggccagcac accgccgatc accctgccgt cgtcgtcgac ggcgacaccg attcccgacg gcgacgacag cgcggcgtcc agcgcctggc tcaggttgcc ccgcggccga aacagcgatc cgacgccggt catgctgtcg gacagcgcca ggccgtcgcg gtgtcggcgc aggccctcgg cgtcgatcca gcccagcggc gccccggcct cgtcgaccac gacggcccaa ccatcgaaaa catctgcacc cgacaggttt tcggcggaca ggcgggggac gtcgtgcacc ggcagcccgg cggcgtcgat gagttgcaac catcggtagc cacggcccag accgatgaac ctcgacacga agtcgttggc cgggcgggac agcagccggg cgggctcgtc gcactgctgc aacacgccgc ccttgaacac cgcgacccgt tcgccgagcc gcagcgcctc gtcgatgtcg tgcgtgacga agacgatggt tttgtgcaat tcgctttgca gccgcagtat ttcgttttgc aggtcgtgac gcaccaccgg atcgacggca gagaacggct cgtccatcag caggatcggc gggtcggcgg ccagcgcgcg agccacgccg acccgttgtt gttccccgcc ggagagctgg gcgggatagc gccgggccaa cttgacgtcc agcccgacgc gctcgagcac ctggtaggcc gccgtgcggg cggcccgccg acgctggccg cgcagcacgg gaaccgttgc cacgttgtcg atcaccctct ggtgcggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcaccag agggtgatcg acaacgtggc aacggttccc gtgctgcgcg gccagcgtcg gcgggccgcc cgcacggcgg cctaccaggt gctcgagcgc gtcgggctgg acgtcaagtt ggcccggcgc tatcccgccc agctctccgg cggggaacaa caacgggtcg gcgtggctcg cgcgctggcc gccgacccgc cgatcctgct gatggacgag ccgttctctg ccgtcgatcc ggtggtgcgt cacgacctgc aaaacgaaat actgcggctg caaagcgaat tgcacaaaac catcgtcttc gtcacgcacg acatcgacga ggcgctgcgg ctcggcgaac gggtcgcggt gttcaagggc ggcgtgttgc agcagtgcga cgagcccgcc cggctgctgt cccgcccggc caacgacttc gtgtcgaggt tcatcggtct gggccgtggc taccgatggt tgcaactcat cgacgccgcc gggctgccgg tgcacgacgt cccccgcctg tccgccgaaa acctgtcggg tgcagatgtt ttcgatggtt gggccgtcgt ggtcgacgag gccggggcgc cgctgggctg gatcgacgcc gagggcctgc gccgacaccg cgacggcctg gcgctgtccg acagcatgac cggcgtcgga tcgctgtttc ggccgcgggg caacctgagc caggcgctgg acgccgcgct gtcgtcgccg tcgggaatcg gtgtcgccgt cgacgacgac ggcagggtga tcggcggtgt gctggccgcc gacgtgctgg ccgcggtgga atcgcagcga aagagccgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a