MytuD.01582.a

3-ketoacyl-(acyl-carrier-protein) reductase

CENTER ID: MytuD.01582.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01582.a.MB1.GE30402 Full length( MytuD.01582.a ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3909
RefSeq: NP_218019.1
TubercuList: Rv3502c
MTB Network Portal: Rv3502c
UniProt: O53547

Orthologues for Rv3502c

Species % Identity % Coverage Clones Proteins Structures
marinum 81 96 1 1 0
ulcerans 81 95 0 0 0
paratuberculosis 80 97 0 0 0
avium 80 93 0 0 0
smegmatis 72 98 0 1 1
abscessus 70 95 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLTESNRSP RTTNTTDLSG KVAVVTGAAA GLGRAEALGL ARLGATVVVN DVASALDASD VVDEIGAAAA DAGAKAVAVA GDISQRATAD ELLASAVGLG GLDIVVNNAG ITRDRMLFNM SDEEWDAVIA VHLRGHFLLT RNAAAYWRDK AKDAEGGSVF GRLVNTSSEA GLVGPVGQAN YAAAKAGITA LTLSAARALG RYGVCANVIC PRARTAMTAD VFGAAPDVEA GQIDPLSPQH VVSLVQFLAS PAAAEVNGQV FIVYGPQVTL VSPPHMERRF SADGTSWDPT ELTATLRDYF AGRDPEQSFS ATDLMRQ
NT Sequence
TTGAAATTGA CTGAGTCCAA TCGCTCGCCG CGTACCACGA ACACGACCGA TCTGTCCGGA AAGGTGGCGG TGGTCACCGG CGCGGCCGCG GGTTTGGGTC GGGCCGAAGC GCTCGGCCTG GCCCGGTTGG GCGCCACCGT CGTCGTCAAC GATGTTGCTT CCGCCCTGGA TGCTTCCGAC GTCGTCGACG AGATCGGCGC CGCCGCTGCC GATGCTGGTG CTAAGGCCGT CGCGGTAGCC GGTGATATCA GCCAGCGCGC GACGGCCGAC GAACTGCTCG CCAGCGCCGT CGGGCTGGGC GGACTGGACA TCGTGGTGAA CAATGCCGGC ATCACCCGCG ACCGGATGCT GTTCAACATG TCCGACGAGG AGTGGGACGC CGTGATCGCG GTGCACCTGC GCGGCCATTT TCTGCTCACC CGCAACGCCG CTGCCTACTG GCGGGACAAA GCCAAGGATG CCGAAGGGGG ATCGGTCTTC GGCCGGCTCG TCAACACCTC GTCGGAGGCG GGTCTGGTGG GCCCGGTGGG GCAGGCGAAT TACGCCGCCG CCAAGGCTGG CATCACCGCG CTAACCCTGT CGGCGGCGCG GGCGCTCGGG CGCTACGGCG TTTGCGCCAA TGTGATTTGT CCGCGGGCGC GCACCGCGAT GACGGCCGAT GTCTTCGGCG CCGCACCCGA TGTCGAAGCG GGCCAGATCG ACCCGCTGTC GCCGCAGCAT GTGGTAAGCC TGGTCCAGTT TCTGGCGTCC CCGGCTGCCG CGGAAGTCAA CGGTCAGGTG TTCATCGTCT ACGGTCCGCA GGTGACGCTG GTGTCACCGC CGCACATGGA GCGCCGGTTC AGCGCGGACG GCACGTCCTG GGATCCCACC GAGCTCACCG CGACGCTGCG GGACTACTTT GCTGGTCGGG ATCCGGAACA GAGCTTTTCG GCGACCGATC TGATGCGTCA G
Details for MytuD.01582.a.MB1.GE30402
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 77kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 35
Validated AA Sequence
MLKLTESNRS PRTTNTTDLS GKVAVVTGAA AGLGRAEALG LARLGATVVV NDVASALDAS DVVDEIGAAA ADAGAKAVAV AGDISQRATA DELLASAVGL GGLDIVVNNA GITRDRMLFN MSDEEWDAVI AVHLRGHFLL TRNAAAYWRD KAKDAEGGSV FGRLVNTSSE AGLVGPVGQA NYAAAKAGIT ALTLSAARAL GRYGVCANVI CPRARTAMTA DVFGAAPDVE AGQIDPLSPQ HVVSLGPVSG RPRLPRKSTG
Validated NT Sequence
gggtcctggt tcgatgttga aattgactga gtccaatcgc tcgccgcgta ccacgaacac gaccgatctg tccggaaagg tggcggtggt caccggcgcg gccgcgggtt tgggtcgggc cgaagcgctc ggcctggccc ggttgggcgc caccgtcgtc gtcaacgatg ttgcttccgc cctggatgct tccgacgtcg tcgacgagat cggcgccgcc gctgccgatg ctggtgctaa ggccgtcgcg gtagccggtg atatcagcca gcgcgcgacg gccgacgaac tgctcgccag cgccgtcggg ctgggcggac tggacatcgt ggtgaacaat gccggcatca cccgcgaccg gatgctgttc aacatgtccg acgaggagtg ggacgccgtg atcgcggtgc acctgcgcgg ccattttctg ctcacccgca acgccgctgc ctactggcgg gacaaagcca aggatgccga agggggatcg gtcttcggcc ggctcgtcaa cacctcgtcg gaggcgggtc tggtgggccc ggtggggcag gcgaattacg ccgccgccaa ggctggcatc accgcgctaa ccctgtcggc ggcgcgggcg ctcgggcgct acggcgtttg cgccaatgtg atttgtccgc gggcgcgcac cgcgatgacg gccgatgtct tcggcgccgc acccgatgtc gaagcgggcc agatcgaccc gctgtcgccg cagcatgtgg taagcctggg tccagtttct gggcgtcccc ggctgccgcg gaagtcaacg ggtca
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MLKLTESNRS PRTTNTTDLS GKVAVVTGAA AGLGRAEALG LARLGATVVV NDVASALDAS DVVDEIGAAA ADAGAKAVAV AGDISQRATA DELLASAVGL GGLDIVVNNA GITRDRMLFN MSDEEWDAVI AVHLRGHFLL TRNAAAYWRD KAKDAEGGSV FGRLVNTSSE AGLVGPVGQA NYAAAKAGIT ALTLSAARAL GRYGVCANVI CPRARTAMTA DVFGAAPDVE AGQIDPLSPQ HVVSLVQFLA SPAAAEVNGQ VFIVYGPQVT LVSPPHMERR FSADGTSWDP TELTATLRDY FAGRDPEQSF SATDLMRQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgtt gaaattgact gagtccaatc gctcgccgcg taccacgaac acgaccgatc tgtccggaaa ggtggcggtg gtcaccggcg cggccgcggg tttgggtcgg gccgaagcgc tcggcctggc ccggttgggc gccaccgtcg tcgtcaacga tgttgcttcc gccctggatg cttccgacgt cgtcgacgag atcggcgccg ccgctgccga tgctggtgct aaggccgtcg cggtagccgg tgatatcagc cagcgcgcga cggccgacga actgctcgcc agcgccgtcg ggctgggcgg actggacatc gtggtgaaca atgccggcat cacccgcgac cggatgctgt tcaacatgtc cgacgaggag tgggacgccg tgatcgcggt gcacctgcgc ggccattttc tgctcacccg caacgccgct gcctactggc gggacaaagc caaggatgcc gaagggggat cggtcttcgg ccggctcgtc aacacctcgt cggaggcggg tctggtgggc ccggtggggc aggcgaatta cgccgccgcc aaggctggca tcaccgcgct aaccctgtcg gcggcgcggg cgctcgggcg ctacggcgtt tgcgccaatg tgatttgtcc gcgggcgcgc accgcgatga cggccgatgt cttcggcgcc gcacccgatg tcgaagcggg ccagatcgac ccgctgtcgc cgcagcatgt ggtaagcctg gtccagtttc tggcgtcccc ggctgccgcg gaagtcaacg gtcaggtgtt catcgtctac ggtccgcagg tgacgctggt gtcaccgccg cacatggagc gccggttcag cgcggacggc acgtcctggg atcccaccga gctcaccgcg acgctgcggg actactttgc tggtcgggat ccggaacaga gcttttcggc gaccgatctg atgcgtcagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa