MysmA.01582.a
Oxidoreductase, short chain dehydrogenase/reductase family protein
| CENTER ID: | MysmA.01582.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01582.a.A1.PS00637 | Full length( MysmA.01582.a ) | 1 | 316 |
Structures
4KZP
DEPOSITED: 5/30/2013
DETERMINATION: XRay
CLONE: MysmA.01582.a.A1.GE28953
PROTEIN: MysmA.01582.a.A1.PS00637
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_239168 |
| PATRIC ID: | fig|246196.19.peg.5745 |
| RefSeq: | YP_890129.1 |
| UniProt: | A0R4P1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNDSHPSDT GPDRGADLDL SGKVAVVTGA AAGLGRAEAI GLAKAGATVV VNDIAGALEG SDVLDEIAAA GSKGVAVAGD ISQRSTADEL VETAEGLGGL DIVVNNAGIT RDRILFNMSD EEWDAVIAVH LRGHFLLTRN AAAYWRSKAK AGDGTVYGRV INTSSEAGLS GPVGQPNYGA AKAGITALTL SAARALERFG VRANAIAPRA RTAMTAGVFG DAPELAEGQM DPLSTDHVVT LVQFLAAPAS EGVNGQLFIV YGPSVTLVAA PTAEKQFVAD EDAWEPADLS VTLRDYFADR DPERGFSATA LMESRD
NT Sequence
ATGACCAACG ACAGCCACCC CTCCGACACC GGGCCCGATC GAGGGGCCGA TCTCGACCTG TCCGGAAAGG TCGCCGTGGT CACCGGCGCC GCAGCGGGCC TCGGCCGGGC CGAGGCGATC GGGCTGGCCA AGGCCGGCGC CACGGTCGTG GTCAACGACA TCGCGGGCGC GCTCGAGGGG TCCGACGTTC TCGACGAGAT CGCAGCGGCC GGCTCCAAGG GTGTGGCCGT GGCGGGCGAC ATCAGTCAGC GGTCCACCGC CGACGAGCTC GTCGAGACCG CCGAGGGGCT CGGCGGCCTG GACATCGTCG TCAACAATGC CGGCATCACG CGTGACCGCA TCCTGTTCAA CATGAGCGAC GAGGAGTGGG ACGCGGTCAT CGCGGTCCAC CTGCGCGGGC ACTTCCTGCT GACCCGCAAC GCCGCCGCCT ACTGGCGGAG CAAGGCCAAG GCGGGCGACG GGACGGTCTA CGGCCGCGTC ATCAACACGT CGTCGGAGGC CGGGCTGTCC GGTCCGGTCG GTCAGCCCAA CTACGGCGCG GCCAAGGCCG GTATCACCGC GCTCACGCTG TCGGCGGCCA GGGCGCTGGA GCGCTTCGGC GTCCGGGCCA ACGCGATCGC GCCGCGGGCG CGCACCGCGA TGACCGCGGG GGTGTTCGGC GATGCGCCCG AGCTGGCCGA GGGGCAGATG GACCCACTGT CGACCGATCA TGTCGTGACA CTCGTGCAGT TCCTGGCGGC ACCGGCCTCA GAAGGTGTGA ACGGTCAGCT GTTCATCGTT TATGGTCCAT CTGTCACCTT GGTTGCCGCA CCCACCGCGG AGAAGCAGTT CGTCGCCGAC GAGGACGCGT GGGAACCGGC AGACCTCAGC GTGACGCTGC GGGACTACTT TGCTGATCGG GATCCGGAGC GTGGGTTCTC GGCGACTGCA CTGATGGAGT CGCGAGAC
Details for MysmA.01582.a.A1.PS00637
| PURIFICATION DATe: | 6/17/2010 |
| CONCENTRATION: | 60.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXTXAXPXTP ARSRGXISTC PXRSPWSPAP QRASXRAEAI GLAKAGATVV VNDIAGALEG SDVLDEIAAA GSKGVAVAGD ISQRSTADEL VETAEGLGGL DIVVNNAGIT RDRILFNMSD EEWDAVIAVH LRGHFLLTRN AAAYWRSKAK AGDGTVYGRV INTSSEAGLS GPVGQPNYGA AKAGITALTL SAARALERFG VRANAIAPRA RTAMTAGVFG DAPELAEGQM DPLSTDHVVT LVQFLAAPAS EGVNGQLFIV YGPSVTLVAA PTAEKQFVAD EDAWEPADLS VTLRDYFADR DPERGFSATA LMESRD
Validated NT Sequence
ttcgnnnttt gttagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattagtc tcgcgactcc atcagtgcag tcgccgagaa cccacgctcc ggatcccgat cagcaaagta gtcccgcagc gtcacgctga ggtctgccgg ttcccacgcg tcctcgtcgg cgacgaactg cttctccgcg gtgggtgcgg caaccaaggt gacagatgga ccataaacga tgaacagctg accgttcaca ccttctgagg ccggtgccgc caggaactgc acgagtgtca cgacatgatc ggtcgacagt gggtccatct gcccctcggc cagctcgggc gcatcgccga acacccccgc ggtcatcgcg gtgcgcgccc gcggcgcgat cgcgttggcc cggacgccga agcgctccag cgccctggcc gccgacagcg tgagcgcggt gataccggcc ttggccgcgc cgtagttggg ctgaccgacc ggaccggaca gcccggcctc cgacgacgtg ttgatgacgc ggccgtagac cgtcccgtcg cccgccttgg ccttgctccg ccagtaggcg gcggcgttgc gggtcagcag gaagtgcccg cgcaggtgga ccgcgatgac cgcgtcccac tcctcgtcgc tcatgttgaa caggatgcgg tcacgcgtga tgccggcatt gttgacgacg atgtccaggc cgccgagccc ctcggcggtc tcgacgagct cgtcggcggt ggaccgctga ctgatgtcgc ccgccacggc cacacccttg gagccggccg ctgcgatctc gtcgagaacg tcggacccct cgagcgcgcc cgcgatgtcg ttgaccacga ccgtggcgcc ggccttggcc agcccgatcg cctcggcccg gnccgaggcc cgctgcggcg ccggtgacca cggcgacctt tncggacagg tcgagatcng ncccctcgat cgggccggtg tcgnaggggn ngctgncgtn gncatcgaac cnnnncctgg gtctganntc aggnaccata tgatgnnatg nnatgancnt nnnnntcnnn ntnannnnaa annntnnaga gnnnngnnnn gna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNDSHPSD TGPDRGADLD LSGKVAVVTG AAAGLGRAEA IGLAKAGATV VVNDIAGALE GSDVLDEIAA AGSKGVAVAG DISQRSTADE LVETAEGLGG LDIVVNNAGI TRDRILFNMS DEEWDAVIAV HLRGHFLLTR NAAAYWRSKA KAGDGTVYGR VINTSSEAGL SGPVGQPNYG AAKAGITALT LSAARALERF GVRANAIAPR ARTAMTAGVF GDAPELAEGQ MDPLSTDHVV TLVQFLAAPA SEGVNGQLFI VYGPSVTLVA APTAEKQFVA DEDAWEPADL SVTLRDYFAD RDPERGFSAT ALMESRD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAACGAC AGCCACCCCT CCGACACCGG GCCCGATCGA GGGGCCGATC TCGACCTGTC CGGAAAGGTC GCCGTGGTCA CCGGCGCCGC AGCGGGCCTC GGCCGGGCCG AGGCGATCGG GCTGGCCAAG GCCGGCGCCA CGGTCGTGGT CAACGACATC GCGGGCGCGC TCGAGGGGTC CGACGTTCTC GACGAGATCG CAGCGGCCGG CTCCAAGGGT GTGGCCGTGG CGGGCGACAT CAGTCAGCGG TCCACCGCCG ACGAGCTCGT CGAGACCGCC GAGGGGCTCG GCGGCCTGGA CATCGTCGTC AACAATGCCG GCATCACGCG TGACCGCATC CTGTTCAACA TGAGCGACGA GGAGTGGGAC GCGGTCATCG CGGTCCACCT GCGCGGGCAC TTCCTGCTGA CCCGCAACGC CGCCGCCTAC TGGCGGAGCA AGGCCAAGGC GGGCGACGGG ACGGTCTACG GCCGCGTCAT CAACACGTCG TCGGAGGCCG GGCTGTCCGG TCCGGTCGGT CAGCCCAACT ACGGCGCGGC CAAGGCCGGT ATCACCGCGC TCACGCTGTC GGCGGCCAGG GCGCTGGAGC GCTTCGGCGT CCGGGCCAAC GCGATCGCGC CGCGGGCGCG CACCGCGATG ACCGCGGGGG TGTTCGGCGA TGCGCCCGAG CTGGCCGAGG GGCAGATGGA CCCACTGTCG ACCGATCATG TCGTGACACT CGTGCAGTTC CTGGCGGCAC CGGCCTCAGA AGGTGTGAAC GGTCAGCTGT TCATCGTTTA TGGTCCATCT GTCACCTTGG TTGCCGCACC CACCGCGGAG AAGCAGTTCG TCGCCGACGA GGACGCGTGG GAACCGGCAG ACCTCAGCGT GACGCTGCGG GACTACTTTG CTGATCGGGA TCCGGAGCGT GGGTTCTCGG CGACTGCACT GATGGAGTCG CGAGACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA