SSGCID: Target Page

MymaA.01582.a

Short-chain type dehydrogenase/reductase

CENTER ID:	MymaA.01582.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01582.a.A1.GE30195	Full length( MymaA.01582.a )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01582.a.A1.PW30068	Full length( MymaA.01582.a )		1	303

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5344
RefSeq:	YP_001853249.1
UniProt:	B2HHZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSSTNVTDL SGRVAVVTGA AAGLGRAEAV GLARLGATVV VNDIGSALDA SDVIDEISAV GAKAIAVAGD ISQRATADEL IATADGLGGL DVVVNNAGIT RDRMLFNMTD EDWDQVIGVH LRGHFLLTRN AATYWRQKAK AAGGSIFGRL VNTSSEAGLV GPVGQANYGA AKAGITALTL SAARALGRYG VCANAICPRA RTAMTADVFG ATPDVAAGEI DPLSPEHVVT LVQFLASPAA AAVNGQLFIV YGPQVTLVAA PTAEHKFSAD GPAWEPGQLT DTLHDYFAGR NPEHNFSASS LMA

NT Sequence

TTGACTAGCA GCACGAACGT GACCGATCTG TCCGGAAGGG TCGCGGTGGT TACCGGCGCG GCCGCGGGAC TGGGCCGTGC CGAGGCGGTC GGCCTGGCCC GGCTCGGCGC CACCGTGGTG GTCAACGACA TCGGCTCGGC CTTGGACGCC TCTGACGTCA TTGATGAGAT CAGCGCCGTC GGTGCCAAGG CGATTGCGGT GGCCGGCGAC ATCAGCCAGC GCGCCACCGC CGATGAACTG ATTGCCACCG CCGATGGGCT GGGCGGGCTC GACGTCGTGG TCAACAACGC CGGCATCACC CGCGACCGGA TGCTGTTCAA CATGACCGAC GAGGACTGGG ACCAGGTCAT CGGGGTGCAC CTGCGCGGCC ACTTCCTGCT GACCCGCAAT GCGGCAACCT ATTGGCGCCA GAAAGCCAAG GCGGCCGGGG GCTCGATATT TGGGCGGCTG GTCAACACCT CATCGGAAGC GGGGCTGGTC GGTCCCGTCG GGCAGGCCAA CTACGGCGCC GCCAAGGCCG GCATCACCGC ACTCACCCTG TCCGCGGCGC GGGCGTTGGG CCGTTACGGC GTATGCGCCA ACGCGATCTG TCCGCGGGCC CGCACCGCCA TGACCGCCGA CGTCTTCGGG GCGACGCCCG ACGTGGCGGC GGGCGAGATC GACCCGTTGT CGCCGGAACA CGTGGTGACT CTGGTGCAGT TTCTGGCGTC CCCGGCGGCC GCGGCGGTCA ACGGGCAGCT GTTCATCGTC TACGGTCCGC AGGTGACGCT GGTGGCGGCG CCGACGGCGG AGCACAAGTT CAGTGCGGAC GGCCCGGCCT GGGAGCCCGG CCAGCTAACC GACACATTGC ACGATTACTT TGCTGGGCGA AACCCCGAAC ACAACTTTTC CGCTAGCTCG CTGATGGCT

Details for MymaA.01582.a.A1.GE30195

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	33kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLTSSTN VTDLSGRVAV VTGAAAGLGR AEAVGLARLG ATVVVNDIGS ALDASDVIDE ISAVGAKAIA VAGDISQRAT ADELIATADG LGGLDVVVNN AGITRDRMLF NMTDEDWDQV IGVHLRGHFL LTRNAATYWR QKAKAAGGSI FGRLVNTSSE AGLVGPVGQA NYGAAKAGIT ALTLSAARAL GRYGVCANAI CPRARTAMTA DVFGATPDVA AGEIDPLSPE HVVTLVQFLA SPAAAAVNGQ LFIVYGPQVT LVAAPTAEHK FSADGPAWEP GQLTDTLHDY FAGRNPEHNF SASSLMA

Validated NT Sequence

ttattaagcc atcagcgagc tagcggaaaa gttgtgttcg gggtttcgcc cagcaaagta atcgtgcaat gtgtcggtta gctggccggg ctcccaggcc gggccgtccg cactgaactt gtgctccgcc gtcggcgccg ccaccagcgt cacctgcgga ccgtagacga tgaacagctg cccgttgacc gccgcggccg ccggggacgc cagaaactgc accagagtca ccacgtgttc cggcgacaac gggtcgatct cgcccgccgc cacgtcgggc gtcgccccga agacgtcggc ggtcatggcg gtgcgggccc gcggacagat cgcgttggcg catacgccgt aacggcccaa cgcccgcgcc gcggacaggg tgagtgcggt gatgccggcc ttggcggcgc cgtagttggc ctgcccgacg ggaccgacca gccccgcttc cgatgaggtg ttgaccagcc gcccaaatat cgagcccccg gccgccttgg ctttctggcg ccaataggtt gccgcattgc gggtcagcag gaagtggccg cgcaggtgca ccccgatgac ctggtcccag tcctcgtcgg tcatgttgaa cagcatccgg tcgcgggtga tgccggcgtt gttgaccacg acgtcgagcc cgcccagccc atcggcggtg gcaatcagtt catcggcggt ggcgcgctgg ctgatgtcgc cggccaccgc aatcgccttg gcaccgacgg cgctgatctc atcaatgacg tcagaggcgt ccaaggccga gccgatgtcg ttgaccacca cggtggcgcc gagccgggcc aggccgaccg cctcggcacg gcccagtccc gcggccgcgc cggtaaccac cgcgaccctt ccggacagat cggtcacgtt cgtgctgcta gtcaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLTSSTNVT DLSGRVAVVT GAAAGLGRAE AVGLARLGAT VVVNDIGSAL DASDVIDEIS AVGAKAIAVA GDISQRATAD ELIATADGLG GLDVVVNNAG ITRDRMLFNM TDEDWDQVIG VHLRGHFLLT RNAATYWRQK AKAAGGSIFG RLVNTSSEAG LVGPVGQANY GAAKAGITAL TLSAARALGR YGVCANAICP RARTAMTADV FGATPDVAAG EIDPLSPEHV VTLVQFLASP AAAAVNGQLF IVYGPQVTLV AAPTAEHKFS ADGPAWEPGQ LTDTLHDYFA GRNPEHNFSA SSLMA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgactagc agcacgaacg tgaccgatct gtccggaagg gtcgcggtgg ttaccggcgc ggccgcggga ctgggccgtg ccgaggcggt cggcctggcc cggctcggcg ccaccgtggt ggtcaacgac atcggctcgg ccttggacgc ctctgacgtc attgatgaga tcagcgccgt cggtgccaag gcgattgcgg tggccggcga catcagccag cgcgccaccg ccgatgaact gattgccacc gccgatgggc tgggcgggct cgacgtcgtg gtcaacaacg ccggcatcac ccgcgaccgg atgctgttca acatgaccga cgaggactgg gaccaggtca tcggggtgca cctgcgcggc cacttcctgc tgacccgcaa tgcggcaacc tattggcgcc agaaagccaa ggcggccggg ggctcgatat ttgggcggct ggtcaacacc tcatcggaag cggggctggt cggtcccgtc gggcaggcca actacggcgc cgccaaggcc ggcatcaccg cactcaccct gtccgcggcg cgggcgttgg gccgttacgg cgtatgcgcc aacgcgatct gtccgcgggc ccgcaccgcc atgaccgccg acgtcttcgg ggcgacgccc gacgtggcgg cgggcgagat cgacccgttg tcgccggaac acgtggtgac tctggtgcag tttctggcgt ccccggcggc cgcggcggtc aacgggcagc tgttcatcgt ctacggtccg caggtgacgc tggtggcggc gccgacggcg gagcacaagt tcagtgcgga cggcccggcc tgggagcccg gccagctaac cgacacattg cacgattact ttgctgggcg aaaccccgaa cacaactttt ccgctagctc gctgatggct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MymaA.01582.a.A1.PW30068

PURIFICATION DATe:	10/25/2010
CONCENTRATION:	28.04mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGACTAGC AGCACGAACG TGACCGATCT GTCCGGAAGG GTCGCGGTGG TTACCGGCGC GGCCGCGGGA CTGGGCCGTG CCGAGGCGGT CGGCCTGGCC CGGCTCGGCG CCACCGTGGT GGTCAACGAC ATCGGCTCGG CCTTGGACGC CTCTGACGTC ATTGATGAGA TCAGCGCCGT CGGTGCCAAG GCGATTGCGG TGGCCGGCGA CATCAGCCAG CGCGCCACCG CCGATGAACT GATTGCCACC GCCGATGGGC TGGGCGGGCT CGACGTCGTG GTCAACAACG CCGGCATCAC CCGCGACCGG ATGCTGTTCA ACATGACCGA CGAGGACTGG GACCAGGTCA TCGGGGTGCA CCTGCGCGGC CACTTCCTGC TGACCCGCAA TGCGGCAACC TATTGGCGCC AGAAAGCCAA GGCGGCCGGG GGCTCGATAT TTGGGCGGCT GGTCAACACC TCATCGGAAG CGGGGCTGGT CGGTCCCGTC GGGCAGGCCA ACTACGGCGC CGCCAAGGCC GGCATCACCG CACTCACCCT GTCCGCGGCG CGGGCGTTGG GCCGTTACGG CGTATGCGCC AACGCGATCT GTCCGCGGGC CCGCACCGCC ATGACCGCCG ACGTCTTCGG GGCGACGCCC GACGTGGCGG CGGGCGAGAT CGACCCGTTG TCGCCGGAAC ACGTGGTGAC TCTGGTGCAG TTTCTGGCGT CCCCGGCGGC CGCGGCGGTC AACGGGCAGC TGTTCATCGT CTACGGTCCG CAGGTGACGC TGGTGGCGGC GCCGACGGCG GAGCACAAGT TCAGTGCGGA CGGCCCGGCC TGGGAGCCCG GCCAGCTAAC CGACACATTG CACGATTACT TTGCTGGGCG AAACCCCGAA CACAACTTTT CCGCTAGCTC GCTGATGGCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA