SSGCID: Target Page

MyabA.01582.c

Putative short chain dehydrogenase/reductase

CENTER ID:	MyabA.01582.c
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01582.c.A1.GE30527	Full length( MyabA.01582.c )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01582.c.A1.PW30404	Full length( MyabA.01582.c )		1	301

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4234
RefSeq:	YP_001704883.1
UniProt:	B1MIK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAIADADLS GRVAIVTGAA AGLGRAEAIG LARSGATVVV NDIAPALDKS DVLDEIAAAG SKGVTVAGDI GERATADELI STAESLGGLH VVVNNAGITR DRMLFNMSDE EFDAVIHVHL RGHFLLTRNA ATYWRGASKA AGAPTYGRLI NTSSEAGLLG PEGQANYGAA KAGITALTLS AARALGRYGV RANAIAPRAR TAMTADVFGD APEDSIDPLS PEHVVTLVRY LAAPAAEAVN GQLFIVYGPT VTLLAAPTVE AKFTADSDAW DPSALNSTLA DFFAGHDPKR TFSATALMVE D

NT Sequence

ATGAGCGCGA TTGCTGATGC CGATCTGAGC GGCCGTGTCG CCATTGTCAC GGGTGCTGCC GCGGGACTCG GCCGTGCCGA GGCGATCGGC TTGGCGCGGT CCGGGGCGAC CGTCGTCGTC AACGACATCG CGCCGGCATT GGACAAGAGC GATGTGCTCG ACGAGATCGC CGCGGCGGGA TCCAAGGGTG TCACCGTCGC CGGTGATATC GGCGAGCGTG CGACCGCCGA CGAACTGATC TCCACCGCCG AATCCCTCGG TGGCCTGCAT GTCGTGGTCA ACAATGCGGG CATCACCCGC GATCGGATGC TCTTCAACAT GTCGGACGAG GAGTTCGACG CCGTCATCCA CGTACACCTG CGCGGCCACT TCCTGCTGAC CCGTAACGCG GCCACATACT GGCGTGGAGC CTCCAAGGCG GCCGGAGCCC CCACCTACGG ACGCCTTATC AATACGTCCT CCGAAGCCGG CCTGCTGGGG CCGGAGGGGC AGGCCAACTA CGGCGCCGCC AAGGCGGGTA TCACCGCGCT CACGCTGTCG GCTGCCCGCG CGCTGGGCCG GTACGGGGTG CGGGCCAACG CCATCGCCCC GCGTGCCCGC ACCGCGATGA CGGCCGACGT CTTCGGAGAC GCGCCCGAGG ATTCGATCGA TCCGCTATCC CCTGAGCATG TCGTGACATT GGTGCGCTAT CTGGCCGCGC CGGCGGCGGA GGCGGTCAAT GGTCAGCTAT TCATTGTTTA CGGACCCACC GTGACGTTGT TGGCAGCGCC TACTGTGGAG GCGAAGTTCA CCGCGGATTC GGACGCCTGG GATCCGTCAG CACTGAATTC AACCCTGGCT GATTTCTTTG CTGGACACGA CCCCAAGCGC ACTTTCTCCG CGACTGCGCT GATGGTCGAG GAT

Details for MyabA.01582.c.A1.GE30527

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMSAIADA DLSGRVAIVT GAAAGLGRAE AIGLARSGAT VVVNDIAPAL DKSDVLDEIA AAGSKGVTVA GDIGERATAD ELISTAESLG GLHVVVNNAG ITRDRMLFNM SDEEFDAVIH VHLRGHFLLT RNAATYWRGA SKAAGAPTYG RLINTSSEAG LLGPEGQANY GAAKAGITAL TLSAARALGR YGVRANAIAP RARTAMTADV FGDAPEDSID PLSPEHVVTL VRYLAAPAAE AVNGQLFIVY GPTVTLLAAP TVEAKFTADS DAWDPSALNS TLADFFAGHD PKRTFSATAL MV

Validated NT Sequence

cgaccatcag cgcagtcgcg gagaaagtgc gcttggggtc gtgtccagca aagaaatcag ccagggttga attcagtgct gacggatccc aggcgtccga atccgcggtg aacttcgcct ccacagtagg cgctgccaac aacgtcacgg tgggtccgta aacaatgaat agctgaccat tgaccgcctc cgccgccggc gcggccagat agcgcaccaa tgtcacgaca tgctcagggg atagcggatc gatcgaatcc tcgggcgcgt ctccgaagac gtcggccgtc atcgcggtgc gggcacgcgg ggcgatggcg ttggcccgca ccccgtaccg gcccagcgcg cgggcagccg acagcgtgag cgcggtgata cccgccttgg cggcgccgta gttggcctgc ccctccggcc ccagcaggcc ggcttcggag gacgtattga taaggcgtcc gtaggtgggg gctccggccg ccttggaggc tccacgccag tatgtggccg cgttacgggt cagcaggaag tggccgcgca ggtgtacgtg gatgacggcg tcgaactcct cgtccgacat gttgaagagc atccgatcgc gggtgatgcc cgcattgttg accacgacat gcaggccacc gagggattcg gcggtggaga tcagttcgtc ggcggtcgca cgctcgccga tatcaccggc gacggtgaca cccttggatc ccgccgcggc gatctcgtcg agcacatcgc tcttgtccaa tgccggcgcg atgtcgttga cgacgacggt cgccccggac cgcgccaagc cgatcgcctc ggcacggccg agtcccgcgg cagcacccgt gacaatggcg acacggccgc tcagatcggc atcagcaatc gcgctcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSAIADADL SGRVAIVTGA AAGLGRAEAI GLARSGATVV VNDIAPALDK SDVLDEIAAA GSKGVTVAGD IGERATADEL ISTAESLGGL HVVVNNAGIT RDRMLFNMSD EEFDAVIHVH LRGHFLLTRN AATYWRGASK AAGAPTYGRL INTSSEAGLL GPEGQANYGA AKAGITALTL SAARALGRYG VRANAIAPRA RTAMTADVFG DAPEDSIDPL SPEHVVTLVR YLAAPAAEAV NGQLFIVYGP TVTLLAAPTV EAKFTADSDA WDPSALNSTL ADFFAGHDPK RTFSATALMV ED

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgatt gctgatgccg atctgagcgg ccgtgtcgcc attgtcacgg gtgctgccgc gggactcggc cgtgccgagg cgatcggctt ggcgcggtcc ggggcgaccg tcgtcgtcaa cgacatcgcg ccggcattgg acaagagcga tgtgctcgac gagatcgccg cggcgggatc caagggtgtc accgtcgccg gtgatatcgg cgagcgtgcg accgccgacg aactgatctc caccgccgaa tccctcggtg gcctgcatgt cgtggtcaac aatgcgggca tcacccgcga tcggatgctc ttcaacatgt cggacgagga gttcgacgcc gtcatccacg tacacctgcg cggccacttc ctgctgaccc gtaacgcggc cacatactgg cgtggagcct ccaaggcggc cggagccccc acctacggac gccttatcaa tacgtcctcc gaagccggcc tgctggggcc ggaggggcag gccaactacg gcgccgccaa ggcgggtatc accgcgctca cgctgtcggc tgcccgcgcg ctgggccggt acggggtgcg ggccaacgcc atcgccccgc gtgcccgcac cgcgatgacg gccgacgtct tcggagacgc gcccgaggat tcgatcgatc cgctatcccc tgagcatgtc gtgacattgg tgcgctatct ggccgcgccg gcggcggagg cggtcaatgg tcagctattc attgtttacg gacccaccgt gacgttgttg gcagcgccta ctgtggaggc gaagttcacc gcggattcgg acgcctggga tccgtcagca ctgaattcaa ccctggctga tttctttgct ggacacgacc ccaagcgcac tttctccgcg actgcgctga tggtcgagga ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MyabA.01582.c.A1.PW30404

PURIFICATION DATe:	12/20/2010
CONCENTRATION:	27.93mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCGATT GCTGATGCCG ATCTGAGCGG CCGTGTCGCC ATTGTCACGG GTGCTGCCGC GGGACTCGGC CGTGCCGAGG CGATCGGCTT GGCGCGGTCC GGGGCGACCG TCGTCGTCAA CGACATCGCG CCGGCATTGG ACAAGAGCGA TGTGCTCGAC GAGATCGCCG CGGCGGGATC CAAGGGTGTC ACCGTCGCCG GTGATATCGG CGAGCGTGCG ACCGCCGACG AACTGATCTC CACCGCCGAA TCCCTCGGTG GCCTGCATGT CGTGGTCAAC AATGCGGGCA TCACCCGCGA TCGGATGCTC TTCAACATGT CGGACGAGGA GTTCGACGCC GTCATCCACG TACACCTGCG CGGCCACTTC CTGCTGACCC GTAACGCGGC CACATACTGG CGTGGAGCCT CCAAGGCGGC CGGAGCCCCC ACCTACGGAC GCCTTATCAA TACGTCCTCC GAAGCCGGCC TGCTGGGGCC GGAGGGGCAG GCCAACTACG GCGCCGCCAA GGCGGGTATC ACCGCGCTCA CGCTGTCGGC TGCCCGCGCG CTGGGCCGGT ACGGGGTGCG GGCCAACGCC ATCGCCCCGC GTGCCCGCAC CGCGATGACG GCCGACGTCT TCGGAGACGC GCCCGAGGAT TCGATCGATC CGCTATCCCC TGAGCATGTC GTGACATTGG TGCGCTATCT GGCCGCGCCG GCGGCGGAGG CGGTCAATGG TCAGCTATTC ATTGTTTACG GACCCACCGT GACGTTGTTG GCAGCGCCTA CTGTGGAGGC GAAGTTCACC GCGGATTCGG ACGCCTGGGA TCCGTCAGCA CTGAATTCAA CCCTGGCTGA TTTCTTTGCT GGACACGACC CCAAGCGCAC TTTCTCCGCG ACTGCGCTGA TGGTCGAGGA TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA