MytuD.01353.a
O-succinylhomoserine sulfhydrylase
| CENTER ID: | MytuD.01353.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01353.a.B1.GE27179 | Full length( MytuD.01353.a ) | 1 | 406 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01353.a.B1.PW27168 | Full length( MytuD.01353.a ) | 1 | 406 |
Structures
3NDN
DEPOSITED: 6/7/2010
DETERMINATION: XRay
CLONE: MytuD.01353.a.B1.GE27179
PROTEIN: MytuD.01353.a.B1.PW27168
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.430 |
| RefSeq: | NP_214905.1 |
| TubercuList: | Rv0391 |
| MTB Network Portal: | Rv0391 |
| UniProt: | P9WGB5 |
Orthologues for Rv0391
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| avium | 92 | 99 | 1 | 1 | 0 |
| paratuberculosis | 91 | 99 | 0 | 0 | 0 |
| leprae | 88 | 100 | 1 | 0 | 0 |
| marinum | 87 | 100 | 1 | 0 | 0 |
| smegmatis | 86 | 100 | 0 | 0 | 0 |
| thermoresistibile | 85 | 100 | 0 | 0 | 0 |
| abscessus | 83 | 99 | 0 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDESSVRTP KALPDGVSQA TVGVRGGMLR SGFEETAEAM YLTSGYVYGS AAVAEKSFAG ELDHYVYSRY GNPTVSVFEE RLRLIEGAPA AFATASGMAA VFTSLGALLG AGDRLVAARS LFGSCFVVCS EILPRWGVQT VFVDGDDLSQ WERALSVPTQ AVFFETPSNP MQSLVDIAAV TELAHAAGAK VVLDNVFATP LLQQGFPLGV DVVVYSGTKH IDGQGRVLGG AILGDREYID GPVQKLMRHT GPAMSAFNAW VLLKGLETLA IRVQHSNASA QRIAEFLNGH PSVRWVRYPY LPSHPQYDLA KRQMSGGGTV VTFALDCPED VAKQRAFEVL DKMRLIDISN NLGDAKSLVT HPATTTHRAM GPEGRAAIGL GDGVVRISVG LEDTDDLIAD IDRALS
NT Sequence
ATGACCGACG AGTCTTCGGT CCGCACCCCG AAGGCGCTGC CCGACGGCGT CAGCCAGGCC ACCGTCGGGG TGCGCGGCGG GATGTTGCGG TCGGGGTTCG AAGAGACCGC CGAGGCGATG TACCTGACGT CCGGATATGT CTACGGCTCG GCGGCGGTTG CCGAGAAGTC GTTCGCTGGC GAGCTGGACC ACTATGTGTA CTCCCGCTAC GGCAACCCAA CGGTGTCGGT GTTCGAGGAG CGGCTGCGGC TGATCGAGGG TGCCCCGGCG GCGTTCGCCA CCGCCAGTGG CATGGCCGCG GTATTCACCT CGCTGGGCGC GCTGCTGGGT GCCGGAGACC GACTGGTTGC CGCGCGCAGC CTGTTTGGCT CGTGTTTCGT GGTGTGCAGC GAGATCCTGC CGCGCTGGGG GGTGCAGACC GTCTTCGTCG ACGGTGACGA CCTCTCGCAA TGGGAGCGGG CGCTTTCGGT ACCCACGCAG GCCGTGTTCT TCGAGACGCC GTCCAATCCC ATGCAGTCGC TGGTGGATAT CGCTGCGGTG ACCGAGCTGG CACATGCCGC GGGTGCAAAA GTGGTGCTGG ACAACGTATT TGCCACACCG CTACTGCAGC AGGGCTTTCC GCTGGGGGTC GACGTGGTGG TGTACTCGGG CACCAAGCAC ATCGACGGTC AGGGTCGGGT GCTGGGCGGG GCCATACTCG GTGACCGGGA GTACATCGAC GGTCCGGTGC AAAAGCTGAT GCGCCACACC GGTCCGGCGA TGAGTGCGTT CAACGCCTGG GTACTGTTGA AAGGCCTTGA GACGCTGGCT ATTCGGGTGC AACACAGCAA TGCCTCGGCG CAGCGGATCG CGGAGTTCCT CAACGGCCAT CCCTCGGTTC GGTGGGTGCG TTACCCGTAC CTGCCGTCGC ACCCACAATA TGACCTGGCC AAGCGTCAGA TGTCCGGTGG CGGAACCGTC GTTACCTTCG CACTCGACTG CCCGGAGGAT GTTGCCAAAC AGCGGGCCTT CGAGGTGCTC GACAAGATGC GGCTGATCGA CATCTCCAAC AACCTCGGCG ACGCCAAATC GCTTGTCACC CACCCCGCCA CCACGACGCA CCGGGCGATG GGCCCGGAGG GCCGGGCCGC GATCGGGCTC GGTGACGGTG TGGTCCGCAT CTCGGTTGGG TTGGAAGACA CCGACGACCT GATTGCCGAT ATCGATCGGG CGTTGAGC
Details for MytuD.01353.a.B1.GE27179
| HARVESTED ON: | 8/17/2009 |
| SEQUENCED ON: | 9/29/2009 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT DESSVRTPKA LPDGVSQATV GVRGGMLRSG FEETAEAMYL TSGYVYGSAA VAEKSFAGEL DHYVYSRYGN PTVSVFEERL RLIEGAPAAF ATASGMAAVF TSLGALLGAG DRLVAARSLF GSCFVVCSEI LPRWGVQTVF VDGDDLSQWE RALSVPTQAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGFPLGVDV VVYSGTKHID GQGRVLGGAI LGDREYIDGP VQKLMRHTGP AMSAFNAWVL LKGLETLAIR VQHSNASAQR IAEFLNGHPS VRWVRYPYLP SHPQYDLAKR QMSGGGTVVT FALDCPEDVA KQRAFEVLDK MRLIDISNNL GDAKSLVTHP ATTTHRAMGP EGRAAIGLGD GVVRISVGLE DTDD
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gacgagtctt cggtccgcac cccgaaggcg ctgcccgacg gcgtcagcca ggccaccgtc ggggtgcgcg gcgggatgtt gcggtcgggg ttcgaagaga ccgccgaggc gatgtacctg acgtccggat atgtctacgg ctcggcggcg gttgccgaga agtcgttcgc tggcgagctg gaccactatg tgtactcccg ctacggcaac ccaacggtgt cggtgttcga ggagcggctg cggctgatcg agggtgcccc ggcggcgttc gccaccgcca gtggcatggc cgcggtattc acctcgctgg gcgcgctgct gggtgccgga gaccgactgg ttgccgcgcg cagcctgttt ggctcgtgtt tcgtggtgtg cagcgagatc ctgccgcgct ggggggtgca gaccgtcttc gtcgacggtg acgacctctc gcaatgggag cgggcgcttt cggtacccac gcaggccgtg ttcttcgaga cgccgtccaa tcccatgcag tcgctggtgg atatcgctgc ggtgaccgag ctggcacatg ccgcgggtgc aaaagtggtg ctggacaacg tatttgccac accgctactg cagcagggct ttccgctggg ggtcgacgtg gtggtgtact cgggcaccaa gcacatcgac ggtcagggtc gggtgctggg cggggccata ctcggtgacc gggagtacat cgacggtccg gtgcaaaagc tgatgcgcca caccggtccg gcgatgagtg cgttcaacgc ctgggtactg ttgaaaggcc ttgagacgct ggctattcgg gtgcaacaca gcaatgcctc ggcgcagcgg atcgcggagt tcctcaacgg ccatccctcg gttcggtggg tgcgttaccc gtacctgccg tcgcacccac aatatgacct ggccaagcgt cagatgtccg gtggcggaac cgtcgttacc ttcgcactcg actgcccgga ggatgttgcc aaacagcggg ccttcgaggt gctcgacaag atgcggctga tcgacatctc caacaacctc ggcgacgcca aatcgcttgt cacccacccc gccaccacga cgcaccgggc gatgggcccg gagggccggg ccgcgatcgg gctcggtgac ggtgtggtcc gcatctcggt tgggttggaa gacaccgacg acc
Expected Protein Sequence
MAHHHHHHMT DESSVRTPKA LPDGVSQATV GVRGGMLRSG FEETAEAMYL TSGYVYGSAA VAEKSFAGEL DHYVYSRYGN PTVSVFEERL RLIEGAPAAF ATASGMAAVF TSLGALLGAG DRLVAARSLF GSCFVVCSEI LPRWGVQTVF VDGDDLSQWE RALSVPTQAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGFPLGVDV VVYSGTKHID GQGRVLGGAI LGDREYIDGP VQKLMRHTGP AMSAFNAWVL LKGLETLAIR VQHSNASAQR IAEFLNGHPS VRWVRYPYLP SHPQYDLAKR QMSGGGTVVT FALDCPEDVA KQRAFEVLDK MRLIDISNNL GDAKSLVTHP ATTTHRAMGP EGRAAIGLGD GVVRISVGLE DTDDLIADID RALS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acgagtcttc ggtccgcacc ccgaaggcgc tgcccgacgg cgtcagccag gccaccgtcg gggtgcgcgg cgggatgttg cggtcggggt tcgaagagac cgccgaggcg atgtacctga cgtccggata tgtctacggc tcggcggcgg ttgccgagaa gtcgttcgct ggcgagctgg accactatgt gtactcccgc tacggcaacc caacggtgtc ggtgttcgag gagcggctgc ggctgatcga gggtgccccg gcggcgttcg ccaccgccag tggcatggcc gcggtattca cctcgctggg cgcgctgctg ggtgccggag accgactggt tgccgcgcgc agcctgtttg gctcgtgttt cgtggtgtgc agcgagatcc tgccgcgctg gggggtgcag accgtcttcg tcgacggtga cgacctctcg caatgggagc gggcgctttc ggtacccacg caggccgtgt tcttcgagac gccgtccaat cccatgcagt cgctggtgga tatcgctgcg gtgaccgagc tggcacatgc cgcgggtgca aaagtggtgc tggacaacgt atttgccaca ccgctactgc agcagggctt tccgctgggg gtcgacgtgg tggtgtactc gggcaccaag cacatcgacg gtcagggtcg ggtgctgggc ggggccatac tcggtgaccg ggagtacatc gacggtccgg tgcaaaagct gatgcgccac accggtccgg cgatgagtgc gttcaacgcc tgggtactgt tgaaaggcct tgagacgctg gctattcggg tgcaacacag caatgcctcg gcgcagcgga tcgcggagtt cctcaacggc catccctcgg ttcggtgggt gcgttacccg tacctgccgt cgcacccaca atatgacctg gccaagcgtc agatgtccgg tggcggaacc gtcgttacct tcgcactcga ctgcccggag gatgttgcca aacagcgggc cttcgaggtg ctcgacaaga tgcggctgat cgacatctcc aacaacctcg gcgacgccaa atcgcttgtc acccaccccg ccaccacgac gcaccgggcg atgggcccgg agggccgggc cgcgatcggg ctcggtgacg gtgtggtccg catctcggtt gggttggaag acaccgacga cctgattgcc gatatcgatc gggcgttgag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for MytuD.01353.a.B1.PW27168
| PURIFICATION DATe: | 4/27/2010 |
| CONCENTRATION: | 26.91mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT DESSVRTPKA LPDGVSQATV GVRGGMLRSG FEETAEAMYL TSGYVYGSAA VAEKSFAGEL DHYVYSRYGN PTVSVFEERL RLIEGAPAAF ATASGMAAVF TSLGALLGAG DRLVAARSLF GSCFVVCSEI LPRWGVQTVF VDGDDLSQWE RALSVPTQAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGFPLGVDV VVYSGTKHID GQGRVLGGAI LGDREYIDGP VQKLMRHTGP AMSAFNAWVL LKGLETLAIR VQHSNASAQR IAEFLNGHPS VRWVRYPYLP SHPQYDLAKR QMSGGGTVVT FALDCPEDVA KQRAFEVLDK MRLIDISNNL GDAKSLVTHP ATTTHRAMGP EGRAAIGLGD GVVRISVGLE DTDD
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gacgagtctt cggtccgcac cccgaaggcg ctgcccgacg gcgtcagcca ggccaccgtc ggggtgcgcg gcgggatgtt gcggtcgggg ttcgaagaga ccgccgaggc gatgtacctg acgtccggat atgtctacgg ctcggcggcg gttgccgaga agtcgttcgc tggcgagctg gaccactatg tgtactcccg ctacggcaac ccaacggtgt cggtgttcga ggagcggctg cggctgatcg agggtgcccc ggcggcgttc gccaccgcca gtggcatggc cgcggtattc acctcgctgg gcgcgctgct gggtgccgga gaccgactgg ttgccgcgcg cagcctgttt ggctcgtgtt tcgtggtgtg cagcgagatc ctgccgcgct ggggggtgca gaccgtcttc gtcgacggtg acgacctctc gcaatgggag cgggcgcttt cggtacccac gcaggccgtg ttcttcgaga cgccgtccaa tcccatgcag tcgctggtgg atatcgctgc ggtgaccgag ctggcacatg ccgcgggtgc aaaagtggtg ctggacaacg tatttgccac accgctactg cagcagggct ttccgctggg ggtcgacgtg gtggtgtact cgggcaccaa gcacatcgac ggtcagggtc gggtgctggg cggggccata ctcggtgacc gggagtacat cgacggtccg gtgcaaaagc tgatgcgcca caccggtccg gcgatgagtg cgttcaacgc ctgggtactg ttgaaaggcc ttgagacgct ggctattcgg gtgcaacaca gcaatgcctc ggcgcagcgg atcgcggagt tcctcaacgg ccatccctcg gttcggtggg tgcgttaccc gtacctgccg tcgcacccac aatatgacct ggccaagcgt cagatgtccg gtggcggaac cgtcgttacc ttcgcactcg actgcccgga ggatgttgcc aaacagcggg ccttcgaggt gctcgacaag atgcggctga tcgacatctc caacaacctc ggcgacgcca aatcgcttgt cacccacccc gccaccacga cgcaccgggc gatgggcccg gagggccggg ccgcgatcgg gctcggtgac ggtgtggtcc gcatctcggt tgggttggaa gacaccgacg acc
Expressed Protein Sequence
MAHHHHHHMT DESSVRTPKA LPDGVSQATV GVRGGMLRSG FEETAEAMYL TSGYVYGSAA VAEKSFAGEL DHYVYSRYGN PTVSVFEERL RLIEGAPAAF ATASGMAAVF TSLGALLGAG DRLVAARSLF GSCFVVCSEI LPRWGVQTVF VDGDDLSQWE RALSVPTQAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGFPLGVDV VVYSGTKHID GQGRVLGGAI LGDREYIDGP VQKLMRHTGP AMSAFNAWVL LKGLETLAIR VQHSNASAQR IAEFLNGHPS VRWVRYPYLP SHPQYDLAKR QMSGGGTVVT FALDCPEDVA KQRAFEVLDK MRLIDISNNL GDAKSLVTHP ATTTHRAMGP EGRAAIGLGD GVVRISVGLE DTDDLIADID RALS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG ACGAGTCTTC GGTCCGCACC CCGAAGGCGC TGCCCGACGG CGTCAGCCAG GCCACCGTCG GGGTGCGCGG CGGGATGTTG CGGTCGGGGT TCGAAGAGAC CGCCGAGGCG ATGTACCTGA CGTCCGGATA TGTCTACGGC TCGGCGGCGG TTGCCGAGAA GTCGTTCGCT GGCGAGCTGG ACCACTATGT GTACTCCCGC TACGGCAACC CAACGGTGTC GGTGTTCGAG GAGCGGCTGC GGCTGATCGA GGGTGCCCCG GCGGCGTTCG CCACCGCCAG TGGCATGGCC GCGGTATTCA CCTCGCTGGG CGCGCTGCTG GGTGCCGGAG ACCGACTGGT TGCCGCGCGC AGCCTGTTTG GCTCGTGTTT CGTGGTGTGC AGCGAGATCC TGCCGCGCTG GGGGGTGCAG ACCGTCTTCG TCGACGGTGA CGACCTCTCG CAATGGGAGC GGGCGCTTTC GGTACCCACG CAGGCCGTGT TCTTCGAGAC GCCGTCCAAT CCCATGCAGT CGCTGGTGGA TATCGCTGCG GTGACCGAGC TGGCACATGC CGCGGGTGCA AAAGTGGTGC TGGACAACGT ATTTGCCACA CCGCTACTGC AGCAGGGCTT TCCGCTGGGG GTCGACGTGG TGGTGTACTC GGGCACCAAG CACATCGACG GTCAGGGTCG GGTGCTGGGC GGGGCCATAC TCGGTGACCG GGAGTACATC GACGGTCCGG TGCAAAAGCT GATGCGCCAC ACCGGTCCGG CGATGAGTGC GTTCAACGCC TGGGTACTGT TGAAAGGCCT TGAGACGCTG GCTATTCGGG TGCAACACAG CAATGCCTCG GCGCAGCGGA TCGCGGAGTT CCTCAACGGC CATCCCTCGG TTCGGTGGGT GCGTTACCCG TACCTGCCGT CGCACCCACA ATATGACCTG GCCAAGCGTC AGATGTCCGG TGGCGGAACC GTCGTTACCT TCGCACTCGA CTGCCCGGAG GATGTTGCCA AACAGCGGGC CTTCGAGGTG CTCGACAAGA TGCGGCTGAT CGACATCTCC AACAACCTCG GCGACGCCAA ATCGCTTGTC ACCCACCCCG CCACCACGAC GCACCGGGCG ATGGGCCCGG AGGGCCGGGC CGCGATCGGG CTCGGTGACG GTGTGGTCCG CATCTCGGTT GGGTTGGAAG ACACCGACGA CCTGATTGCC GATATCGATC GGGCGTTGAG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT