SSGCID: Target Page

MyavA.01353.a

O-succinylhomoserine sulfhydrylase

CENTER ID:	MyavA.01353.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01353.a.A1.GE29114	Full length( MyavA.01353.a )		1	408

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01353.a.A1.PS00858	Full length( MyavA.01353.a )		1	408

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4692
RefSeq:	YP_883900.1
UniProt:	A0A0H2ZYF1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQAGDDSVR TPPALPDGVS QATIGVRGGL LRSGFDETAE ALYLTSGYVY ESAAVAEQSF TGELDHFVYS RYGNPTVTMF EERLRLLEGA PAAFATASGM AAVFTSLGAL LAAGDRLVAA RSLFGSCFVV CNEILPRWGV QTVFVDGDDL AQWEEALSVP TAAVFFETPS NPMQSLVDIA AVTELAHAAG AKVVLDNVFA TPLLQQGIPL GVDVVVYSGT KHIDGQGRVL GGAILGDRDY IDGPVQKLMR HTGPAMSAFN AWVLLKGLET MAIRVEHSNS SAHRIAEFLE THPAVRWVRY PYLPSHPQYD LAKRQMSGGG TVITFALDCP DDKAKQRAFE VLDKLTLIDI SNNLGDAKSL VTHPATTTHR AMGPEGRAAI GLGDGVVRIS VGLEGTDDLI ADIDRALG

NT Sequence

ATGAGTCAGG CCGGCGACGA TTCGGTCCGC ACGCCCCCCG CGCTGCCCGA CGGCGTCAGC CAGGCGACCA TCGGCGTGCG CGGCGGACTG CTGCGTTCGG GATTCGACGA GACCGCCGAG GCGCTGTATC TGACCTCCGG CTACGTCTAC GAGTCGGCCG CCGTTGCCGA GCAGTCGTTC ACCGGCGAAC TGGACCATTT CGTCTACTCC CGCTACGGCA ACCCCACCGT GACCATGTTC GAGGAGCGGC TGCGCCTGCT CGAAGGCGCG CCGGCGGCGT TCGCCACCGC GAGCGGCATG GCGGCGGTGT TCACCTCGCT GGGCGCGCTG CTGGCCGCGG GCGACCGGCT GGTCGCCGCG CGCAGCCTGT TCGGCTCCTG CTTCGTGGTG TGCAACGAGA TCCTGCCCCG CTGGGGCGTG CAGACGGTGT TCGTCGACGG CGACGACCTC GCGCAGTGGG AGGAGGCGTT GTCGGTGCCG ACGGCGGCGG TGTTCTTCGA GACGCCGTCG AACCCGATGC AGTCGCTGGT GGACATCGCC GCGGTCACCG AGCTCGCCCA CGCCGCGGGC GCAAAGGTGG TGCTGGACAA CGTTTTCGCC ACCCCGTTGC TGCAGCAGGG CATCCCGCTC GGGGTGGACG TGGTGGTGTA CTCGGGCACC AAACACATCG ACGGCCAGGG CCGGGTGCTG GGCGGGGCCA TCCTCGGGGA CAGGGACTAC ATCGACGGGC CGGTGCAGAA GCTGATGCGG CACACCGGTC CGGCGATGAG CGCGTTCAAC GCCTGGGTGC TGCTGAAAGG CCTTGAGACC ATGGCCATCC GGGTGGAACA CAGCAATTCC TCGGCGCACC GGATCGCCGA ATTCCTGGAG ACCCATCCCG CGGTGCGCTG GGTCCGCTAC CCGTATCTGC CGTCCCACCC GCAGTACGAC CTGGCCAAGC GCCAGATGTC CGGCGGCGGA ACGGTGATCA CCTTCGCGCT GGACTGCCCC GACGACAAGG CCAAGCAGCG GGCCTTCGAG GTGCTGGACA AGCTGACGCT GATCGACATC TCCAACAACC TCGGTGACGC CAAATCCCTT GTCACACATC CCGCTACCAC CACCCACCGG GCGATGGGCC CGGAGGGCCG CGCGGCGATC GGGCTGGGCG ACGGCGTGGT CCGCATCTCG GTCGGGCTGG AAGGCACCGA CGACTTGATC GCCGACATCG ACCGCGCGCT GGGT

Details for MyavA.01353.a.A1.GE29114

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	22
PERCENT COVERAGE:	64

Validated AA Sequence

MGLQEFGDPV RRGIAVFHPD GHGLKAFQQH PGVERAHRRT GVPHQLLHRP VDVVPVPEDG PAQHPALAVD VFGARVHHHV HPERDALLQQ RGGENVVQHH LCARGVGELG DRGDVHQRLH RVRRRLEEHR RRRHRQRLLP LREVVAVDEH RLHAPAGQDL VAHHEAGAEQ AARGDQPVAR GQQRAQRGEH RRHAARGGER RRRAFEQAQP LLEHGHGGVA VAGVDEMVQF AGERLLGNGG RLVDVAGGQI QRLGGLVESR TQQSAAHADG RLADAVGQ

Validated NT Sequence

gcaccgcggg atgggtctcc aggaattcgg cgatccggtg cgccgaggaa ttgctgtgtt ccacccggat ggccatggtc tcaaggcctt tcagcagcac ccaggcgttg aacgcgctca tcgccggacc ggtgtgccgc atcagcttct gcaccggccc gtcgatgtag tccctgtccc cgaggatggc cccgcccagc acccggccct ggccgtcgat gtgtttggtg cccgagtaca ccaccacgtc caccccgagc gggatgccct gctgcagcaa cggggtggcg aaaacgttgt ccagcaccac ctttgcgccc gcggcgtggg cgagctcggt gaccgcggcg atgtccacca gcgactgcat cgggttcgac ggcgtctcga agaacaccgc cgccgtcggc accgacaacg cctcctccca ctgcgcgagg tcgtcgccgt cgacgaacac cgtctgcacg ccccagcggg gcaggatctc gttgcacacc acgaagcagg agccgaacag gctgcgcgcg gcgaccagcc ggtcgcccgc ggccagcagc gcgcccagcg aggtgaacac cgccgccatg ccgctcgcgg tggcgaacgc cgccggcgcg ccttcgagca ggcgcagccg ctcctcgaac atggtcacgg tggggttgcc gtagcgggag tagacgaaat ggtccagttc gccggtgaac gactgctcgg caacggcggc cgactcgtag acgtagccgg aggtcagata cagcgcctcg gcggtctcgt cgaatcccga acgcagcagt ccgccgcgca cgccgatggt cgcctggctg acgccgtcgg gcagcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSQAGDDSV RTPPALPDGV SQATIGVRGG LLRSGFDETA EALYLTSGYV YESAAVAEQS FTGELDHFVY SRYGNPTVTM FEERLRLLEG APAAFATASG MAAVFTSLGA LLAAGDRLVA ARSLFGSCFV VCNEILPRWG VQTVFVDGDD LAQWEEALSV PTAAVFFETP SNPMQSLVDI AAVTELAHAA GAKVVLDNVF ATPLLQQGIP LGVDVVVYSG TKHIDGQGRV LGGAILGDRD YIDGPVQKLM RHTGPAMSAF NAWVLLKGLE TMAIRVEHSN SSAHRIAEFL ETHPAVRWVR YPYLPSHPQY DLAKRQMSGG GTVITFALDC PDDKAKQRAF EVLDKLTLID ISNNLGDAKS LVTHPATTTH RAMGPEGRAA IGLGDGVVRI SVGLEGTDDL IADIDRALG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtcaggcc ggcgacgatt cggtccgcac gccccccgcg ctgcccgacg gcgtcagcca ggcgaccatc ggcgtgcgcg gcggactgct gcgttcggga ttcgacgaga ccgccgaggc gctgtatctg acctccggct acgtctacga gtcggccgcc gttgccgagc agtcgttcac cggcgaactg gaccatttcg tctactcccg ctacggcaac cccaccgtga ccatgttcga ggagcggctg cgcctgctcg aaggcgcgcc ggcggcgttc gccaccgcga gcggcatggc ggcggtgttc acctcgctgg gcgcgctgct ggccgcgggc gaccggctgg tcgccgcgcg cagcctgttc ggctcctgct tcgtggtgtg caacgagatc ctgccccgct ggggcgtgca gacggtgttc gtcgacggcg acgacctcgc gcagtgggag gaggcgttgt cggtgccgac ggcggcggtg ttcttcgaga cgccgtcgaa cccgatgcag tcgctggtgg acatcgccgc ggtcaccgag ctcgcccacg ccgcgggcgc aaaggtggtg ctggacaacg ttttcgccac cccgttgctg cagcagggca tcccgctcgg ggtggacgtg gtggtgtact cgggcaccaa acacatcgac ggccagggcc gggtgctggg cggggccatc ctcggggaca gggactacat cgacgggccg gtgcagaagc tgatgcggca caccggtccg gcgatgagcg cgttcaacgc ctgggtgctg ctgaaaggcc ttgagaccat ggccatccgg gtggaacaca gcaattcctc ggcgcaccgg atcgccgaat tcctggagac ccatcccgcg gtgcgctggg tccgctaccc gtatctgccg tcccacccgc agtacgacct ggccaagcgc cagatgtccg gcggcggaac ggtgatcacc ttcgcgctgg actgccccga cgacaaggcc aagcagcggg ccttcgaggt gctggacaag ctgacgctga tcgacatctc caacaacctc ggtgacgcca aatcccttgt cacacatccc gctaccacca cccaccgggc gatgggcccg gagggccgcg cggcgatcgg gctgggcgac ggcgtggtcc gcatctcggt cgggctggaa ggcaccgacg acttgatcgc cgacatcgac cgcgcgctgg gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MyavA.01353.a.A1.PS00858

PURIFICATION DATe:	11/4/2010
CONCENTRATION:	13.7mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	21
VIAL VOLUME:	200µl
PERCENT IDENTITY:	22
PERCENT COVERAGE:	64

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTCAGGCC GGCGACGATT CGGTCCGCAC GCCCCCCGCG CTGCCCGACG GCGTCAGCCA GGCGACCATC GGCGTGCGCG GCGGACTGCT GCGTTCGGGA TTCGACGAGA CCGCCGAGGC GCTGTATCTG ACCTCCGGCT ACGTCTACGA GTCGGCCGCC GTTGCCGAGC AGTCGTTCAC CGGCGAACTG GACCATTTCG TCTACTCCCG CTACGGCAAC CCCACCGTGA CCATGTTCGA GGAGCGGCTG CGCCTGCTCG AAGGCGCGCC GGCGGCGTTC GCCACCGCGA GCGGCATGGC GGCGGTGTTC ACCTCGCTGG GCGCGCTGCT GGCCGCGGGC GACCGGCTGG TCGCCGCGCG CAGCCTGTTC GGCTCCTGCT TCGTGGTGTG CAACGAGATC CTGCCCCGCT GGGGCGTGCA GACGGTGTTC GTCGACGGCG ACGACCTCGC GCAGTGGGAG GAGGCGTTGT CGGTGCCGAC GGCGGCGGTG TTCTTCGAGA CGCCGTCGAA CCCGATGCAG TCGCTGGTGG ACATCGCCGC GGTCACCGAG CTCGCCCACG CCGCGGGCGC AAAGGTGGTG CTGGACAACG TTTTCGCCAC CCCGTTGCTG CAGCAGGGCA TCCCGCTCGG GGTGGACGTG GTGGTGTACT CGGGCACCAA ACACATCGAC GGCCAGGGCC GGGTGCTGGG CGGGGCCATC CTCGGGGACA GGGACTACAT CGACGGGCCG GTGCAGAAGC TGATGCGGCA CACCGGTCCG GCGATGAGCG CGTTCAACGC CTGGGTGCTG CTGAAAGGCC TTGAGACCAT GGCCATCCGG GTGGAACACA GCAATTCCTC GGCGCACCGG ATCGCCGAAT TCCTGGAGAC CCATCCCGCG GTGCGCTGGG TCCGCTACCC GTATCTGCCG TCCCACCCGC AGTACGACCT GGCCAAGCGC CAGATGTCCG GCGGCGGAAC GGTGATCACC TTCGCGCTGG ACTGCCCCGA CGACAAGGCC AAGCAGCGGG CCTTCGAGGT GCTGGACAAG CTGACGCTGA TCGACATCTC CAACAACCTC GGTGACGCCA AATCCCTTGT CACACATCCC GCTACCACCA CCCACCGGGC GATGGGCCCG GAGGGCCGCG CGGCGATCGG GCTGGGCGAC GGCGTGGTCC GCATCTCGGT CGGGCTGGAA GGCACCGACG ACTTGATCGC CGACATCGAC CGCGCGCTGG GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA