MymaA.01353.a

O-succinylhomoserine sulfhydrylase, MetZ

CENTER ID: MymaA.01353.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01353.a.A1.GE30172 Full length( MymaA.01353.a ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.738
RefSeq: YP_001849002.1
UniProt: B2HPX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDDRSVPEA ERPKNPRKLP DGVSQATIGV RGGMLRSGFD ETAEALYLTS GYVYESAAMA EQSFAGELDH FLYSRYGNPT VAMFEERLRL IEAAPAVFAT ASGMAAVFTA LGALLGAGDR LVASRSLFGS CFVVCNEILP RWGVETVFVD GDDLEQWEQA LSVPTEAVFF ETPSNPMQSL VDIAAVTELA HAAGAKVVLD NVFATPLLQQ GIPLGVDVVV YSGTKHIDGQ GRVLGGAILG DQDYIDGPVQ KLMRHTGPAL SAFNAWILLK GLETLAIRVQ HSNSSALRIA EFLQGHPAVR WVRYPYLPSH PQYDLAKRQM SGGGTVVTFA LDAPESAAKR RAFEVLDKLR LIDISNNLGD TKSLITHPAT TTHRAMGPEG RAAIGLGDAV VRISVGLEGA EDLIADIDQA LG
NT Sequence
GTGACCGACG ATCGGTCGGT GCCAGAGGCC GAGCGGCCCA AGAATCCCCG GAAGTTGCCC GATGGCGTCA GCCAGGCCAC CATCGGCGTG CGCGGGGGCA TGTTGCGGTC TGGATTCGAC GAGACCGCCG AAGCGCTGTA TCTGACCTCG GGCTACGTAT ACGAGTCGGC CGCGATGGCC GAGCAGTCGT TCGCCGGCGA ACTCGACCAC TTTTTGTACT CGCGCTACGG CAATCCGACC GTGGCCATGT TCGAAGAACG CCTTCGGCTG ATCGAAGCAG CTCCGGCCGT GTTCGCCACG GCCAGCGGTA TGGCAGCGGT ATTCACCGCT CTGGGTGCGC TGCTGGGTGC GGGCGACCGG CTGGTGGCGT CTCGCAGTCT GTTCGGGTCG TGCTTTGTGG TGTGCAACGA GATCCTGCCG CGCTGGGGCG TGGAAACGGT GTTCGTTGAC GGTGACGACC TTGAGCAGTG GGAGCAAGCG CTATCGGTGC CCACCGAGGC GGTGTTCTTC GAAACGCCGT CCAACCCGAT GCAATCGCTG GTCGACATCG CCGCGGTGAC CGAGCTGGCG CATGCCGCGG GCGCAAAAGT TGTGCTGGAC AACGTTTTTG CTACTCCGCT GCTGCAGCAG GGAATTCCGC TCGGGGTCGA TGTGGTGGTG TACTCGGGTA CCAAGCACAT CGACGGTCAG GGCAGGGTGC TGGGCGGTGC CATTCTTGGC GACCAGGACT ACATCGATGG GCCGGTGCAG AAGCTGATGC GCCACACCGG TCCGGCGCTC AGCGCTTTCA ACGCCTGGAT TCTACTCAAA GGTCTTGAGA CGCTTGCGAT TCGGGTTCAG CATAGTAATT CCTCGGCGCT GCGCATCGCC GAGTTTCTGC AGGGCCACCC CGCGGTGCGA TGGGTGCGTT ATCCGTATCT GCCCTCACAT CCGCAGTATG ACCTGGCCAA GCGTCAGATG TCTGGCGGCG GAACCGTGGT GACGTTTGCA CTCGACGCCC CGGAAAGTGC GGCCAAGCGG CGCGCTTTCG AAGTGCTCGA CAAGCTGAGA TTGATTGACA TCTCCAATAA CCTCGGCGAC ACCAAATCGT TGATCACCCA CCCGGCAACC ACGACGCACC GGGCGATGGG CCCGGAGGGT CGCGCCGCGA TCGGGCTCGG TGACGCAGTG GTGCGCATTT CGGTCGGGCT GGAGGGCGCC GAGGATCTGA TCGCCGATAT TGATCAGGCA CTCGGC
Details for MymaA.01353.a.A1.GE30172
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 67
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDRSVP EAERPKNPRK LPDGVSQATI GVRGGMLRSG FDETAEALYL TSGYVYESAA MAEQSFAGEL DHFLYSRYGN PTVAMFEERL RLIEAAPAVF ATASGMAAVF TALGALLGAG DRLVASRSLF GSCFVVCNEI LPRWGVETVF VDGDDLEQWE QALSVPTEAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGXSARGRC GGVLGYQAHX RXXXXWAVXF LATRTTSWAX AXAXAPXRSX AQRXQRLXST XXLXXLAXRX QHXXXXXCAS XXXXPXXXWX XXXXLXXXXX XTXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgac cgacgatcgg tcggtgccag aggccgagcg gcccaagaat ccccggaagt tgcccgatgg cgtcagccag gccaccatcg gcgtgcgcgg gggcatgttg cggtctggat tcgacgagac cgccgaagcg ctgtatctga cctcgggcta cgtatacgag tcggccgcga tggccgagca gtcgttcgcc ggcgaactcg accacttttt gtactcgcgc tacggcaatc cgaccgtggc catgttcgaa gaacgccttc ggctgatcga agcagctccg gccgtgttcg ccacggccag cggtatggca gcggtattca ccgctctggg tgcgctgctg ggtgcgggcg accggctggt ggcgtctcgc agtctgttcg ggtcgtgctt tgtggtgtgc aacgagatcc tgccgcgctg gggcgtggaa acggtgttcg ttgacggtga cgaccttgag cagtgggagc aagcgctatc ggtgcccacc gaggcggtgt tcttcgaaac gccgtccaac ccgatgcaat cgctggtcga catcgccgcg gtgaccgagc tggcgcatgc cgcgggcgca aaagttgtgc tggacaacgt ttttgctact ccgctgctgc agcagggaan ttccgctcgg ggtcgatgtg gtggtgtact cgggtaccaa gcacatcnac gtcnggnnnn nngctgggcg gtgncattct tggcgaccag gactacatca tgggccngtg cnnaagctga ngcgccacan cggtccnnng ctcagcgctn tcaacgcctg gnttctactc annnncttga nangcttgcn antcgnnntc agcatantaa ntnctnnnnc tgcgcatcnn cnanntnctn nccccnnnnn ngatgggngc nntntcngta nctncnnnnn cnnncnnngn agacnnnnna nnntcnnann ncnnnngnnn nnngnnacnn tnnnnnnnga ncnnnnangn gnnnnnnngn nnncntnnnn nnnnnnnnnn ntnnnnnnan nnnnnnnnnn nnnnnnnnnc nannnnnnnn nnnnnang
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDRSVP EAERPKNPRK LPDGVSQATI GVRGGMLRSG FDETAEALYL TSGYVYESAA MAEQSFAGEL DHFLYSRYGN PTVAMFEERL RLIEAAPAVF ATASGMAAVF TALGALLGAG DRLVASRSLF GSCFVVCNEI LPRWGVETVF VDGDDLEQWE QALSVPTEAV FFETPSNPMQ SLVDIAAVTE LAHAAGAKVV LDNVFATPLL QQGIPLGVDV VVYSGTKHID GQGRVLGGAI LGDQDYIDGP VQKLMRHTGP ALSAFNAWIL LKGLETLAIR VQHSNSSALR IAEFLQGHPA VRWVRYPYLP SHPQYDLAKR QMSGGGTVVT FALDAPESAA KRRAFEVLDK LRLIDISNNL GDTKSLITHP ATTTHRAMGP EGRAAIGLGD AVVRISVGLE GAEDLIADID QALG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgac gatcggtcgg tgccagaggc cgagcggccc aagaatcccc ggaagttgcc cgatggcgtc agccaggcca ccatcggcgt gcgcgggggc atgttgcggt ctggattcga cgagaccgcc gaagcgctgt atctgacctc gggctacgta tacgagtcgg ccgcgatggc cgagcagtcg ttcgccggcg aactcgacca ctttttgtac tcgcgctacg gcaatccgac cgtggccatg ttcgaagaac gccttcggct gatcgaagca gctccggccg tgttcgccac ggccagcggt atggcagcgg tattcaccgc tctgggtgcg ctgctgggtg cgggcgaccg gctggtggcg tctcgcagtc tgttcgggtc gtgctttgtg gtgtgcaacg agatcctgcc gcgctggggc gtggaaacgg tgttcgttga cggtgacgac cttgagcagt gggagcaagc gctatcggtg cccaccgagg cggtgttctt cgaaacgccg tccaacccga tgcaatcgct ggtcgacatc gccgcggtga ccgagctggc gcatgccgcg ggcgcaaaag ttgtgctgga caacgttttt gctactccgc tgctgcagca gggaattccg ctcggggtcg atgtggtggt gtactcgggt accaagcaca tcgacggtca gggcagggtg ctgggcggtg ccattcttgg cgaccaggac tacatcgatg ggccggtgca gaagctgatg cgccacaccg gtccggcgct cagcgctttc aacgcctgga ttctactcaa aggtcttgag acgcttgcga ttcgggttca gcatagtaat tcctcggcgc tgcgcatcgc cgagtttctg cagggccacc ccgcggtgcg atgggtgcgt tatccgtatc tgccctcaca tccgcagtat gacctggcca agcgtcagat gtctggcggc ggaaccgtgg tgacgtttgc actcgacgcc ccggaaagtg cggccaagcg gcgcgctttc gaagtgctcg acaagctgag attgattgac atctccaata acctcggcga caccaaatcg ttgatcaccc acccggcaac cacgacgcac cgggcgatgg gcccggaggg tcgcgccgcg atcgggctcg gtgacgcagt ggtgcgcatt tcggtcgggc tggagggcgc cgaggatctg atcgccgata ttgatcaggc actcggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa