MyleA.01353.a

Putative o-succinylhomoserine sulfhydrylase

CENTER ID: MyleA.01353.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01353.a.A1.GE29264 Full length( MyleA.01353.a ) 1 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.435
RefSeq: YP_002502949.1
UniProt: A0A0H3MTX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDDRSVRTP KALPDGVTAA TVGVRGGLLR SRFEETAETM YLTSGYVYES AVVAEKSFTG ELDHFVYSRY GNPTVTMFEE RLRLIEGAPA AFVTASGMAA VFTSLGALLA SGDRLVAARS LFGSCFVVCN EILPRWGVET VFVDGEDLVQ WEQALSVPTQ AVFFETPSNP MQSLVDIAAV TELAHAAGAK VVLDNAFATP LLQHGLPLGV DVVIYSGTKH IDGQGRVLGG AILGDRDYID GPVQKLMRHT GPAMSAFNAW ILLKGLETLA IRVDHCNSSA HRIAEFLEKH PAVSWVRYPF LVSHPQYDLA KRQMSGGGTV VTFALNSPED AAKQRAFEVL DRLRLIDISN NFGDVKSLIT HPATTTHRAM GPEGRAAIGL GDGVVRISIG LEDAADLIAD IDQALS
NT Sequence
ATGACCGACG ACCGCTCTGT TCGCACGCCT AAGGCTTTGC CGGACGGCGT CACCGCGGCA ACCGTGGGCG TCCGTGGTGG CCTGTTACGG TCCCGGTTCG AAGAAACCGC CGAGACGATG TACTTGACGT CTGGTTACGT CTACGAGTCA GCGGTCGTTG CAGAAAAGTC GTTCACCGGC GAGCTGGACC ACTTTGTGTA TTCGCGTTAC GGCAACCCTA CCGTGACGAT GTTCGAGGAA CGGTTACGCC TGATCGAGGG AGCCCCTGCG GCGTTCGTCA CCGCGAGCGG CATGGCTGCG GTTTTCACTT CGCTAGGTGC GTTGTTGGCC TCCGGCGACC GGTTGGTGGC CGCGCGCAGT CTGTTTGGGT CATGTTTCGT GGTGTGCAAT GAAATTCTAC CGCGGTGGGG TGTCGAGACC GTCTTCGTCG ACGGCGAAGA CCTTGTGCAA TGGGAGCAGG CGCTTTCGGT TCCAACTCAA GCGGTGTTCT TTGAGACGCC CTCGAATCCG ATGCAGTCGC TGGTAGATAT CGCGGCCGTG ACTGAGTTGG CTCACGCGGC TGGAGCAAAA GTTGTTCTGG ACAATGCCTT TGCTACACCA CTACTGCAGC ACGGTTTACC GCTCGGGGTT GACGTGGTAA TCTATTCAGG TACTAAACAC ATCGACGGCC AGGGGCGGGT GTTGGGCGGT GCCATTCTGG GTGACAGGGA TTACATCGAC GGTCCTGTGC AAAAGCTGAT GCGCCACACT GGCCCAGCAA TGAGCGCTTT CAATGCCTGG ATATTGCTGA AAGGTCTTGA GACACTTGCG ATTAGGGTCG ATCACTGTAA TTCCTCGGCG CACCGTATTG CAGAGTTCTT GGAGAAGCAC CCGGCGGTGA GTTGGGTGCG CTATCCATTT TTGGTGTCGC ATCCGCAGTA CGACCTGGCG AAGCGCCAAA TGTCTGGTGG TGGAACGGTG GTGACCTTTG CGCTCAATTC TCCGGAGGAC GCCGCTAAGC AACGAGCCTT CGAAGTATTG GACAGGCTGC GGCTGATCGA TATTTCTAAC AACTTCGGTG ACGTTAAATC GCTTATTACG CACCCGGCGA CTACCACTCA CCGCGCGATG GGCCCGGAGG GCCGTGCTGC GATCGGGCTC GGTGACGGTG TGGTACGCAT CTCCATTGGC CTCGAAGACG CCGCCGATCT GATCGCTGAT ATTGATCAGG CCCTGAGC
Details for MyleA.01353.a.A1.GE29264
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 92
PERCENT COVERAGE: 85
Validated AA Sequence
MYLTSGYVYE SAVVAEKSFT GELDHFVYSR YGNPTVTMFE ERLRLIEGAP AAFVTASGMA AVFTSLGALL ASGDRLVAAR SLFGSCFVVC NEILPRWGVE TVFVDGEXLV QWEQALSVPT QAVFFETPSN PMQSLVDIAA VTELAHAAGA KVVLDNAFAT PLLQHGLPLG VDVVIYSGTK HXDGQGRVLG GAIXGDRDYI DGPVQKXMRH TGPAMSAFNA WILLKGLETL AIRVDHCNSX AHXIAEFLEK XPAVSWVRXX FLVSHPQYDL XKXQXSGGGT VVTFALNSPE DAAKQRAFEV LDRLRLXDIS NNXGDVKSLI THPXXTTXXX MGPEGRAAIG XGXXVVRISX GLEDAADXIA DXXQ
Validated NT Sequence
tctgttcgca cgcctaaggc tttgccggac ggcgtcaccg cggcaaccgn gggcgtccgt ggtggcctgt tacggtcccg gttcgaagaa accgccgaga cgatgtactt gacgtctggt tacgtctacg agtcagcggt cgttgcagaa aagtcgttca ccggcgagct ggaccacttt gtgtattcgc gttacggcaa ccctaccgtg acgatgttcg aggaacggtt acgcctgatc gagggagccc ctgcggcgtt cgtcaccgcg agcggcatgg ctgcggtttt cacttcgcta ggtgcgttgt tggcctccgg cgaccggttg gtggccgcgc gcagtctgtt tgggtcatgt ttcgtggtgt gcaatgaaat tctaccgcgg tggggtgtcg agaccgtctt cgtcgacggc gaanaccttg tgcaatggga gcaggcgctt tcggttccaa ctcaagcggt gttctttgag acgccctcna atccnatgca gtcgctggta gatatcgcgg ccgtgactga gttggctcac gcggctggag caaaagttgt tctggacaat gcctttgcta caccactact gcagcacggt ttaccgctcg gggttgacgt ggtaatctat tcaggtacta aacacatnga cggccagggg cgggtgttgg gcggtgccat tcnnggtgac agggattaca tcgacggtcc tgtgcaaaag ntgatgcgcc acactggncc agcaatgagc gctttcaatg cctggatatt gctgaaaggt cttgagacac ttgcgattag ggtcgatcac tgtaattcct nggcgcaccn tattgcagag ttcttggaga agcncccggc ggtgagttgg gtgcgctann catttttggt gtcgcatccg cagtacgacc tggngaagcn ccaaanntct ggtggtggaa cggtggtgac ctttgcgctc aattctccgg aggacgccgc taagcaacga gccttcgaag tattggacag gctgcggctg atngatattt ctaacaactt nggngacgtt aaatcgctta ttacgcaccc gncgnntacc actcnncncn cgatgggccc ggagggccgt gctgcgatcg gnntcggngn cgnngtggta cgcatctcca tnggcctnga agacgccgcc gatntnatcg ctgatatnna tcaggc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDDRSVRT PKALPDGVTA ATVGVRGGLL RSRFEETAET MYLTSGYVYE SAVVAEKSFT GELDHFVYSR YGNPTVTMFE ERLRLIEGAP AAFVTASGMA AVFTSLGALL ASGDRLVAAR SLFGSCFVVC NEILPRWGVE TVFVDGEDLV QWEQALSVPT QAVFFETPSN PMQSLVDIAA VTELAHAAGA KVVLDNAFAT PLLQHGLPLG VDVVIYSGTK HIDGQGRVLG GAILGDRDYI DGPVQKLMRH TGPAMSAFNA WILLKGLETL AIRVDHCNSS AHRIAEFLEK HPAVSWVRYP FLVSHPQYDL AKRQMSGGGT VVTFALNSPE DAAKQRAFEV LDRLRLIDIS NNFGDVKSLI THPATTTHRA MGPEGRAAIG LGDGVVRISI GLEDAADLIA DIDQALS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgacgac cgctctgttc gcacgcctaa ggctttgccg gacggcgtca ccgcggcaac cgtgggcgtc cgtggtggcc tgttacggtc ccggttcgaa gaaaccgccg agacgatgta cttgacgtct ggttacgtct acgagtcagc ggtcgttgca gaaaagtcgt tcaccggcga gctggaccac tttgtgtatt cgcgttacgg caaccctacc gtgacgatgt tcgaggaacg gttacgcctg atcgagggag cccctgcggc gttcgtcacc gcgagcggca tggctgcggt tttcacttcg ctaggtgcgt tgttggcctc cggcgaccgg ttggtggccg cgcgcagtct gtttgggtca tgtttcgtgg tgtgcaatga aattctaccg cggtggggtg tcgagaccgt cttcgtcgac ggcgaagacc ttgtgcaatg ggagcaggcg ctttcggttc caactcaagc ggtgttcttt gagacgccct cgaatccgat gcagtcgctg gtagatatcg cggccgtgac tgagttggct cacgcggctg gagcaaaagt tgttctggac aatgcctttg ctacaccact actgcagcac ggtttaccgc tcggggttga cgtggtaatc tattcaggta ctaaacacat cgacggccag gggcgggtgt tgggcggtgc cattctgggt gacagggatt acatcgacgg tcctgtgcaa aagctgatgc gccacactgg cccagcaatg agcgctttca atgcctggat attgctgaaa ggtcttgaga cacttgcgat tagggtcgat cactgtaatt cctcggcgca ccgtattgca gagttcttgg agaagcaccc ggcggtgagt tgggtgcgct atccattttt ggtgtcgcat ccgcagtacg acctggcgaa gcgccaaatg tctggtggtg gaacggtggt gacctttgcg ctcaattctc cggaggacgc cgctaagcaa cgagccttcg aagtattgga caggctgcgg ctgatcgata tttctaacaa cttcggtgac gttaaatcgc ttattacgca cccggcgact accactcacc gcgcgatggg cccggagggc cgtgctgcga tcgggctcgg tgacggtgtg gtacgcatct ccattggcct cgaagacgcc gccgatctga tcgctgatat tgatcaggcc ctgagcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa