MytuD.01212.a

Malate dehydrogenase

CENTER ID: MytuD.01212.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01212.a.A1.GE25949 Full length( MytuD.01212.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01212.a.A1.PS00155 Full length( MytuD.01212.a ) 1 329

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1389
RefSeq: NP_215756.1
TubercuList: Rv1240
MTB Network Portal: Rv1240
UniProt: P9WK13

Orthologues for Rv1240

Species % Identity % Coverage Clones Proteins Structures
marinum 94 100 1 0 0
ulcerans 93 100 0 0 0
paratuberculosis 92 100 1 0 0
avium 91 100 1 1 0
leprae 89 100 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALQA LEGVVMELDD CAFPLLSGVE IGSDPQKIFD GVSLALLVGA RPRGAGMERS DLLEANGAIF TAQGKALNAV AADDVRVGVT GNPANTNALI AMTNAPDIPR ERFSALTRLD HNRAISQLAA KTGAAVTDIK KMTIWGNHSA TQYPDLFHAE VAGKNAAEVV NDQAWIEDEF IPTVAKRGAA IIDARGASSA ASAASATIDA ARDWLLGTPA DDWVSMAVVS DGSYGVPEGL ISSFPVTTKG GNWTIVSGLE IDEFSRGRID KSTAELADER SAVTELGLI
NT Sequence
GTGAGCGCTA GTCCTCTCAA GGTCGCCGTT ACCGGCGCCG CCGGCCAAAT CGGCTACAGC CTGTTGTTCC GCCTGGCCAG CGGCTCTTTG CTGGGCCCTG ACCGTCCGAT CGAGCTGCGG CTGCTCGAGA TCGAGCCGGC ACTGCAGGCG CTCGAGGGTG TGGTGATGGA ACTCGACGAC TGCGCTTTCC CGCTGTTGTC CGGGGTGGAG ATCGGTTCCG ATCCCCAGAA GATCTTCGAT GGTGTGAGCC TGGCCCTGCT GGTCGGAGCC CGCCCCCGGG GCGCGGGCAT GGAGCGAAGT GACCTGCTGG AGGCCAACGG CGCGATCTTC ACCGCTCAGG GCAAAGCCCT CAACGCTGTC GCCGCGGATG ACGTTCGCGT CGGGGTGACC GGCAACCCCG CCAACACCAA CGCGCTGATC GCGATGACCA ATGCGCCCGA CATTCCCCGC GAGCGGTTCT CGGCGCTCAC CCGGCTGGAC CACAATCGGG CGATCTCGCA GCTGGCCGCC AAGACCGGCG CGGCGGTCAC CGACATCAAG AAGATGACGA TCTGGGGCAA TCACTCGGCC ACCCAGTACC CCGACCTGTT CCACGCGGAG GTCGCCGGAA AGAACGCGGC CGAAGTGGTC AACGACCAGG CCTGGATCGA GGATGAATTC ATCCCGACGG TCGCCAAGCG CGGTGCGGCG ATCATCGATG CGCGCGGCGC GTCGTCGGCC GCCTCGGCCG CGTCGGCAAC CATCGACGCT GCCCGGGACT GGTTGCTGGG GACGCCGGCG GACGATTGGG TCTCGATGGC CGTCGTCTCC GACGGGTCCT ACGGGGTGCC GGAGGGCTTG ATCTCCTCGT TTCCGGTCAC CACCAAGGGC GGCAACTGGA CGATCGTGAG CGGCTTGGAG ATCGACGAGT TCTCCCGCGG CCGGATCGAC AAGTCAACCG CCGAGTTGGC TGACGAGCGC AGCGCGGTCA CCGAGCTCGG CCTGATC
Details for MytuD.01212.a.A1.GE25949
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL QALEGVVMEL DDCAFPLLSG VEIGSDPQKI FDGVSLALLV GARPRGAGME RSDLLEANGA IFTAQGKALN AVAADDVRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAISQL AAKTGAAVTD IKKMTIWGNH SATQYPDLFH AEVAGKNAAE VVNDQAWIED EFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PADDWVSMAV VSDGSYGVPE GLISSFPVTT KGGNWTIVSG LEIDEFSRGR IDKSTAEXXD ERSAVTELG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgctagtcc tctcaaggtc gccgttaccg gcgccgccgg ccaaatcggc tacagcctgt tgttccgcct ggccagcggc tctttgctgg gccctgaccg tccgatcgag ctgcggctgc tcgagatcga gccggcactg caggcgctcg agggtgtggt gatggaactc gacgactgcg ctttcccgct gttgtccggg gtggagatcg gttccgatcc ccagaagatc ttcgatggtg tgagcctggc cctgctggtc ggagcccgcc cccggggcgc gggcatggag cgaagtgacc tgctggaggc caacggcgcg atcttcaccg ctcagggcaa agccctcaac gctgtcgccg cggatgacgt tcgcgtcggg gtgaccggca accccgccaa caccaacgcg ctgatcgcga tgaccaatgc gcccgacatt ccccgcgagc ggttctcggc gctcacccgg ctggaccaca atcgggcgat ctcgcagctg gccgccaaga ccggcgcggc ggtcaccgac atcaagaaga tgacgatctg gggcaatcac tcggccaccc agtaccccga cctgttccac gcggaggtcg ccggaaagaa cgcggccgaa gtggtcaacg accaggcctg gatcgaggat gaattcatcc cgacggtcgc caagcgcggt gcggcgatca tcgatgcgcg cggcgcgtcg tcggccgcct cggccgcgtc ggcaaccatc gacgctgccc gggactggtt gctggggacg ccggcggacg attgggtctc gatggccgtc gtctccgacg ggtcctacgg ggtgccggag ggcttgatct cctcgtttcc ggtcaccacc aagggcggca actggacgat cgtgagcggc ttggagatcg acgagttctc ccgcggccgg atcgacaagt caaccgccga gttnncngac gagcgcagcg cggtcaccga gctcggcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL QALEGVVMEL DDCAFPLLSG VEIGSDPQKI FDGVSLALLV GARPRGAGME RSDLLEANGA IFTAQGKALN AVAADDVRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAISQL AAKTGAAVTD IKKMTIWGNH SATQYPDLFH AEVAGKNAAE VVNDQAWIED EFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PADDWVSMAV VSDGSYGVPE GLISSFPVTT KGGNWTIVSG LEIDEFSRGR IDKSTAELAD ERSAVTELGL I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgct agtcctctca aggtcgccgt taccggcgcc gccggccaaa tcggctacag cctgttgttc cgcctggcca gcggctcttt gctgggccct gaccgtccga tcgagctgcg gctgctcgag atcgagccgg cactgcaggc gctcgagggt gtggtgatgg aactcgacga ctgcgctttc ccgctgttgt ccggggtgga gatcggttcc gatccccaga agatcttcga tggtgtgagc ctggccctgc tggtcggagc ccgcccccgg ggcgcgggca tggagcgaag tgacctgctg gaggccaacg gcgcgatctt caccgctcag ggcaaagccc tcaacgctgt cgccgcggat gacgttcgcg tcggggtgac cggcaacccc gccaacacca acgcgctgat cgcgatgacc aatgcgcccg acattccccg cgagcggttc tcggcgctca cccggctgga ccacaatcgg gcgatctcgc agctggccgc caagaccggc gcggcggtca ccgacatcaa gaagatgacg atctggggca atcactcggc cacccagtac cccgacctgt tccacgcgga ggtcgccgga aagaacgcgg ccgaagtggt caacgaccag gcctggatcg aggatgaatt catcccgacg gtcgccaagc gcggtgcggc gatcatcgat gcgcgcggcg cgtcgtcggc cgcctcggcc gcgtcggcaa ccatcgacgc tgcccgggac tggttgctgg ggacgccggc ggacgattgg gtctcgatgg ccgtcgtctc cgacgggtcc tacggggtgc cggagggctt gatctcctcg tttccggtca ccaccaaggg cggcaactgg acgatcgtga gcggcttgga gatcgacgag ttctcccgcg gccggatcga caagtcaacc gccgagttgg ctgacgagcg cagcgcggtc accgagctcg gcctgatcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MytuD.01212.a.A1.PS00155
PURIFICATION DATe: 3/9/2009
CONCENTRATION: 36.51mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL QALEGVVMEL DDCAFPLLSG VEIGSDPQKI FDGVSLALLV GARPRGAGME RSDLLEANGA IFTAQGKALN AVAADDVRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAISQL AAKTGAAVTD IKKMTIWGNH SATQYPDLFH AEVAGKNAAE VVNDQAWIED EFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PADDWVSMAV VSDGSYGVPE GLISSFPVTT KGGNWTIVSG LEIDEFSRGR IDKSTAEXXD ERSAVTELG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgctagtcc tctcaaggtc gccgttaccg gcgccgccgg ccaaatcggc tacagcctgt tgttccgcct ggccagcggc tctttgctgg gccctgaccg tccgatcgag ctgcggctgc tcgagatcga gccggcactg caggcgctcg agggtgtggt gatggaactc gacgactgcg ctttcccgct gttgtccggg gtggagatcg gttccgatcc ccagaagatc ttcgatggtg tgagcctggc cctgctggtc ggagcccgcc cccggggcgc gggcatggag cgaagtgacc tgctggaggc caacggcgcg atcttcaccg ctcagggcaa agccctcaac gctgtcgccg cggatgacgt tcgcgtcggg gtgaccggca accccgccaa caccaacgcg ctgatcgcga tgaccaatgc gcccgacatt ccccgcgagc ggttctcggc gctcacccgg ctggaccaca atcgggcgat ctcgcagctg gccgccaaga ccggcgcggc ggtcaccgac atcaagaaga tgacgatctg gggcaatcac tcggccaccc agtaccccga cctgttccac gcggaggtcg ccggaaagaa cgcggccgaa gtggtcaacg accaggcctg gatcgaggat gaattcatcc cgacggtcgc caagcgcggt gcggcgatca tcgatgcgcg cggcgcgtcg tcggccgcct cggccgcgtc ggcaaccatc gacgctgccc gggactggtt gctggggacg ccggcggacg attgggtctc gatggccgtc gtctccgacg ggtcctacgg ggtgccggag ggcttgatct cctcgtttcc ggtcaccacc aagggcggca actggacgat cgtgagcggc ttggagatcg acgagttctc ccgcggccgg atcgacaagt caaccgccga gttnncngac gagcgcagcg cggtcaccga gctcggcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL QALEGVVMEL DDCAFPLLSG VEIGSDPQKI FDGVSLALLV GARPRGAGME RSDLLEANGA IFTAQGKALN AVAADDVRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAISQL AAKTGAAVTD IKKMTIWGNH SATQYPDLFH AEVAGKNAAE VVNDQAWIED EFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PADDWVSMAV VSDGSYGVPE GLISSFPVTT KGGNWTIVSG LEIDEFSRGR IDKSTAELAD ERSAVTELGL I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCT AGTCCTCTCA AGGTCGCCGT TACCGGCGCC GCCGGCCAAA TCGGCTACAG CCTGTTGTTC CGCCTGGCCA GCGGCTCTTT GCTGGGCCCT GACCGTCCGA TCGAGCTGCG GCTGCTCGAG ATCGAGCCGG CACTGCAGGC GCTCGAGGGT GTGGTGATGG AACTCGACGA CTGCGCTTTC CCGCTGTTGT CCGGGGTGGA GATCGGTTCC GATCCCCAGA AGATCTTCGA TGGTGTGAGC CTGGCCCTGC TGGTCGGAGC CCGCCCCCGG GGCGCGGGCA TGGAGCGAAG TGACCTGCTG GAGGCCAACG GCGCGATCTT CACCGCTCAG GGCAAAGCCC TCAACGCTGT CGCCGCGGAT GACGTTCGCG TCGGGGTGAC CGGCAACCCC GCCAACACCA ACGCGCTGAT CGCGATGACC AATGCGCCCG ACATTCCCCG CGAGCGGTTC TCGGCGCTCA CCCGGCTGGA CCACAATCGG GCGATCTCGC AGCTGGCCGC CAAGACCGGC GCGGCGGTCA CCGACATCAA GAAGATGACG ATCTGGGGCA ATCACTCGGC CACCCAGTAC CCCGACCTGT TCCACGCGGA GGTCGCCGGA AAGAACGCGG CCGAAGTGGT CAACGACCAG GCCTGGATCG AGGATGAATT CATCCCGACG GTCGCCAAGC GCGGTGCGGC GATCATCGAT GCGCGCGGCG CGTCGTCGGC CGCCTCGGCC GCGTCGGCAA CCATCGACGC TGCCCGGGAC TGGTTGCTGG GGACGCCGGC GGACGATTGG GTCTCGATGG CCGTCGTCTC CGACGGGTCC TACGGGGTGC CGGAGGGCTT GATCTCCTCG TTTCCGGTCA CCACCAAGGG CGGCAACTGG ACGATCGTGA GCGGCTTGGA GATCGACGAG TTCTCCCGCG GCCGGATCGA CAAGTCAACC GCCGAGTTGG CTGACGAGCG CAGCGCGGTC ACCGAGCTCG GCCTGATCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA