MymaA.01212.a

Malate dehydrogenase

CENTER ID: MymaA.01212.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01212.a.A1.GE30159 Full length( MymaA.01212.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4507
RefSeq: YP_001852461.1
UniProt: B2HRH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALKA LEGVVMELDD CAFPLLSGVE IGSDANKIFD GANLALLVGA RPRGPGMERS DLLEANGAIF TAQGKALNEV AADDIRVGVT GNPANTNALI AMTNAPDIPR ERFSALTRLD HNRAISQLAA KTGVAVTDIK KMTIWGNHSA TQYPDLFHAE VKGKNAAEVV NDQAWIEEYF IPTVAKRGAA IIDARGASSA ASAASATVDA ARSWLLGTPA DDWVSMAVLS DGSYGVPEGL ISSFPVTTKD GNWSIVKGLE IDEFSRGRID KTTAELADER KAVTELGLI
NT Sequence
GTGAGCGCAA GTCCCCTCAA GGTTGCCGTC ACCGGTGCCG CCGGCCAGAT CGGCTACAGC CTGTTGTTCC GCCTGGCCAG CGGCTCGCTG CTGGGCCCGG ATCGTCCGAT CGAGCTCCGC CTGCTCGAGA TCGAGCCGGC GCTCAAGGCG CTCGAGGGCG TCGTGATGGA ACTTGACGAC TGTGCGTTCC CGCTGCTGTC CGGCGTGGAG ATCGGCTCGG ACGCGAACAA GATTTTCGAC GGTGCGAATC TGGCCCTGCT CGTCGGCGCG CGTCCGCGCG GTCCGGGCAT GGAGCGCAGC GACCTGCTCG AGGCCAACGG CGCGATCTTC ACCGCTCAGG GTAAGGCGCT CAACGAGGTC GCCGCGGACG ACATCCGGGT GGGAGTGACC GGTAACCCGG CCAACACCAA CGCCCTGATC GCGATGACCA ACGCCCCCGA CATCCCCCGG GAGCGGTTCT CGGCGCTGAC CCGCCTGGAC CACAACCGCG CGATCTCGCA GCTGGCCGCC AAGACCGGTG TCGCGGTCAC CGACATCAAG AAGATGACCA TCTGGGGCAA CCACTCGGCC ACCCAGTACC CCGACCTTTT CCACGCCGAA GTCAAGGGCA AGAACGCGGC CGAGGTGGTC AATGACCAGG CGTGGATCGA GGAGTACTTC ATCCCGACCG TCGCCAAGCG TGGTGCGGCG ATCATCGACG CGCGCGGCGC ATCCTCGGCC GCTTCGGCCG CGTCGGCGAC CGTCGACGCA GCCCGTTCCT GGTTGCTCGG CACCCCCGCC GACGACTGGG TCTCGATGGC TGTCCTCTCC GACGGTTCCT ACGGTGTGCC CGAGGGCCTG ATCTCCTCGT TCCCGGTCAC CACCAAGGAC GGCAACTGGA GCATCGTCAA GGGCCTGGAG ATCGACGAAT TCTCCCGCGG TCGCATCGAC AAGACGACCG CTGAACTGGC CGACGAGCGC AAGGCGGTCA CCGAGCTCGG CCTGATC
Details for MymaA.01212.a.A1.GE30159
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL KALEGVVMEL DDCAFPLLSG VEIGSDANKI FDGANLALLV GARPRGPGME RSDLLEANGA IFTAQGKALN EVAADDIRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LXHNRAISQL AAKTGVAVTD IKKMTIWGNH SATQYPDLFH AEVKGKNAAX VVNDQAWIEE YFIPTVAXRG AAIIDARGAS SAASXASATV DAXRSWLLGT PADDWVSMAV LSXGSYXVPE G
Validated NT Sequence
tttgttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc gcaagtcccc tcaaggttgc cgtcaccggt gccgccggcc agatcggcta cagcctgttg ttccgcctgg ccagcggctc gctgctgggc ccggatcgtc cgatcgagct ccgcctgctc gagatcgagc cggcgctcaa ggcgctcgag ggcgtcgtga tggaacttga cgactgtgcg ttcccgctgc tgtccggcgt ggagatcggc tcggacgcga acaagatttt cgacggtgcg aatctggccc tgctcgtcgg cgcgcgtccg cgcggtccgg gcatggagcg cagcgacctg ctcgaggcca acggcgcgat cttcaccgct cagggtaagg cgctcaacga ggtcgccgcg gacgacatcc gggtgggagt gaccggtaac ccggccaaca ccaacgccct gatcgcgatg accaacgccc ccgacatccc ccgggagcgg ttctcggcgc tgacccgcct ggnccacaac cgcgcgatct cgcagctggc cgccaagacc ggtgtcgcgg tcaccgacat caagaagatg accatctggg gcaaccactc ggccacccag taccccgacc ttttccacgc cgaagtcaag ggcaagaacg cggccgangt ggtcaatgac caggcgtgga tcgaggagta cttcatcccg accgtcgccn agcgtggtgc ggcgatcatc gacgcgcgcg gcgcatcctc ggccgcttcg gncgcgtcgg cgaccgtcga cgcancccgt tcctggttgc tcggcacccc cgccgacgac tgggtctcga tggctgtcct ctccnanggt tcctacngtg tgcccgaggg ntgatcncct cgttccnnnt caccaccnnn nngnnanctg gnnnatcgtc nngggncctg gnnatcgacn aatnnnncnn nngncncatc gananacnac gctgaactgg nngacnnnnn nnngnnnnnn nannnngnnn nanctnnnnn acnanantnn ncnnnnncnn nnnngnncnn ngcnnnnnan nccnaagnnn ntnnnnnnnn ngnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL KALEGVVMEL DDCAFPLLSG VEIGSDANKI FDGANLALLV GARPRGPGME RSDLLEANGA IFTAQGKALN EVAADDIRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAISQL AAKTGVAVTD IKKMTIWGNH SATQYPDLFH AEVKGKNAAE VVNDQAWIEE YFIPTVAKRG AAIIDARGAS SAASAASATV DAARSWLLGT PADDWVSMAV LSDGSYGVPE GLISSFPVTT KDGNWSIVKG LEIDEFSRGR IDKTTAELAD ERKAVTELGL I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgca agtcccctca aggttgccgt caccggtgcc gccggccaga tcggctacag cctgttgttc cgcctggcca gcggctcgct gctgggcccg gatcgtccga tcgagctccg cctgctcgag atcgagccgg cgctcaaggc gctcgagggc gtcgtgatgg aacttgacga ctgtgcgttc ccgctgctgt ccggcgtgga gatcggctcg gacgcgaaca agattttcga cggtgcgaat ctggccctgc tcgtcggcgc gcgtccgcgc ggtccgggca tggagcgcag cgacctgctc gaggccaacg gcgcgatctt caccgctcag ggtaaggcgc tcaacgaggt cgccgcggac gacatccggg tgggagtgac cggtaacccg gccaacacca acgccctgat cgcgatgacc aacgcccccg acatcccccg ggagcggttc tcggcgctga cccgcctgga ccacaaccgc gcgatctcgc agctggccgc caagaccggt gtcgcggtca ccgacatcaa gaagatgacc atctggggca accactcggc cacccagtac cccgaccttt tccacgccga agtcaagggc aagaacgcgg ccgaggtggt caatgaccag gcgtggatcg aggagtactt catcccgacc gtcgccaagc gtggtgcggc gatcatcgac gcgcgcggcg catcctcggc cgcttcggcc gcgtcggcga ccgtcgacgc agcccgttcc tggttgctcg gcacccccgc cgacgactgg gtctcgatgg ctgtcctctc cgacggttcc tacggtgtgc ccgagggcct gatctcctcg ttcccggtca ccaccaagga cggcaactgg agcatcgtca agggcctgga gatcgacgaa ttctcccgcg gtcgcatcga caagacgacc gctgaactgg ccgacgagcg caaggcggtc accgagctcg gcctgatcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa