SSGCID: Target Page

MypaA.01212.a

Malate dehydrogenase

CENTER ID:	MypaA.01212.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01212.a.A1.GE29562	Full length( MypaA.01212.a )		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01212.a.A1.PW29460	Full length( MypaA.01212.a )		1	329

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.2697
RefSeq:	NP_961475.1
UniProt:	P61976

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALKA LEGVVMELDD CAFPLLSGVE IGADPNKIFD GANLALLVGA RPRGPGMERS DLLEANGAIF TAQGKALNEV AADDIRVGVT GNPANTNALI AMSNAPDIPR ERFSALTRLD HNRAISQLAK KTGAKVTDIK KMTIWGNHSA TQYPDIFHAE VKGKNAAEVV GDQNWIENDF IPTVAKRGAA IIDARGASSA ASAASATTDA ARDWLLGTPA GDWVSMAVIS DGSYGVPEGL ISSFPVTTKD GDWTIVQGLE IDEFSRSRID KTTAELADER NAVTQLGLI

NT Sequence

GTGAGCGCAA GTCCTCTCAA GGTTGCCGTC ACCGGCGCCG CCGGCCAGAT CGGCTACAGC CTGTTGTTCC GCCTGGCCAG CGGCTCGTTG CTGGGCCCCG ACCGCCCGAT CGAGCTGCGT CTGCTCGAGA TCGAGCCCGC GCTCAAGGCG CTCGAGGGCG TCGTGATGGA ACTCGACGAC TGCGCCTTCC CGCTGCTGTC CGGCGTCGAG ATCGGCGCCG ACCCGAACAA GATCTTCGAC GGCGCCAACC TCGCGCTGCT GGTCGGCGCG CGGCCGCGCG GCCCGGGCAT GGAGCGCAGC GACCTGCTGG AGGCCAACGG CGCGATCTTC ACCGCGCAGG GCAAGGCGCT CAACGAGGTG GCCGCCGACG ACATCCGCGT CGGCGTGACC GGCAACCCGG CCAACACCAA CGCGTTGATC GCGATGAGCA ACGCCCCGGA CATCCCGCGC GAGCGGTTCT CGGCGCTGAC CCGCCTGGAC CACAACCGGG CCATCTCGCA GCTGGCCAAA AAGACCGGCG CCAAGGTCAC CGACATCAAG AAGATGACGA TCTGGGGCAA CCACTCCGCC ACCCAGTACC CCGATATCTT CCACGCCGAG GTCAAGGGTA AGAACGCGGC CGAGGTGGTG GGCGACCAGA ACTGGATCGA GAACGACTTC ATCCCGACCG TGGCCAAGCG CGGCGCGGCC ATCATCGACG CGCGGGGCGC CTCCTCGGCC GCGTCGGCCG CCTCGGCGAC CACCGACGCC GCCCGCGACT GGCTGCTGGG CACCCCGGCG GGCGACTGGG TCTCCATGGC CGTCATCTCC GACGGCTCCT ACGGCGTGCC GGAGGGGCTG ATCTCGTCGT TCCCGGTGAC CACCAAGGAC GGCGACTGGA CCATCGTGCA GGGCCTGGAG ATCGACGAGT TCTCCCGCAG CCGGATCGAC AAGACCACCG CCGAGCTCGC CGACGAGCGC AACGCGGTGA CCCAGCTCGG CCTGATC

Details for MypaA.01212.a.A1.GE29562

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	108

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL KALEGVVMEL DDCAFPLLSG VEIGADPNKI FDGANLALLV GARPRGPGME RSDLLEANGA IFTAQGKALN EVAADDIRVG VTGNPANTNA LIAMSNAPDI PRERFSALTR LDHNRAISQL AKKTGAKVTD IKKMTIWGNH SATQYPDIFH AEVKGKNAAX VVGDQNWIEN DFIPTVAKRG AAIIDARGAS SAASAASATT DAARDWLLGT PAGDWXSMXR HLRRLLRRAG GADLVVPXDH QGRXXXXAXX XIDXXPXXXR XTXXXLXXXR XXXPXSXXXX XXXXXAXXXX XXXLXXXLXX XXXXXXXXXX

Validated NT Sequence

cnnnnnnnnt tttnnnnnnt ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc gcaagtcctc tcaaggttgc cgtcaccggc gccgccggcc agatcggcta cagcctgttg ttccgcctgg ccagcggctc gttgctgggc cccgaccgcc cgatcgagct gcgtctgctc gagatcgagc ccgcgctcaa ggcgctcgag ggcgtcgtga tggaactcga cgactgcgcc ttcccgctgc tgtccggcgt cgagatcggc gccgacccga acaagatctt cgacggcgcc aacctcgcgc tgctggtcgg cgcgcggccg cgcggcccgg gcatggagcg cagcgacctg ctggaggcca acggcgcgat cttcaccgcg cagggcaagg cgctcaacga ggtggccgcc gacgacatcc gcgtcggcgt gaccggcaac ccggccaaca ccaacgcgtt gatcgcgatg agcaacgccc cggacatccc gcgcgagcgg ttctcggcgc tgacccgcct ggaccacaac cgggccatct cgcagctggc caaaaagacc ggcgccaagg tcaccgacat caagaagatg acgatctggg gcaaccactc cgccacccag taccccgata tcttccacgc cgaggtcaag ggtaagaacg cggccgangt ggtgggcgac cagaactgga tcgagaacga cttcatcccg accgtggcca agcgcggcgc ggccatcatc gacgcgcggg gcgcctcctc ggccgcgtcg gccgcctcgg cgaccaccga cgccgcccgc gactggctgc tgggcacccc ggcgggcgac tgggnctcca tggnccgtca tctccgacgg ctcctacggc gtgccggagg ggctgatctc gtcgttccnn gngaccacca aggacggnna cngnatcntg cnnnnntggn natcgacnan nnnccncanc ngnatcgana nacnannncn nanctnnncn annancgcan nnnnnnaccc ngctcnnnnn ancnnnnann ngnacnanan tnngcannnn nntnnnnnnn nnnnnnnctn ncnnnnnnnc tnnnnnnnnn nnnnncncnn nnnnnnnnnn nnnnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL KALEGVVMEL DDCAFPLLSG VEIGADPNKI FDGANLALLV GARPRGPGME RSDLLEANGA IFTAQGKALN EVAADDIRVG VTGNPANTNA LIAMSNAPDI PRERFSALTR LDHNRAISQL AKKTGAKVTD IKKMTIWGNH SATQYPDIFH AEVKGKNAAE VVGDQNWIEN DFIPTVAKRG AAIIDARGAS SAASAASATT DAARDWLLGT PAGDWVSMAV ISDGSYGVPE GLISSFPVTT KDGDWTIVQG LEIDEFSRSR IDKTTAELAD ERNAVTQLGL I

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgca agtcctctca aggttgccgt caccggcgcc gccggccaga tcggctacag cctgttgttc cgcctggcca gcggctcgtt gctgggcccc gaccgcccga tcgagctgcg tctgctcgag atcgagcccg cgctcaaggc gctcgagggc gtcgtgatgg aactcgacga ctgcgccttc ccgctgctgt ccggcgtcga gatcggcgcc gacccgaaca agatcttcga cggcgccaac ctcgcgctgc tggtcggcgc gcggccgcgc ggcccgggca tggagcgcag cgacctgctg gaggccaacg gcgcgatctt caccgcgcag ggcaaggcgc tcaacgaggt ggccgccgac gacatccgcg tcggcgtgac cggcaacccg gccaacacca acgcgttgat cgcgatgagc aacgccccgg acatcccgcg cgagcggttc tcggcgctga cccgcctgga ccacaaccgg gccatctcgc agctggccaa aaagaccggc gccaaggtca ccgacatcaa gaagatgacg atctggggca accactccgc cacccagtac cccgatatct tccacgccga ggtcaagggt aagaacgcgg ccgaggtggt gggcgaccag aactggatcg agaacgactt catcccgacc gtggccaagc gcggcgcggc catcatcgac gcgcggggcg cctcctcggc cgcgtcggcc gcctcggcga ccaccgacgc cgcccgcgac tggctgctgg gcaccccggc gggcgactgg gtctccatgg ccgtcatctc cgacggctcc tacggcgtgc cggaggggct gatctcgtcg ttcccggtga ccaccaagga cggcgactgg accatcgtgc agggcctgga gatcgacgag ttctcccgca gccggatcga caagaccacc gccgagctcg ccgacgagcg caacgcggtg acccagctcg gcctgatcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MypaA.01212.a.A1.PW29460

PURIFICATION DATe:	8/23/2010
CONCENTRATION:	25.17mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	82
PERCENT COVERAGE:	108

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSASPLKV AVTGAAGQIG YSLLFRLASG SLLGPDRPIE LRLLEIEPAL KALEGVVMEL DDCAFPLLSG VEIGADPNKI FDGANLALLV GARPRGPGME RSDLLEANGA IFTAQGKALN EVAADDIRVG VTGNPANTNA LIAMSNAPDI PRERFSALTR LDHNRAISQL AKKTGAKVTD IKKMTIWGNH SATQYPDIFH AEVKGKNAAE VVGDQNWIEN DFIPTVAKRG AAIIDARGAS SAASAASATT DAARDWLLGT PAGDWVSMAV ISDGSYGVPE GLISSFPVTT KDGDWTIVQG LEIDEFSRSR IDKTTAELAD ERNAVTQLGL I

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCGCA AGTCCTCTCA AGGTTGCCGT CACCGGCGCC GCCGGCCAGA TCGGCTACAG CCTGTTGTTC CGCCTGGCCA GCGGCTCGTT GCTGGGCCCC GACCGCCCGA TCGAGCTGCG TCTGCTCGAG ATCGAGCCCG CGCTCAAGGC GCTCGAGGGC GTCGTGATGG AACTCGACGA CTGCGCCTTC CCGCTGCTGT CCGGCGTCGA GATCGGCGCC GACCCGAACA AGATCTTCGA CGGCGCCAAC CTCGCGCTGC TGGTCGGCGC GCGGCCGCGC GGCCCGGGCA TGGAGCGCAG CGACCTGCTG GAGGCCAACG GCGCGATCTT CACCGCGCAG GGCAAGGCGC TCAACGAGGT GGCCGCCGAC GACATCCGCG TCGGCGTGAC CGGCAACCCG GCCAACACCA ACGCGTTGAT CGCGATGAGC AACGCCCCGG ACATCCCGCG CGAGCGGTTC TCGGCGCTGA CCCGCCTGGA CCACAACCGG GCCATCTCGC AGCTGGCCAA AAAGACCGGC GCCAAGGTCA CCGACATCAA GAAGATGACG ATCTGGGGCA ACCACTCCGC CACCCAGTAC CCCGATATCT TCCACGCCGA GGTCAAGGGT AAGAACGCGG CCGAGGTGGT GGGCGACCAG AACTGGATCG AGAACGACTT CATCCCGACC GTGGCCAAGC GCGGCGCGGC CATCATCGAC GCGCGGGGCG CCTCCTCGGC CGCGTCGGCC GCCTCGGCGA CCACCGACGC CGCCCGCGAC TGGCTGCTGG GCACCCCGGC GGGCGACTGG GTCTCCATGG CCGTCATCTC CGACGGCTCC TACGGCGTGC CGGAGGGGCT GATCTCGTCG TTCCCGGTGA CCACCAAGGA CGGCGACTGG ACCATCGTGC AGGGCCTGGA GATCGACGAG TTCTCCCGCA GCCGGATCGA CAAGACCACC GCCGAGCTCG CCGACGAGCG CAACGCGGTG ACCCAGCTCG GCCTGATCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA