MyleA.01212.a

Malate dehydrogenase

CENTER ID: MyleA.01212.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01212.a.A1.GE34933 Full length( MyleA.01212.a ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1954
RefSeq: YP_002503429.1
UniProt: A0A0H3MQD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPRPLKVAV TGAAGQIGYS LLFRLASGSL LGLDRPIELR LLEIEPALKA LEGVVMELDD CAFLLLAGVE IGADPNKVFD GVNLALLVGA RPRGPGMERG DLLEANGAIF TAQGKALNAV AAADIRVGVT GNPANTNALI AMTNAPDIPR ERFSALTRLD HNRAIAQLAT KTGSAVTDIR KMTIWGNHSA TQYPDVFHAE IGGKNAAEVV GDQAWIEDYF IPTVAKRGAA IIDARGASSA ASAASATIDA ARDWLLGTPE GNWVSMAVVS DGSYGVPEGL ISSFPVTTTD GDWTIVRGLG IDDFSRGRID KSTAELADER MAVKQLGLI
NT Sequence
GTGAGCCCAC GTCCCCTCAA GGTCGCCGTA ACCGGCGCTG CCGGTCAGAT CGGTTATAGC CTGCTGTTCC GCTTGGCGAG CGGCTCACTG CTGGGGCTTG ACCGCCCGAT TGAGTTGCGT CTGCTTGAGA TTGAGCCCGC GCTCAAGGCG CTCGAGGGTG TCGTGATGGA ACTTGATGAC TGCGCGTTCC TGCTGTTGGC GGGCGTCGAG ATAGGCGCGG ATCCGAACAA GGTCTTCGAC GGTGTCAACC TGGCCCTGCT TGTCGGTGCC CGGCCGCGCG GGCCGGGCAT GGAACGTGGC GATTTATTGG AGGCTAACGG CGCGATATTC ACTGCGCAGG GCAAGGCCCT CAACGCCGTC GCCGCCGCTG ACATCCGTGT CGGTGTGACT GGCAATCCGG CCAACACCAA CGCGCTGATC GCGATGACCA ACGCGCCCGA CATCCCCCGC GAGCGGTTCT CGGCACTTAC CCGTCTGGAC CACAACCGGG CTATTGCGCA GCTAGCCACG AAGACCGGCT CCGCAGTCAC CGACATCAGG AAGATGACGA TCTGGGGCAA CCACTCGGCC ACCCAATACC CCGACGTTTT CCACGCTGAG ATCGGCGGTA AGAACGCGGC CGAGGTCGTG GGGGACCAAG CTTGGATCGA GGACTACTTT ATCCCGACCG TCGCCAAGCG CGGTGCTGCG ATCATCGATG CACGCGGCGC TTCGTCAGCC GCTTCGGCAG CATCGGCGAC CATTGATGCC GCCCGGGACT GGCTGCTGGG CACCCCTGAG GGCAACTGGG TTTCGATGGC GGTGGTATCC GATGGTTCGT ACGGTGTGCC GGAGGGCCTG ATCTCTTCGT TCCCTGTAAC CACCACGGAT GGAGACTGGA CGATCGTCAG GGGCTTGGGG ATCGATGACT TCTCCCGCGG GCGGATCGAC AAGTCGACCG CAGAGCTGGC CGACGAGCGT ATGGCGGTCA AGCAGCTCGG TCTGATT
Details for MyleA.01212.a.A1.GE34933
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSPRPLKV AVTGAAGQIG YSLLFRLASG SLLGLDRPIE LRLLEIEPAL KALEGVVMEL DDCAFLLLAG VEIGADPNKV FDGVNLALLV GARPRGPGME RGDLLEANGA IFTAQGKALN AVAAADIRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAIAQL ATKTGSAVTD IRKMTIWGNH SATQYPDVFH AEIGGKNAAX VVGDQAWIED YFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PEGNWVSMAV VSDGSXGVPE GLISSFPXTX TXGXWXIVXX LGXDDXXRXX IXXSTXXLXX XXMAXKXLGX IXXXXXXXXX
Validated NT Sequence
ttntttanct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc ccacgtcccc tcaaggtcgc cgtaaccggc gctgccggtc agatcggtta tagcctgctg ttccgcttgg cgagcggctc actgctgggg cttgaccgcc cgattgagtt gcgtctgctt gagattgagc ccgcgctcaa ggcgctcgag ggtgtcgtga tggaacttga tgactgcgcg ttcctgctgt tggcgggcgt cgagataggc gcggatccga acaaggtctt cgacggtgtc aacctggccc tgcttgtcgg tgcccggccg cgcgggccgg gcatggaacg tggcgattta ttggaggcta acggcgcgat attcactgcg cagggcaagg ccctcaacgc cgtcgccgcc gctgacatcc gtgtcggtgt gactggcaat ccggccaaca ccaacgcgct gatcgcgatg accaacgcgc ccgacatccc ccgcgagcgg ttctcggcac ttacccgtct ggaccacaac cgggctattg cgcagctagc cacgaagacc ggctccgcag tcaccgacat caggaagatg acgatctggg gcaaccactc ggccacccaa taccccgacg ttttccacgc tgagatcggc ggtaagaacg cggccgangt cgtgggggac caagcttgga tcgaggacta ctttatcccg accgtcgcca agcgcggtgc tgcgatcatc gatgcacgcg gcgcttcgtc agccgcttcg gcagcatcgg cgaccattga tgccgcccgg gactggctgc tgggcacccc tgagggcaac tgggtttcga tggcggtggt atccgatggt tcgtanggng tgccggaggg cctgatctct tcgttccctg naaccnncac ggangganac tggncnatcg tcngggnctt ggggntcgat gacttnnncc gcnggcngat cnannagtcn accnnnnanc tngncnanna ncnnatggcg gncaagnnnc tnggnnngat tnnnnaaann nnnnnannan ttntnnanc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSPRPLKV AVTGAAGQIG YSLLFRLASG SLLGLDRPIE LRLLEIEPAL KALEGVVMEL DDCAFLLLAG VEIGADPNKV FDGVNLALLV GARPRGPGME RGDLLEANGA IFTAQGKALN AVAAADIRVG VTGNPANTNA LIAMTNAPDI PRERFSALTR LDHNRAIAQL ATKTGSAVTD IRKMTIWGNH SATQYPDVFH AEIGGKNAAE VVGDQAWIED YFIPTVAKRG AAIIDARGAS SAASAASATI DAARDWLLGT PEGNWVSMAV VSDGSYGVPE GLISSFPVTT TDGDWTIVRG LGIDDFSRGR IDKSTAELAD ERMAVKQLGL I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagccca cgtcccctca aggtcgccgt aaccggcgct gccggtcaga tcggttatag cctgctgttc cgcttggcga gcggctcact gctggggctt gaccgcccga ttgagttgcg tctgcttgag attgagcccg cgctcaaggc gctcgagggt gtcgtgatgg aacttgatga ctgcgcgttc ctgctgttgg cgggcgtcga gataggcgcg gatccgaaca aggtcttcga cggtgtcaac ctggccctgc ttgtcggtgc ccggccgcgc gggccgggca tggaacgtgg cgatttattg gaggctaacg gcgcgatatt cactgcgcag ggcaaggccc tcaacgccgt cgccgccgct gacatccgtg tcggtgtgac tggcaatccg gccaacacca acgcgctgat cgcgatgacc aacgcgcccg acatcccccg cgagcggttc tcggcactta cccgtctgga ccacaaccgg gctattgcgc agctagccac gaagaccggc tccgcagtca ccgacatcag gaagatgacg atctggggca accactcggc cacccaatac cccgacgttt tccacgctga gatcggcggt aagaacgcgg ccgaggtcgt gggggaccaa gcttggatcg aggactactt tatcccgacc gtcgccaagc gcggtgctgc gatcatcgat gcacgcggcg cttcgtcagc cgcttcggca gcatcggcga ccattgatgc cgcccgggac tggctgctgg gcacccctga gggcaactgg gtttcgatgg cggtggtatc cgatggttcg tacggtgtgc cggagggcct gatctcttcg ttccctgtaa ccaccacgga tggagactgg acgatcgtca ggggcttggg gatcgatgac ttctcccgcg ggcggatcga caagtcgacc gcagagctgg ccgacgagcg tatggcggtc aagcagctcg gtctgattaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa